Diagenode

>>>   Click for Diagenode’s approach to COVID-19

NGS Library preparation

Library preparation for RNA sequencing

D-Plex Small RNA-seq library prep kit with UMI for Illumina

D-Plex technology is the latest innovation in RNA-sequencing. This ultrasensitive, ligation-free method utilizes the capture and amplification by tailing and switching for library preparation from ultra-low input amounts, down to 10 pg for small RNAs and 100 pg for total RNAs. This technology offers a new RNA-sequencing solution, optimized and specifically formatted for both Illumina and MGI sequencing platforms.

Using D-Plex technology is optimal for the study of non-coding RNAs that regulate gene expression at the transcriptional and post-transcriptional levels. The library preparation enables diverse and novel transcript detection, ensuring realistic representation of the transcriptome with minimized bias. The unique molecular identifier (UMI) sequence added in D-Plex Indexes additionally enables the accurate bioinformatic identification and removal of PCR duplicates from amplified libraries.

Our new D-Plex RNA-seq kits provide streamlined workflows with easy-to-follow protocols. The library preparation takes place in a single tube, increasing the efficiency tremendously. This ensures high technical reliability and reproducibility. Our D-Plex RNA-seq kits have been extensively validated for clinically relevant samples such as liquid biopsies or FFPE samples. The D-Plex technology is exceedingly suitable for epigenetic biomarker discovery.

Why the new D-Plex technology benefits RNA research?

  • Ultrasensitive, ligation-free method for RNA-seq library preparation
  • Ultra-low input capability, down to 10 pg for small RNAs and 100 pg for total RNAs
  • High library complexity ensuring novel and diverse transcript detection
  • Improved quantification accuracy through the use of UMI
  • Optimal performance on clinical samples, such as liquid biopsies
  • Easy to use with minimal hands-on time: one day, one tube protocol
  • Compatible for both Illumina and MGI sequencing platforms

Read more about D-Plex technology.

Illumina sequencing platform

Cat. No.ProductFormatPrice
C05030010 D-Plex Single Indexes for Illumina - Set A
D-Plex Single Indexes Module - Set A includes PCR primers with 24 barcodes (i7 index) for library multiplexing with the D-Plex Small...
24 rxns $295.00
C05030011 D-Plex Single Indexes for Illumina - Set B
D-Plex Single Indexes Module - Set B includes PCR primers with 24 barcodes (i7 index) for library multiplexing with the D-Plex ...
24 rxns $295.00
C05030001 D-Plex Small RNA-seq Kit for Illumina
Download the manual Note: The D-Plex Small RNA-seq Kit manual describes now the protocol for both UDI and SI library constructions. D-Plex Small...
24 rxns $1,295.00
C05030021 D-Plex Unique Dual Indexes for Illumina - Set A
D-Plex Unique Dual Indexes Module - Set A includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multiplexi...
24 rxns $315.00
C05030022 D-Plex Unique Dual Indexes for Illumina - Set B
D-Plex Unique Dual Indexes Module - Set B includes primer pairs with 24 unique dual barcodes (unique i5 and i7 indexes) for library multiplexi...
24 rxns $315.00
C05010043 CATS mRNA-seq Kit (with polyA selection) v2 x24
Diagenode’s CATS mRNA-seq Kit (with polyA selection) utilizes the innovative “Capture and Amplification by Tailing and Switching” (CATS), a...
24 rxns $1,479.00
C05010041 CATS RNA-seq Kit v2 x24
Diagenode’s CATS RNA-seq Kit utilizes the innovative “Capture and Amplification by Tailing and Switching” (CATS), a ligation-free method to...
24 rxns $1,438.00
View more products from the Illumina sequencing platform product category...

MGI sequencing platform

Cat. No.ProductFormatPrice
C05030060 D-Plex DNBSEQ Barcodes for MGI Sequencing - Set A
D-Plex DNBSEQ Barcodes - Set A includes primers with 24 barcodes to enable multiplexing with the D-Plex Small RNA DNBSEQTM Kit. ...
24 rxns $295.00
C05030061 D-Plex DNBSEQ Barcodes for MGI Sequencing - Set B
D-Plex DNBSEQ Barcodes - Set B includes primers with 24 barcodes to enable multiplexing with the D-Plex Small RNA...
24 rxns $295.00
C05030051 D-Plex Small RNA DNBSEQ™ Kit for MGI Sequencing
Download the manual D-Plex Small RNA DNBSEQ™ Kit is a tool designed for the study of the small non-coding transcriptome. The kit is using the D-...
24 rxns $1,295.00

Events

 See all events

News

 See all news


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics