The template-switch protocol is much more efficient for RNA-seq library creation than protocols that use ligase by incorporating sequencing adaptors during cDNA synthesis in a single reaction tube. D-Plex thus allows for optimal sequencing of sensitive, degraded, rare, and low input RNA samples. The CATS and D-Plex RNA-seq kits for library preparation are ideal for studying extracellular cell-free RNA (cfRNA) that may potentially represent cancer biomarkers, and are detected as serum or plasma-derived RNA, FFPE-derived RNA, and exosomal RNAs.
Additionally, template switch-based RNA sequencing is optimal for the study of non-coding RNA (ncRNA) that regulate gene expression at the transcriptional and post-transcriptional level including miRNA and long non-coding RNA ( lncRNA).