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Chromatin studies

Chromatin shearing

The first step of ChIP - chromatin preparation - plays a key role in a succesful ChIP experiment. Each step of chromatin preparation (starting amount of material, cross-linking, cell lysis and chromatin shearing) has to be optimized, leading to a chromatin sample suitable for ChIP. A high quality chromatin sample combines two features: a suitable fragment size range and the availability of epitopes for ChIP.

Diagenode has optimized a range of solutions for successful chromatin preparation. Chromatin EasyShear Kits together with the Bioruptor ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.

Diagenode’s Chromatin EasyShear Kits:

  • Highly optimized solutions for chromatin preparation validated with the Bioruptor
  • Recommended for the optimization of the chromatin shearing of a new cell line/new sample type prior to ChIP
  • Ideal for chromatin preparation prior to ChIP
  • Validated: Kit performance has been validated in ChIP-seq, confirming high quality of chromatin

Chromatin preparation is the first step of a ChIP assay. At Diagenode we offer complete and optimized solutions for ChIP and ChIP-seq, which include all buffers for chromatin preparation. For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs:

Kit choice guide

SAMPLE TYPE SAMPLE INPUT KIT SDS
CONCENTRATION
NUCLEI
ISOLATION
CELLS
< 100,000 Chromatin EasyShear Kit
High SDS
1%
CELLS
> 100,000 Chromatin EasyShear Kit
Ultra Low SDS
< 0.1%
TISSUE
Chromatin EasyShear Kit
Ultra Low SDS
< 0.1%
PLANT TISSUE
Chromatin EasyShear Kit
for Plant
0.5%
FFPE SAMPLES
Chromatin EasyShear Kit
Low SDS
0.2%
CELLS
Chromatin EasyShear Kit
Low SDS
0.2%
TISSUE
FFPE SAMPLES

Guide for optimal chromatin preparation using Chromatin EasyShear Kits Read more

Chromatin EasyShear Kits

Cat. No.ProductFormatPrice
C01020013 Chromatin EasyShear Kit - Low SDS
Previous name of the kit: Chromatin shearing optimization kit – Low SDS (iDeal Kit for TFs) A high quality chromatin preparation is very complex and r...
4 chrom. prep./ 24 rxns $305.00
C01020010 Chromatin EasyShear Kit – Ultra Low SDS
Previous name of the kit: Chromatin shearing optimization kit – Ultra Low SDS A high quality chromatin preparation is very complex and requires a lot ...
10 chrom. prep./60 rxns $305.00
C01020014 Chromatin Shearing Optimization Kit (Universal Plant ChIP-seq kit)
New name of the kit: Chromatin EasyShear Kit for Plant The first critical step of a successful ChIP experiment is the best preparation of sheared chromatin....
12 rxns $305.00
C01020012 Chromatin EasyShear Kit - High SDS
Previous name: Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit) Detergents are essential for successful chromatin shearing. Diagenode of...
100 million cells $305.00

Other shearing optimization kits and buffers

Cat. No.ProductFormatPrice
C01020011 Chromatin Shearing Optimization Kit - Medium SDS (HighCell# ChIP kit)
Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. The...
100 million cells $305.00
C01020022 Shearing Optimization kit
The first critical step of a successful ChIP experiment is the preparation of sheared chromatin. Our Shearing Optimization Kit is perfect for obtai...
40 rxns $615.00

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