DNA methylation

Methylated DNA immunoprecipitation

The Methylated DNA Immunoprecipitation is based on the affinity purification of methylated and hydroxymethylated DNA using, respectively, an antibody directed against 5-methylcytosine (5-mC) in the case of MeDIP or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.

How it works

In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.

Applications

qPCR analysis

NGS analysis

Advantages

Unaffected DNA
High enrichment yield
Robust & reproducible techniques
NGS compatible

Products

Cat. No.	Product	Format	Price
C02010021	MagMeDIP qPCR Kit Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo...	48 rxns (IP)	$670.00
C02010041	MagMeDIP-seq Package V2 MeDIP-seq library prep. kit for low DNA amount Perform MeDIP-seq experiments, i.e. methylated DNA immunoprecipitation followed by next generation sequencing, to obtain region-resolutio...	10 rxns	$1,850.00
C02010014	Auto MagMeDIP qPCR Kit Perform MeDIP (Methylated DNA Immunoprecipitation) on the SX-8G IP-Star® Automated System followed by qPCR to estimate DNA methy...	48 rxns	$670.00
C02040012	DNA methylation control package The DNA methylation control package includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer se...	40 rxns	$275.00
C02040019	DNA methylation control package V2 The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer sets that...	48 rxns	$250.00
C02010040	MagMeDIP-seq Package Discover our NEW MagMeDIP-seq Package V2. An updated version of this package is available for superior performance with lower DNA amount. Perform...	10 rxns		Get a quote You are about to request a quote for MagMeDIP-seq Package. Fill out the form below and we will be in touch with you very soon. All * fields are mandatory Contact Information First name ^* Last name ^* Company ^* Phone number City Country ^* State Email ^* Email verification^* Comment ×

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