Histones are the main protein components of chromatin involved in the compaction of DNA into nucleosomes, the basic units of chromatin. A nucleosome consists of one pair of each of the core histones (H2A, H2B, H3 and H4) forming an octameric structure wrapped by 146 base pairs of DNA. The different nucleosomes are linked by the linker histone H1, allowing for further condensation of chromatin.
The core histones have a globular structure with large unstructured N-terminal tails protruding from the nucleosome. They can undergo to multiple post-translational modifications (PTM), mainly at the N-terminal tails. These post-translational modifications include methylation, acetylation, phosphorylation, ubiquitinylation, citrullination, sumoylation, deamination and crotonylation. The most well characterized PTMs are methylation, acetylation and phosphorylation. Histone methylation occurs mainly on lysine (K) residues, which can be mono-, di- or tri-methylated, and on arginines (R), which can be mono-methylated and symmetrically or asymmetrically di-methylated. Histone acetylation occurs on lysines and histone phosphorylation mainly on serines (S), threonines (T) and tyrosines (Y).
The PTMs of the different residues are involved in numerous processes such as DNA repair, DNA replication and chromosome condensation. They influence the chromatin organization and can be positively or negatively associated with gene expression. Trimethylation of H3K4, H3K36 and H3K79, and lysine acetylation generally result in an open chromatin configuration (figure below) and are therefore associated with euchromatin and gene activation. Trimethylation of H3K9, K3K27 and H4K20, on the other hand, is enriched in heterochromatin and associated with gene silencing. The combination of different histone modifications is called the "histone code”, analogous to the genetic code.
Diagenode is proud to offer a large range of antibodies against histones and histone modifications. Our antibodies are highly specific and have been validated in many applications, including ChIP and ChIP-seq.
Diagenode’s collection includes antibodies recognizing:
HDAC's HAT's, HMT's and other enzymes which modify histones can be found in the category Histone modifying enzymes
Diagenode’s highly validated antibodies:
Cat. No. | Product | Application | Purity | |
---|---|---|---|---|
C15310224 |
H1.0 Antibody (100 µl) |
ELISA | Whole antiserum | |
C15310249 |
H1.1 Antibody (100 µl) |
ELISA | Whole antiserum | |
C15310226 |
H1.3 Antibody (100 µl) |
ELISA | Whole antiserum | |
C15310227 |
H1.4 Antibody (100 µl) |
ELISA | Whole antiserum | |
C15310228 |
H1.5 Antibody (100 µl) |
ELISA | Whole antiserum | |
C15210002 |
H2A.X Antibody (100 µg /100 µl) |
ChIP-qPCR IF WB | Protein A purified | |
C15410219 |
H2A.XS139p Antibody (50 μg/ 19 μl) |
DB ELISA IF WB | Affinity purified | |
C15310223 |
H2A.XS139p Antibody (100 µl) |
ELISA | Whole antiserum | |
C15410201 |
H2A.Z Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag ELISA IF WB | Affinity purified | |
C15210021 |
H2A.Z Antibody (100 µg) |
ChIP-seq IF WB | Affinity purified polyclonal antibody | |
C15410173 |
H2A.Zac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF | Affinity purified | |
C15410202-50 |
H2A.Zac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
C15210009 |
H2A.ZK4ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq IF WB | Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. | |
