Diagenode

Shearing Technologies

Exceptional shearing and sonication of biological samples for any field of study

Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.

We offer three types of sonication/shearing devices to match your exact needs for your application:

  • The Bioruptor® uses a highly-controlled ultrasonic energy with ACT (Adaptive Cavitation Technology), a non-contact, parallel-processing and isothermal technology, to achieve optimal fragment sizes between 150bp-2kb which is ideal for chromatin shearing for chromatin immunoprecipitation (ChIP), genomic DNA shearing for next generation sequencing, and RNA shearing. In addition, the Bioruptor is perfect for cell and tissue disruption, protein processing for mass spectrometry, and much more. The Bioruptor is available in two models: the Bioruptor Plus, specifically designed for protein processing for mass spectrometry, high volume cell and tissue shearing, ChIP-qPCR, and other applications and the Bioruptor Pico for ChIP-seq and NGS.
  • The One® is a unique desktop sonication device perfect for optimal small volume DNA shearing for Next-Generation-Sequencing in the 150bp - 1kb range.
  • The Megaruptor® is a hydrodynamic shearing instrument for DNA shearing between 3kb - 75kb and represents the best shearing device for the tightest distribution of long DNA fragments for applications such as long fragment sequencing.

Bioruptor®

Megaruptor®

One®

FEATURES

  • All in one shearing device
  • Advanced temperature control
  • > 2000 peer reviewed publications
  • Optimal for long DNA fragment generation
  • Tight fragment length distribution
  • Hydropores eliminate clogging issue
  • Compact size
  • Small volume DNA and Chromatin shearing
  • Fully integrated system

APPLICATIONS

  • DNA, Chromatin and RNA shearing 150bp to 2kb
  • FFPE DNA and RNA extraction
  • Protein extraction from cells and tissues
  • Chemical applications (liposomes, emulsifications etc.)
  • Mass spectromy - protein separation
  • DNA shearing 2kb to 75kb
  • DNA and Chromatin shearing 150bp to 1kb

Fields of studies where great sonication and shearing make a difference

Neuroscience and
neurological disease
Cancer research
Environment and toxicology
Stem cell development
Plants and agriculture

Popular applications

  • Protein solubilization
  • DNA extraction from DNA or RNA from cells and tissues
  • Mass spectrometry - protein separation
  • Chromatin shearing for ChIP-seq or ChIP-qPCR
  • Next Generation Sequencing:DNA, RNA, and DNA methylation
  • Protein extraction
  • RNA extraction
  • Liposomes
  • Emulsification

Diagenode has partnered with leading innovative companies to optimize workflows through the greater incorporation of Bioruptor® technology


MS-BASED PROTEOMICS TECHNOLOGIES

Read our partnership application note for mass spectrometry applications

Visit partner website


DNA SHEARING AND SIZING FOR NGS

Read our partnership application note for using fragment analysis after sonication

Visit partner website


LONG FRAGMENT DNA SEQUENCING

Read our partnership application note for optimal long fragment sequencing

Visit partner website

Bioruptor®


  • Bioruptor-pico-next-gen-sequencing
    B01060010
    Bioruptor® Pico sonication device
  • some alt
    B01020001
    Bioruptor® Plus sonication device

Megaruptor®


  • Megaruptor 2
    B06010002
    Megaruptor® 2

One®


  • Bioruptor One
    B01070001
    Diagenode One sonication device

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