Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate steps, and results in high yields of DNA ready for downstream analysis methods including PCR and Next-Generation Sequencing.
バイサルファイト処理
Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylate... Read more
エピジェネティクス・DNAメチル化
DNAメチル化研究のための完全打開策
Diagenodeは、DNAメチル化の分野の経験者、未経験者どちらの方でも、実験の一貫性のあるデータを確保しながら、できるだけシンプルかつ便利な実験にするために必要なすべてをご提供いたします。Diagenodeは、超音波装置、試薬キット、高品質の抗体、ハイスループットの自動化機能を備えており、特定のDNAメチル化解析のすべての要件に対応します。
DNAメチル化は最初に発見されたエピジェネティクスマークであり、エピジェネティクスス... Read more
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Developmental regulation of DNA cytosine methylation at the immunoglobulin heavy chain constant locus. Oudinet C, Braikia FZ, Dauba A, Santos JM, Khamlichi AA DNA cytosine methylation is involved in the regulation of gene expression during development and its deregulation is often associated with disease. Mammalian genomes are predominantly methylated at CpG dinucleotides. Unmethylated CpGs are often associated with active regulatory sequences while methylated CpGs are of...
Reproducibility of methylated CpG typing with the Illumina MiSeq Kampmann M.L. et al.
DNA methylation patterns may be used for identification of body fluids and for age estimation of human individuals. We evaluated some of the challenges and pitfalls of studying methylated CpG sites. We compared the methylated CpG analysis of two different methods 1) massively parallel sequencing (MPS) using the Il...
Faithful SGCE imprinting in iPSC-derived cortical neurons: an endogenous cellular model of myoclonus-dystonia Grütz K. et al. In neuropathology research, induced pluripotent stem cell (iPSC)-derived neurons are considered a tool closely resembling the patient brain. Albeit in respect to epigenetics, this concept has been challenged. We generated iPSC-derived cortical neurons from myoclonus-dystonia patients with mutations (W100G and R102X)...
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