C15210012 |
H2A.ZK7ac Antibody (100 μg) |
ChIP-qPCR ChIP-seq IF WB | Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. | |
C15410002 |
H2AK119ub Antibody (50 μg/46 μl) |
ChIP-qPCR DB ELISA WB | Affinity purified | |
C15410215 |
H2AK5ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
C15410166 |
H2Apan Antibody (50 μg) |
ChIP-qPCR ELISA IF WB | Affinity purified | |
C15410212 |
H2BK12ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
C15410220 |
H2BK15ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
C15210010 |
H2BK20ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq IF WB | Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. | |
C15410157 |
H2Bpan Antibody (50 μg/18 μl) |
ChIP-qPCR ELISA WB | Peptide affinity purified | |
C15200186 |
H2Bs14p Antibody (50 μg/50 μl) |
DB ELISA | Protein A purified | |
C15210011 |
H3.3 Antibody (100 µg) |
ChIP-qPCR ChIP-seq WB | Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. | |
C15410310 |
H3K14ac Antibody (50 μg) |
ChIP-qPCR DB ELISA WB | Affinity purified | |
C15210005 |
H3K14ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq DB IF WB | Protein A purified monoclonal antibody in PBS containing 1% BSA and 0.09% azide. | |
C15410139 |
H3K18ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified | |
C15210020 |
H3K18ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq DB IF WB | Affinity purified monoclonal antibody | |
C15410290 |
H3K18me1 Antibody (50 μg) |
ChIP-qPCR DB IF WB | Affinity purified | |
C15410291 |
H3K18me2 Antibody (50 μg) |
ChIP-qPCR DB IF WB | Affinity purified | |
C15410344 |
H3K23ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified polyclonal antibody in PBS containing 0.05% azide. | |
C15210007 |
H3K23me2 Antibody (100 µg) |
ChIP-qPCR ChIP-seq WB | Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. | |
C15200184-50 |
H3K27ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag ELISA IF WB | Protein A purified | |
C15410174 |
H3K27ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified polyclonal antibody. | |
C15410196 |
H3K27ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified | |
C15210016 |
H3K27ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq DB IF WB | Affinity purified polyclonal antibody. | |
C15410045-50 |
H3K27me1 Antibody (50 µg) |
ChIP-qPCR DB ELISA IF WB | Affinity purified polyclonal antibody. | |
C15410046-50 |
H3K27me2 Antibody (50 µg/19 µl) |
ChIP-qPCR DB ELISA IF WB | Affinity purified | |
C15200181-50 |
H3K27me3 Antibody (50 μg) |
ChIP-qPCR CUT&Tag ELISA IF WB | Protein A purified monoclonal antibody. | |
C15410069 |
H3K27me3 Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified polyclonal antibody. | |
C15410195 |
H3K27me3 Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF Peptide array WB | Affinity purified polyclonal antibody. | |
C15310091 |
H3K27me3S28p Antibody (100 µl) |
ChIP-qPCR DB ELISA IF IP WB | Whole antiserum | |
C15410293 |
H3K27me3S28p Antibody (50 μg) |
ChIP-qPCR DB IF WB | Affinity purified | |
C15410091 |
H3K27me3S28p Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified | |
C15410307 |
H3K36ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified | |
C15410089 |
H3K36me1 Antibody (50 µg/54 µl) |
ChIP-qPCR DB ELISA WB | Affinity purified | |
C15200182 |
H3K36me2 Antibody (50 μg/50 μl) |
ChIP-qPCR DB ELISA WB | Protein A purified monoclonal antibody. | |
C15310127 |
