Diagenode

ChIP Cross-link Gold

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Catalog Number
Format
Price
C01019027
600 µl
$125.00

Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.However, formaldehyde is usually not effective to cross-link proteins that are not directly bound to the DNA.So, for higher order and/or dynamic interactions, other cross-linkers should be considered for efficient protein-protein stabilization such as the Diagenode ChIP cross-link Gold. This reagent is to use in combination with formaldehyde.

  • Protocols
    Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor® Pico
    Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in c...
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  • Applications
    ChIP-qPCR
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
    ChIP-seq
    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomic researches, namely to investigate protein-DNA interaction on a ... Read more
    Chromatin shearing
    The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, Diagenode's Bio... Read more
  • Documents
    ChIP cross-link Gold MANUAL
    The first step of a ChIP assay is the cross-linking in order to fix the cells. Cross-linkin...
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  • Publications

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