クロマチン機能

クロマチン断片化

ChIPに適切なフラグメントサイズを得るためには、架橋クロマチンを断片化するための最適条件を確立することが重要です。通常、このプロセスは超音波処理条件の最適化とSDS濃度の最適化の両方が必要になり、とても手間が掛かります。DiagenodeのChromatin　Shearing　Optimization Kitsなら、最適化が迅速かつ容易になるだけでなく、さまざまな濃度のSDSを含む最適化試薬をお試しいただけます。さらに、このChromatin Shearing Optimization KitとChIP用のキットを一緒にクロマチン調製の最適化に使用できます。

	Chromatin Shearing Kit Low SDS (for Histone)	Chromatin Shearing Kit Low SDS (for TF)	Chromatin Shearing Kit Medium SDS	Chromatin Shearing Kit High SDS
SDS concentration	< 0.1%	0.2%	0.5%	1%
Nuclei isolation	Yes	Yes	Yes	No
Allows for shearing of... cells	100 million cells	100 million cells	100 million cells	100 million cells
Corresponding to shearing buffers from	iDeal ChIP-seq kit for Histones	iDeal ChIP-seq Kit for Transcription Factors	HighCell# ChIP kit	True MicroChIP kit LowCell# ChIP kit

SDS-optimized chromatin shearing

カタログ番号	製品	フォーマット	価格
C01020013	Chromatin EasyShear Kit - Low SDS Old name of the kit: Chromatin shearing optimization kit – Low SDS (iDeal Kit for TFs) A high quality chromatin preparation is very complex and requir...	24 rxns	$305.00
C01020010	Chromatin EasyShear Kit – Ultra Low SDS Old name of the kit: Chromatin shearing optimization kit – Ultra Low SDS A high quality chromatin preparation is very complex and requires a lot of op...	24 rxns	$305.00
C01020014	Chromatin Shearing Optimization Kit (Universal Plant ChIP-seq kit) The first critical step of a successful ChIP experiment is the best preparation of sheared chromatin. This Chromatin Shearing Optimization Kit is designed to...	12 rxns	$305.00
C01020012	Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit) 洗浄剤は、クロマチン断片化の功のために不可欠です。 Diagenodeは、様々なワークフローに合わせた異なるSDS濃度の4つのキットを取り揃えています。これらのキットは、iDeal ChIP-seq kit for Histones (< 0.1% SDS)、iDeal ChIP-seq kit for ...	100 million cells	$305.00

その他の最適化専用キットとバッファー

カタログ番号	製品	フォーマット	価格
C01020011	Chromatin Shearing Optimization Kit - Medium SDS (HighCell# ChIP kit) Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. The...	100 million cells	$305.00
C01020022	Shearing Optimization kit The first critical step of a successful ChIP experiment is the preparation of sheared chromatin. Our Shearing Optimization Kit is perfect for obtai...	40 rxns	$615.00

CATS White paper

Thank you for your interest in our RNA-seq solutions.
Kindly leave your details below before proceeding to the white paper download.

Contact Information

First name

Email verification^*

Tweets by Diagenode

イベント

すべてのイベントを見る

ニュース

すべてのニュースを見る

The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.

ABOUT SSL CERTIFICATES

メールアドレス

Set country preference

To ensure you see the information most relevant to you, please select your country. Please note that your browser will need to be configured to accept cookies.

Diagenode will process your personal data in strict accordance with its privacy policy. This will include sending you updates about us, our products, and resources we think would be of interest to you.

>>> Click for Diagenode’s approach to COVID-19

クロマチン断片化

イベント

ニュース