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DNAメチル化

DNAメチル化についての理解は、転写の制御、ゲノムインプリンティング、胚発生、および発癌の研究に不可欠です。 Diagenodeは、シーケンシングまたはqPCRによるバイサルファイト変換と免疫沈降(IP)を提案しており、これらはDNAメチル化研究の2つの主要なアプローチです。

Diagenode製品ソリューション

Diagenodeテクノロジー

  • バイサルファイト変換


    Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level. Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.

    How it works

    Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figures).

    ADVANTAGES

    • Single nucleotide resolution
    • Gene-specific and genome-wide analyses
    • NGS compatible

    Downstream analysis techniques

  • メチル結合ドメインタンパク質

    The MBD technology used in our MethylCap Kit is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. The H6-GST-MBD fusion protein can be used to specifically isolate DNA containing methylated CpGs.

    Diagenode’s MethylCap Kit enables high enrichment of double-stranded DNA and a differential fractionation in function of the methylated CpG density. Fractionation reduces the complexity of samples and makes subsequent next generation sequencing easier. Prior to the MethylCap assay, DNA is first extracted and sheared using the Bioruptor® Sonicator.

    ADVANTAGES

    • Unaffected DNA
    • Robust & reproducible technique
    • NGS compatible

  • メチル化DNA免疫沈降

    The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.

    How it works

    In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.

    Overview


        QPCR

        NGS

    ADVANTAGES

    • Unaffected DNA
    • High enrichment yield
    • Robust & reproducible techniques
    • NGS compatible

    Find the solution you need

    Whole Genome Bisulfite-Seq
    Reduce Representation Bisulfite-Seq
    Bisulfite Conversion with qPCR, Sanger or Pyros
    Immunoprecipitation with Antibodies
    Immunoprecipitation with methyl-binding domain

バイサルファイト変換

カタログ番号製品フォーマット価格
C02030030 Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
50 rxns $214.00
C02030032 Premium Reduced Representation Bisulfite Sequencing (RRBS) kit
Reduced Representation Bisulfite Sequencing (RRBS)は単一ヌクレオチドレベルで、全ゲノムバイサルファイトシーケンシングに関連するDNAメチル化を効率的また、低コストで分析する非常に有効的な方法です。 制限MspI酵素(CCGG標的部位)およびサイズ選択を用いてゲノム...
24 rxns $1,561.00
C02030033 Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit
Reduced Representation Bisulfite Sequencing (RRBS)は単一ヌクレオチドレベルで、全ゲノムバイサルファイトシーケンシングに関連するDNAメチル化を効率的また、低コストで分析する非常に有効的な方法です。 制限MspI酵素(CCGG標的部位)およびサイズ選択を用いてゲノム...
96 rxns $4,177.00
C02030034 Premium Whole Genome Bisulfite Sequencing (WGBS) kit
Premium Whole Genome Bisulfite Sequencing(WGBS)キットは、イルミナ®プラットフォームを使用したシーケンシングのシングルおよびペアエンドのバイサルファイト変換DNAライブラリー調製専用に設計されています。 ChIP-Bis-Sequencingを実施するために、...
8 rxns $796.00
C02030031 Auto Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
40 rxns $214.00
C02030035 Bisulfite conversion reagent for RRBS
This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S...
8 rxns $107.00
C02040019 DNA methylation control package V2
The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls that can be added directly to the DNA sample of interes...
48 rxns $270.00
View more products from the バイサルファイト変換 product category...

