Diagenode

H4K20ac monoclonal antibody

Histone-Deacetylase-polyclonal-antibody-diagenode
Catalog Number
Format
Price
C15210008
100 µg/100 µl
$305.00
  Bulk order

Monoclonal antibody raised in rabbit against histone H4 acetylated at Lys20 (H4K20ac), using a KLH-conjugated synthetic peptide.

Lot001
Concentration1 μg/μl
Species reactivityHuman. Other species: not tested.
TypeMonoclonal
PurityProtein A purified monoclonal antibody in PBS containing 50% glycerol, 1% BSA and 0.09% azide.
HostRabbit. Other species: not tested.
Storage ConditionsStore at -20°C
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP * 0.5 - 1 μg/ChIP Fig 1, 2
Western Blotting 1:1,000 Fig 3
Immunofluorescence 1:500 Fig 4

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 0.5-5 μg per IP.

  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode monoclonal antibody directed against H4K20ac
    ChIP assays were performed using human HeLa cells, the Diagenode antibody against H4K20ac (cat. No. C15210008) and optimized PCR primer sets for qPCR. ChIP was performed with the "iDeal ChIP-seq" kit (cat. No. C01010055) on sheared chromatin from 1,000,000 cells. A titration of the antibody consisting of 0.5, 1, 2 and 5 µg per ChIP experiment was analysed. IgG (1 µg/IP) was used as negative IP control. QPCR was performed with primers for the active EIF4A2 and GAPDHgenes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    A.
    ChIP-seq

    B.
    ChIP-seq

    C.
    ChIP-seq

    D.
    ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against H4K20ac
    ChIP was performed with 0.5 µg of the Diagenode antibody against H4K20ac (cat. No. C15210008) on sheared chromatin from 1,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 2000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 600 kb region of the human X chromosome (figure 2A and B) and in two genomic regions surrounding the EIF4A2 and GAPDH positive control genes (figure 2C and D).

    WB

    Figure 3. Western blot analysis using the Diagenode monoclonal antibody directed against H4K20ac
    Histone extracts from HeLa cells treated with butyrate (lane 1) or untreated control cells (lane 2) were analysed by Western blot using the Diagenode monoclonal antibody against H4K20ac (cat. No. C15210008) diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

    IF

    Figure 4. Immunofluorescence using the Diagenode monoclonal antibody directed against H4K20ac
    HeLa cells treated with sodium butyrate were stained with the Diagenode antibody against H4K20ac No. C15210008, red) diluted 1:500. Actin filaments were stained with fluorescein phalladoin (green).

  • Applications
    ChIP-seq (ab)
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    ChIP-qPCR (ab)
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    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    IF
    Immunofluorescence: Diagenode offers huge selection of highly sensitive antibodies validated in IF. Immunofluorescence using the Diagenode monoclonal antibody directed against CRISPR/Cas9 HeLa cells transfected with a Cas9 expression vector (... Read more
  • Documents
    H4K20ac monoclonal antibody DATASHEET
    Monoclonal antibody raised in rabbit against histone H4 acetylated at Lys20 (H4K20ac), using a KL...
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  • Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: H4K20ac monoclonal antibody (Diagenode Cat# C15210008 Lot# 001). Click here to copy to clipboard.

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