Alternative names: ESR, ESR1, ESRA, NR3A1
Monoclonal antibody raised in mouse against human ERalpha (estrogen receptor alpha), using a synthetic peptide.
| Lot | 005 |
|---|---|
| Concentration | not determined |
| Species reactivity | Human, mouse |
| Type | Monoclonal |
| Purity | Ascites fluid |
|---|---|
| Host | Mouse |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Applications | Suggested dilution | References |
|---|---|---|
| ELISA | 1:500 -1:5,000 | |
| Western Blotting | 1:500 -1:5,000 | Ref 3 |
| Gel Supershift | 1:10 – 1:20 | |
| Immunochemistry | 1:500 -1:5,000 | |
| Immunoprecipitation | 1:200 -1:5,000 | Ref 3 |
| ChIP * | 2.5 μl/ChIP | Fig 1; Ref 1, 2 |
* Please note that the optimal antibody amount per IP should be determined by the end-user. We recommend testing 1-5 μl per IP.
Figure 1. ChIP-seq results obtained with the Diagenode monoclonal antibody directed against ERalpha
ChIP was performed with the Diagenode monoclonal antibody against ERalpha (cat. No. AC-066-100) on sheared chromatin from MCF7 cells treated for 1 hour with estradiol. The IP’d DNA was analysed with an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 1 shows the obtained peaks near the TFF1 gene on chromosome 21 (figure 1A), the GREB1 and HAAO genes on chromosome 2 (figure 1B and C), and the ZNF185 gene on the X-chromosome (figure 1D).
 How to properly cite this product in your workDiagenode strongly recommends using this: ER alpha monoclonal antibody (sample size) (Diagenode Cat# C15100066-20 Lot# 005). Click here to copy to clipboard. Using our products in your publication? Let us know! |
Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha. |
The oestrogen receptor alpha-regulated lncRNA NEAT1 is a critical modulator of prostate cancer. |
Estrogen receptor ligands ameliorate fatty liver through a nonclassical estrogen receptor/Liver X receptor pathway in mice. |
Functional Genomic Methods to Study Estrogen Receptor Activity. |
ChIP-Seq of ERalpha and RNA polymerase II defines genes differentially responding to ligands. |
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