Diagenode

NCOR1 polyclonal antibody

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15410341
100 μl
$305.00
  Bulk order

Other names: NCoR, TRAC1, PPP1R109

Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using a synthetic peptide containing a sequence from the central part of the protein1.

LotA301-145A2
Concentration0.2 μg/μl
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal
PurityAffinity purified polyclonal antibody in TBS containing 0.1% BSA and 0.09% azide.
HostRabbit
Storage ConditionsStore at 4°C.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 2 μg/ChIP Fig 1, 2
Western Blotting 1:5,000 Fig 3
IP 6 μg per IP Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μg per IP.

1Manufactured by Bethyl Laboratories, Inc., Texas, USA

  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1
    ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    ChIP-seq A

    ChIP-seq B

    ChIP-seq C

    ChIP-seq D

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1
    ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).

    WB

    Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1
    Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    IP

    Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1
    Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 μg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.

  • Applications
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IP
    Immunoprecipitation Read more
  • Documents
    NCOR1 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using ...
    Download
  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: NCOR1 polyclonal antibody (Diagenode Cat# C15410341 Lot# A301-145A2). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

Events

  • The 12th Annual Meeting of the Japanese Society for Epigenetics
    Jun 24-Jun 25, 2018
 See all events

Applications

 See all applications



The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.



  ABOUT SSL CERTIFICATES

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics