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Antibodies

ChIP-seq grade antibodies

Unparalleled ChIP-Seq results with the most rigorously validated antibodies

Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.

ChIP-seq profile of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.

ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region.

Diagenode’s highly validated antibodies:

  • Highly sensitive and specific
  • Cost-effective (requires less antibody per reaction)
  • Batch-specific data is available on the website
  • Expert technical support
  • Sample sizes available
  • 100% satisfaction guarantee

Products

Cat. No.ProductApplicationPurity
C15310266 AF9 polyclonal antibody
(100 μl) 		ChIP-qPCR ChIP-seq Whole antiserum from rabbit containing 0.05% azide.
C15310197 AML1-ETO polyclonal antibody - Classic
(100 µl) 		ChIP-qPCR ChIP-seq ELISA WB Whole antiserum
C15310002 CBFb polyclonal antibody - Classic
(100 µl) 		ChIP-qPCR ChIP-seq ELISA Whole antiserum
C15410224-100 CBP polyclonal antibody - Classic
(100 μl) 		ChIP-qPCR ChIP-seq IF IHC IP WB Affinity purified
C15410333 CBX8 polyclonal antibody
(100 μl) 		ChIP-qPCR ChIP-seq IP WB Affinity purified
C15410334 CHD1 polyclonal antibody
(100 μl) 		ChIP-qPCR ChIP-seq IP WB Affinity purified
C15410210-50 CTCF polyclonal antibody - Classic
(50 μg) 		ChIP-qPCR ChIP-seq ELISA IF siRNA Knockdown WB Affinity purified
C15410315 CXXC1 polyclonal antibody - Classic
(50 μg /50 μl) 		ChIP-qPCR ChIP-seq IF WB Affinity purified
C15410314 E2F6 polyclonal antibody - Classic
(50 μg /50 μl) 		ChIP-qPCR ChIP-seq IF WB Affinity purified
C15100066 ER alpha monoclonal antibody - Classic
(100 µl) 		ChIP-qPCR ChIP-seq ELISA GSA IC IP WB Ascites fluid
C15310001 ETO polyclonal antibody - Classic
(100 µl) 		ChIP-qPCR ChIP-seq ELISA Whole antiserum
C15410039-50 EZH2 polyclonal antibody - Classic
(50 µg/50 µl) 		ChIP-qPCR ChIP-seq IC siRNA Knockdown WB Protein G purified
C15410231-100 FOXA1 polyclonal antibody - Classic
(100 μl) 		ChIP-qPCR ChIP-seq IF IHC IP WB Affinity purified
C15410232-100 FOXM1 polyclonal antibody - Classic
(100 μl) 		ChIP-qPCR ChIP-seq IHC WB Affinity purified
C15200010-50 GR monoclonal antibody - Classic
(50 µg/50 µl) 		ChIP-qPCR ChIP-seq ELISA Flow Cyt GSA IP WB Protein A/G purified
C15410201 H2A.Z polyclonal antibody - Premium
(50 μg/33 μl
(50 ChIP reactions)) 		ChIP-qPCR ChIP-seq ELISA IF WB Affinity purified
C15410173 H2A.Zac polyclonal antibody - Classic
(50 μg/72 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF Affinity purified
C15410202-050 H2A.Zac polyclonal antibody - Premium
(50 μg/46 μl
(50 ChIP reactions)) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15210009 H2A.ZK4ac monoclonal antibody
(100 µg/ 100 µl) 		ChIP-qPCR ChIP-seq IF WB Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
C15410215 H2AK5ac polyclonal antibody - Classic
(50 μg/ 54 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410212 H2BK12ac polyclonal antibody - Classic
(50 μg/ 61 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410220 H2BK15ac polyclonal antibody - Classic
(50 μg/ 24 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15210010 H2BK20ac monoclonal antibody
(100 µg/ 100 µl) 		ChIP-qPCR ChIP-seq IF WB Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
C15210011 H3.3 monoclonal antibody
(100 µg/ 100 µl) 		ChIP-qPCR ChIP-seq WB Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
C15210005 H3K14ac monoclonal antibody - Classic
(100 µg /100 µl) 		ChIP-qPCR ChIP-seq DB IF WB Protein A purified monoclonal antibody in PBS containing 1% BSA and 0.09% azide.
C15410139 H3K18ac polyclonal antibody - Classic
(50 μg/62 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15210007 H3K23me2 monoclonal antibody
(100 µg/ 100 µl) 		ChIP-qPCR ChIP-seq WB Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
C15410174 H3K27ac polyclonal antibody - Classic
(50 μg/42 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410196 H3K27ac polyclonal antibody - Premium
(50 μg/18 μl
