Protocols

Protocols

Download Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor^® Standard, Plus or Pico
This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chromatin can be used for chromatin immunoprecipitation assays using Diagenode’s iDeal ChIP-seq ki...

Download ChIP - Just 4 steps to foolproofing fragmentation for ChIP using Bioruptor^® Standard, Plus or Pico
Just follow these four steps for shearing chromatin, and you’ll be well on your way to excellent ChIP results without the troubleshooting headache.

Download Chromatin shearing with the Bioruptor^® Pico 500 µl - 2 ml
Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. ...

Download NEBNext^® ChIP-Seq Library Prep for Illumina - IP-Star^® Compact -
The “NEBNext_ChIP-Seq_Illumina” protocol on the IP-Star® is using the standard “NEBNext® ChIP-Seq Library Prep for Illumina” kit and reagents from NEB®. This kit...

Download DNA Shearing for Bioruptor^® Pico (2013-2019) - Standard 100 μl Volume
Standard protocols for programmable DNA size distributions, excellent reproducibility, and high dsDNA yields with Bioruptor® Pico.

Download TruSeq DNA sample preparation - IP-Star^® Compact
The “TruSeq DNA SamplePrep” protocol on the IP-Star® is using the standard “TruSeqTM DNA Sample Preparation” kit and reagents from Illumina®. It provides flexibility...

Download ChIP from plant material with the LowCell# ChIP Kit
As preparation of plant material for Chromatin Immunoprecipiation differs essentially from preparation of culture cells, the LowCell# ChIP protocol has to be adapted respectively. We recommend ther...

Download FFPE DNA extraction protocol using the Bioruptor®
The number of archival formalin-fixed paraffin embedded (FFPE) samples is in the millions, providing an invaluable repository of information for genetic analysis. These samples can be analyzed for ...

Download Chromatin shearing with the Bioruptor Pico 10 µl - 300 µl
HeLa cells are fixed with 1% molecular grade formaldehyde (for 8 min at room temperature). Nucleus isolation is performed using buffers and reagents of Diagenode’s Chromatin Shearing Optimiza...

Download DNA Shearing for Bioruptor® XL
We highly recommend to use the tube holder for 0.5/0.65 ml tubes for the Bioruptor® XL (Cat. No. XL-pack 0.5) and the corresponding Bioruptor® 0.5 ml Microtubes for DNA Shearing (Cat. No. W...

Download ChIPettor Protocols
ChIPettor™ System for Histones and Transcription Factors. These protocols have been optimised for 1M cells per IP for Histones and 4 M cells per IP for Transcription Factors, although it is ...

Download Dot blot protocol for 5-hydroxymethylcytosine monoclonal rat antibody

Download DNA Shearing with Bioruptor® Pico (2013-2019) - Small Volume (10 µl)
For DNA shearing we highly recommend to use the 0.1 ml tube holder & tube adaptors (Cat. No. B01200041) and the corresponding 0.1 ml Bioruptor® Microtubes (Cat. No. C30010015).

Download RNA Shearing for Bioruptor® NGS and Pico
The Diagenode ACT (Adaptative Cavitation Transfer technology) process is highly reproducible, however attention must be paid to the following operating conditions to ensure best RNA shearing r...

Download ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus
A review of the critical points for chromatin shearing.

Download TruSeqTM ChIP Sample Preparation - IP-Star® Compact -
The “TruSeq ChIP SamplePrep” protocol on the IP-Star® is using the standard “TruSeqTM ChIP Sample Preparation” kit and reagents from Illumina®. It provides flexibili...

Download PCR amplicons shearing using the Bioruptor Pico
With the Bioruptor® Pico and the former model Bioruptor® NGS it is possible to shear PCR amplicons of at least 500 bp in size. While PCR fragments bigger than 700 bp are sheared accord...

Download Protein extraction from Tissues and Cultured Cells using Bioruptor® Standard & Plus
Protein extraction from tissues and cultured cells is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Effici...

Download Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®
The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using Bioruptor&...

Download DNA Shearing for Bioruptor® Pico (2013-2019) - 50 μl Volume
For DNA shearing in 50 μl we highly recommend to use the 0.1 ml tube holder & tube adaptors (Cat. No. B01200041) and the corresponding 0.1 ml Bioruptor® Microtubes (Cat. No. C30010015).

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