This H6-GST-MBD fusion protein (cat# mbd-001-100) has been extensively validated. It consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase (GST) containing an N-terminal His6-tag. The H6-GST-MBD fusion protein can be used to specifically isolate DNA containing methylated CpGs. See overview and protocol below.
GST protein (cat# gst-001-050) can also be purchased, to be used as negative control in the MBD pull-down experiment.
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