(50 ChIP reactions)) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410069 H3K27me3 polyclonal antibody - Classic
(50 μg/34 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410195 H3K27me3 polyclonal antibody - Premium
(50 μg/27 μl
(50 ChIP reactions)) 		ChIP-qPCR ChIP-seq DB ELISA IF Peptide array WB Affinity purified
C15410091 H3K27me3S28p polyclonal antibody - Classic
(50 μg/ 295 μl) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
C15410307 H3K36ac polyclonal antibody - Classic
(50 μg) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
C15310127 H3K36me2 polyclonal antibody - Classic
(100 µl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Whole antiserum
C15310058 H3K36me3 polyclonal antibody - Classic
(100 µl) 		ChIP-qPCR ChIP-seq DB ELISA WB Whole antiserum
C15410058 H3K36me3 polyclonal antibody - Classic
(50 µg/56 µl) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
C15410192 H3K36me3 polyclonal antibody - Premium
(50 μg/42 μl
(50 ChIP reactions)) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410322 H3K4ac polyclonal antibody - Classic
(50 μg/46 μl) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
C15410037-50 H3K4me1 polyclonal antibody - Classic
(50 µg/18 µl) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
C15410194 H3K4me1 polyclonal antibody - Premium
(50 μg/54 μl
(50 ChIP reactions)) 		ChIP-qPCR ChIP-seq DB ELISA IF Peptide array WB Affinity purified
C15410035 H3K4me2 polyclonal antibody - Classic
(50 μg/42 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15200152 H3K4me3 monoclonal antibody - Classic
(50 µg/50 µl) 		ChIP-qPCR ChIP-seq ELISA IF WB Protein A/G purified
C15410003-50 H3K4me3 polyclonal antibody - Premium
(50 µg/36 µl
(50 ChIP reactions)) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410213 H3K56ac polyclonal antibody - Classic
(50 μg/ 84 μl) 		ChIP-qPCR ChIP-seq ELISA WB Affinity purified
C15210006 H3K79ac monoclonal antibody - Classic
(100 µg /100 µl) 		ChIP-qPCR ChIP-seq IF WB Protein A purified
C15410082 H3K79me1 polyclonal antibody - Classic
(50 μg/31 μl) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
C15410051 H3K79me2 polyclonal antibody - Classic
(50 μg/46 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410068 H3K79me3 polyclonal antibody - Classic
(50 µg/42 µl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410005 H3K9/14ac polyclonal antibody - Classic
(50 μg/36 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410200 H3K9/14ac polyclonal antibody - Premium
(50 μg/62 μl
(50 ChIP reactions)) 		ChIP-qPCR ChIP-seq DB ELISA IF Peptide array WB Affinity purified
C15410004 H3K9ac polyclonal antibody - Classic
(50 µg/37 µl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410177 H3K9ac polyclonal antibody - Classic
(50 μg/47 μl) 		ChIP-qPCR ChIP-seq DB ELISA WB Affinity purified
C15410056 H3K9me3 polyclonal antibody
(50 µg/56 µl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
C15410193 H3K9me3 polyclonal antibody - Premium
(50 μg/19 μl
(50 ChIP reactions)) 		ChIP-qPCR ChIP-seq DB ELISA IF Peptide array WB Affinity purified
C15500003-50 H3K9me3 recombinant antibody and negative control - Premium
(50 µg) 		ChIP-qPCR ChIP-seq IF affinity purified
C15410171 H3R17me2(asym)K18ac polyclonal antibody - Classic
(50 μg/53 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410331-50 H4K12ac polyclonal antibody
(50 μg/ 84 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15210008 H4K20ac monoclonal antibody
(100 µg/100 µl) 		ChIP-qPCR ChIP-seq IF WB Protein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
C15200147 H4K20me1 monoclonal antibody - Classic
(50 μg/23 μl) 		ChIP-qPCR ChIP-seq DB IF WB Protein A purified
C15410057 H4K20me3 polyclonal antibody - Classic
(50 μg/47 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410207 H4K20me3 polyclonal antibody - Premium
(50 μg/53 μl
(50 ChIP reactions)) 		ChIP-qPCR ChIP-seq DB ELISA IF Peptide array WB Affinity purified
C15410024 H4K5,8,12,16ac polyclonal antibody - Classic
