You are about to request a quote for Picoruptor 2 sonication device . Fill out the form below and we will be in touch with you very soon.
All * fields are mandatory
環境研究、毒物学、ゲノミクスおよびエピゲノミクス、癌研究、幹細胞開発、神経科学、臨床応用、農業、その他多数を含むさまざまな研究分野でのサンプル調製において、最高のプロセスを目指して設計開発されたPicoruptorと共にDiagenodeは、2004年以来断片化に関する専門知識と経験を積み重ねてきました。
Picoruptorは、断片化おける最新のイノベーションであり、150 bpから1 kbのサンプルを断片化できるオールインワンのシアリングシステムとして最も注目されています。柔軟なサンプル量(20 µl-2 ml)とサンプルの高速並行処理(最大16サンプルまで)に欠かせないシアリングアクセサリーと消耗品もラインナップ。 冷却システムにより、高精度の温度制御が保証されます。 ユーザーフレンドリーなインターフェースはシンプルでありながら高度なモードを提供し、研究者のニーズに合わせて設計されています。ビギナーユーザーとエキスパートユーザーの両方に適切なレベル設定を提供します。
Diagenodeの断片化キット、完全検証済みチューブは最高品質だけでなく、高いコストパフォーマンスをお届けします。
アプリケーションの多様性::
Name | Catalog number | Throughput |
Tube holder for 0.2 ml tubes | B01201144 | 16 samples |
Tube holder for 0.65 ml tubes | B01201143 | 12 samples |
Tube holder for 1.5 ml tubes | B01201140 | 6 samples |
15 ml sonication accessories | B01200016 | 6 samples |
Name | Catalog Number |
0.2 ml Pico Microtubes | C30010020 |
0.65 ml Pico Microtubes | C30010011 |
1.5 ml Pico Microtubes | C30010016 |
15 ml Pico Tubes | C30010017 |
15 ml Pico Tubes & sonication beads | C01020031 |
DNA shearing guide DNA shearing for Next-Generation Sequencing with the Bioruptor Pico | Download |
Diagenode has optimized a range of solutions for successful chromatin preparation. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.
For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.
SAMPLE TYPE | SAMPLE INPUT | KIT | SDS CONCENTRATION |
NUCLEI ISOLATION |
||
---|---|---|---|---|---|---|
CELLS
|
< 100,000 | Chromatin EasyShear Kit High SDS |
1% | |||
CELLS
|
> 100,000 | Chromatin EasyShear Kit Ultra Low SDS |
< 0.1% | |||
TISSUE
|
Chromatin EasyShear Kit Ultra Low SDS |
< 0.1% | ||||
PLANT TISSUE
|
Chromatin EasyShear Kit for Plant |
0.5% | ||||
FFPE SAMPLES
|
Chromatin EasyShear Kit Low SDS |
0.2% | ||||
CELLS
|
Chromatin EasyShear Kit Low SDS |
0.2% | ||||
TISSUE
|
||||||
FFPE SAMPLES
|
||||||
Chromatin Shearing Guide PROTOCOL Guide for successful chromatin preparation using the Bioruptor Pico | Download |
DNA shearing guide PROTOCOL DNA shearing for Next-Generation Sequencing with the Bioruptor Pico | Download |
Which tubes for Bioruptor® Pico DOCUMENT | Download |
Unlock Low-Input 3D Genome Analysis with the Arima-HiC Kit APPLICATION NOTE By combining the optimized chemistry of the Arima-HiC kit along with new low input protocols, the... | Download |
Datasheet of Bioruptor tubes DATASHEET Datasheet of Diagenode tubes for Bioruptor Pico and Bioruptor Plus. | Download |
Critical steps for Bioruptor® maintenance and efficient shearing DOCUMENT | Download |
How to properly cite this product in your workDiagenode strongly recommends using this: Picoruptor 2 sonication device (Diagenode Cat# B01080010). Click here to copy to clipboard. Using our products in your publication? Let us know! |
LEO1 Is Required for Efficient Entry into Quiescence, Control of H3K9 Methylation and Gene Expression in Human Fibroblasts |
DeSUMOylation of chromatin-bound proteins limits the rapidtranscriptional reprogramming induced by daunorubicin in acute myeloidleukemias. |
RNA polymerase II CTD is dispensable for transcription and requiredfor termination in human cells. |
Targeting lymphoid-derived IL-17 signaling to delay skin aging. |
Nicotinamide N-methyltransferase sustains a core epigenetic programthat promotes metastatic colonization in breast cancer. |
SOX expression in prostate cancer drives resistance to nuclear hormonereceptor signaling inhibition through the WEE1/CDK1 signaling axis. |
The Effect of Metformin and Carbohydrate-Controlled Diet onDNA Methylation and Gene Expression in the Endometrium of Womenwith Polycystic Ovary Syndrome. |
Chromatin profiling identifies transcriptional readthrough as a conservedmechanism for piRNA biogenesis in mosquitoes. |
Activation of AKT induces EZH2-mediated β-catenin trimethylation incolorectal cancer. |
Detailed molecular and epigenetic characterization of the Pig IPECJ2and Chicken SL-29 cell lines |
Signal-induced enhancer activation requires Ku70 to readtopoisomerase1-DNA covalent complexes. |
Cotranscriptional demethylation induces global loss of H3K4me2 fromactive genes in Arabidopsis |
Epigenetic regulation of plastin 3 expression by the macrosatelliteDXZ4 and the transcriptional regulator CHD4. |
A dataset of definitive endoderm and hepatocyte differentiations fromhuman induced pluripotent stem cells. |
The mineralocorticoid receptor modulates timing and location of genomicbinding by glucocorticoid receptor in response to synthetic glucocorticoidsin keratinocytes. |
Gene Regulatory Interactions at Lamina-Associated Domains |
The aryl hydrocarbon receptor cell intrinsically promotes resident memoryCD8 T cell differentiation and function. |
Impact of Fetal Exposure to Endocrine Disrupting ChemicalMixtures on FOXA3 Gene and Protein Expression in Adult RatTestes. |
