Diagenode

Epigenetics Chromatin Analysis

Epigenetics research addresses alterations to chromatin that propagate different patterns of transcription or gene expression and silencing.

The major components of chromatin are DNA and histone proteins. Two copies of each histone core protein (H2A, H2B, H3 & H4) are assembled into an octamer, wrapped around by DNA to form a nucleosome core. The nucleosome controls the accessibility of DNA to the transcription machinery and chromatin remodelling factors.
Chromatin immunoprecipitation (ChIP) is a method used to determine the location of genome binding sites for a specific protein of interest, giving invaluable insights into the regulation of gene expression. ChIP involved the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognize a specific protein or protein modification are used to determine the relative abundance of the antigen at specific loci.
ChIP-seq and ChIP-qPCR are techniques that allow the identification of protein-DNA binding sites.

  • ChIP-qPCR

    Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if ...
  • ChIP-seq

    Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the prim...
  • Chromatin shearing

    The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature...

Made for Epigenetics Chromatin Analysis

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    C01010055
    iDeal ChIP-seq kit for Transcription Factors
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    B03000002
    IP-Star® Compact Automated System

Events

  • ASHG 2017
    Orlando, FL
    Oct 17-Oct 21, 2017
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