ER alpha monoclonal antibody - Classic

Catalog Number
100 µl
  Bulk order
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Alternative names: ESR, ESR1, ESRA, NR3A1

Monoclonal antibody raised in mouse against human ERalpha (estrogen receptor alpha), using a synthetic peptide.

Concentrationnot determined
Species reactivityHuman, mouse
PurityAscites fluid
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ELISA 1:500 -1:5,000
Western Blotting 1:500 -1:5,000 Ref 3
Gel Supershift 1:10 – 1:20
Immunochemistry 1:500 -1:5,000
Immunoprecipitation 1:200 -1:5,000 Ref 3
ChIP * 2.5 μl/ChIP Fig 1; Ref 1, 2

* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.

  • Validation Data





    Figure 1. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against ERalpha
    ChIP was performed with the Diagenode monoclonal antibody against ERalpha (cat. No. AC-066-100) on sheared chromatin from MCF7 cells treated for 1 hour with estradiol. The IP’d DNA was analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 1 shows the obtained peaks near the TFF1 gene on chromosome 21 (figure 1A), the GREB1 and HAAO genes on chromosome 2 (figure 1B and C), and the ZNF185 gene on the X-chromosome (figure 1D).

  •  Applications
    Enzyme-linked immunosorbent assay. Read more
    Western blot : The quality of antibodies used in this technique is crucial for correct and specific protein identification. Diagenode offers huge selection of highly sensitive and specific western blot-validated antibodies. Learn more about: Load... Read more
    Immunoprecipitation Read more
    Immunochemistry Read more
    ChIP-seq (ab)
    Read more
    ChIP-qPCR (ab)
    Read more
    Gel Supershift Read more
  •  Documents
    Datasheet hERalpha C15100066 DATASHEET
    Datasheet description
    Antibodies you can trust POSTER
    Epigenetic research tools have evolved over time from endpoint PCR to qPCR to the analyses of lar...
    Epigenetic Antibodies Brochure BROCHURE
    More than in any other immuoprecipitation assays, quality antibodies are critical tools in many e...
  •  Publications

    How to properly cite this product in your work

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    Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha.
    Glont SE, Papachristou EK, Sawle A, Holmes KA, Carroll JS, Siersbaek R
    Estrogen Receptor alpha (ERα) plays a major role in most breast cancers, and it is the target of endocrine therapies used in the clinic as standard of care for women with breast cancer expressing this receptor. The two methods ChIP-seq (chromatin immunoprecipitation coupled with deep sequencing) and RIME (Rapi...

    The oestrogen receptor alpha-regulated lncRNA NEAT1 is a critical modulator of prostate cancer.
    Chakravarty D, Sboner A, Nair SS, Giannopoulou E, Li R, Hennig S, Mosquera JM, Pauwels J, Park K, Kossai M, MacDonald TY, Fontugne J, Erho N, Vergara IA, Ghadessi M, Davicioni E, Jenkins RB, Palanisamy N, Chen Z, Nakagawa S, Hirose T, Bander NH, Beltran H
    The androgen receptor (AR) plays a central role in establishing an oncogenic cascade that drives prostate cancer progression. Some prostate cancers escape androgen dependence and are often associated with an aggressive phenotype. The oestrogen receptor alpha (ERα) is expressed in prostate cancers, independent of AR ...

    Estrogen receptor ligands ameliorate fatty liver through a nonclassical estrogen receptor/Liver X receptor pathway in mice.
    Han SI, Komatsu Y, Murayama A, Steffensen KR, Nakagawa Y, Nakajima Y, Suzuki M, Oie S, Parini P, Vedin LL, Kishimoto H, Shimano H, Gustafsson JÅ, Yanagisawa J
    UNLABELLED: Liver X receptor (LXR) activation stimulates triglyceride (TG) accumulation in the liver. Several lines of evidence indicate that estradiol-17β (E2) reduces TG levels in the liver; however, the molecular mechanism underlying the E2 effect remains unclear. Here, we show that administration of E2 attenuate...

    Functional Genomic Methods to Study Estrogen Receptor Activity.
    Gilfillan S, Fiorito E, Hurtado A
    Estrogen Receptor (ER) is a nuclear receptor that mediates the actions of estrogen and tamoxifen. ER is expressed in a major fraction of human breast cancers. Recently, genomic maps for estrogen- and tamoxifen-ER have been published. Interestingly, estrogen and tamoxifen induce similar genomic interactions and both ...

    ChIP-Seq of ERalpha and RNA polymerase II defines genes differentially responding to ligands.
    Welboren WJ, van Driel MA, et al.,
    We used ChIP-Seq to map ERa-binding sites and to profile changes in RNA polymerase II (RNAPII) occupancy in MCF-7 cells in response to estradiol (E2), tamoxifen or fulvestrant. We identify 10 205 high confidence ERa-binding sites in response to E2 of which 68% contain an estrogen response element (ERE) and only 7% c...

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