Diagenode

Chromatin function

Chromatin shearing

It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.

Chromatin Shearing Kit Low SDS (for Histone) Chromatin Shearing Kit Low SDS (for TF) Chromatin Shearing Kit High SDS Chromatin Shearing Kit (for Plant)

SDS concentration

< 0.1%

0.2%

1%

0.5%

Nuclei isolation

Yes

Yes

No

Yes

Allows for shearing of... cells/tissue

100 million cells

100 million cells

100 million cells

up to 25 g of tissue

Corresponding to shearing buffers from

iDeal ChIP-seq kit for Histones

iDeal ChIP-seq Kit for Transcription Factors

iDeal ChIP qPCR kit

True MicroChIP kit

Universal Plant ChIP-seq kit

SDS-optimized chromatin shearing

Cat. No.ProductFormatPrice
C01020010 Chromatin shearing optimization kit - Low SDS (iDeal Kit for Histones)
Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. The...
100 million cells $305.00
C01020013 Chromatin shearing optimization kit – Low SDS (iDeal Kit for TFs)
Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. The...
100 million cells $305.00
C01020012 Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit)
Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. The...
100 million cells $305.00
C01020011 Chromatin Shearing Optimization Kit - Medium SDS (HighCell# ChIP kit)
Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. The...
100 million cells $305.00

Plant-optimized chromatin shearing

Cat. No.ProductFormatPrice
C01020014 Chromatin Shearing Optimization Kit (Universal Plant ChIP-seq kit)
The first critical step of a successful ChIP experiment is the best preparation of sheared chromatin. This Chromatin Shearing Optimization Kit is designed to...
12 rxns $305.00

Other shearing optimization kits and buffers

Cat. No.ProductFormatPrice
C01020030 Buffer D, chromatin shearing buffer
Corresponds to the shearing buffer of the Transcription ChIP kit.
70 ml $125.00
C01020021 Shearing ChIP kit - 400
The first critical step of a successful ChIP experiment is the preparation of sheared Chromatin. We therefore suggest the use one of our optimized Shearing C...
400 x 10e6 cells $295.00
C01020022 Shearing Optimization kit
The first critical step of a successful ChIP experiment is the preparation of sheared chromatin. Our Shearing Optimization Kit is perfect for obtai...
40 rxns $615.00

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