H3K36me2 polyclonal antibody (100 µl) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Whole antiserum from rabbit. | |
C15200183 |
H3K36me3 Antibody (50 μg/50 μl) |
ChIP-qPCR DB ELISA IF WB | Protein A purified | |
C15310058 |
H3K36me3 Antibody (100 µl) |
ChIP-qPCR ChIP-seq DB ELISA WB | Whole antiserum from rabbit containing 0.05% azide. | |
C15410058 |
H3K36me3 Antibody (50 µg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified | |
C15410192 |
H3K36me3 Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified polyclonal antibody. | |
C15210013 |
H3K36me3 Antibody (100 µg) |
ChIP-qPCR ChIP-seq DB WB | Affinity purified polyclonal antibody. | |
C15410295 |
H3K37me1 Antibody (50 μg) |
DB WB | Affinity purified | |
C15410322 |
H3K4ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified polyclonal antibody | |
C15410165 |
H3K4ac Antibody - replaced by the reference C15410322 (50 μg/34 μl) |
ChIP-qPCR DB ELISA IF WB | Affinity purified | |
C15200150 |
H3K4me1 Antibody (50 µg/50 µl) |
ChIP-qPCR ELISA IF WB | Protein A purified | |
C15310037 |
H3K4me1 Antibody (100 µl) |
ChIP-qPCR DB ELISA WB | Whole antiserum | |
C15410037-50 |
H3K4me1 Antibody (50 µg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified polyclonal antibody. | |
C15410194 |
H3K4me1 Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF Peptide array WB | Affinity purified polyclonal antibody. | |
C15210018 |
H3K4me1 Antibody (100 µg) |
ChIP-qPCR ChIP-seq DB IF WB | Affinity purified polyclonal antibody. | |
C15410280 |
H3K4me1T6p Antibody (50 μg) |
DB IF IHC WB | Affinity purified | |
C15200151 |
H3K4me2 Antibody (50 µg/50 µl) |
ChIP-qPCR ELISA IF WB | Protein A purified | |
C15310035 |
H3K4me2 Antibody (100 µl) |
ChIP-qPCR DB ELISA WB | Whole antiserum | |
C15410035 |
H3K4me2 Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified polyclonal antibody. | |
C15200152 |
H3K4me3 Antibody (50 µg) |
ChIP-qPCR ChIP-seq CUT&Tag ELISA IF WB | Protein A purified monoclonal antibody. | |
C15410030 |
H3K4me3 Antibody (50 µg) |
ChIP-qPCR ChIP-seq DB IF WB | Affinity purified polyclonal antibody. | |
C15310003 |
H3K4me3 Antibody (100 µl) |
ChIP-qPCR DB ELISA WB | Whole antiserum | |
C15410003-50 |
H3K4me3 Antibody (50 µg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified polyclonal antibody. | |
C15410281 |
H3K4me3T6p Antibody (50 μg) |
ChIP-qPCR DB IF IHC WB | Affinity purified | |
C15200149 |
H3K4un Antibody (50 µg/50 µl) |
ChIP-qPCR ELISA IF WB | Protein A purified | |
C15410213 |
H3K56ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag ELISA WB | Affinity purified | |
C15410296 |
H3K56me1 Antibody (50 μg) |
ChIP-qPCR DB IF WB | Affinity purified | |
C15410297 |
H3K56me3 Antibody (50 μg) |
DB IF WB | Affinity purified | |
C15410211 |
H3K64me3 Antibody (50 μg) |
ChIP-qPCR DB ELISA WB | Affinity purified | |
C15210006 |
H3K79ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq IF WB | Protein A purified | |
C15310082 |
H3K79me1 Antibody (100 µl) |
ChIP-qPCR DB ELISA WB | Whole antiserum | |
C15410082 |
H3K79me1 Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA WB | Affinity purified | |
C15310051 |
H3K79me2 Antibody (100 µl) |
ChIP-qPCR DB ELISA WB | Whole antiserum | |
C15410051 |
H3K79me2 Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
C15310068 |
H3K79me3 Antibody (100 µl) |
DB ELISA IF WB | Whole antiserum | |
C15410068 |
H3K79me3 Antibody (50 µg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified polyclonal antibody | |
