DNA methylation

Methylated DNA Immunoprecipitation

The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.

How it works

In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.

Overview

Collect and lyse cells

Extract and shear DNA

Prepare library for MeDIP-seq

Immunoprecipitate, wash and isolate DNA

qPCR

NGS

ADVANTAGES

Unaffected DNA
High enrichment yield
Robust & reproducible techniques
NGS compatible

产品

目录号	产品	格式	价格
C02010021	MagMeDIP qPCR Kit Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo...	48 rxns	$658.00
C02010040	MagMeDIP-seq Package *Use the MAGPACK02 promo code in your online order or by clicking on the banner. Sensitive tumour detection and classification using plasma cel...	10 rxns	$1,392.00
C02010041	MagMeDIP-seq Pacakge V2 (Coming soon)Perform MeDIP-seq experiments, i.e. methylated DNA immunoprecipitation followed by next generation sequencing, to obtain reg...	10 rxns		Get a quote You are about to request a quote for MagMeDIP-seq Pacakge V2. Fill out the form below and we will be in touch with you very soon. All * fields are mandatory Contact Information First name ^* Last name ^* Company ^* Phone number City Country ^* State Email ^* Email verification^* Comment ×
C02010014	Auto MagMeDIP qPCR Kit Perform MeDIP (Methylated DNA Immunoprecipitation) on the SX-8G IP-Star® Automated System followed by qPCR to estimate DNA methy...	48 rxns	$658.00
C02040012	DNA Methylation control package The DNA Methylation control package includes unmethylated and in vitro methylated DNA together with specific primer sets for assessing the efficiency of your...	40 rxns	$270.00
C02040019	DNA methylation control package V2 The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls that can be added directly to the DNA sample of interes...	48 rxns	$270.00

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Methylated DNA Immunoprecipitation

How it works

Overview

ADVANTAGES

产品

活动

新闻