Diagenode

Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®

The regular protocol for the extraction of histone requires an acid extraction, making impossible the detection of any other cytoplasmic and nuclear proteins from the same extract. Using Bioruptor®, Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes developed a protocol allowing the simultaneous extraction of histone and other proteins.

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