Diagenode

ChIPmentation for high-quality and fast ChIP-seq library preparation

Diagenode now offers a unique system that incorporates automation and a ligation-free protocol for chromatin immunoprecipitation coupled with library preparation for high-throughput sequencing (ChIP-seq), ChIPmentation. ChIPmentation is based on tagmentation which enables the integration of the library preparation during the ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates a much easier and shorter protocol with high efficiency for low input samples. The new ChIPmentation system is available only on the IP-Star Automation System ensuring the highest reproducibility for challenging samples of limited quantities.

Benefits of the ChIPmentation system for optimal ChIP-seq

  • Automate on the IP-Star® to support standardization and reproducibility
  • Save one full day from standard protocols in generating ChIP-seq libraries
  • Enjoy an easier protocol
  • Benefit from the elimination of sequencing adaptor dimers
  • Ensure high quality data on low cell numbers and rare cell types
Diagenode-ChIPmentation

In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.

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Products

Cat. No.ProductFormatPrice
C01011000 Auto ChIPmentation for Histones
This product must be used with the IP-Star Compact Automated System. Difficult and challenging histone ChIP-seq is now solved. Diagenode’s latest tech...
24 rxns $2,320.00

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