DO IT YOURSELF The ChIP workflow and what you will need

Step by step workflow

Optimal solution from Diagenode

1. Crosslink to bind proteins to DNA

• ChIP Cross-link Gold

2. Shear DNA

• Bioruptor^® Pico Sonication device
• Shearing optimization reagent

3. Immunoprecipitate with specific antibody

• ChIP-seq and ChIP-qPCR kits for transcription factors, histones, low inputs, plants
• ChIP and ChIP-seq grade antibodies
• Automation available

4. Reverse crosslinks and purify

• Diagenode's ChIP kits (contain optimal purification modules)

5. ChIP-qPCR or ChIP-seq library preparation

• ChIP-seq:

• MicroPlex Library Preparation for 50 pg - 5 ng
• iDeal Library Preparation for > 5 ng

• ChIP qPCR