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Antibodies

Histone antibodies

Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A nucleosome consists of one pair of each of the core histones (H2A, H2B, H3 and H4) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone H1, allowing for further condensation of chromatin.

The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome.  They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These post-translational modifications include  methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation.  The most well characterized PTMs are methylation, acetylation and phosphorylation. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).

The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with euchromatin and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in heterochromatin and associated with gene silencing. The combination of different histone modifications is called the "histone code”, analogous to the genetic code.

Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our  antibodies are highly specific and have been validated in many applications, including ChIP and ChIP-seq.

Diagenode’s collection includes antibodies recognizing:

  • Histone H1 variants
  • Histone H2A, H2A variants and histone H2A modifications (serine phosphorylation, lysine acetylation, lysine ubiquitinylation)
  • Histone H2B and H2B modifications (serine phosphorylation, lysine acetylation)
  • Histone H3 and H3 modifications (lysine methylation (mono-, di- and tri-methylated), lysine acetylation, serine phosphorylation, threonine phosphorylation, arginine methylation (mono-methylated, symmetrically and asymmetrically di-methylated))
  • Histone H4 and H4 modifications (lysine methylation (mono-, di- and tri-methylated), lysine acetylation, arginine methylation (mono-methylated and symmetrically di-methylated), serine phosphorylation )

HDAC's HAT's, HMT's and other enzymes which modify histones can be found in the category Histone modifying enzymes

Diagenode’s highly validated antibodies:

  •         Highly sensitive and specific
  •         Cost-effective (requires less antibody per reaction)
  •         Batch-specific data is available on the website
  •         Expert technical support
  •         Sample sizes available
  •        100% satisfaction guarantee

