CATS relies on polynucleotide tailing for capturing the 3’-ends of nucleic acids and the template switching ability of MMLV-RT to capture the 5’-ends for cDNA synthesis.
The Diagenode kits generate Illumina compatible ready-to-sequence libraries from RNA inputs as low as 10 pg in just few hours. Libraries retain strand specificity of origin. Unlike competing solutions, our CATS RNA-seq Kits exhibit higher library efficiency versus ligation-based methods. The CATS method allows for accurate identification of a greater number of non-coding RNAs, providing higher complexity from better template capture and minimal bias due to lower amplification requirements.