ChIPに適切なフラグメントサイズを得るためには、架橋クロマチンを断片化するための最適条件を確立することが重要です。 通常、このプロセスは超音波処理条件の最適化とSDS濃度の最適化の両方が必要になり、とても手間が掛かります。DiagenodeのChromatin Shearing Optimization Kitsなら、最適化が迅速かつ容易になるだけでなく、さまざまな濃度のSDSを含む最適化試薬をお試しいただけます。 さらに、このChromatin Shearing Optimization KitとChIP用のキットを一緒にクロマチン調製の最適化に使用できます。
|
SDS concentration |
< 0.1% |
0.2% |
0.5% |
1% |
|
Nuclei isolation |
Yes |
Yes |
Yes |
No |
|
Allows for shearing of... cells |
100 million cells |
100 million cells |
100 million cells |
100 million cells |
|
Corresponding to shearing buffers from |
iDeal ChIP-seq kit for Histones |
iDeal ChIP-seq Kit for Transcription Factors |
HighCell# ChIP kit |
True MicroChIP kit LowCell# ChIP kit |
| カタログ番号 | 製品 | フォーマット | 価格 | |
|---|---|---|---|---|
| C01020013 |
Chromatin EasyShear Kit - Low SDS Old name of the kit: Chromatin shearing optimization kit – Low SDS (iDeal Kit for TFs) A high quality chromatin preparation is very complex and requir... |
24 rxns | $305.00 | |
| C01020010 |
Chromatin EasyShear Kit – Ultra Low SDS Old name of the kit: Chromatin shearing optimization kit – Ultra Low SDS A high quality chromatin preparation is very complex and requires a lot of op... |
24 rxns | $305.00 | |
| C01020014 |
Chromatin Shearing Optimization Kit (Universal Plant ChIP-seq kit) The first critical step of a successful ChIP experiment is the best preparation of sheared chromatin. This Chromatin Shearing Optimization Kit is designed to... |
12 rxns | $305.00 | |
| C01020012 |
Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit) 洗浄剤は、クロマチン断片化の功のために不可欠です。 Diagenodeは、様々なワークフローに合わせた異なるSDS濃度の4つのキットを取り揃えています。 これらのキットは、iDeal ChIP-seq kit for Histones (< 0.1% SDS)、iDeal ChIP-seq kit for ... |
100 million cells | $305.00 |
| カタログ番号 | 製品 | フォーマット | 価格 | |
|---|---|---|---|---|
| C01020011 |
Chromatin Shearing Optimization Kit - Medium SDS (HighCell# ChIP kit) Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. The... |
100 million cells | $305.00 | |
| C01020022 |
Shearing Optimization kit The first critical step of a successful ChIP experiment is the preparation of sheared chromatin. Our Shearing Optimization Kit is perfect for obtai... |
40 rxns | $615.00 |
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