The Methylated DNA Immunoprecipitation is based on the affinity purification of methylated and hydroxymethylated DNA using, respectively, an antibody directed against 5-methylcytosine (5-mC) in the case of MeDIP or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.
In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.
| Cat. No. | Product | Format | Price | |
|---|---|---|---|---|
| C02010021 |
MagMeDIP qPCR Kit Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methylo... |
48 rxns | $658.00 | |
| C02010040 |
MagMeDIP-seq Package Sensitive tumour detection and classification using plasma cell-free DNA methylomesRead the publication Preparation of cfMeDIP-seq libraries for methy... |
10 rxns | $1,392.00 | |
| C02010014 |
Auto MagMeDIP qPCR Kit Perform MeDIP (Methylated DNA Immunoprecipitation) on the SX-8G IP-Star® Automated System followed by qPCR to estimate DNA methy... |
48 rxns | $658.00 | |
| C02040012 |
DNA methylation control package The DNA methylation control package includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer se... |
40 rxns | $270.00 | |
| C02040019 |
DNA methylation control package V2 The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer sets that... |
48 rxns | $270.00 |
| Cat. No. | Product | Format | Price | |
|---|---|---|---|---|
| C02010031 |
hMeDIP kit x16 (monoclonal mouse antibody) The hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation analysis.... |
16 rxns | $546.00 | |
| C02010034 |
Auto hMeDIP kit x16 (monoclonal mouse antibody) The Auro hMeDIP kit is designed for enrichment of hydroxymethylated DNA from fragmented genomic DNA samples for use in genome-wide methylation anal... |
16 rxns | $546.00 | |
| C02040018 |
DNA hydroxymethylation control package The DNA hydroxymethylation control package includes one hydroxymethylated spike-in control and its corresponding qPCR primer set that can be added to the DNA... |
48 rxns | $203.00 |
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