Diagenode

ChIP Cross-link Gold

Catalog Number
Format
Price
C01019027
600 µl
$130.00

Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links. However, formaldehyde is usually not effective in cross-linking proteins that are not directly bound to the DNA. For example, inducible transcription factors or cofactors interact with DNA through protein-protein interactions, and these are not well preserved with formaldehyde. For such higher order and/or dynamic interactions such as this, other cross-linkers should be considered for efficient protein-protein stabilization. Diagenode's ChIP cross-link Gold which is used in combination with formaldehyde is an excellent choice for such higher order protein interactions. 

  •  Protocols
    Chromatin shearing - Guide for successful chromatin preparation using the Bioruptor® Pico
    Chromatin immunoprecipitation (ChIP) is a common tool used to study protein-DNA interactions in c...
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  •  Documents
    ChIP cross-link Gold MANUAL
    The first step of a ChIP assay is the cross-linking in order to fix the cells. Cross-linkin...
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  •  Publications

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    P-TEFb Activation by RBM7 Shapes a Pro-survival Transcriptional Response to Genotoxic Stress.
    Bugai A, Quaresma AJC, Friedel CC, Lenasi T, Düster R, Sibley CR, Fujinaga K, Kukanja P, Hennig T, Blasius M, Geyer M, Ule J, Dölken L, Barborič M
    DNA damage response (DDR) involves dramatic transcriptional alterations, the mechanisms of which remain ill defined. Here, we show that following genotoxic stress, the RNA-binding motif protein 7 (RBM7) stimulates RNA polymerase II (Pol II) transcription and promotes cell viability by activating the positive transcr...

    The BRG1/SOX9 axis is critical for acinar cell-derived pancreatic tumorigenesis.
    Tsuda M, Fukuda A, Roy N, Hiramatsu Y, Leonhardt L, Kakiuchi N, Hoyer K, Ogawa S, Goto N, Ikuta K, Kimura Y, Matsumoto Y, Takada Y, Yoshioka T, Maruno T, Yamaga Y, Kim GE, Akiyama H, Ogawa S, Wright CV, Saur D, Takaori K, Uemoto S, Hebrok M, Chiba T, Seno
    Chromatin remodeler Brahma related gene 1 (BRG1) is silenced in approximately 10% of human pancreatic ductal adenocarcinomas (PDAs). We previously showed that BRG1 inhibits the formation of intraductal pancreatic mucinous neoplasm (IPMN) and that IPMN-derived PDA originated from ductal cells. However, the role of BR...

    A new metabolic gene signature in prostate cancer regulated by JMJD3 and EZH2.
    Daures M, Idrissou M, Judes G, Rifaï K, Penault-Llorca F, Bignon YJ, Guy L, Bernard-Gallon D
    Histone methylation is essential for gene expression control. Trimethylated lysine 27 of histone 3 (H3K27me3) is controlled by the balance between the activities of JMJD3 demethylase and EZH2 methyltransferase. This epigenetic mark has been shown to be deregulated in prostate cancer, and evidence shows H3K27me3 enri...

    SMC Progressively Aligns Chromosomal Arms in Caulobacter crescentus but Is Antagonized by Convergent Transcription
    Tran N.T. et al.
    The structural maintenance of chromosomes (SMC) complex plays an important role in chromosome organization and segregation in most living organisms. In Caulobacter crescentus, SMC is required to align the left and the right arms of the chromosome that run in parallel down the long axis of the cell. However, the...

    EPOP Functionally Links Elongin and Polycomb in Pluripotent Stem Cells
    Beringer M. et al.
    The cellular plasticity of pluripotent stem cells is thought to be sustained by genomic regions that display both active and repressive chromatin properties. These regions exhibit low levels of gene expression, yet the mechanisms controlling these levels remain unknown. Here, we describe Elongin BC as a binding fact...

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