Diagenode

EHF polyclonal antibody

カタログ番号
フォーマット
価格
C15410363
50 μg
$315.00
  Bulk order
ブローカー情報



Other names: ESE3, ESE-3, ESE3B, ESEJ, HEHF

Polyclonal antibody raised in rabbit against human EHF (ETS Homologous Factor), using two synthetic peptides containing a sequence from the central part and the C-terminus of the protein, respectively.

LotA2883P
Concentration0.8 µg/µl
Species reactivityHuman: positive. Other species: not tested.
TypePolyclonal
PurityAffinity purified polyclonal antibody.
HostRabbit
Storage ConditionsStore at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Storage BufferPBS containing 0.05% azide.
PrecautionsThis product is for research use only. Not for use in diagnostic or therapeutic procedures.
Applications Suggested dilution References
ChIP/ChIP-seq* 1 – 2 µg per ChIP Fig 1, 2
ELISA 1:1,000 - 1:50,000 Fig 3
Western Blotting Not recommended

*Please note that the optimal antibody amount per ChIP should be determined by the end-user. We recommend testing 1-5 µg per ChIP.

  • Validation data

    ChIP

    Figure 1. ChIP results obtained with the Diagenode antibody directed against EHF
    ChIP assays were performed using T47D cells, the Diagenode antibody against EHF (Cat. No. C15410363) and optimized PCR primer sets for qPCR. ChIP was performed with the “iDeal ChIP-seq” kit (Cat. No. C01010055), using sheared chromatin from 4 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the ITGA2 and OAS3 genes, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat, used as negative controls.

    A. ChIP-seq

    B. ChIP-seq

    C. ChIP-seq

    D. ChIP-seq

    Figure 2. ChIP-seq results obtained with the Diagenode antibody directed against EHF
    ChIP was performed on sheared chromatin from 4,000,000 T47D cells using 2 µg of the Diagenode antibody against EHF (Cat. No. C15410363) as described above. The IP'd DNA was subsequently analysed on an Illumina HiSeq 4000. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 50 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the complete sequence and a 300 kb region of chromosome 1 (figure 2A and B) and in two genomic regions surrounding the ITGA2 and OAS3 positive control genes, respectively (figure 2C and D).

    ELISA

    Figure 3. Determination of the antibody titer
    To determine the titer of the antibody, an ELISA was performed using a serial dilution of Diagenode antibody directed against EHF (Cat. No. C15410363). The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:98,000.

  •  実験手法
  •  資料
    EHF polyclonal antibody DATASHEET
    Polyclonal antibody raised in rabbit against human EHF (ETS Homologous Factor), using two synthet...
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: EHF polyclonal antibody (Diagenode Cat# C15410363 Lot# A2883P). Click here to copy to clipboard.

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イベント

  • Molecular Biology Society of Japan
    Fukuoka
    Dec 3-Dec 6, 2019
  • ASH annual meeting
    Orlando, FL
    Dec 7-Dec 10, 2019
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