DNA Methylation

Sodium bisulfite conversion of genomic DNA is the most commonly used method for DNA methylation studies providing single nucleotide resolution. It enables to differentiate and detect unmethylated versus methylated cytosines. This procedure can then be followed either by PCR amplification or next generation sequencing to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.

How it works

Treatment of DNA with sodium bisulfite converts unmethylated cytosine to uracil, while methylated cytosines remain unchanged. The DNA is then amplified by PCR where the uracils are converted to thymines.

Advantages

Single nucleotide resolution
Gene-specific and genome-wide analyses
NGS compatible

Downstream analysis techniques

Whole Genome Bisulfite Sequencing (WGBS) with our Premium WGBS Kit
Reduced Representation Bisulfite Sequencing (RRBS) with our Premium RRBS Kit V2
Bisulfite conversion with our Premium Bisulfite Kit followed by qPCR, Sanger, Pyrosequencing

Products

Cat. No.	Product	Format	Price
C02030030	Premium Bisulfite kit Make your Bisulfite conversion now in only 90 minutes ! Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion r...	50 rxns	$214.00
C02030032	Premium RRBS kit Reduced Representation Bisulfite Sequencing (RRBS) provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level&nb...	24 rxns	$1,561.00
C02030033	Premium RRBS kit Reduced Representation Bisulfite Sequencing (RRBS) provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level&nb...	96 rxns	$4,177.00
C02030036	Premium RRBS kit V2 RRBS for low DNA amounts and accurate analysis Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, focused solution to perform genome-scale DNA methylation analysis at the sin...	24 rxns	$1,480.00
C02030034	Premium Whole Genome Bisulfite Sequencing (WGBS) kit Genome-wide screening for DNA methylation - Library Preparation of bisulfite-converted DNA from whole genomes Compatible with ChIP-Bis-Seq - Com...	8 rxns	$796.00
C02030031	Auto Premium Bisulfite kit Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...	40 rxns	$214.00
C02030035	Bisulfite conversion reagent for RRBS This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S...	8 rxns	$107.00
C02040019	DNA methylation control package V2 The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer sets that...	48 rxns	$270.00
C09010010	MethylTaq DNA polymerase MethylTaq DNA polymerase is a high-performance Hot Start thermostable recombinant DNA polymerase. MethylTaq is an extremely robust modified Taq DNA polymeras...	250 units	$235.00
C09010012	MethylTaq Plus 2X Master Mix MethylTaq Plus 2X Master Mix is a ready-to-use cocktail containing all components required for the amplification of bisulfite converted NGS libraries, e...	135 μL	$105.00
C02030040	Premium Methyl UDI-UMI Adapters Module - Set A Premium Methyl UDI-UMI Adapters Module - Set A includes 24 methylated full-length adapters with unique dual indexes (UDI) and unique mole...	24 rxns	$235.00
C02030041	Premium Methyl UDI-UMI Adapters Module - Set B (Coming soon) Premium Methyl UDI-UMI Adapters Module - Set B includes 24 methylated full-length adapters with unique dual indexes (UDI) and&n...	24 rxns		Get a quote You are about to request a quote for Premium Methyl UDI-UMI Adapters Module - Set B. Fill out the form below and we will be in touch with you very soon. All * fields are mandatory Contact Information First name ^* Last name ^* Company ^* Phone number City Country ^* State Email ^* Email verification^* Comment ×
C05010033	Premium WGBS Indexes - 12 Theses 12 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...	96 rxns	$1,199.00
C05010034	Premium WGBS Indexes - 24 Theses 24 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...	192 rxns	$2,157.00
C05010032	Premium WGBS Indexes - 6 Theses 6 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite ...	48 rxns	$704.00

Bisulfite conversion

How it works

Advantages

Downstream analysis techniques

Products

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