Chromatin immunoprecipitation (ChIP) determines the location of DNA binding sites on the genome for a protein of interest, giving insights into gene expression regulation. ChIP involves the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognize a protein or protein modification are used to determine the relative abundance of that antigen at a specific locus or loci. ChIP can be used to compare enrichment of proteins, map protein modifications, or quantify a protein modification during a time course.
The ChIP workflow
Products for chromatin study
Efficient solution for protein-protein fixation. Read more
Perfectly sheared chromatin is critical for ChIP success.
Read more about solutions for successful chromatin preparation.
Read more about Bioruptor sonication.
Immunoprecipitation solutions for histone and transcription factor ChIP-qPCR and ChIP-seq for low inputs, plants, and animals including automated solutions. Read more
ChIPmentation, an exclusive technology, is an end-to-end ChIP-seq solution for low and difficult inputs. Read more
ChIP and ChIP-seq antibodies
ChIP-grade antibodies are essential for success. Learn more
Highly specific primer pairs for the amplification of the specific genomic regions. Read more
An alternative to ChIP-seq that combines antibody-targeted controlled cleavage by a protein A-Tn5 fusion with NGS to identify the binding sites of DNA-associated proteins. Read more
Read more about solutions for DNA purification
Library preparation for ChIP-seq
Optimized solutions for the library preparation from low DNA input. Read more
ChIP and ChIP-seq automation
Reproducibility, optimization simplicity, no variability. Learn more
We offer complete ChIP kits or individual kit components from antibodies, buffers, beads, chromatin shearing, and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.
Publications related to ChIP