Diagenode

Next Generation Sequencing

RNA extraction

Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption and homogenization of tissues and cultured cells are required. Diagenode's Bioruptor® Plus uses state-of-the-art ultrasound ACT (Adaptive Cavitation Technology) combined with unique devices such as beads or tubes acting as sonication « enhancers » or dedicated reagents (such as our RNA extraction reagent) to efficiently disrupt tissues and cultured cells in just one step to deliver high quality RNA extraction.
Efficient extraction of pure RNA with high RIN. 
Total RNA profiles from mouse brain. Tissue was disrupted with Bioruptor® Plus as described in the protocol and analyzed on BioAnalyzer (Agilent). Note that small RNAs are present in all profiles indicating that the RNA is largely intact.

Recommended products for RNA extraction

  • some alt
    B01020001
    Bioruptor® Plus sonication device
  • some alt
    C30010010-300
    1.5 ml Bioruptor® Plus TPX microtubes
  • some alt
    C30010010-50
    1.5 ml Bioruptor® Plus TPX microtubes
  • some alt
    B01200011
    1.5 ml tube holder for Bioruptor® Standard ...
  • some alt
    C30010009
    15 ml Bioruptor® Plus TPX tubes
  • some alt
    B01200016
    15 ml sonication accessories for Bioruptor®...
  • Kit icon
    C20000010
    RNA Extraction kit for Bioruptor Plus

Related protocols

RNA extraction from tissue using Bioruptor® (Standard/Plus) and RNA extraction kit
Isolation of intact RNA is essential for many techniques used in gene expression analysis. Effici...
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Events

  • Genome instability: when RNA meets chromatin
    Roscoff
    Sep 16-Jun 20, 2019
  • Japanese Biochemical Society
    Yokohama
    Sep 18-Sep 20, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    Atlanta, Georgia
    Sep 19, 2019
  • The 2019 PacBio Long-Read Revolution: Highly Accurate and Affordable SMRT Sequencing
    RTP, North Carolina
    Sep 20, 2019
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