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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2a-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
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<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
<p>E. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2e-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human <strong>SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1),</strong> using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>',
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2a-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
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<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
<p>E. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2e-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig4-wb.jpg" alt="SUV39H1 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<div class="large-12 columns">Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</div>
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<h5 class="large-12 columns"><strong></strong></h5>
<h5 class="large-12 columns"><strong>The ChIP-seq workflow</strong></h5>
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<div class="large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>Crosslink chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing:</strong> Fragment chromatin by sonication to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: Capture protein-DNA complexes with <strong><a href="../categories/chip-seq-grade-antibodies">specific ChIP-seq grade antibodies</a></strong> against the histone or transcription factor of interest</li>
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<li class="large-12 columns"><strong>NGS Library Preparation</strong>: Ligate adapters and amplify IP'd material</li>
<li class="large-12 columns"><strong>Bioinformatic analysis</strong>: Perform r<span style="font-weight: 400;">ead filtering and trimming</span>, r<span style="font-weight: 400;">ead specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc. </span></li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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'description' => '<p><strong>Other names: </strong><span lang="EN-GB">KMT1A, MG44</span></p><p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>',
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
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<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2a-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
<div class="extra-spaced"></div>
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<div class="extra-spaced"></div>
<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
<p>E. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2e-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig4-wb.jpg" alt="SUV39H1 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 - 5 µg per ChIP</td>
<td>Fig 1, 2</td>
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<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1 - 5 µg per IP.</small></p>',
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<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human <strong>SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1),</strong> using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>',
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig1-chip.png" alt="SUV39H1 Antibody ChIP Grade" /></center></div>
<div class="small-6 columns">
<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="extra-spaced"></div>
<div class="row">
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2a-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
<p>E. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2e-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
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<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig4-wb.jpg" alt="SUV39H1 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<h5 class="large-12 columns"><strong></strong></h5>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
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<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
<p>E. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2e-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig4-wb.jpg" alt="SUV39H1 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<div class="large-12 columns">Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</div>
<div class="large-12 columns"></div>
<h5 class="large-12 columns"><strong></strong></h5>
<h5 class="large-12 columns"><strong>The ChIP-seq workflow</strong></h5>
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<div class="large-12 columns"><br />
<ol>
<li class="large-12 columns"><strong>Chromatin preparation: </strong>Crosslink chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.</li>
<li class="large-12 columns"><strong>Chromatin shearing:</strong> Fragment chromatin by sonication to desired fragment size (100-500 bp)</li>
<li class="large-12 columns"><strong>Chromatin IP</strong>: Capture protein-DNA complexes with <strong><a href="../categories/chip-seq-grade-antibodies">specific ChIP-seq grade antibodies</a></strong> against the histone or transcription factor of interest</li>
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<li class="large-12 columns"><strong>NGS Library Preparation</strong>: Ligate adapters and amplify IP'd material</li>
<li class="large-12 columns"><strong>Bioinformatic analysis</strong>: Perform r<span style="font-weight: 400;">ead filtering and trimming</span>, r<span style="font-weight: 400;">ead specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc. </span></li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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'description' => '<p><strong>Other names: </strong><span lang="EN-GB">KMT1A, MG44</span></p><p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>',
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
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<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2a-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
<div class="extra-spaced"></div>
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<div class="extra-spaced"></div>
<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
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<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig4-wb.jpg" alt="SUV39H1 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<td>ChIP/ChIP-seq <sup>*</sup></td>
<td>2 - 5 µg per ChIP</td>
<td>Fig 1, 2</td>
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<p><small>*Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1 - 5 µg per IP.</small></p>',
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<p><span lang="EN-GB">Polyclonal antibody raised in rabbit against human <strong>SUV39H1 (Suppressor Of Variegation 3-9 Homolog 1),</strong> using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.</span></p>',
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig1-chip.png" alt="SUV39H1 Antibody ChIP Grade" /></center></div>
<div class="small-6 columns">
<p><strong>Figure 1. ChIP results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP assays were performed using HeLa cells, the Diagenode antibody against SUV39H1 (cat. No. C15410368) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the ADCY5 and EPHA10 genes, as well as a chromosome 2 intergenic region, used as positive controls, and for the MYOD1 gene, used as a negative control. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).</p>
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<div class="extra-spaced"></div>
<div class="row">
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<p>A. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2a-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
<p>B. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2b-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq" /></p>
<p>C. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2c-chip-seq.jpg" alt="SUV39H1 Antibody for Chip-seq assay" /></p>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<div class="extra-spaced"></div>
<p>D. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2d-chip-seq.jpg" alt="SUV39H1 Antibody validated in Chip-seq " /></p>
<p>E. <img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig2e-chip-seq.jpg" alt="SUV39H1 Antibody Chip-seq Grade " /></p>
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<div class="row">
<div class="small-12 columns">
<p><strong>Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against SUV39H1</strong><br />ChIP was performed with 5 µg of the Diagenode antibody against SUV39H1 (cat. No. C15410368) on sheared chromatin from 4,000,000 HeLa cells using the “iDeal ChIP-seq” kit as described above. The IP'd DNA was subsequently analysed on an Illumina NovaSeq. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the signal distribution along the complete sequence and a 3 Mb region of human chromosome 1, (figures 2A and B), in two genomic regions surrounding the ADCY5 and EPHA10 positive control genes (figure 2C and D) and in an intergenic region of chromosome 2 (figure 2E).</p>
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<div class="small-6 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig3-elisa.png" alt="SUV39H1 Antibody ELISA Validation " /></center></div>
<div class="small-6 columns">
<p><strong>Figure 3. Determination of the antibody titer</strong><br />To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against SUV39H1 (cat. No. C15410368). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be >1:1,000,000.</p>
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<div class="small-4 columns"><center><img src="https://www.diagenode.com/img/product/antibodies/c15410368-fig4-wb.jpg" alt="SUV39H1 Antibody validated in Western Blot" /></center></div>
<div class="small-8 columns">
<p><strong>Figure 4. Western blot analysis using the Diagenode antibody directed against SUV39H1</strong><br />Whole cell extracts from HeLa cells were analysed by Western blot using the Diagenode antibody against SUV39H1 (cat. No. C15410368) diluted 1:500 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.</p>
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<h5 class="large-12 columns"><strong></strong></h5>
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<li class="large-12 columns"><strong>NGS Library Preparation</strong>: Ligate adapters and amplify IP'd material</li>
<li class="large-12 columns"><strong>Bioinformatic analysis</strong>: Perform r<span style="font-weight: 400;">ead filtering and trimming</span>, r<span style="font-weight: 400;">ead specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc. </span></li>
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<h3 class="text-center" style="color: #b21329;">Need guidance?</h3>
<p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p>
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<p>Learn more about: <a href="https://www.diagenode.com/applications/western-blot">Loading control, MW marker visualization</a><em>. <br /></em></p>
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<p><span style="font-weight: 400;">Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.</span></p>
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<p><strong>ChIP-seq profile</strong> of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.</p>
<img src="https://www.diagenode.com/img/categories/antibodies/chip-seq-grade-antibodies.png" /></div>
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<p><small> ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region. </small></p>
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<p><strong>ChIP results</strong> obtained with the antibody directed against H3K4me3 (Cat. No. <a href="../p/h3k4me3-polyclonal-antibody-premium-50-ug-50-ul">C15410003</a>). </p>
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include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
×