C15410005 |
H3K9/14ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
C15410200 |
H3K9/14ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF Peptide array WB | Affinity purified polyclonal antibody. | |
C15200185-50 |
H3K9ac Antibody (50 μg/50 μl) |
ChIP-qPCR ELISA IF WB | Protein A purified monoclonal antibody | |
C15410004 |
H3K9ac Antibody (50 µg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
C15410177 |
H3K9ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified | |
C15210015 |
H3K9ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq DB WB | Affinity purified polyclonal antibody. | |
C15310102 |
H3K9acS10p Antibody (100 µl) |
ChIP-qPCR DB ELISA IF WB | Whole antiserum | |
C15310065 |
H3K9me1 Antibody (100 µl) |
ChIP-qPCR DB ELISA IF WB | Whole antiserum | |
C15410065 |
H3K9me1 Antibody (50 µg/43 µl) |
ChIP-qPCR DB ELISA WB | Affinity purified | |
C15200154 |
H3K9me2 Antibody (50 μg) |
ChIP-qPCR ELISA IF WB | Protein A purified monoclonal antibody. | |
C15410060 |
H3K9me2 Antibody (50 µg/44 µl) |
ChIP-qPCR DB ELISA IF WB | Affinity purified polyclonal antibody | |
C15210019 |
H3K9me2 Antibody (100 µg/ 100 µl) |
ChIP-qPCR ChIP-seq IF WB | Affinity purified monoclonal antibody | |
C15200146 |
H3K9me3 Antibody (50 μg) |
ChIP-qPCR DB IF WB | Protein A purified | |
C15410013 |
H3K9me3 Antibody (50 µg/39 µl) |
WB | Protein G purified | |
C15100146 |
H3K9me3 Antibody (100 µl) |
ChIP-qPCR DB WB | Concentrated supernatant | |
C15410056 |
H3K9me3 Antibody (50 µg) |
ChIP-qPCR ChIP-seq DB ELISA WB | Affinity purified polyclonal antibody. | |
C15310013 |
H3K9me3 Antibody (100 µl) |
ChIP-qPCR WB | Whole antiserum | |
C15410193 |
H3K9me3 Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified polyclonal antibody. | |
C15210014 |
H3K9me3 Antibody (100 µg) |
ChIP-qPCR ChIP-seq WB | Affinity purified polyclonal antibody. | |
C15200153 |
H3K9me3 Antibody (50 µg/50 µl) |
ChIP-qPCR ELISA IF WB | Protein A purified | |
C15500003-50 |
H3K9me3 recombinant antibody and negative control (50 µg) |
ChIP-qPCR ChIP-seq IF | affinity purified | |
C15310128 |
H3K9me3S10p Antibody (100 µl) |
ChIP-qPCR DB ELISA IF WB | Whole antiserum | |
C15200187 |
H3K9un Antibody (50 μg/25 μl) |
ChIP-qPCR IF WB | Protein A purified | |
C15310135 |
H3pan Antibody (100 µl) |
ChIP-qPCR ELISA WB | Whole antiserum | |
C15410324-50 |
H3pan Antibody (50 μg/16 μl) |
ChIP-qPCR ELISA IF WB | Affinity purified | |
C15200011 |
H3pan Antibody 1B1B2 (50 μg) |
ChIP-qPCR IF WB | Protein A purified monoclonal antibody. | |
C15310092 |
H3R17me2(asym) Antibody (100 µl) |
ChIP-qPCR DB ELISA WB | Whole antiserum | |
C15410289 |
H3R17me2(asym) Antibody (50 μg) |
ChIP-qPCR IF WB | Affinity purified | |
C15410171 |
H3R17me2(asym)K18ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
C15410276 |
H3R2me1 Antibody (50 μg) |
WB | Affinity purified | |
C15410294 |
H3R2me2K4me2 Antibody (50 μg) |
ChIP-qPCR DB IF WB | Affinity purified | |
C15410303 |
H3R2me2sT3p Antibody (50 μg) |
DB IF WB | Affinity purified | |
C15410286 |
H3R8me2(asym) Antibody (50 μg) |
ChIP-qPCR IF IHC WB | Affinity purified | |
C15410287 |
H3R8me2(sym) Antibody (50 μg) |
ChIP-qPCR WB | Affinity purified | |
C15310116 |
H3S10p Antibody (100 µl) |
ChIP-qPCR DB ELISA IF IP WB | Whole antiserum | |
C15410116-50 |
H3S10p Antibody (50 μg/48 μl) |
ChIP-qPCR DB ELISA IF WB | Affinity purified | |
C15410288 |
H3S10pT11p Antibody (50 μg) |
