Bisulfite modification of DNA is the most commonly used, "gold standard" method for DNA methylation studies providing single nucleotide resolution. This technology is based on the chemical conversion of unmethylated cytosine to uracil. Methylated cytosines are protected from this conversion allowing to determine DNA methylation at the singe nucleotide level. Various analyses can be performed on the altered sequence to retrieve this information: bisulfite sequencing, pyrosequencing, methylation-specific PCR, high resolution melting curve analysis, microarray-based approaches, and next-generation sequencing.
Treatment of DNA with bisulfite converts cytosine residues to uracil, but leaves 5-methylcytosine residues unaffected (see Figures).
| Cat. No. | Product | Format | Price | |
|---|---|---|---|---|
| C02030030 |
Premium Bisulfite kit Make your Bisulfite conversion now in only 90 minutes ! Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion r... |
50 rxns | $210.00 | |
| C02030032 |
Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level with... |
24 rxns | $1,530.00 | |
| C02030033 |
Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit Reduced Representation Bisulfite Sequencing provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level with... |
96 rxns | $4,095.00 | |
| C02030034 |
Premium Whole Genome Bisulfite Sequencing (WGBS) kit Genome-wide screening for DNA methylation - Library Preparation of bisulfite-converted DNA from whole genomes Compatible with ChIP-Bis-Seq - Com... |
8 rxns | $780.00 | |
| C02030031 |
Auto Premium Bisulfite kit Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate... |
40 rxns | $210.00 | |
| C02030035 |
Bisulfite conversion reagent for RRBS This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S... |
8 rxns | $105.00 | |
| C09010010 |
MethylTaq DNA polymerase MethylTaq DNA polymerase is a high-performance Hot Start thermostable recombinant DNA polymerase. MethylTaq is an extremely robust modified Taq DNA polymeras... |
250 units | $230.00 | |
| C05010033 |
Premium WGBS Indexes - 12 Theses 12 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite... |
96 rxns | $1,175.00 | |
| C05010034 |
Premium WGBS Indexes - 24 Theses 24 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite... |
192 rxns | $2,115.00 | |
| C05010032 |
Premium WGBS Indexes - 6 Theses 6 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite ... |
48 rxns | $690.00 |
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