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Chromatin studies

ChIPmentation™

ChIPmentation for high-quality and fast
ChIP-seq library preparation

Diagenode’s ChIPmentation technology, based on tagmentation, enables the integration of library preparation during ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates an easier and shorter protocol.

Different protocols have been optimized bringing the solution for different needs:

  • Low cell number – The kit µChIPmentation for Histones enables to use as little as 10.000 cells per reaction New!
  • Standard cell number – ChIPmentation kit for Histones - two complete protocols have been optimized: a manual procedure and an automated protocol on the IP-Star Compact Automated System.
  • Module for library prep using tagmentationTag Kit for ChIPmentation can be used with any ChIP protocol

Benefits of the ChIPmentation system for optimal ChIP-seq

  • Ensure high quality sequencing data
  • Perform ChIP-seq on low input samples
  • Enjoy an easy and fast protocol
  • Benefit from the elimination of sequencing adaptor dimers
  • Automate on the IP-Star® to support standardization and save time
  • ChIPmentation workflow - Read more
    Diagenode-ChIPmentation
    In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.

Complete kits:

Modules:

  • TAG Kit for ChIPmentation
    The TAG Kit for ChIPmentation has been developed for researchers willing to perform ChIPmentation using their own validated ChIP protocol.
    Read more
  • 24 SI for ChIPmentation
    The 24 SI for ChIPmentation includes 24 single indexes compliting the TAG Kit for ChIPmentation.
    Read more
  • 24 SI for Tagmented libraries
    The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. This set of indexes is compatible with any tagmentation-based library preparation protocols, such as ChIPmentation, ATAC-seq or CUT&Tag technologies.
    Read more

Separately available products:

  • Tagmentase (Tn5 transposase)
    Diagenode Tagmentase is a hyperactive Tn5 transposase with the ability to cut DNA and insert sequences of interest into any target DNA in one step. This enzyme is not loaded with DNA oligos.
    Read more
  • Tagmentase Dilution Buffer
    Diagenode Tagmentase Dilution Buffer is recommended for dilution of Tagmentase (Cat. No. C01070010) before or after transposome assembly.
    Read more

Products

Cat. No.ProductFormatPrice
C01011032 24 SI for Tagmented libraries
The 24 SI for tagmented libraries includes 24 single primer indexes for multiplexing up to 24 samples. The primer indexes of the 24 SI Tagmented libraries ar...
24 SI $170.00
C01011011 µChIPmentation Kit for Histones
The Diagenode µChIPmentation Kit for Histones is optimized to perform ChIP-seq on as little as 10.000 cells from cell fixation to purified libraries. T...
24 rxns $2,150.00
C01011031 24 SI for ChIPmentation
Contact us The 24 SI for ChIPmentation includes 24 single primer indexes completing the TAG Kit for ChIPmentation. This set of indexes allows for...
24 indexes $170.00
C01011000 Auto ChIPmentation Kit for Histones
Contact us This product must be used with the IP-Star Compact Automated System. Diagenode’s latest technology for histone ChIP-seq, ...
24 rxns $2,320.00
C01011010 ChIPmentation Kit for Histones
Contact us Difficult and challenging histone ChIP-seq is now solved. Diagenode’s latest technology for histone ChIP-seq, ChIPmentation ...
24 rxns $2,320.00
C01011030 TAG Kit for ChIPmentation
Contact us Tagmentation is a reaction where an enzyme (a transposase) cleaves DNA and incorporates sequencing adaptors at the ends of the DNA fra...
24 rxns $950.00

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