NCOR1 Antibody (sample size)

Catalog Number
20 µl
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Other names: NCoR, TRAC1, PPP1R109

Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using a synthetic peptide containing a sequence from the central part of the protein1.

Concentration0.2 μg/μl
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal, ChIP grade, ChIP-seq grade
PurityAffinity purified polyclonal antibody.
Storage ConditionsStore at 4°C.
Storage BufferTBS containing 0.1% BSA and 0.09% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 2 μg/ChIP Fig 1, 2
Western Blotting 1:5,000 Fig 3
IP 6 μg per IP Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 µg per IP.

1Manufactured by Bethyl Laboratories, Inc., Texas, USA

  • Validation data

    NCOR1 Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against NCOR1
    ChIP assays were performed using K562 cells, the Diagenode antibody against NCOR1 (Cat. No. C15410341) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2 and 5 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the TGM2 gene, used as positive control, and for the MYOD1 gene and Sat2 satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    A.NCOR1 Antibody ChIP-seq Grade

    B.NCOR1 Antibody for ChIP-seq

    C.NCOR1 Antibody for ChIP-seq assay

    D.NCOR1 Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against NCOR1
    ChIP was performed on sheared chromatin from 4 million K562 cells using 2 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341) as described above. The IP’d DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 600 Kb region of the human X-chromosome (fig 2A and B), and in two genomic regions surrounding the TGM2 positive control genes on chromosome 20 (fig 2C) and the EZH1 gene on chromosome 17 (fig 2D).

    NCOR1 Antibody validated in Western Blot

    Figure 3. Western blot analysis using the Diagenode antibody directed against NCOR1
    Whole cell extracts from HeLa (lane 1) and 3T3 cells (lane 2) were analysed by Western blot using the Diagenode antibody against NCOR1 (Cat. No. C15410341) diluted 1:5,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    NCOR1 Antibody validated in Immunoprecipitation

    Figure 4. Immunoprecipitation using the Diagenode antibody directed against NCOR1
    Immunoprecipitation was performed on whole cell extracts from HeLa cells using 6 µg of the Diagenode antibody against NCOR1 (Cat. No. C15410341, lane 1). An equal amount of rabbit IgG was used as a negative control (lane 2). The immunoprecipitated NCOR1 protein was detected by western blot with the NCOR1 antibody diluted 1:200.

  • Target Description

    NCOR1 (UniProtKB/Swiss-Prot entry O75376) mediates transcriptional repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. NCOR1 is part of a complex which may impede the access of basal transcription factors.by promoting histone deacetylation and the formation of repressive chromatin structures.

  •  Applications
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunoprecipitation Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
  •  Documents
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    NCOR1 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human NCOR1 (Nuclear Receptor Corepressor 1), using ...
  •  Safety sheets
    NCOR1 Antibody SDS US en Download
    NCOR1 Antibody SDS GB en Download
    NCOR1 Antibody SDS ES es Download
    NCOR1 Antibody SDS DE de Download
    NCOR1 Antibody SDS JP ja Download
    NCOR1 Antibody SDS BE nl Download
    NCOR1 Antibody SDS BE fr Download
    NCOR1 Antibody SDS FR fr Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: NCOR1 Antibody (sample size) (Diagenode Cat# C15410341-20 Lot# A301-145A3). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Estrone, the major postmenopausal estrogen, binds ERa to induceSNAI2, epithelial-to-mesenchymal transition, and ER+ breast cancermetastasis.
    Qureshi R. et al.
    Recent work showed that the dominant post-menopausal estrogen, estrone, cooperates with nuclear factor κB (NF-κB) to stimulate inflammation, while pre-menopausal 17β-estradiol opposes NF-κB. Here, we show that post-menopausal estrone, but not 17β-estradiol, activates epithelial-to-mesench...


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