Chromatin shearing with the Bioruptor Pico 10 µl - 300 µl

HeLa cells are fixed with 1% molecular grade formaldehyde (for 8 min at room temperature). Nucleus isolation is performed using buffers and reagents of Diagenode’s Chromatin Shearing Optimization kit - Low SDS (Cat. No. AA-001-0100) 1x10e6 cells are then resuspended in 100 μl Shearing Buffer prior to chromatin shearing.

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