Diagenode

Premium Bisulfite kit

Catalog Number
Format
Price
C02030030
50 rxns
$200.00

Make your Bisulfite conversion now in only 90 minutes !

Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.

  •  Documents
    Premium Bisulfite kit MANUAL
    Fastest method for complete bisulfite conversion of DNA for methylation analysis. ...
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  •  Publications

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    Developmental regulation of DNA cytosine methylation at the immunoglobulin heavy chain constant locus.
    Oudinet C, Braikia FZ, Dauba A, Santos JM, Khamlichi AA
    DNA cytosine methylation is involved in the regulation of gene expression during development and its deregulation is often associated with disease. Mammalian genomes are predominantly methylated at CpG dinucleotides. Unmethylated CpGs are often associated with active regulatory sequences while methylated CpGs are of...

    Reproducibility of methylated CpG typing with the Illumina MiSeq
    Kampmann M.L. et al.
    DNA methylation patterns may be used for identification of body fluids and for age estimation of human individuals. We evaluated some of the challenges and pitfalls of studying methylated CpG sites. We compared the methylated CpG analysis of two different methods 1) massively parallel sequencing (MPS) using the Il...

    Faithful SGCE imprinting in iPSC-derived cortical neurons: an endogenous cellular model of myoclonus-dystonia
    Grütz K. et al.
    In neuropathology research, induced pluripotent stem cell (iPSC)-derived neurons are considered a tool closely resembling the patient brain. Albeit in respect to epigenetics, this concept has been challenged. We generated iPSC-derived cortical neurons from myoclonus-dystonia patients with mutations (W100G and R102X)...

    Regulation of Hox orthologues in the oyster Crassostrea gigas evidences a functional role for promoter DNA methylation in an invertebrate.
    Saint-Carlier E, Riviere G
    DNA methylation within promoter regions (PRDM) controls vertebrate early gene transcription and thereby development, but is neglected outside this group. However, epigenetic features in the oyster Crassostrea gigas suggest functional significance of PDRM in invertebrates. To investigate this, reporter constructs con...

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