Products

Cat. No.ProductApplicationPurity
C15410316-50 H3R2me2(asym) Antibody
(50 μg /50 μl) 		ChIP-qPCR WB Affinity purified
C15310224 H1.0 Antibody
(100 µl) 		ELISA Whole antiserum
C15310249 H1.1 Antibody
(100 µl) 		ELISA Whole antiserum
C15310226 H1.3 Antibody
(100 µl) 		ELISA Whole antiserum
C15310227 H1.4 Antibody
(100 µl) 		ELISA Whole antiserum
C15310228 H1.5 Antibody
(100 µl) 		ELISA Whole antiserum
C15210002 H2A.X Antibody
(100 µg /100 µl) 		ChIP-qPCR IF WB Protein A purified
C15410219 H2A.XS139p Antibody
(50 μg/ 19 μl) 		DB ELISA IF WB Affinity purified
C15310223 H2A.XS139p Antibody
(100 µl) 		ELISA Whole antiserum
C15410201 H2A.Z Antibody
(50 μg) 		ChIP-qPCR ChIP-seq CUT&Tag ELISA IF WB Affinity purified
C15210021 H2A.Z Antibody
(100 µg) 		ChIP-seq IF WB Affinity purified polyclonal antibody
C15410173 H2A.Zac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF Affinity purified
C15410202-50 H2A.Zac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15210009 H2A.ZK4ac Antibody
(100 µg) 		ChIP-qPCR ChIP-seq IF WB Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
C15210012 H2A.ZK7ac Antibody
(100 μg) 		ChIP-qPCR ChIP-seq IF WB Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
C15410002 H2AK119ub Antibody
(50 μg/46 μl) 		ChIP-qPCR DB ELISA WB Affinity purified
C15410215 H2AK5ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410166 H2Apan Antibody
(50 μg) 		ChIP-qPCR ELISA IF WB Affinity purified
C15410212 H2BK12ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410220 H2BK15ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15210010 H2BK20ac Antibody
(100 µg) 		ChIP-qPCR ChIP-seq IF WB Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
C15410157 H2Bpan Antibody
(50 μg/18 μl) 		ChIP-qPCR ELISA WB Peptide affinity purified
C15200186 H2Bs14p Antibody
(50 μg/50 μl) 		DB ELISA Protein A purified
C15210011 H3.3 Antibody
(100 µg) 		ChIP-qPCR ChIP-seq WB Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
C15410310 H3K14ac Antibody
(50 μg) 		ChIP-qPCR DB ELISA WB Affinity purified
C15210005 H3K14ac Antibody
(100 µg) 		ChIP-qPCR ChIP-seq DB IF WB Protein A purified monoclonal antibody in PBS containing 1% BSA and 0.09% azide.
C15410139 H3K18ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15210020 H3K18ac Antibody
(100 µg) 		ChIP-qPCR ChIP-seq DB IF WB Affinity purified monoclonal antibody
C15410290 H3K18me1 Antibody
(50 μg) 		ChIP-qPCR DB IF WB Affinity purified
C15410291 H3K18me2 Antibody
(50 μg) 		ChIP-qPCR DB IF WB Affinity purified
C15410292 H3K18me3 Antibody
(50 μg) 		ChIP-qPCR DB IC IF WB Affinity purified
C15410344 H3K23ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified polyclonal antibody in PBS containing 0.05% azide.
C15210007 H3K23me2 Antibody
(100 µg) 		ChIP-qPCR ChIP-seq WB Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
C15200184-50 H3K27ac Antibody
(50 μg) 		ChIP-qPCR CUT&Tag ELISA IF WB Protein A purified
C15410174 H3K27ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified polyclonal antibody.
C15410196 H3K27ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB Affinity purified
C15210016 H3K27ac Antibody
(100 µg) 		ChIP-qPCR DB IF WB Affinity purified polyclonal antibody.
C15410045-50 H3K27me1 Antibody
(50 µg) 		ChIP-qPCR DB ELISA IF WB Affinity purified polyclonal antibody.
C15410046-50 H3K27me2 Antibody
(50 µg/19 µl) 		ChIP-qPCR DB ELISA IF WB Affinity purified
C15200181-50 H3K27me3 Antibody
(50 μg) 		ChIP-qPCR CUT&Tag ELISA IF WB Protein A purified monoclonal antibody.
C15410069 H3K27me3 Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified polyclonal antibody.
