The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.
In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.
目录号 | 产品 | 格式 | 价格 | |
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C02010021 |
MagMeDIP qPCR Kit Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo... |
48 rxns (IP) | $670.00 | |
C02010041 |
MagMeDIP-seq Pacakge V2 (Coming soon)Perform MeDIP-seq experiments, i.e. methylated DNA immunoprecipitation followed by next generation sequencing, to obtain reg... |
10 rxns | $1,850.00 | |
C02010014 |
Auto MagMeDIP qPCR Kit Perform MeDIP (Methylated DNA Immunoprecipitation) on the SX-8G IP-Star® Automated System followed by qPCR to estimate DNA methy... |
48 rxns | $670.00 | |
C02040012 |
DNA Methylation control package The DNA Methylation control package includes unmethylated and in vitro methylated DNA together with specific primer sets for assessing the efficiency of your... |
40 rxns | $275.00 | |
C02040019 |
DNA methylation control package V2 The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls that can be added directly to the DNA sample of interes... |
48 rxns | $250.00 | |
C02010040 |
MagMeDIP-seq Package *Use the MAGPACK02 promo code in your online order or by clicking on the banner. Sensitive tumour detection and classification using plasma cel... |
10 rxns |