Bioruptor® ultrasonicators with adaptive cavitation technology  for highest shearing performance

The Diagenode Bioruptor® is a state-of-the-art sonication system, cited in more than 2,000 publications. The Bioruptor can simultaneously process up to 12 samples with volume flexibility from 0.5 ml to 50 ml to accurately achieve  random and unbiased shearing for different fragment ranges. The closed tube format prevents sample cross-contamination, allowing unmatched reproducibility.  Using a highly-controlled ultrasonic energy with ACT (Adaptive Cavitation Technology), a non-contact, parallel-processing and isothermal technology,  the Bioruptor is ideal for chromatin shearing for chromatin immunoprecipitation (ChIP), genomic DNA shearing for next generation sequencing, RNA shearing, cell and tissue disruption, and much more.

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    The Bioruptor® Pico and Plus utilize a sonication bath-based rotor. The walls of the sonication bath reflect the ultrasound waves in a random but reproducible pattern. The samples in the tube holder are rotated through the ultrasound field to expose each sample to the same level and intensity of energy to ensure shearing consistency. A unique cooling system providing isothermal processing and the gentle ultrasound preserve and retain the integrity of biological samples and ensure high sample recovery.

    Figure: Isothermal and homogeneous sample preparation with the Bioruptor®. Left panels : Precise temperature control to maintain integrity in sample preparation. The single cycle valve and the 4°C water cooler deliver efficient cooling and reliable performance. Finite element analysis software modeling of temperature around the sample (upper panel). Sample temperature measured with Krystal MV64 thermocouple during sonication cycles (30’’/30’’ cycles) showing the perfect isothermal processing (lower panel). Right panels: Powerful and uniform processing of samples down to 5ul (12 samples) to large volumes of 20ml (3 samples). Bioruptor empowers optimal and reproducible chromatin shearing while preserving high protein integrity (upper panel). Bioruptor’s most advanced ultrasound transmitter and water bath design ensure equal energy distribution. Cavitation energy, expressed as % of the maximum level, is measured with PPB Ultrasonic/Megasonic energy meter (lower panel).

    What are the effects of ultrasound on biological samples?

    High powered ultrasound waves can produce gaseous cavitation in liquids. Cavitation is the formation of small bubbles of dissolved gases or vapors due to the alteration of pressure in liquids. These bubbles are capable of resonance vibration and produce vigorous eddying or microstreaming. This mechanical stress has multiple effects on biological samples including; effective chromatin shearing, DNA & RNA shearing, cell lysis and tissue homogenization. When using a probe sonicator, the microstreaming phenomenon is limited to the vicinity of the probe which can generate high amounts of heat and release metal fragments. In contrast, the Bioruptor® sonication bath is equally exposed to ultrasound energy allowing for the dissipation of heat and providing uniform absorption of energy. How does it work? Ultrasonic waves generated by the transducer produce longitudinal vibrations (alternating compression and rarefactions) in the water bath (see figure below). These pressure fluctuations create millions of microscopic bubbles (cavities) caused by the negative pressures generated by the rarefaction stage. As the cavities are exposed to the positive pressures, produced by the compression stage, they oscillate and expand to an unstable size (up to 100 microns diameter). Finally the cavities will implode generating pressure extremes and temperatures at the implosion sites. In addition, shock waves and eddies radiate outwardly from the site of cavity collapse. The localized hot spots that last for microseconds can have temperatures of ~5,000ºC, pressures of ~500 atmospheres as well as heating and cooling rates >109 K/s. The cumulative energy generated by this cavitation phenomenon is extremely high and produces intense shearing forces (Figure 1).

    Figure 1: Presentation of bubble size in function of time during sonication process.

    Bioruptor® is the most popular brand in the Epigenetic and Next-Generation Sequencing communities with more than 2000 citations in peer-reviewed journals. Our instruments provide processing performance and recovery unmatched by any other sample preparation methodology. Bioruptor® high intensity emission of ultrasounds creating cavitation is ideal for Epigenetics studies of chromatin and DNA methylation, Next-Generation Sequencing, protein analysis and much more.

    Diagenode's Bioruptor® simultaneously shears multiple samples in sealed tubes of 0.1 ml to 50 ml capacity quickly and accurately, providing optimal fragment lengths. The Bioruptor® exhibits a laboratory friendly format as it is easily programmable and capable of processing multiple sample tubes, with parallel processing of 3 - 12 samples. The instrument is able to sonicate different sample volumes to achieve different fragment size ranges. The closed tube format prevents the sample from cross-contamination and aerosol formation.

Applications Bioruptor® Pico Bioruptor® Plus
NGS DNA shearing
Chromatin shearing
NGS RNA shearing
DNA extraction from FFPE
Cell and tissue disruption for protein extraction (for mass spectrometry or other applications)
Cell and tissue disruption for RNA extraction
Bacterial cell lysis
Compound solubilization
Liposome size reduction
Technical specification
Temperature control
Low noise level
Small footprint
Fast processing*
Shearing volume 5 µl -2 ml 50 µl -20 ml
Throughput Up to 16 samples per run Up to 12 samples per run

 Excellent  Good  Less suitable
* Time required to simultaneously shear 12 genomic DNA (10ng/ul, 100ul samples) to 200bp with Bioruptor® Pico = 13min and Bioruptor® Plus = 30min.



The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system optimized for shearing micro-...

  • Temperature-controlled
  • Easy to use
  • All-in-one solution
  • Processing of 6-12 samples
  • Sample size 5 µl – 2 ml
  • No soundproof box
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The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (wate...

  • Temperature-controlled
  • Easy to use
  • Processing of 3-12 samples
  • Sample size 100 µl – 20 ml
  • Advanced timing control

Bioruptor® Pico accessories

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    0.1 ml Bioruptor® Pico Microtubes
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    0.1 ml tube holder & tube adaptors for Biorupto...
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    0.5/0.65 ml tube holder for Bioruptor® Stan...
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    0.65 ml Bioruptor® Pico Microtubes
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    1.5 ml Bioruptor® Pico Microtubes with Caps
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    1.5 ml tube holder for Bioruptor® Pico
  • 15 ml tubes
    15 ml Bioruptor® Pico Tubes
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    View more products from the Bioruptor® Pico accessories product category.

Bioruptor® Plus (and Standard) accessories

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    0.5 ml Bioruptor® Plus Microtubes
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    0.5/0.65 ml tube holder for Bioruptor® Stan...
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    1.5 ml Bioruptor® Plus TPX microtubes
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    1.5 ml Bioruptor® Plus TPX microtubes
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    1.5 ml tube holder for Bioruptor® Standard ...
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    10 ml Bioruptor® Plus tubes
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    10 ml tube holder for Bioruptor® Standard & Plus
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    View more products from the Bioruptor® Plus (and Standard) accessories product category.

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