Diagenode

Antibodies

ChIP-seq grade antibodies

Unparalleled ChIP-Seq results with the most rigorously validated antibodies

Diagenode provides leading solutions for epigenetic research. Because ChIP-seq is a widely-used technique, we validate our antibodies in ChIP and ChIP-seq experiments (in addition to conventional methods like Western blot, Dot blot, ELISA, and immunofluorescence) to provide the highest quality antibody. We standardize our validation and production to guarantee high product quality without technical bias. Diagenode guarantees ChIP-seq grade antibody performance under our suggested conditions.

ChIP-seq profile of active (H3K4me3 and H3K36me3) and inactive (H3K27me3) marks using Diagenode antibodies.

ChIP was performed on sheared chromatin from 100,000 K562 cells using iDeal ChIP-seq kit for Histones (cat. No. C01010051) with 1 µg of the Diagenode antibodies against H3K27me3 (cat. No. C15410195) and H3K4me3 (cat. No. C15410003), and 0.5 µg of the antibody against H3K36me3 (cat. No. C15410192). The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. The figure shows the signal distribution along the complete sequence of human chromosome 3, a zoomin to a 10 Mb region and a further zoomin to a 1.5 Mb region.

Diagenode’s highly validated antibodies:

  • Highly sensitive and specific
  • Cost-effective (requires less antibody per reaction)
  • Batch-specific data is available on the website
  • Expert technical support
  • Sample sizes available
  • 100% satisfaction guarantee

