|Activity Test - Procedure for immunoprecipitation of native chromatin proteins|
Proteins play role in almost all cellular processes, and the study of their activity is crucial f...
|Bacterial Cell Disruption using Bioruptor® Standard, Plus or XL|
For cell lysis, we highly recommend using 1.5 ml TPX microtubes (Cat. No. M-50050) or 10 ml tubes...
|ChIP - The Ultimate Guide for Chromatin Shearing Optimization with Bioruptor® Standard and Plus|
A review of the critical points for chromatin shearing.
|Chromatin shearing from tissue protocol using Diagenode’s Chromatin shearing optimization kit - Low SDS and Bioruptor® Standard, Plus or Pico|
This protocol describes the chromatin preparation from fresh or frozen tissues. The isolated chro...
|DNA Shearing for Bioruptor® Standard and Plus|
For DNA shearing we highly recommend to use the tube holder for 0.5/0.65 ml tubes (Cat. No. UCD-p...
|RNA extraction from tissue using Bioruptor® (Standard/Plus) and RNA extraction kit|
Isolation of intact RNA is essential for many techniques used in gene expression analysis. Effici...
|TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®|
The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein com...
|Western Blot - Procedure for simultaneous extraction of cytoplasmic, nuclear and chromatin protein with Bioruptor®|
The regular protocol for the extraction of histone requires an acid extraction, making impossible...