(50 μg/66 μl) 		ChIP-qPCR ChIP-seq DB IF Affinity purified
C15410021 H4K5,8,12ac polyclonal antibody - Classic
(50 μg/66 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410025 H4K5ac polyclonal antibody - Classic
(50 μg/28 μl) 		ChIP-qPCR ChIP-seq DB ELISA IF WB Affinity purified
C15410053 HDAC1 polyclonal antibody - Classic
(50 µg/79 µl) 		ChIP-qPCR ChIP-seq ELISA IF WB Affinity purified
C15410325-50 HDAC1 polyclonal antibody - Premium
(50 μg/ 29 μl
(25 ChIP reactions)) 		ChIP-qPCR ChIP-seq ELISA IF Protein Analysis siRNA Knockdown WB Affinity purified
C15200201 HDAC2 monoclonal antibody - Classic
(50 μg/ 25 μl) 		ChIP-qPCR ChIP-seq IF siRNA Knockdown WB Protein A purified
C15410332 JMJD2a polyclonal antibody
(100 μl) 		ChIP-qPCR ChIP-seq IP WB Affinity purified
C15410236-100 KAP1 polyclonal antibody - Classic
(100 μl) 		ChIP-qPCR ChIP-seq IF IHC IP WB Affinity purified
C15410335 KAT2B polyclonal antibody
(100 μl) 		ChIP-qPCR ChIP-seq WB Affinity purified
C15410067 LSD1 polyclonal antibody - Classic
(50 µg/27 µl) 		ChIP-qPCR ChIP-seq ELISA IF siRNA Knockdown WB Affinity purified
C15210003 macroH2A.1/H2A.2 monoclonal antibody - Classic
(100 µg /100 µl) 		ChIP-qPCR ChIP-seq IF WB Protein A purified
C15310264 MLL1 polyclonal antibody
(100 µl) 		ChIP-qPCR ChIP-seq ELISA Whole antiserum from rabbit containing 0.05% azide.
C15310254 MYH11 polyclonal antibody - Classic
(100 μl) 		ChIP-qPCR ChIP-seq ELISA WB Whole antiserum
C15410240-100 NF-E2 polyclonal antibody - Classic
(100 μl) 		ChIP-qPCR ChIP-seq IF WB Affinity purified
C15310256 NFKB p65 polyclonal antibody - Classic
(100 μl) 		ChIP-qPCR ChIP-seq ELISA IHC WB Whole antiserum
C15310261 NFYA polyclonal antibody - Classic
(100 μl) 		ChIP-qPCR ChIP-seq IHC WB Whole antiserum
C15410241-100 NFYB polyclonal antibody - Classic
(100 μl) 		ChIP-qPCR ChIP-seq IF IP WB Affinity purified
C15200013 NRF1 monoclonal antibody - Classic
(50 μg/23 μl) 		ChIP-qPCR WB Protein A purified
C15410305 OCT4 polylconal antibody - Classic
(50 μg) 		ChIP-qPCR ChIP-seq ELISA WB Affinity purified
C15200211 p300 monoclonal antibody - Classic
(50 μg) 		ChIP-qPCR ChIP-seq Protein A purified
C15410083 p53 polyclonal antibody - Classic
(50 µg/50 µl) 		ChIP-qPCR ChIP-seq ELISA WB Affinity purified
C15100055-100 Pol II monoclonal antibody - Classic
(100 µl) 		ChIP-qPCR ChIP-seq siRNA Knockdown WB Ascites fluid
C15200004 Pol II monoclonal antibody - Classic
(50 μg/50 μl) 		ChIP-qPCR ChIP-seq ELISA IF siRNA Knockdown WB Protein A purified monoclonal antibody in PBS containing 0.05% azide.
C15200005-50 Pol II S2p monoclonal antibody - Classic
(50 μg/50 μl) 		ChIP-qPCR ChIP-seq ELISA IF siRNA Knockdown WB Affinity purified monoclonal antibody in PBS containing 0.05% azide.
C15200007-50 Pol II S5p monoclonal antibody - Classic
(50 μg/50 μl) 		ChIP-qPCR ChIP-seq ELISA IF siRNA Knockdown WB Affinity purified monoclonal antibody in PBS containing 0.05% azide.
C15310155 RARA polyclonal antibody - Classic
(100 µl) 		ChIP-qPCR ChIP-seq ELISA WB Whole antiserum
C15410313 RNF2 polyclonal antibody - Classic
(50 μg /50 μl) 		ChIP-qPCR ChIP-seq IF WB Affinity purified
C15410036 SAP30 polyclonal antibody - Classic
(50 µg/25 µl) 		ChIP-qPCR ChIP-seq WB Protein G purified
C15410250-100 SIN3A polyclonal antibody - Classic
(100 μl) 		ChIP-qPCR ChIP-seq IF IHC IP WB Affinity purified
C15410274 SMAD1 polyclonal antibody - Classic
(100 μg) 		ChIP-qPCR ChIP-seq WB Affinity purified
C15410317 SMARCB1 polyclonal antibody - Classic
(50 μg /50 μl) 		ChIP-qPCR ChIP-seq WB Affinity purified
C15200012 TAL1 monoclonal antibody - Classic
(50 μg/50 μl) 		ChIP-qPCR ChIP-seq IF Affinity purified
C15410266-100 TARDBP polyclonal antibody - Classic
(100 μl) 		ChIP-qPCR ChIP-seq IF IHC IP WB Affinity purified
C15200002 TBP monoclonal antibody - Classic
(100 µg) 		ChIP-qPCR ChIP-seq siRNA Knockdown WB Ammonium sulphate purified
C15410260-100 ZHX2 polyclonal antibody - Classic
(100 μl) 		ChIP-qPCR ChIP-seq IF IHC WB Affinity purified

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