Diagenode

DNA methylation

Bisulfite conversion


Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level. Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.

How it works

Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figures).

ADVANTAGES

  • Single nucleotide resolution
  • Gene-specific and genome-wide analyses
  • NGS compatible

Downstream analysis techniques

产品

目录号产品格式价格
C02030030 Premium Bisulfite kit
Make your Bisulfite conversion now in only 90 minutes ! Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion r...
50 rxns $240.00
C02030032 Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit
Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level withou...
24 rxns
C02030033 Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit
Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level withou...
96 rxns
C02030036 Premium RRBS kit V2
(Coming soon) Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, targeted solution to perform genome-scale DNA methylation analysis...
24 rxns $1,590.00
C02030037 Premium RRBS kit V2 x96
RRBS for low DNA amounts and accurate analysis

Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, focused solution to perform genome-scale DNA methylation analysis at the s...
96 rxns $3,745.00
C02030031 Auto Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
40 rxns $240.00
C02030035 Bisulfite conversion reagent for RRBS
This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S...
8 rxns $120.00
C02040019 DNA methylation control package V2
The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls that can be added directly to the DNA sample of interes...
48 rxns $275.00
C09010010 MethylTaq DNA polymerase
MethylTaq DNA polymerase is a high-performance Hot Start thermostable recombinant DNA polymerase. MethylTaq is an extremely robust modified Taq DNA polymeras...
250 units $260.00
C09010012 MethylTaq Plus 2X Master Mix
MethylTaq Plus 2X Master Mix is a ready-to-use cocktail containing all components required for the amplification of bisulfiteconvertedNGS libraries, except p...
135 μL $120.00
C02030042 Premium Methyl UDI-UMI Adapters Module - 96 rxns
Premium Methyl UDI-UMI Adapters Module - 96 rxns includes 96 methylated full-length adapters with unique dual indexes (UDI) and uniq...
96 rxns $950.00
C02030040 Premium Methyl UMI-UDI Adapters - Set A
(Coming soon)Premium Methyl UMI-UDI Adapters - Set A includes methylated full-length adapters with 24 unique dual indexes (UDI) and ...
24 rxns $250.00
C02030041 Premium Methyl UMI-UDI Adapters - Set B
(Coming soon)Premium Methyl UMI-UDI Adapters - Set A includes methylated full-length adapters with 24 unique dual indexes (UDI) and ...
24 rxns $250.00

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