Products

ProductRating
ApplicationPurity
AML1-ETO polyclonal antibody - Classic
(100 µl)
WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab) Whole antiserum
CBFb polyclonal antibody - Classic
(100 µl)
ELISA, ChIP-seq (ab), ChIP-qPCR (ab) Whole antiserum
CBP polyclonal antibody - Classic
(100 μg)
WB, IF, IP, IHC, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
CTCF polyclonal antibody - Classic
(50 μg)
WB, ELISA, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
CXXC1 polyclonal antibody - Classic
(50 μg /50 μl)
WB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
E2F6 polyclonal antibody - Classic
(50 μg /50 μl)
WB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
ER alpha monoclonal antibody - Classic
(100 µl)
WB, ELISA, IP, IC, GSA, ChIP-seq (ab), ChIP-qPCR (ab) Ascites fluid
ETO polyclonal antibody - Classic
(100 µl)
ELISA, ChIP-seq (ab), ChIP-qPCR (ab) Whole antiserum
EZH2 polyclonal antibody - Classic
(50 µg/50 µl)
WB, IC, ChIP-seq (ab), ChIP-qPCR (ab) Protein G purified
FOXA1 polyclonal antibody - Classic
(100 μg)
WB, IF, IP, IHC, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
FOXM1 polyclonal antibody - Classic
(100 μg)
WB, IHC, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
GR monoclonal antibody - Classic
(50 µg/50 µl)
WB, ELISA, IP, GSA, Flow Cyt, ChIP-seq (ab), ChIP-qPCR (ab) Protein A/G purified
H2A.Z polyclonal antibody - Premium
(50 μg/33 μl)
WB, ELISA, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H2A.Zac polyclonal antibody - Classic
(50 μg/72 μl)
ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H2A.Zac polyclonal antibody - Premium
(50 μg/46 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H2AK5ac polyclonal antibody - Classic
(50 μg/ 54 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H2BK12ac polyclonal antibody - Classic
(50 μg/ 61 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H2BK15ac polyclonal antibody - Classic
(50 μg/ 24 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K18ac polyclonal antibody - Classic
(50 μg/62 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K27ac polyclonal antibody - Classic
(50 μg/42 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K27ac polyclonal antibody - Premium
(50 μg/18 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K27me3 polyclonal antibody - Classic
(50 μg/34 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K27me3 polyclonal antibody - Premium
(50 μg/27 μl)
WB, ELISA, DB, IF, Peptide array, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K27me3S28p polyclonal antibody - Classic
(50 μg/ 295 μl)
WB, ELISA, DB, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K36ac polyclonal antibody - Classic
(50 μg)
WB, ELISA, DB, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K36me2 polyclonal antibody - Classic
(100 µl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Whole antiserum
H3K36me3 polyclonal antibody - Classic
(100 µl)
WB, ELISA, DB, ChIP-seq (ab), ChIP-qPCR (ab) Whole antiserum
H3K36me3 polyclonal antibody - Classic
(50 µg/56 µl)
WB, ELISA, DB, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K36me3 polyclonal antibody - Premium
(50 μg/42 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K4ac polyclonal antibody - Classic
(50 μg/46 μl)
WB, ELISA, DB, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K4me1 polyclonal antibody - Classic
(50 µg/18 µl)
WB, ELISA, DB, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K4me1 polyclonal antibody - Premium
(50 μg/54 μl)
WB, ELISA, DB, IF, Peptide array, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K4me2 polyclonal antibody - Classic
(50 μg/42 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K4me3 monoclonal antibody - Classic
(50 µg/50 µl)
WB, ELISA, IF, ChIP-seq (ab), ChIP-qPCR (ab) Protein A/G purified
H3K4me3 polyclonal antibody - Premium
(50 µg/36 µl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K56ac polyclonal antibody - Classic
(50 μg/ 84 μl)
WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K79me1 polyclonal antibody - Classic
(50 μg/31 μl)
WB, ELISA, DB, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K79me2 polyclonal antibody - Classic
(50 μg/46 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K79me3 polyclonal antibody - Classic
(50 µg/42 µl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K9/14ac polyclonal antibody - Classic
(50 μg/36 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K9/14ac polyclonal antibody - Premium
(50 μg/62 μl)
WB, ELISA, DB, IF, Peptide array, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K9ac polyclonal antibody - Classic
(50 µg/37 µl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K9ac polyclonal antibody - Classic
(50 μg/47 μl)
WB, ELISA, DB, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K9me3 polyclonal antibody - Classic
(50 µg/33 µl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K9me3 polyclonal antibody - Premium
(50 μg/19 μl)
WB, ELISA, DB, IF, Peptide array, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H3K9me3 recombinant antibody and negative control - Premium
(50 µg)
IF, ChIP-seq (ab), ChIP-qPCR (ab) affinity purified
H3R17me2(asym)K18ac polyclonal antibody - Classic
(50 μg/53 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H4K20me1 monoclonal antibody - Classic
(50 μg/23 μl)
WB, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Protein A purified
H4K20me3 polyclonal antibody - Classic
(50 μg/47 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H4K20me3 polyclonal antibody - Premium
(50 μg/53 μl)
WB, ELISA, DB, IF, Peptide array, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H4K5,8,12,16ac polyclonal antibody - Classic
(50 μg/66 μl)
DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H4K5,8,12ac polyclonal antibody - Classic
(50 μg/66 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
H4K5ac polyclonal antibody - Classic
(50 μg/28 μl)
WB, ELISA, DB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
HDAC1 polyclonal antibody - Classic
(50 µg/79 µl)
WB, ELISA, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
HDAC2 monoclonal antibody - Classic
(50 μg/ 25 μl)
WB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Protein A purified
KAP1 polyclonal antibody - Classic
(100 μg)
WB, IF, IP, IHC, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
LSD1 polyclonal antibody - Classic
(50 µg/27 µl)
WB, ELISA, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
MYH11 polyclonal antibody - Classic
(100 μl)
WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab) Whole antiserum
NF-E2 polyclonal antibody - Classic
(100 μg)
WB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
NFKB p65 polyclonal antibody - Classic
(100 μl)
WB, ELISA, IHC, ChIP-seq (ab), ChIP-qPCR (ab) Whole antiserum
NFYB polyclonal antibody - Classic
(100 μg)
WB, IF, IP, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
NRF1 monoclonal antibody - Classic
(50 μg/23 μl)
WB, ChIP-qPCR (ab) Protein A purified
OCT4 polylconal antibody - Classic
(50 μg)
WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
p300 monoclonal antibody - Classic
(50 μg)
ChIP-seq (ab), ChIP-qPCR (ab) Protein A purified
p53 polyclonal antibody - Classic
(50 µg/50 µl)
WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
Pol II monoclonal antibody - Classic
(100 µl)
ChIP-seq (ab), ChIP-qPCR (ab) Ascites fluid
Pol II monoclonal antibody - Classic
(50 μg/50 μl)
WB, ELISA, IF, ChIP-seq (ab), ChIP-qPCR (ab) Protein A purified
Pol II S2p monoclonal antibody - Classic
(50 μg/50 μl)
WB, ELISA, IF, ChIP-seq (ab), ChIP-qPCR (ab) Protein A purified
Pol II S5p monoclonal antibody - Classic
(50 μg/50 μl)
WB, ELISA, IF, ChIP-seq (ab), ChIP-qPCR (ab) Protein A purified
RARA polyclonal antibody - Classic
(100 µl)
WB, ELISA, ChIP-seq (ab), ChIP-qPCR (ab) Whole antiserum
RNF2 polyclonal antibody - Classic
(50 μg /50 μl)
WB, IF, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
SAP30 polyclonal antibody - Classic
(50 µg/25 µl)
WB, ChIP-seq (ab), ChIP-qPCR (ab) Protein G purified
SIN3A polyclonal antibody - Classic
(100 μg)
WB, IF, IP, IHC, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
SMAD1 polyclonal antibody - Classic
(100 μg)
WB, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
SMARCB1 polyclonal antibody - Classic
(50 μg /50 μl)
WB, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
TAL1 monoclonal antibody - Classic
(50 μg/50 μl)
ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
TARDBP polyclonal antibody - Classic
(100 μg)
WB, IF, IP, IHC, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified
TBP monoclonal antibody - Classic
(100 µl)
WB, ChIP-seq (ab), ChIP-qPCR (ab) Protein A/G purified
ZHX2 polyclonal antibody - Classic
(100 μg)
WB, IF, IHC, ChIP-seq (ab), ChIP-qPCR (ab) Affinity purified

Events

  • 6th Next Generation Sequencing Conference
    Boston, USA
    May 25-May 27, 2016
  • Shaping life 2016 (IBDM-SFBD)
    Marseille, France
    May 24-May 27, 2016
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