C15410195 H3K27me3 Antibody
(50 μg) 		ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF Peptide array WB Affinity purified polyclonal antibody.
C15310091 H3K27me3S28p Antibody
(100 µl) 		ChIP-qPCR DB ELISA IF IP WB Whole antiserum
C15410293 H3K27me3S28p Antibody
(50 μg) 		ChIP-qPCR DB IF WB Affinity purified
C15410091 H3K27me3S28p Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
C15410307 H3K36ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
C15410089 H3K36me1 Antibody
(50 µg/54 µl) 		ChIP-qPCR DB ELISA WB Affinity purified
C15200182 H3K36me2 Antibody
(50 μg/50 μl) 		ChIP-qPCR DB ELISA WB Protein A purified monoclonal antibody.
C15310127 H3K36me2 Antibody
(100 µl) 		ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB Whole antiserum from rabbit.
C15200183 H3K36me3 Antibody
(50 μg/50 μl) 		ChIP-qPCR DB ELISA IF WB Protein A purified
C15310058 H3K36me3 Antibody
(100 µl) 		ChIP-qPCR ChIP-seq DB ELISA WB Whole antiserum from rabbit containing 0.05% azide.
C15410058 H3K36me3 Antibody
(50 µg) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
C15410192 H3K36me3 Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified polyclonal antibody.
C15210013 H3K36me3 Antibody
(100 µg) 		ChIP-qPCR ChIP-seq DB WB Affinity purified polyclonal antibody.
C15410295 H3K37me1 Antibody
(50 μg) 		DB WB Affinity purified
C15410322 H3K4ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified polyclonal antibody
C15410165 H3K4ac Antibody - replaced by the reference C15410322
(50 μg/34 μl) 		ChIP-qPCR DB ELISA IF WB Affinity purified
C15200150 H3K4me1 Antibody
(50 µg/50 µl) 		ChIP-qPCR ELISA IF WB Protein A purified
C15310037 H3K4me1 Antibody
(100 µl) 		ChIP-qPCR DB ELISA WB Whole antiserum
C15410037-50 H3K4me1 Antibody
(50 µg) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified polyclonal antibody.
C15410194 H3K4me1 Antibody
(50 μg) 		ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF Peptide array WB Affinity purified polyclonal antibody.
C15210018 H3K4me1 Antibody
(100 µg) 		ChIP-qPCR ChIP-seq DB IF WB Affinity purified polyclonal antibody.
C15410280 H3K4me1T6p Antibody
(50 μg) 		DB IF IHC WB Affinity purified
C15200151 H3K4me2 Antibody
(50 µg/50 µl) 		ChIP-qPCR ELISA IF WB Protein A purified
C15310035 H3K4me2 Antibody
(100 µl) 		ChIP-qPCR DB ELISA WB Whole antiserum
C15410035 H3K4me2 Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified polyclonal antibody.
C15200152 H3K4me3 Antibody
(50 µg) 		ChIP-qPCR ChIP-seq ELISA IF WB Protein A purified monoclonal antibody.
C15410030 H3K4me3 Antibody
(50 µg) 		ChIP-qPCR ChIP-seq DB IF WB Affinity purified polyclonal antibody.
C15310003 H3K4me3 Antibody
(100 µl) 		ChIP-qPCR DB ELISA WB Whole antiserum
C15410003-50 H3K4me3 Antibody
(50 µg) 		ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB Affinity purified polyclonal antibody.
C15410281 H3K4me3T6p Antibody
(50 μg) 		ChIP-qPCR DB IF IHC WB Affinity purified
C15200149 H3K4un Antibody
(50 µg/50 µl) 		ChIP-qPCR ELISA IF WB Protein A purified
C15410213 H3K56ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq CUT&Tag ELISA WB Affinity purified
C15410296 H3K56me1 Antibody
(50 μg) 		ChIP-qPCR DB IF WB Affinity purified
C15410297 H3K56me3 Antibody
(50 μg) 		DB IF WB Affinity purified
C15410211 H3K64me3 Antibody
(50 μg) 		ChIP-qPCR DB ELISA WB Affinity purified
C15210006 H3K79ac Antibody
(100 µg) 		ChIP-qPCR ChIP-seq IF WB Protein A purified
C15310082 H3K79me1 Antibody
(100 µl) 		ChIP-qPCR DB ELISA WB Whole antiserum
C15410082 H3K79me1 Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
C15310051 H3K79me2 Antibody
