Diagenode

DNA methylation

Methylated DNA Immunoprecipitation

The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.

How it works

In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.

Overview


    qPCR

    NGS

ADVANTAGES

  • Unaffected DNA
  • High enrichment yield
  • Robust & reproducible techniques
  • NGS compatible

产品

目录号产品格式价格
C02010021 MagMeDIP qPCR Kit
Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo...
48 rxns (IP) $670.00
C02010014 Auto MagMeDIP qPCR Kit
Perform MeDIP (Methylated DNA Immunoprecipitation) on the SX-8G IP-Star® Automated System followed by qPCR to estimate DNA methy...
48 rxns $670.00
C02040012 DNA Methylation control package
The DNA Methylation control package includes unmethylated and in vitro methylated DNA together with specific primer sets for assessing the efficiency of your...
40 rxns $275.00
C02040019 DNA methylation control package V2
The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls that can be added directly to the DNA sample of interes...
48 rxns $250.00

活动

  • 64th ASH Annual Meeting and Exposition 2022
    New Orleans, Louisiana, USA
    Dec 10-Dec 13, 2022
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