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SUV39H1 Antibody - ChIP-seq grade

Catalog Number
Format
Price
C15410368
50 µg
$325.00
  Bulk order
Other format



Other names: KMT1A, MG44

Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.

LotA2892-0011
Concentration2.3 µg/µl
Species reactivityHuman: positive
TypePolyclonal ChIP-seq Grade
PurityAffinity purified polyclonal antibody
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq * 2 - 5 µg per ChIP Fig 1, 2
ELISA 1:10,000 - 1:100,000 Fig 3
Western Blotting 1:500 Fig 4

*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1 - 5 µg per IP.

  • Validation data

    Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1
    ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

    A.

    B.

    C.

    D.

    E.

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1
    ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.

    Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1
    Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

  •  Applications
    ChIP-seq
    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on ... Read more
    ELISA
    Enzyme-linked immunosorbent assay. Read more
    WB
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
  •  Documents
    SUV39H1 polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog...
    Download
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: SUV39H1 Antibody - ChIP-seq grade (Diagenode Cat# C15410368 Lot# A2892-0011). Click here to copy to clipboard.

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