メチル化DNA免疫沈降

カタログ番号製品フォーマット価格
C02010021 MagMeDIP qPCR kit
Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo...
48 rxns $658.00
C02010040 MagMeDIP-seq Package
MeDIP(メチル化DNA免疫沈降)の後にシーケンスを実行し、5-メチルシトシン抗体と磁気ビーズを使用してサンプルのDNAメチル化状態を検出します。 当社のキットには、最適化されたユーザーフレンドリーなプロトコールでメチル化DNAの最高濃縮を実現する、高品質の試薬が含まれています。 このパッケージには、cDNA...
10 rxns $1,392.00
C02010041 MagMeDIP-seq Pacakge V2
(Coming soon)Perform MeDIP-seq experiments, i.e. methylated DNA immunoprecipitation followed by next generation sequencing, to obtain reg...
10 rxns
C02010014 Auto MagMeDIP kit
Perform Automated MeDIP (seq) (Methylated DNA Immunoprecipitation sequencing) with Diagenode´s Auto MagMeDIP kit to estimate DNA methylation status of ...
48 rxns $658.00
C02040012 DNA Methylation control package
The DNA Methylation control package includes unmethylated and in vitro methylated DNA together with specific primer sets for assessing the efficiency of your...
40 rxns $270.00
C02040019 DNA methylation control package V2
The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls that can be added directly to the DNA sample of interes...
48 rxns $270.00

Methylbinding domain proteinアッセイ

カタログ番号製品フォーマット価格
C02020010 MethylCap kit
MethylCapキットを用いたアッセイは、ヒトMeCP2タンパク質のメチルCpG結合ドメイン(MBD)を使用したメチル化DNAのアフィニティー精製に基づき、メチル化されたCpGを含むDNAフラグメントを特異的にキャプチャーします。手順は手動プロセスまたはIP-Star®コンパクト自動システムの両方で行え...
48 rxns $612.00
C02020011 Auto MethylCap kit
The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methyl binding domain) protein . The Diagenode’s Auto MethylCa...
48 rxns $612.00
C02020012 MethylCap protein
メチル化CpGを含むDNAを特異的に単離するには、H6-GST-MBD融合タンパク質を用います。このたんぱく質はN末端His6タグを含むグルタチオン-S-トランスフェラーゼ(GST)とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン(MBD)から構成されています。*このキットで溶解およびせん断することがで...
100 µg $500.00

DNA methylation standard

カタログ番号製品フォーマット価格
C02040018 DNA hydroxymethylation control package
The DNA hydroxymethylation control package includes one hydroxymethylated spike-in control and its corresponding qPCR primer pair. The control can be ad...
48 rxns $203.00
C02040012 DNA Methylation control package
The DNA Methylation control package includes unmethylated and in vitro methylated DNA together with specific primer sets for assessing the efficiency of your...
40 rxns $270.00
C02040019 DNA methylation control package V2
The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls that can be added directly to the DNA sample of interes...
48 rxns $270.00

PCR enzymes and nucleotides

カタログ番号製品フォーマット価格
C11000002 Hydroxymethyl dCTP
Hydroxymethyl dCTP can be used as a substrate for MethylTaq DNA polymerase (Cat. No. AF-103-0250) permitting the amplification of DNA containi...
250 µl
C09010010 MethylTaq DNA polymerase
MethylTaq DNAポリメラーゼは、高性能ホットスタート熱安定性組換え型DNAポリメラーゼです。 MethylTaqは、74℃以下のすべての活性を完全に欠如しているため、低温での非特異的プライミングを回避する、非常に頑強に修飾されたTaq DNAポリメラーゼです。 この強力な酵素は、要求の厳しい用途でも優れ...
250 units $235.00
C09010012 MethylTaq Plus 2X Master Mix
MethylTaq Plus 2X Master Mix is a ready-to-use cocktail containing all components required for the amplification of bisulfiteconvertedNGS libraries, except p...
135 μL $105.00

Hydroxymethylated DNA Immunoprecipitation

カタログ番号製品フォーマット価格
C02010031 hMeDIP kit x16 (monoclonal mouse antibody)
このキットは、5-ヒドロキシメチルシトシン(5-hmC)(20µl, 50µl, 100µl)に対する最初で唯一のモノクローナル抗体を用いたDNA免疫沈降実験専用に設計されています。キットには、免疫沈降の効率を評価するコントロール配列(5-hmC、5-mCお...
16 rxns $546.00
C02010034 Auto hMeDIP kit x16 (monoclonal mouse antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy...
16 rxns $546.00
C02040018 DNA hydroxymethylation control package
The DNA hydroxymethylation control package includes one hydroxymethylated spike-in control and its corresponding qPCR primer pair. The control can be ad...
48 rxns $203.00

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