Diagenode

NGS Library preparation

D-Plex RNA library preparation

Total RNA-seq library prep kit for Illumina

D-Plex technology is the latest innovation in RNA-sequencing. This ultrasensitive, ligation-free method utilizes the capture and amplification by tailing and switching for library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs. This technology offers a new RNA-sequencing solution, optimized and specifically formatted for both Illumina and MGI sequencing platforms.

Using D-Plex technology is optimal for the study of non-coding RNAs that regulate gene expression at the transcriptional and post-transcriptional levels. The library preparation enables diverse and novel transcript detection, ensuring realistic representation of the transcriptome with minimized bias. The unique molecular identifier (UMI) sequence added in D-Plex Indexes additionally enables the accurate bioinformatic identification and removal of PCR duplicates from amplified libraries.

Our new D-Plex RNA-seq kits provide streamlined workflows with easy-to-follow protocols. The library preparation takes place in a single tube, increasing the efficiency tremendously. This ensures high technical reliability and reproducibility. Our D-Plex RNA-seq kits have been extensively validated for clinically relevant samples such as liquid biopsies or FFPE samples. The D-Plex technology is optimal for epigenetic biomarker discovery.

Why the new D-Plex technology benefits RNA research?

  • Ultrasensitive, ligation-free method for RNA-seq library preparation
  • Ultra-low input capability, down to 10 pg for small RNAs and 100 pg for total RNAs
  • High library complexity for novel and diverse transcript detection
  • Improved quantification accuracy through the use of UMIs
  • Optimal performance on clinical samples, such as liquid biopsies and FFPE samples
  • Easy to use with minimal hands-on time: one day, one tube protocol
  • Compatible for both Illumina and MGI sequencing platforms

Read more about D-Plex technology.

Illumina sequencing platform

Cat. No.ProductFormatPrice
C05030033 D-Plex mRNA-seq Kit for Illumina
Product Features An innovative technology with template switching and UMIs Ultra-sensitive methodology with low input capability down to 10 ng High ef...
24 rxns $1,390.00
C05030010 D-Plex Single Indexes for Illumina - Set A
D-Plex Single Indexes Module - Set A includes PCR primers with 24 barcodes (i7 index) for library multiplexing with the D-Plex Small...
24 rxns $300.00
C05030011 D-Plex Single Indexes for Illumina - Set B
D-Plex Single Indexes Module - Set B includes PCR primers with 24 barcodes (i7 index) for library multiplexing with the D-Plex ...
24 rxns $300.00
C05030001 D-Plex Small RNA-seq Kit for Illumina
Product Features Ultra-low input capability, down to 10 pg for small RNAs or circulating RNAs Higher library complexity providing novel and diverse tra...
24 rxns $1,320.00
C05030031 D-Plex Total RNA-seq Kit for Illumina
Product Features An innovative technology with template switching and UMIs Ultra-low input capability, down to 50 pg for total RNAs Capture the widest...
24 rxns $1,320.00
C05030021 D-Plex Unique Dual Indexes for Illumina - Set A
D-Plex Unique Dual Indexes Module - Set A includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multiplexi...
24 rxns $320.00
C05030022 D-Plex Unique Dual Indexes for Illumina - Set B
D-Plex Unique Dual Indexes Module - Set B includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multiplexi...
24 rxns $320.00
View more products from the Illumina sequencing platform product category...

MGI sequencing platform

Cat. No.ProductFormatPrice
C05030060 D-Plex DNBSEQ Barcodes for MGI Sequencing - Set A
D-Plex DNBSEQ Barcodes - Set A includes primers with 24 barcodes to enable multiplexing with the D-Plex Small RNA DNBSEQTM Kit. ...
24 rxns $300.00
C05030061 D-Plex DNBSEQ Barcodes for MGI Sequencing - Set B
D-Plex DNBSEQ Barcodes - Set B includes primers with 24 barcodes to enable multiplexing with the D-Plex Small RNA...
24 rxns $300.00
C05030051 D-Plex Small RNA DNBSEQ™ Kit for MGI Sequencing
Download the manual D-Plex Small RNA DNBSEQ™ Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the D-...
24 rxns $1,320.00

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