Diagenode

Auto MethylCap kit

Catalog Number
Format
Price
C02020011
(AF-Auto01-0048)
48 rxns
$510.00

The MethylCap assay is based on the affinity purification of methylated DNA using a MBD (Methyl binding domain) protein . The Diagenode’s Auto MethylCapTM kit is designed to perform automated immunoprecipitation of Chromatin using the SX-8G IP-Star® Automated System enabling highly reproducible results and allowing for high throughput.

  •  Applications
    ChIP-seq
    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on ... Read more
    Methylbinding domain protein
    The MBD approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containin... Read more
  •  Documents
    Auto MethylCap kit MANUAL
    This H6-GST-MBD fusion protein (cat# mbd-001-100) has been extensively validated. It consis...
    Download
    Datasheet MethylCap Protein DATASHEET
    This MethylCap protein has been extensively validated for specific isolation of DNA containing me...
    Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Auto MethylCap kit (Diagenode Cat# C02020011). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Gene promoter and exon DNA methylation changes in colon cancer development - mRNA expression and tumor mutation alterations.
    Molnár B, Galamb O, Péterfia B, Wichmann B, Csabai I, Bodor A, Kalmár A, Szigeti KA, Barták BK, Nagy ZB, Valcz G, Patai ÁV, Igaz P, Tulassay Z
    BACKGROUND: DNA mutations occur randomly and sporadically in growth-related genes, mostly on cytosines. Demethylation of cytosines may lead to genetic instability through spontaneous deamination. Aims were whole genome methylation and targeted mutation analysis of colorectal cancer (CRC)-related genes and mRNA expre...

    Aberrant DNA methylation of WNT pathway genes in the development and progression of CIMP-negative colorectal cancer.
    Galamb O et al.
    The WNT signaling pathway has an essential role in colorectal carcinogenesis and progression, which involves a cascade of genetic and epigenetic changes. We aimed to analyze DNA methylation affecting the WNT pathway genes in colorectal carcinogenesis in promoter and gene body regions using whole methylome analysis i...

  •  Related products

         Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics