Diagenode

DNA methylation

Bisulfite conversion


Sodium bisulfite conversion of genomic DNA is the most commonly used method for DNA methylation studies providing single nucleotide resolution. It enables to differentiate and detect unmethylated versus methylated cytosines. This procedure can then be followed either by PCR amplification or next generation sequencing to reveal the methylation status of every cytosine in gene specific amplification or whole genome amplification.

How it works

Treatment of DNA with sodium bisulfite converts unmethylated cytosine to uracil, while methylated cytosines remain unchanged. The DNA is then amplified by PCR where the uracils are converted to thymines. 

Advantages

  •  Single nucleotide resolution
  •  Gene-specific and genome-wide analyses
  •  NGS compatible

Downstream analysis techniques

Products

Cat. No.ProductFormatPrice
C02030030 Premium Bisulfite kit
Make your Bisulfite conversion now in only 90 minutes ! Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion r...
50 rxns $214.00
C02030032 Premium RRBS kit
Reduced Representation Bisulfite Sequencing (RRBS) provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level&nb...
24 rxns $1,561.00
C02030033 Premium RRBS kit
Reduced Representation Bisulfite Sequencing (RRBS) provides a powerful method to efficiently analyze DNA methylation at the single nucleotide level&nb...
96 rxns $4,177.00
C02030036 Premium RRBS kit V2
RRBS for low DNA amounts and accurate analysis

Reduced Representation Bisulfite Sequencing (RRBS) offers a cost-effective, focused solution to perform genome-scale DNA methylation analysis at the sin...
24 rxns $1,480.00
C02030034 Premium Whole Genome Bisulfite Sequencing (WGBS) kit
Genome-wide screening for DNA methylation - Library Preparation of bisulfite-converted DNA from whole genomes Compatible with ChIP-Bis-Seq - Com...
8 rxns $796.00
C02030031 Auto Premium Bisulfite kit
Diagenode's Premium Bisulfite Kit rapidly converts DNA through bisulfite treatment. Our conversion reagent is added directly to DNA, requires no intermediate...
40 rxns $214.00
C02030035 Bisulfite conversion reagent for RRBS
This reagent is compatible with the Premium RRBS Kit and provides reagents for additional bisulfite conversion reactions. Reduced Representation Bisulfite S...
8 rxns $107.00
C02040019 DNA methylation control package V2
The DNA methylation control package V2 includes one methylated and one unmethylated spike-in controls together with their corresponding qPCR primer sets that...
48 rxns $270.00
C09010010 MethylTaq DNA polymerase
MethylTaq DNA polymerase is a high-performance Hot Start thermostable recombinant DNA polymerase. MethylTaq is an extremely robust modified Taq DNA polymeras...
250 units $235.00
C09010012 MethylTaq Plus 2X Master Mix
MethylTaq Plus 2X Master Mix is a ready-to-use cocktail containing all components required for the amplification of bisulfite converted NGS libraries, e...
135 μL $105.00
C02030040 Premium Methyl UDI-UMI Adapters Module - Set A
Premium Methyl UDI-UMI Adapters Module - Set A includes 24 methylated full-length adapters with unique dual indexes (UDI) and unique mole...
24 rxns $235.00
C02030041 Premium Methyl UDI-UMI Adapters Module - Set B
(Coming soon) Premium Methyl UDI-UMI Adapters Module - Set B includes 24 methylated full-length adapters with unique dual indexes (UDI) and&n...
24 rxns
C05010033 Premium WGBS Indexes - 12
Theses 12 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
96 rxns $1,199.00
C05010034 Premium WGBS Indexes - 24
Theses 24 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite...
192 rxns $2,157.00
C05010032 Premium WGBS Indexes - 6
Theses 6 different indexes are designed for the Premium Whole Genome Bisulfite Sequencing (WGBS) Kit and allow for preparing single and paired-end bisulfite ...
48 rxns $704.00

Events

  • Virtual ChIL-seq webinar
    Diagenode
    Dec 8, 2021
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