(100 µl) 		ChIP-qPCR DB ELISA WB Whole antiserum
C15410051 H3K79me2 Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15310068 H3K79me3 Antibody
(100 µl) 		DB ELISA IF WB Whole antiserum
C15410068 H3K79me3 Antibody
(50 µg) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified polyclonal antibody
C15410005 H3K9/14ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410200 H3K9/14ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF Peptide array WB Affinity purified polyclonal antibody.
C15200185-50 H3K9ac Antibody
(50 μg/50 μl) 		ChIP-qPCR ELISA IF WB Protein A purified monoclonal antibody
C15410004 H3K9ac Antibody
(50 µg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410177 H3K9ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
C15210015 H3K9ac Antibody
(100 µg) 		ChIP-qPCR ChIP-seq DB WB Affinity purified polyclonal antibody.
C15310102 H3K9acS10p Antibody
(100 µl) 		ChIP-qPCR DB ELISA IF WB Whole antiserum
C15310065 H3K9me1 Antibody
(100 µl) 		ChIP-qPCR DB ELISA IF WB Whole antiserum
C15410065 H3K9me1 Antibody
(50 µg/43 µl) 		ChIP-qPCR DB ELISA WB Affinity purified
C15200154 H3K9me2 Antibody
(50 μg) 		ChIP-qPCR ELISA IF WB Protein A purified monoclonal antibody.
C15410060 H3K9me2 Antibody
(50 µg/44 µl) 		ChIP-qPCR DB ELISA IF WB Affinity purified polyclonal antibody
C15210019 H3K9me2 Antibody
(100 µg/ 100 µl) 		ChIP-qPCR ChIP-seq IF WB Affinity purified monoclonal antibody
C15200146 H3K9me3 Antibody
(50 μg) 		ChIP-qPCR DB IF WB Protein A purified
C15410013 H3K9me3 Antibody
(50 µg/39 µl) 		WB Protein G purified
C15100146 H3K9me3 Antibody
(100 µl) 		ChIP-qPCR DB WB Concentrated supernatant
C15410056 H3K9me3 Antibody
(50 µg) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified polyclonal antibody.
C15310013 H3K9me3 Antibody
(100 µl) 		ChIP-qPCR WB Whole antiserum
C15410193 H3K9me3 Antibody
(50 μg) 		ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB Affinity purified polyclonal antibody.
C15210014 H3K9me3 Antibody
(100 µg) 		ChIP-qPCR ChIP-seq WB Affinity purified polyclonal antibody.
C15200153 H3K9me3 Antibody
(50 µg/50 µl) 		ChIP-qPCR ELISA IF WB Protein A purified
C15500003-50 H3K9me3 recombinant Antibody
(50 µg) 		ChIP-qPCR ChIP-seq IF affinity purified
C15310128 H3K9me3S10p Antibody
(100 µl) 		ChIP-qPCR DB ELISA IF WB Whole antiserum
C15200187 H3K9un Antibody
(50 μg/25 μl) 		ChIP-qPCR IF WB Protein A purified
C15310135 H3pan Antibody
(100 µl) 		ChIP-qPCR ELISA WB Whole antiserum
C15410324-50 H3pan Antibody
(50 μg/16 μl) 		ChIP-qPCR ELISA IF WB Affinity purified
C15200011 H3pan Antibody 1B1B2
(50 μg) 		ChIP-qPCR IF WB Protein A purified monoclonal antibody.
C15310092 H3R17me2(asym) Antibody
(100 µl) 		ChIP-qPCR DB ELISA WB Whole antiserum
C15410289 H3R17me2(asym) Antibody
(50 μg) 		ChIP-qPCR IF WB Affinity purified
C15410171 H3R17me2(asym)K18ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410276 H3R2me1 Antibody
(50 μg) 		WB Affinity purified
C15410294 H3R2me2K4me2 Antibody
(50 μg) 		ChIP-qPCR DB IF WB Affinity purified
C15410303 H3R2me2sT3p Antibody
(50 μg) 		DB IF WB Affinity purified
C15410286 H3R8me2(asym) Antibody
(50 μg) 		ChIP-qPCR IF IHC WB Affinity purified
C15410287 H3R8me2(sym) Antibody
(50 μg) 		ChIP-qPCR WB Affinity purified
C15310116 H3S10p Antibody
(100 µl) 		ChIP-qPCR DB ELISA IF IP WB Whole antiserum
C15410116-50 H3S10p Antibody
(50 μg/48 μl) 		ChIP-qPCR DB ELISA IF WB Affinity purified
C15410288 H3S10pT11p Antibody
(50 μg) 		ChIP-qPCR IF IHC WB Affinity purified
C15410309 H3T11p Antibody
(50 μg) 		ChIP-qPCR DB ELISA IF WB Affinity purified
C15210001 H3T3p Antibody
(100 μg/100 μl) 		ChIP-qPCR IF WB Protein A purified
C15410279 H3T3pK4ac Antibody
(50 μg) 		DB IF IHC WB Affinity purified
C15410277 H3T3pK4me1 Antibody
(50 μg) 		DB IF IHC WB Affinity purified
C15410278 H3T3pK4me2 Antibody
(50 μg) 		ChIP-qPCR DB IF IHC WB Affinity purified
C15410321 H3T45p Antibody
(50 μg /34 μl) 		DB ELISA Affinity purified
C15410282 H3T6p Antibody
(50 μg) 		DB IF IHC WB Affinity purified
C15410283 H3T6pK9me1 Antibody
(50 μg) 		ChIP-qPCR DB IF IHC WB Affinity purified
C15410284 H3T6pK9me2 Antibody
(50 μg) 		ChIP-qPCR IF IHC WB Affinity purified
C15410285 H3T6pK9me3 Antibody
(50 μg) 		ChIP-qPCR DB IF IHC WB Affinity purified
C15410302 H4K12ac Antibody
(50 μg) 		DB IF IHC WB Affinity purified
C15200218 H4K12ac Antibody
(50 µg) 		ChIP-qPCR DB WB Protein A purified
C15410331-50 H4K12ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15200219 H4K16ac Antibody
(50 µg) 		ChIP-qPCR DB WB Protein A purified
C15410300 H4K16ac Antibody
(50 μg) 		DB IF WB Affinity purified
C15210008 H4K20ac Antibody
(100 µg) 		ChIP-qPCR ChIP-seq IF WB Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
C15200147 H4K20me1 Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB IF WB Protein A purified
C15310034 H4K20me1 Antibody
(100 µl) 		ChIP-qPCR DB ELISA WB Whole antiserum
C15410034 H4K20me1 Antibody
(50 μg/25 μl) 		ChIP-qPCR DB ELISA IF WB Affinity purified
C15100147 H4K20me1 Antibody
(100 µl) 		ChIP-qPCR DB WB Concentrated supernatant
C15200205 H4K20me2 Antibody
(100 µl) 		ChIP-qPCR IC IF WB Protein G purified
C15200220 H4K20me2 Antibody
(50 µg) 		ChIP-qPCR DB IF WB Protein A purified
C15410301 H4K20me2 Antibody
(50 μg) 		ChIP-qPCR IF WB Affinity purified
C15100148 H4K20me3 Antibody
(100 µl) 		ChIP-qPCR WB Concentrated supernatant
C15410057 H4K20me3 Antibody
(50 μg) 		ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB Affinity purified
C15410207 H4K20me3 Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA IF Peptide array WB Affinity purified polyclonal antibody.
C15410024 H4K5,8,12,16ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq DB IF Affinity purified polyclonal antibody.
C15410021 H4K5,8,12ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB Affinity purified
C15410025 H4K5ac Antibody
(50 μg) 		ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB Affinity purified
C15200217 H4K8ac Antibody
(50 µg) 		ChIP-qPCR DB WB Protein A purified
C15310103 H4K8ac Antibody
(100 µl) 		ChIP-qPCR DB ELISA WB Whole antiserum
C15410103 H4K8ac Antibody
(50 μg/41 μl) 		ChIP-qPCR DB ELISA IF WB Affinity purified
C15410156-50 H4pan Antibody
(50 μg/39 μl) 		ChIP-qPCR ELISA WB Affinity purified
C15410299 H4R3me1 Antibody
(50 μg) 		ChIP-qPCR DB IC IF WB Affinity purified
C15210022 H4R3me1 Antibody
(100 µg) 		DB IF WB Affinity purified monoclonal antibody
C15410308 H4R3me2(sym) Antibody
(50 μg) 		DB ELISA IF WB Affinity purified
C15410298 H4S1p Antibody
(50 μg) 		ChIP-qPCR DB IF IHC WB Affinity purified
C15210004 H4S1p Antibody
(100 µg /100 µl) 		ChIP-qPCR IF WB Protein A purified
C15210003 macroH2A.1/H2A.2 Antibody
(100 µg) 		ChIP-qPCR ChIP-seq IF WB Protein A purified
C15200232-50 yeast H2BK123ub Antibody
(50 μg/ 50 μl) 		ChIP-qPCR IP WB Affinity purified monoclonal antibody in PBS containing 0.02% azide.

Events

  • Virtual ChIP workshop - January 18-19, 2022 or January 25-26, 2022
    Diagenode
    Jan 18-Jan 26, 2022
  • Virtual DNA Methylation - January 18-19, 2022 or January 25-26, 2022
    Diagenode
    Jan 18-Jan 26, 2022
  • Lausanne Genomics Days 2022
    Lausanne, Switzerland
    Feb 14-Feb 15, 2022
 See all events

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