NFYB Antibody

Catalog Number
100 μl
  Bulk order

Polyclonal antibody raised in rabbit against NFYB (nuclear transcription factor Y, beta), using a recombinant protein.

Concentration0.42 μg/μl
Species reactivityHuman, mouse
PurityAffinity purified
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 1% BSA, 20% glycerol and 0.025% proclin300.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 2 μg/IP Fig 1, 2
Western Blotting 1:500 - 1:3,000 Fig 3
Immunoprecipitation 2.5 μg/IP Fig 4
Immunofluorescence 1:100 - 1:1,000 Fig 5

* Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 μg per IP.

  • Validation Data

    NFYB Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against NFYB
    ChIP assays were performed using HeLa cells, the Diagenode antibody against NFYB (Cat. No. C15410241) and optimized primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2 and 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for the AURKA and GAPDH promoters, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    NFYB Antibody ChIP-seq Grade

    NFYB Antibody for ChIP-seq

    NFYB Antibody for ChIP-seq assay

    NFYB Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NFYB
    ChIP was performed on sheared chromatin from 4 million HeLa cells using 2 μg of the Diagenode antibody against NFYB (Cat. No. C15410241) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 2 Mb region of the human X chromosome (fig 2A and B), and in a two genomic regions surrounding the AURKA and GAPDH positive control genes.

    NFYB Antibody validated in Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against NFYB
    Nuclear extracts from Neuro2A (lane 1), C8D30 (lane 2), NIH3T3 (lane 3), Raw264.7 (lane 4) and C2C12 (lane 5) cells (30 µg) were analysed by Western blot using the Diagenode antibody against NFYB (cat. No. C15410241) diluted 1:1,000. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    NFYB Antibody validated in Immunoprecipitation

    Figure 4. Immunoprecipitation using the Diagenode antibody directed against NFYB
    Immunoprecipitation was performed on whole cell extracts from 293T cells using 2.5 μg of the Diagenode antibody against NFYB (Cat. No. C15410241). An equal amount of rabbit IgG was used as a negative control. The immunoprecipitated NFYB protein was detected by western blot with the NFYB antibody diluted 1:1,000. The IP with the NFYB antibody and with the IgG negative control are shown in lane 3 and lane 2, respectively. Lane 1 shows the input (40 μg of 293T whole cell extract).

    NFYB Antibody validated in Immunofluorescence

    Figure 5. Immunofluorescence with the Diagenode antibody directed against NFYB
    HeLa cells were fixed with formaldehyde and stained with the Diagenode antibody against NFYB (cat. C15410241) diluted 1:500 (left). The right picture shows costaining with the cytoskeleton marker Phalloidin.

  • Target Description

    NFYB (UniProt/Swiss-Prot entry P25208) is one of the 3 subunits of the ubiquitous transcription factor NFY. All three subunits A, B and C are required to form a NFY-DNA complex. There is a connection between mutant p53 gain of function, NF-Y transactivation and DNA damage.

  •  Applications
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
    Immunoprecipitation Read more
  •  Documents
    Datasheet NFYB C15410241 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Publications

    How to properly cite this product in your work

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