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hMeDIP kit x16 (monoclonal mouse antibody)

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Corticosteroid receptors adopt distinct cyclical transcri...

ENDOCRINOLOGY
Catalog Number
Format
Price
C02010031
(AF-110-0016)
16 rxns
$510.00

This kit has been designed to perform Hydroxymethylated DNA Immunoprecipitation using the first and only monoclonal antibody against 5-hydroxymethylcytosine (5-hmC )(20µl, 50µl, 100µl) available in the market. It includes control sequences (5-hmC, 5-mC and unmodified cytosine DNA standards) and control primer pairs (internal and external controls), to assess the effiency of your immunoprecipitation.

  • Characteristics
    • Immunoprecipiation performed with monoclonal antibody against 5-hmC
    • Including internal & external controls for QC of each reaction
      • Mouse primers against Sf1 (hydroxymethylated gene in mouse)
      • 5-hmC, 5-mC and unmethylated DNA
    • Complete magnetic bead based kit
    • All in one tube
  •  Applications
    Methylated DNA immunoprecipitation
    The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP. How it works In brief, Methyl DNA IP is ... Read more
    DNA Methylation
    Complete solutions for DNA methylation studies Whether you are experienced or new to the field of DNA methylation, Diagenode has everything you need to make your assay as easy and convenient as possible while ensuring consistent data betwee... Read more
  •  Documents
    hMeDIP kit MANUAL
    The Diagenode hMeDIP kit is designed to immunoprecipitate hydroxymethylated DNA (hMethyl ...    		 Download
    Exclusive Highly Specific Kits Antibodies for DNA HydroxyMethylation Studies POSTER
    5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA by two US groups (K...    		 Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: hMeDIP kit x16 (monoclonal mouse antibody) (Diagenode Cat# C02010031). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Transcription of synaptic plasticity-related genes in patients with somnipathy combined with type 2 diabetes
    Yi Zhang, Rui Ma, Shaohong Zou, Gaiyu Tong, Gulibakeranmu Abula, Manna Hu, Qing Dai
    Objective: To investigate DNA methylation and hydroxymethylation in patients with somnipathy combined with type 2 diabetes, and to explore the effects of DNA methylation and hydroxymethylation on gene expression. Methods: Thirty patients with somnipathy combined with type 2 diabetes and 20 patients with type-2 diab...

    Emerging Role of One-Carbon Metabolism and DNA Methylation Enrichment on δ-Containing GABAA Receptor Expression in the Cerebellum of Subjects with Alcohol Use Disorders (AUD
    Gatta E. et al.
    Background Cerebellum is an area of the brain particularly sensitive to the effects of acute and chronic alcohol consumption. Alcohol exposure decreases cerebellar Purkinje cell output by increasing GABA release from Golgi cells onto extrasynaptic α6/δ-containing GABAA receptors located on glutamate...

    Coordinate Regulation of TET2 and EBNA2 Control DNA Methylation State of Latent Epstein-Barr Virus
    Lu F. et al.
    Epstein-Barr Virus (EBV) latency and its associated carcinogenesis are regulated by dynamic changes in DNA methylation of both virus and host genomes. We show here that the Ten-Eleven Translocation 2 (TET2) gene, implicated in hydroxymethylation and active DNA demethylation, is a key regulator of EBV latency type DN...

    Decoupling of DNA methylation and activity of intergenic LINE-1 promoters in colorectal cancer
    Vafadar-Isfahani N. et al.
    Hypomethylation of LINE-1 repeats in cancer has been proposed as the main mechanism behind their activation; this assumption, however, was based on findings from early studies that were biased toward young and transpositionally active elements. Here, we investigate the relationship between methylation of 2 intergeni...

    Maternal immune activation induces GAD1 and GAD2 promoter remodeling in the offspring prefrontal cortex
    Labouesse MA et al.
    Maternal infection during pregnancy increases the risk of neurodevelopmental disorders in the offspring. In addition to its influence on other neuronal systems, this early-life environmental adversity has been shown to negatively affect cortical γ-aminobutyric acid (GABA) functions in adult life, including imp...

    Hepatic DNA hydroxymethylation is site-specifically altered by chronic alcohol consumption and aging
    Tammen SA et al.
    PURPOSE: Global DNA hydroxymethylation is markedly decreased in human cancers, including hepatocellular carcinoma, which is associated with chronic alcohol consumption and aging. Because gene-specific changes in hydroxymethylcytosine may affect gene transcription, giving rise to a carcinogenic environment, we det...

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