Diagenode

Epigenetics Chromatin Analysis

ChIP-seq

Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomic researches, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.
The ChIP-seq workflow


  1. Chromatin preparation: Crosslink chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.
  2. Chromatin shearing: Fragment chromatin by sonication to desired fragment size (100-500 bp)
  3. Chromatin IP: Capture protein-DNA complexes with specific ChIP-seq grade antibodies against the histone or transcription factor of interest
  4. DNA purification: Reverse cross-links, elute, and purify 
  5. NGS Library Preparation: Ligate adapters and amplify IP'd material
  6. Bioinformatic analysis: Perform read filtering and trimming, read specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc.

Need guidance?

Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.

Made for ChIP-seq

Cat. No.ProductFormatPrice
C01010171 Auto iDeal ChIP-seq kit for Histones
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ...
100 rxns VAR pricing available
C01010172 Auto iDeal ChIP-seq Kit for Transcription Factors
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. U...
100 rxns VAR pricing available
C01010140 Auto True MicroChIP kit
The Auto True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This m...
16 rxns VAR pricing available
C01010153 Auto Universal Plant ChIP-seq kit
The Auto Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, ma...
24 rxns VAR pricing available
C01019027 ChIP Cross-link Gold
Cross-linking is typically achieved by using formaldehyde which forms reversible DNA-protein links.However, formaldehyde is usually not effective to cross-li...
600 µl VAR pricing available
G02010000 ChIP-seq Profiling service
ChIP-seq at your fingertips: Full service from A to Z Utilize our expertise: 10 years of experience in ChIP-Seq and official partner of IHEC-BLUEPRI...
C01010051 iDeal ChIP-seq kit for Histones
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ChIP prior...
24 rxns VAR pricing available
C01010055 iDeal ChIP-seq kit for Transcription Factors
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. U...
24 rxns VAR pricing available
B03000002 IP-Star® Compact Automated System
The IP-Star® Compact Automated System provides the flexibility needed to change multiple parameters in the protocols to achieve optimal experimental cond...
1 unit
B01060001 Bioruptor® Pico sonication device
The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system optimized for shearing micro-...
1 unit
C01010130 True MicroChIP Kit
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. T...
16 rxns VAR pricing available
C01010152 Universal Plant ChIP-seq kit
The Universal Plant ChIP-seq kit offers the convenience of extracting plant chromatin from a wide variety of plants including  Arabidopsis, maize, ...
24 rxns VAR pricing available
C30010014 2 ml microtubes for SX-8G IP-Star® Compact
These tubes are intended for being used on the SX-8G IP-Star® Compact Automated System.
100 pc VAR pricing available
C30080030 96 well microplates for IP-Star®
Standard 96 well transparent plates made by polyestyrene and compatible with automated systems. Solid U-bottom plates free of DNAase and RNAase and human DNA.
10 pc VAR pricing available
C03010010 Auto IPure kit v2
The DNA recovery after ChIP or MeDIP might be an issue for specific downstream applications (e.g. Next generation sequencing). Diagenode's IPure kit is the o...
100 rxns VAR pricing available
C02020011 Auto MethylCap kit
The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methyl binding domain) protein . The Diagenode’s Auto MethylCa...
48 rxns VAR pricing available
C01010141 Auto True MicroChIP & MicroPlex Library Preparation™ Package
The Auto True MicroChIP kit in combination with the MicroPlex Library Preparation kit allows for performing ChIP-seq on as few as 10,000 cells. Thi...
16 ChIP rxns and 12 library prep rxns VAR pricing available
C15410337 BRD4 polyclonal antibody
Polyclonal antibody raised in rabbit against human BRD4 (Bromodomain Containing 4), using two KLH-conjugated synthetic peptides from the N-terminal and centr...
50 µg/20 µl VAR pricing available
C01010053 iDeal ChIP-seq for Histones & Library Preparation Package (incl. Index Primer Set 1)
Don’t risk wasting your precious sequencing samples. Diagenode’s validated iDeal ChIP-seq kit has everything you need for a successful ChIP prior...
24 rxns VAR pricing available
C01010056 iDeal ChIP-seq for Transcription Factors & MicroPlex Library Preparation™ Package
Diagenode’s iDeal ChIP-seq Kit for Transcription Factors is a highly specialized solution for robust Transcription Factor ChIP-seq results. Unlike comp...
10 ChIP and 12 library prep rxns VAR pricing available
C01010173 Immunoprecipitation Buffers (iDeal ChIP-seq kit for Histones)
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker).
24 rxns VAR pricing available
C01010174 Immunoprecipitation Buffers (iDeal ChIP-seq kit for TFs)
This set of immunoprecipitation buffers includes all you need for your IP: ChIP, wash and elution buffers as well as BSA (blocker).
24 rxns VAR pricing available
C03010015 IPure kit v2
High DNA recovery after ChIP or MeDIP is critical for specific downstream applications (e.g. Next generation sequencing). Diagenode's IPure kit is the only D...
100 rxns VAR pricing available
C17040004 Maize B73 inner stem ZmB1-UTR primer pair
This primer pair specifically amplifies a genomic region in the 5’-UTR of the B1 gene from Maize (Zea mays clone B73). The primers are thoroughly teste...
50 µl VAR pricing available
C17040005 Maize B73 inner stem ZmCopia primer pair
This primer pair specifically amplifies a genomic region in the Copia gene from Maize (Zea mays clone B73). The primers are thoroughly tested in conjunction ...
50 µl VAR pricing available
C17040011 Poplar xylem PtrCopia-orth primer pair
This primer pair specifically amplifies a genomic region from a sequence orthologous to the Maize Copia gene from poplar (Populus trichocarpa clone Nisqually...
50 µl VAR pricing available
C17040010 Poplar xylem PtrMYBTF1 primer pair
This primer pair specifically amplifies a genomic region from a gene coding a MYB transcription factor in poplar (Populus trichocarpa clone Nisqually). The p...
50 µl VAR pricing available
C17040009 Rice seedlings OsMADS6 primer pair
This primer pair specifically amplifies a genomic region from the MADS6 gene from rice (Oriza sativa ssp Japonica cv. Nipponbare). The primers are thoroughly...
50 µl VAR pricing available
C17040006 Tomato leaves SlChr2-reg8 primer pair
This primer pair specifically amplifies a genomic region from chromosome 2 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ...
50 µl VAR pricing available
C17040007 Tomato leaves SlChr4-NC1 primer pair
This primer pair specifically amplifies a genomic region from chromosome 4 from tomato (Solanum lycopersicum cv MicroTom). The primers are thoroughly tested ...
50 µl VAR pricing available
C01010131 True MicroChIP & MicroPlex Library Preparation™ Package
The True MicroChIP kit in combination with the MicroPlex Library Preparation™ kit allows for performing ChIP-seq on as few as 10,000 cells. This microC...
16 ChIP rxns and 12 library prep rxns VAR pricing available

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