Diagenode

1.5 ml Picoruptor Microtubes with Caps

カタログ番号
フォーマット
価格
C30010016
300 tubes
$120.00

The 1.5 ml Picoruptor Microtubes with Caps for chromatin shearing strongly improve chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Picoruptor (B01060001) and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 100 μl - 300 μl.

  •  プロトコル集
    Chromatin shearing with the Bioruptor Pico 10 µl - 300 µl
    HeLa cells are fixed with 1% molecular grade formaldehyde (for 8 min at room temperature). Nucleu...
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    DNA Shearing for Bioruptor® Pico - Standard 100 μl Volume
    Standard protocols for programmable DNA size distributions, excellent reproducibility, and high d...
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    FFPE DNA extraction protocol using the Bioruptor®
    The number of archival formalin-fixed paraffin embedded (FFPE) samples is in the millions, provid...
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    Protein extraction from Tissues and Cultured Cells using Bioruptor® Pico
    Protein extraction from tissues and cultured cells is the first step for many biochemical and ana...
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  •  実験手法
    DNA/RNA断片
    近年の次世代シーケンシング(NGS)の進歩はゲノミクスや生物学に革命をもたらしました。 この成長により、最適なサンプル調製、ゲノムライブラリー構築には欠かせない高度技術の要求、必要性がさらに高まっています。 最適なライブラリー調製に最も重要な要因の一つは、シーケンシングされるDNAの品質です。 まずは、DNAを効果的かつ一貫してシーケンシングプラットフォームに応じた適切な断片サイズに切断し、具体的で信頼できるNGS結果を実現する必要があります。 Picoruptor and Megar... Read more
    クロマチン断片
    ChIPの成功に最も重要なステップは細胞の固定と溶解、そしてクロマチンの断片化の両方です。 研究者の中には、これらのステップの両方の重要な性質を見過すことがあります。断片化工程の不一致を解消するDiagenodeのPicoruptor®は、最先端の超音波ACT(Adaptive Cavitation Technology)を駆使し、効率的にクロマチンを断片します。ACTは、穏やかかつ非常に有効な断片力を用いて、ChIP実験に必須の高いIP効率および感度を持つ最高のクロマチン品質... Read more
    ホルマリン固定 DNA抽出
    Diagenode's high yields FFPE DNA extraction using Bioruptor® is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraf... Read more
  •  資料
    1.5 ml Bioruptor® Microtubes DATASHEET
    The 1.5 ml Bioruptor® Microtubes C30010016 for chromatin shearing strongly impro...
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    Which tubes for which Bioruptor®? QUICK GUIDE
    Diagenode tubes were developed specifically for use with the Bioruptor®. They ensure a maximu...
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  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: 1.5 ml Picoruptor Microtubes with Caps (Diagenode Cat# C30010016). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    The role of TCF3 as potential master regulator in blastemal Wilms tumors.
    Kehl T, Schneider L, Kattler K, Stöckel D, Wegert J, Gerstner N, Ludwig N, Distler U, Tenzer S, Gessler M, Walter J, Keller A, Graf N, Meese E, Lenhof HP
    Wilms tumors are the most common type of pediatric kidney tumors. While the overall prognosis for patients is favorable, especially tumors that exhibit a blastemal subtype after preoperative chemotherapy have a poor prognosis. For an improved risk assessment and therapy stratification, it is essential to identify th...

    Transcriptome-wide dynamics of extensive m6A mRNA methylation during Plasmodium falciparum blood-stage development
    Sebastian Baumgarten, Jessica M. Bryant, Ameya Sinha, Thibaud Reyser, Peter R. Preiser, Peter C. Dedon, Artur Scherf
    Malaria pathogenesis results from the asexual replication of Plasmodium falciparum within human red blood cells, which relies on a precisely timed cascade of gene expression over a 48-hour life cycle. Although substantial post-transcriptional regulation of this hardwired program has been observed, it remains unclear...

    Dot1 promotes H2B ubiquitination by a methyltransferase-independent mechanism.
    van Welsem T, Korthout T, Ekkebus R, Morais D, Molenaar TM, van Harten K, Poramba-Liyanage DW, Sun SM, Lenstra TL, Srivas R, Ideker T, Holstege FCP, van Attikum H, El Oualid F, Ovaa H, Stulemeijer IJE, Vlaming H, van Leeuwen F
    The histone methyltransferase Dot1 is conserved from yeast to human and methylates lysine 79 of histone H3 (H3K79) on the core of the nucleosome. H3K79 methylation by Dot1 affects gene expression and the response to DNA damage, and is enhanced by monoubiquitination of the C-terminus of histone H2B (H2Bub1). To gain ...

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