Diagenode

キット

DNAメチル化

DNAメチル化研究のための完全打開策

Diagenodeは、DNAメチル化の分野の経験者、未経験者どちらの方でも、実験の一貫性のあるデータを確保しながら、できるだけシンプルかつ便利な実験にするために必要なすべてをご提供いたします。Diagenodeは、超音波装置、試薬キット、高品質の抗体、ハイスループットの自動化機能を備えており、特定のDNAメチル化解析のすべての要件に対応します。

  • バイサルファイト処理: 亜硫酸水素塩による化学修飾
  • MeDIPおよびMBD: メチル化DNAの収穫
  • メチル化感受性または依存性制限酵素処理

  • さらに詳しく
    Description Features
    Bisulfite conversion Chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at single nucleotide resolution.
    • Single nucleotide resolution
    • Quantitative analysis - methylation rate (%)
    • Gold standard and well studied
    • Compatible with automation
    Methylated DNA enrichment (Hydroxy-)Methylated DNA is enriched by using specific antibodies (hMeDIP or MeDIP) or proteins (MBD) that specifically bind methylated CpG sites in fragmented genomic DNA.
    • Resolution depends on the fragment size of the enriched methylated DNA (300 bp)
    • Qualitative analysis
    • Compatible with automation
    Restriction enzyme-based digestion Use of (hydroxy)methylation-sensitive or (hydroxy)methylation-dependent restriction enzymes for DNA methylation analysis at specific sites.
    • Determination of methylation status is limited by the enzyme recognition site
    • Easy to use

    5-methylcytosine (5-mC) has been known for a long time as the only modification of DNA for epigenetic regulation. In 2009, however, Kriaucionis discovered a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC). The so-called 6th base, is generated by enzymatic conversion of 5-methylcytosine (5-mC) into 5-hydroxymethylcytosine by the TET family of oxygenases. Early reports suggested that 5-hmC may represent an intermediate of active demethylation in a new pathway which demethylates DNA, converting 5-mC to cytosine. Recent evidence fuel this hypothesis suggesting that further oxidation of the hydroxymethyl group leads to a formyl or carboxyl group followed by either deformylation or decarboxylation. The formyl and carboxyl groups of 5-formylcytosine (5-fC) and 5-carboxylcytosine (5-caC) could be enzymatically removed without excision of the base.

関連商品

Bisulfite conversion

Cat. No.ProductFormatPrice
C02030030 Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
50 rxns $195.00
C02030032 Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit
Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level withou...
24 rxns $1,400.00
C05010033 Premium WGBS Indexes - 12
Theses 12 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
96 rxns $1,080.00
C05010034 Premium WGBS Indexes - 24
Theses 24 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
192 rxns $1,940.00
C05010032 Premium WGBS Indexes - 6
Theses 6 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite ...
48 rxns $630.00
C02030034 Premium Whole Genome Bisulfite Sequencing (WGBS) kit
The Premium Whole Genome Bisulfite Sequencing (WGBS) Kit is designed to prepare single and paired-end bisulfite converted DNA libraries for sequencing using ...
8 rxns $720.00
C02030031 Auto Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
40 rxns $195.00
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Methylated DNA Immunoprecipitation

Cat. No.ProductFormatPrice
C02010011 Auto MeDIP kit
Perform Automated MeDIP (Methylated DNA Immunoprecipitation[application MeDIP] to estimate DNA methylation status of your sample using the 5-methylcytidine a...
16 rxns $450.00
C02010021 MagMeDIP kit
Perform MeDIP (Methylated DNA Immunoprecipitation) to estimate DNA methylation status of your sample using the 5-methylcytidine antibody. The development of ...
48 rxns $595.00

Hydroxymethylated DNA Immunoprecipitation

Cat. No.ProductFormatPrice
C02010031 hMeDIP kit x16 (monoclonal mouse antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto...
16 rxns $495.00
C02010034 Auto hMeDIP kit x16 (monoclonal mouse antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy...
16 rxns $495.00
C02010033 Auto hMeDIP kit x16 (monoclonal rat antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy...
16 rxns $495.00
C02010035 Auto hMeDIP kit x16 (polyclonal rabbit antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using an antibody against 5-hydroxymethylcytosine (5-hmC) on our Automated Sy...
16 rxns $495.00
C02010030 hMeDIP kit x16 (monoclonal rat antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto...
16 rxns $495.00
C02010032 hMeDIP kit x16 (polyclonal rabbit antibody)
This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcyto...
16 rxns $495.00

Methylbinding domain protein assay

Cat. No.ProductFormatPrice
C02020010 MethylCap kit
The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methylbinding domain) protein . This procedure has first been optimi...
48 rxns $550.00
C02020011 Auto MethylCap kit
The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methyl binding domain) protein . The Diagenode’s Auto MethylCa...
48 rxns $495.00
C02020012 MethylCap protein
The H6-GST-MBD fusion protein can be used to specifically isolate DNA containing methylated CpGs. It consists of the methyl binding domain (MBD) of human MeC...
50 rxns $450.00

DNA methylation standard

PCR enzymes and nucleotides

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