Diagenode

ChIP Cross-link Gold

Catalog Number
Format
Price
C01019027
600 µl
$130.00

Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective in cross-linking proteins that are not directly bound to the DNA. For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. For such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is used in combination with formaldehyde is an excellent choice for such higher order protein interactions. 

  •  Protocols
    Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor® Pico
    Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in c...
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  •  Applications
    ChIP-qPCR
    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory region... Read more
    ChIP-seq
    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on ... Read more
    Chromatin shearing
    The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, Diagenode's Bio... Read more
    Chromatin Analysis
    Chromatin immunoprecipitation (ChIP) is a method used to determine the location of genome binding sites for a specific protein of interest, giving invaluable insights into the regulation of gene expression. ChIP involved the selective enrichme... Read more
  •  Documents
    ChIP cross-link Gold MANUAL
    The first step of a ChIP assay is the cross-linking in order to fix the cells. Cross-linkin...
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  •  Publications

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    SMC Progressively Aligns Chromosomal Arms in Caulobacter crescentus but Is Antagonized by Convergent Transcription
    Tran N.T. et al.
    The structural maintenance of chromosomes (SMC) complex plays an important role in chromosome organization and segregation in most living organisms. In Caulobacter crescentus, SMC is required to align the left and the right arms of the chromosome that run in parallel down the long axis of the cell. However, the...

    EPOP Functionally Links Elongin and Polycomb in Pluripotent Stem Cells
    Beringer M. et al.
    The cellular plasticity of pluripotent stem cells is thought to be sustained by genomic regions that display both active and repressive chromatin properties. These regions exhibit low levels of gene expression, yet the mechanisms controlling these levels remain unknown. Here, we describe Elongin BC as a binding fact...

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