ChIPmentation for high-quality and fast ChIP-seq library preparation
Diagenode now offers a unique system that incorporates automation and a ligation-free protocol for chromatin immunoprecipitation coupled with library preparation for high-throughput sequencing (ChIP-seq), ChIPmentation. ChIPmentation is based on tagmentation which enables the integration of the library preparation during the ChIP itself using transposase and sequencing-compatible adaptors. Unlike standard library preparation techniques that require multi-step ligation, ChIPmentation incorporates a much easier and shorter protocol with high efficiency for low input samples. The new ChIPmentation system is available only on the IP-Star Automation System ensuring the highest reproducibility for challenging samples of limited quantities.
Benefits of the ChIPmentation system for optimal ChIP-seq
- Automate on the IP-Star® to support standardization and reproducibility
- Save one full day from standard protocols in generating ChIP-seq libraries
- Enjoy an easier protocol
- Benefit from the elimination of sequencing adaptor dimers
- Ensure high quality data on low cell numbers and rare cell types
In traditional ChIP-seq library preparation, the ligation of the adaptors is performed after chromatin immunoprecipitation, in three steps. In the Diagenode ChIPmentation workflow, not only are the adaptors directly incorporated during ChIP, but also the main steps are automated on the Diagenode IP-Star®. The combination of direct adaptor incorporation and automation allows for higher sensitivity from low cell numbers and reproducibility of results.
ChIPmentation - Highest reliability for even the lowest inputs
The new Auto-ChIPmentation technology has been tested on histone marks and compared to available datasets from the ENCODE project (Figure 1). ChIPmentation generated high quality data with low background. In addition, more than 99% of the top 40% peaks obtained with auto-ChIPmentation overlap with ENCODE datasets, which shows that ChIP-seq data obtained with ChIPmentation are highly reliable.
B. Match of the Top40 peaks of Auto-ChIPmentation
B. Match of the Top40 peaks of ChIPmentation from 10.000 cells
C. Match of the Top40 peaks of ChIPmentation from 5.000 cells
A. Auto ChIPmentation on 10.000 cells
B. Auto ChIPmentation on 5.000 cells
The Auto-ChIPmentation workflow is easy and fast, producing high-quality ChIP-seq data even on low starting amounts.
Kit for Auto ChIPmentation - coming soon!
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