Diagenode

DNA methylation

Methylated DNA Immunoprecipitation

The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.

How it works

In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.

Overview


    qPCR

    NGS

ADVANTAGES

  • Unaffected DNA
  • High enrichment yield
  • Robust & reproducible techniques
  • NGS compatible

Find the solution you need

Whole Genome Bisulfite-Seq
Reduce Representation Bisulfite-Seq
Bisulfite Conversion with qPCR, Sanger or Pyros
Immunoprecipitation with Antibodies
Immunoprecipitation with methyl-binding domain

Products

Cat. No.ProductFormatPrice
C02010021 MagMeDIP qPCR Kit
Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo...
48 rxns $615.00
C02010040 MagMeDIP-seq Package
*Use the MAGPACK02 promo code in your online order or by clicking on the banner. Sensitive tumour detection and classification using plasma cel...
10 rxns $1,299.00
C02010014 Auto MagMeDIP qPCR Kit
Perform MeDIP (Methylated DNA Immunoprecipitation) on the SX-8G IP-Star® Automated System followed by qPCR to estimate DNA methy...
48 rxns $615.00
C02010011 Auto MeDIP kit - New reference C02010013
Please refer to Auto MagMeDIP kit Perform Automated MeDIP (seq) (Methylated DNA Immunoprecipitation sequencing) to estimate DNA methylation status of your s...
16 rxns

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