EHF Antibody

Catalog Number
50 μg
  Bulk order
Other format

Other names: ESE3, ESE-3, ESE3B, ESEJ, HEHF

Polyclonal antibody raised in rabbit against human EHF (ETS Homologous Factor), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.

Concentration0.8 µg/µl
Species reactivityHuman: positive. Other species: not tested.
PurityAffinity purified polyclonal antibody.
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq* 1 – 2 µg per ChIP Fig 1, 2
ELISA 1:1,000 - 1:50,000 Fig 3
Western Blotting Not recommended

*Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 µg per ChIP.

  • Validation data

    EHF Antibody ChIP Grade

    Figure 1. ChIP results obtained with the Diagenode antibody directed against EHF
    ChIP assays were performed using T47D cells, the Diagenode antibody against EHF (Cat. No. C15410363) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ITGA2 and OAS3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls.

    A. EHF Antibody ChIP-seq Grade

    B. EHF Antibody for ChIP-seq

    C. EHF Antibody for ChIP-seq assay

    D. EHF Antibody validated in ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against EHF
    ChIP was performed on sheared chromatin from 4,000,000 T47D cells using 2 µg of the Diagenode antibody against EHF (Cat. No. C15410363) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 300 kb region of chromosome 1 (figure 2A and B) and in two genomic regions surrounding the ITGA2 and OAS3 positive control genes, respectively (figure 2C and D).

    EHF Antibody ELISA Validation

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against EHF (Cat. No. C15410363). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:98,000.

  • Target Description

    EHF (UniProtKB/Swiss-Prot entry Q9NZC4) belongs to the ETS transcription factor family. This transcriptional activator shows epithelial-specific expression and plays a role in regulating epithelial cell differentiation and proliferation. EHF may also act as a transcriptional repressor of a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. EHF is also a putative tumor suppressor gene and may be involved in carcinogenesis.

  •  Applications
  •  Documents
    EHF polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human EHF (ETS Homologous Factor), using two synthet...
  •  Safety sheets
    SDS C15410363 EHF Antibody US en Download
    SDS C15410363 EHF Antibody GB en Download
    SDS C15410363 EHF Antibody BE fr Download
    SDS C15410363 EHF Antibody FR fr Download
    SDS C15410363 EHF Antibody BE nl Download
    SDS C15410363 EHF Antibody DE de Download
    SDS C15410363 EHF Antibody JP ja Download
    SDS C15410363 EHF Antibody ES es Download
  •  Publications

    How to properly cite this product in your work

    Diagenode strongly recommends using this: EHF Antibody (Diagenode Cat# C15410363 Lot# A2883P). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    EHF is a novel regulator of cellular redox metabolism and predictspatient prognosis in HNSCC.
    Oyelakin A. et al.
    Head and Neck Squamous Cell Carcinoma (HNSCC) is a heterogeneous disease with relatively high morbidity and mortality rates. The lack of effective therapies, high recurrence rates and drug resistance driven in part, by tumor heterogeneity, contribute to the poor prognosis for patients diagnosed with this cancer. Thi...


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