ChIP-qPCR IF IHC WB | Affinity purified | |
C15410309 |
H3T11p Antibody (50 μg) |
ChIP-qPCR DB ELISA IF WB | Affinity purified | |
C15210001 |
H3T3p Antibody (100 μg/100 μl) |
ChIP-qPCR IF WB | Protein A purified | |
C15410279 |
H3T3pK4ac Antibody (50 μg) |
DB IF IHC WB | Affinity purified | |
C15410277 |
H3T3pK4me1 Antibody (50 μg) |
DB IF IHC WB | Affinity purified | |
C15410278 |
H3T3pK4me2 Antibody (50 μg) |
ChIP-qPCR DB IF IHC WB | Affinity purified | |
C15410321 |
H3T45p Antibody (50 μg /34 μl) |
DB ELISA | Affinity purified | |
C15410282 |
H3T6p Antibody (50 μg) |
DB IF IHC WB | Affinity purified | |
C15410283 |
H3T6pK9me1 Antibody (50 μg) |
ChIP-qPCR DB IF IHC WB | Affinity purified | |
C15410284 |
H3T6pK9me2 Antibody (50 μg) |
ChIP-qPCR IF IHC WB | Affinity purified | |
C15410285 |
H3T6pK9me3 Antibody (50 μg) |
ChIP-qPCR DB IF IHC WB | Affinity purified | |
C15410302 |
H4K12ac Antibody (50 μg) |
DB IF IHC WB | Affinity purified | |
C15200218 |
H4K12ac Antibody (50 µg) |
ChIP-qPCR DB WB | Protein A purified | |
C15410331-50 |
H4K12ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF WB | Affinity purified | |
C15200219 |
H4K16ac Antibody (50 µg) |
ChIP-qPCR DB WB | Protein A purified | |
C15410300 |
H4K16ac Antibody (50 μg) |
DB IF WB | Affinity purified | |
C15210008 |
H4K20ac Antibody (100 µg) |
ChIP-qPCR ChIP-seq IF WB | Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide. | |
C15200147 |
H4K20me1 Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB IF WB | Protein A purified | |
C15310034 |
H4K20me1 Antibody (100 µl) |
ChIP-qPCR DB ELISA WB | Whole antiserum | |
C15410034 |
H4K20me1 Antibody (50 μg/25 μl) |
ChIP-qPCR DB ELISA IF WB | Affinity purified | |
C15100147 |
H4K20me1 Antibody (100 µl) |
ChIP-qPCR DB WB | Concentrated supernatant | |
C15200205 |
H4K20me2 Antibody (100 µl) |
ChIP-qPCR IC IF WB | Protein G purified | |
C15200220 |
H4K20me2 Antibody (50 µg) |
ChIP-qPCR DB IF WB | Protein A purified | |
C15410301 |
H4K20me2 Antibody (50 μg) |
ChIP-qPCR IF WB | Affinity purified | |
C15410057 |
H4K20me3 Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified | |
C15410207 |
H4K20me3 Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB ELISA IF Peptide array WB | Affinity purified polyclonal antibody. | |
C15410024 |
H4K5,8,12,16ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq DB IF | Affinity purified polyclonal antibody. | |
C15410021 |
H4K5,8,12ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified | |
C15410025 |
H4K5ac Antibody (50 μg) |
ChIP-qPCR ChIP-seq CUT&Tag DB ELISA IF WB | Affinity purified | |
C15200217 |
H4K8ac Antibody (50 µg) |
ChIP-qPCR DB WB | Protein A purified | |
C15310103 |
H4K8ac Antibody (100 µl) |
ChIP-qPCR DB ELISA WB | Whole antiserum | |
C15410103 |
H4K8ac Antibody (50 μg/41 μl) |
ChIP-qPCR DB ELISA IF WB | Affinity purified | |
C15410156-50 |
H4pan Antibody (50 μg/39 μl) |
ChIP-qPCR ELISA WB | Affinity purified | |
C15410299 |
H4R3me1 Antibody (50 μg) |
ChIP-qPCR DB IC IF WB | Affinity purified | |
C15210022 |
H4R3me1 Antibody (100 µg) |
DB IF WB | Affinity purified monoclonal antibody | |
C15410308 |
H4R3me2(sym) Antibody (50 μg) |
DB ELISA IF WB | Affinity purified | |
C15410298 |
H4S1p Antibody (50 μg) |
ChIP-qPCR DB IF IHC WB | Affinity purified | |
C15210004 |
H4S1p Antibody (100 µg /100 µl) |
ChIP-qPCR IF WB | Protein A purified | |
C15210003 |
macroH2A.1/H2A.2 Antibody (100 µg) |
ChIP-qPCR ChIP-seq IF WB | Protein A purified |