This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcytosine (5-hmC )(20µl, 50µl, 100µl) available in the market. It includes control sequences (5-hmC, 5-mC and unmodified cytosine DNA standards) and control primer pairs (internal and external controls), to assess the effiency of your immunoprecipitation.
Methylated DNA immunoprecipitation
The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.
How it works
In brief, Methyl DNA IP is ... Read more
Diagenode strongly recommends using this: hMeDIP kit x16 (monoclonal mouse antibody) (Diagenode Cat# C02010031). Click here to copy to clipboard.
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Transcription of synaptic plasticity-related genes in patients with somnipathy combined with type 2 diabetes Yi Zhang, Rui Ma, Shaohong Zou, Gaiyu Tong, Gulibakeranmu Abula, Manna Hu, Qing Dai Objective: To investigate DNA methylation and hydroxymethylation in patients with somnipathy combined with type 2 diabetes, and to explore the effects of DNA methylation and hydroxymethylation on gene expression.
Methods: Thirty patients with somnipathy combined with type 2 diabetes and 20 patients with type-2 diab...
Coordinate Regulation of TET2 and EBNA2 Control DNA Methylation State of Latent Epstein-Barr Virus Lu F. et al. Epstein-Barr Virus (EBV) latency and its associated carcinogenesis are regulated by dynamic changes in DNA methylation of both virus and host genomes. We show here that the Ten-Eleven Translocation 2 (TET2) gene, implicated in hydroxymethylation and active DNA demethylation, is a key regulator of EBV latency type DN...
Decoupling of DNA methylation and activity of intergenic LINE-1 promoters in colorectal cancer Vafadar-Isfahani N. et al. Hypomethylation of LINE-1 repeats in cancer has been proposed as the main mechanism behind their activation; this assumption, however, was based on findings from early studies that were biased toward young and transpositionally active elements. Here, we investigate the relationship between methylation of 2 intergeni...
Maternal immune activation induces GAD1 and GAD2 promoter remodeling in the offspring prefrontal cortex Labouesse MA et al. Maternal infection during pregnancy increases the risk of neurodevelopmental disorders in the offspring. In addition to its influence on other neuronal systems, this early-life environmental adversity has been shown to negatively affect cortical γ-aminobutyric acid (GABA) functions in adult life, including imp...
Hepatic DNA hydroxymethylation is site-specifically altered by chronic alcohol consumption and aging Tammen SA et al.
PURPOSE:
Global DNA hydroxymethylation is markedly decreased in human cancers, including hepatocellular carcinoma, which is associated with chronic alcohol consumption and aging. Because gene-specific changes in hydroxymethylcytosine may affect gene transcription, giving rise to a carcinogenic environment, we det...
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