Chromatin studies

Chromatin immunoprecipitation (ChIP) is used to map the location of DNA binding sites on the genome for specific proteins, giving invaluable insights into gene expression regulation.  ChIP involves the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognize a specific protein or protein modification are used to determine the relative abundance of that antigen at a specific locus or loci.. ChIP is most often analyzed using qPCR (ChIP-qPCR) or sequencing (ChIP-seq). Read more

The ChIP workflow

Step by step workflow

Optimal solution from Diagenode

1. Crosslink to bind proteins to DNA

2. Shear DNA

3. Immunoprecipitate with specific antibody

4. Reverse crosslinks and purify

  • Diagenode's ChIP kits (contain optimal purification modules)

5. ChIP-qPCR or ChIP-seq library preparation

  • ChIP-seq:
  • ChIP qPCR

Products for chromatin study

  Chromatin shearing
Perfectly sheared chromatin is critical for ChIP success. 
Read more about reagent optimization. 
Read more about Bioruptor sonication.

  Chromatin immunoprecipitation
Immunoprecipitation solutions for histone and transcription factor ChIP-qPCR and ChIP-seq for low inputs, plants, and animals including automated solutionsRead more

ChIPmentation, an exclusive technology, is an end-to-end ChIP-seq solution for low and difficult inputs. Read more

  ChIP and ChIP-seq antibodies
ChIP-grade antibodies are essential for success. Learn more

  ChIP and ChIP-seq automation
Reproducibility, optimization simplicity, no variability. Learn more

  Chromatin assembly products
Study the nucleosome environment. 
Learn more

Chromatin resources


Application notes

Publications related to ChIP


Chromatin shearing

Chromatin immunoprecipitation


Cat. No.ProductFormatPrice
C01011000 Auto ChIPmentation Kit for Histones
Contact us This product must be used with the IP-Star Compact Automated System. Difficult and challenging histone ChIP-seq is now solved. ...
24 rxns $2,320.00
C01011010 ChIPmentation Kit for Histones
Contact us Difficult and challenging histone ChIP-seq is now solved. Diagenode’s latest technology for histone ChIP-seq, ChIPmentation ...
24 rxns $2,320.00

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