The Methylated DNA IP (MeDIP) is based on the affinity purification of methylated DNA using an antibody directed against 5-methylcytosine (5-mC) or 5-hydroxymethylcytosine (5-hmC) in the case of hMeDIP.
In brief, Methyl DNA IP is performed as follows: Genomic DNA from cultured cells or tissues is prepared, sheared, and then denatured. Then, immunoselection and immunoprecipitation can take place using the antibody directed against 5 methylcytosine and antibody binding beads. After isolation and purification is performed, the IP’d methylated DNA is ready for any subsequent analysis as qPCR, amplification, hybridization on microarrays or next generation sequencing.
| Cat. No. | Product | Format | Price | |
|---|---|---|---|---|
| C02010021 |
MagMeDIP qPCR Kit Perform MeDIP (Methylated DNA Immunoprecipitation) followed by qPCR to estimate DNA methylation status of your sample using 5-methylcytosine antibo... |
48 rxns | $615.00 | |
| C02010014 |
Auto MagMeDIP qPCR Kit Perform MeDIP (Methylated DNA Immunoprecipitation) on the SX-8G IP-Star® Automated System followed by qPCR to estimate DNA methy... |
48 rxns | $615.00 | |
| C02010011 |
Auto MeDIP kit - New reference C02010013 Please refer to Auto MagMeDIP kit Perform Automated MeDIP (seq) (Methylated DNA Immunoprecipitation sequencing) to estimate DNA methylation status of your s... |
16 rxns | $0.00 |
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