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High performance of Diagenode’s antibodies in CUT&Tag

High Quality Antibodies

For over 15 years Diagenode has been the most trusted supplier for epigenetics-focused antibodies. We understand that high quality is crucial for the success of experiments, so we emphasize strict quality standards and the actual validation for all of our antibodies in applications such ChIP and ChIP-seq. Our antibodies are produced and validated following the ChIP-seq guidelines defined by major epigenetic consortia including Blueprint and ENCODE. Recently our standard antibody QC has been enriched by CUT&Tag validation enabling to select the antibodies with high performance in this assay.

Why Diagenode:

  • We perform ChIP-seq and CUT&Tag validation in-house following strict QC criteria
  • We assure ChIP-seq and CUT&Tag validation on every lot
  • We confirm the specificity of the antibody using peptide array or siRNA
  • We guarantee the performance of our antibodies for the tested applications
  • We are transparent - validation data is available on our website

A.

Figure 1. Representative screenshot for H3K4me3 (A and B), H3K27me3 (C and D), H3K9me3 (E and F) data obtained using iDeal CUT&Tag kit for Histones (Cat. No. C01070020) and Antibody package for Histones anti-rabbit (Cat. No. C01070022) at selected loci. CUT&Tag was performed using 50,000 K562 cells and Diagenode H3K4me3 polyclonal CUT&Tag antibody (Cat. No. C15410003), H3K27me3 polyclonal CUT&Tag grade antibody (Cat. No. C15410195) and H3K9me3 poyclonal CUT&Tag grade antibody (Cat. No. C15410193).

A.

B.

Figure 2. Typical library profile generated by CUT&Tag protocol. Agilent BioAnalyzer trace of pooled libraries derived from H3K4me3, H3K27me3 and H3K9me3 CUT&Tag experiments performed using 50,000 K562 cells (A) and from H3K27me3 and H3K9me3 CUT&Tag experiments using 25 000, 10 000 and 1 000 K562 cells (B). The following Diagenode antibodies were used: H3K4me3 polyclonal CUT&Tag antibody (Cat. No. C15410003), H3K27me3 polyclonal CUT&Tag grade antibody (Cat. No. C15410195) and H3K9me3 polyclonal CUT&Tag grade antibody (Cat. No. C15410193).

活动

  • London Calling 2024
    London, UK
    May 21-May 24, 2024
  • Symposium of the Young Scientist Association
    Vienna, Austria
    May 28-May 29, 2024
  • ESHG 2024
    Berlin, Germany
    Jun 1-Jun 4, 2024
  • CLEPIC 2024
    Warsaw, Poland
    Jun 5-Jun 7, 2024
  • EACR 2024
    Rotterdam, Netherlands
    Jun 10-Jun 13, 2024
  • Chromatin meets South 2024
    Marseille, France
    Jun 13-Jun 14, 2024
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