Transfer of blocker-based qPCR reactions for DNA methylation analysisinto a microfluidic LoC system using thermal modeling. |
Intranasal administration of Acinetobacter lwoffii in a murine model ofasthma induces IL-6-mediated protection associated with cecal microbiotachanges. |
Trichoderma root colonization triggers epigenetic changes in jasmonic andsalicylic acid pathway-related genes. |
DNA sequence and chromatin modifiers cooperate to confer epigeneticbistability at imprinting control regions. |
Smc5/6 silences episomal transcription by a three-step function. |
Exploration of nuclear body-enhanced sumoylation reveals that PMLrepresses 2-cell features of embryonic stem cells. |
Loss of epigenetic regulation disrupts lineage integrity, inducesaberrant alveogenesis and promotes breast cancer. |
RAD51 protects human cells from transcription-replication conflicts. |
The Arabidopsis APOLO and human UPAT sequence-unrelated longnoncoding RNAs can modulate DNA and histone methylation machineries inplants. |
Prolonged FOS activity disrupts a global myogenic transcriptionalprogram by altering 3D chromatin architecture in primary muscleprogenitor cells. |
Androgen-Induced MIG6 Regulates Phosphorylation ofRetinoblastoma Protein and AKT to Counteract Non-Genomic ARSignaling in Prostate Cancer Cells. |
Variation in PU.1 binding and chromatin looping at neutrophil enhancersinfluences autoimmune disease susceptibility |
CREBBP/EP300 acetyltransferase inhibition disrupts FOXA1-bound enhancers to inhibit the proliferation of ER+ breast cancer cells. |
Transient regulation of focal adhesion via Tensin3 is required fornascent oligodendrocyte differentiation |
The long noncoding RNA H19 regulates tumor plasticity inneuroendocrine prostate cancer |
Epromoters function as a hub to recruit key transcription factorsrequired for the inflammatory response |
Differential contribution to gene expression prediction of histonemodifications at enhancers or promoters. |
Atg7 deficiency in microglia drives an altered transcriptomic profileassociated with an impaired neuroinflammatory response |
Lasp1 regulates adherens junction dynamics and fibroblast transformationin destructive arthritis |
The lncRNA and the transcription factor WRKY42 target common cell wallEXTENSIN encoding genes to trigger root hair cell elongation. |
Placental uptake and metabolism of 25(OH)Vitamin D determines itsactivity within the fetoplacental unit |
Waves of sumoylation support transcription dynamics during adipocytedifferentiation |
Androgen receptor positively regulates gonadotropin-releasing hormonereceptor in pituitary gonadotropes. |
Genetic perturbation of PU.1 binding and chromatin looping at neutrophilenhancers associates with autoimmune disease. |
Fra-1 regulates its target genes via binding to remote enhancers withoutexerting major control on chromatin architecture in triple negative breastcancers. |
Cell-specific alterations inPitx1regulatory landscape activation caused bythe loss of a single enhancer |
Transgenic mice for in vivo epigenome editing with CRISPR-based systems |
Transcriptional programming drives Ibrutinib-resistance evolution in mantlecell lymphoma. |
Coordinated changes in gene expression, H1 variant distribution and genome3D conformation in response to H1 depletion |
REPROGRAMMING CBX8-PRC1 FUNCTION WITH A POSITIVE ALLOSTERICMODULATOR |
Germline activity of the heat shock factor HSF-1 programs theinsulin-receptor daf-2 in C. elegans |
The epigenetic landscape in purified myonuclei from fast and slow muscles |
The glucocorticoid receptor recruits the COMPASS complex to regulateinflammatory transcription at macrophage enhancers. |
A distinct metabolic response characterizes sensitivity to EZH2inhibition in multiple myeloma. |
BAF complexes drive proliferation and block myogenic differentiation in fusion-positive rhabdomyosarcoma |
A Tumor Suppressor Enhancer of PTEN in T-cell development and leukemia |
Stronger induction of trained immunity by mucosal BCG or MTBVAC vaccination compared to standard intradermal vaccination. |
Postoperative abdominal sepsis induces selective and persistent changes inCTCF binding within the MHC-II region of human monocytes. |
S-adenosyl-l-homocysteine hydrolase links methionine metabolism to thecircadian clock and chromatin remodeling. |
Genomic profiling of T-cell activation suggests increased sensitivity ofmemory T cells to CD28 costimulation. |
A genetic variant controls interferon-β gene expression in human myeloidcells by preventing C/EBP-β binding on a conserved enhancer. |
BCG Vaccination Induces Long-Term Functional Reprogramming of HumanNeutrophils. |
Macrophage Immune Memory Controls Endometriosis in Mice and Humans. |
UTX/KDM6A suppresses AP-1 and a gliogenesis program during neuraldifferentiation of human pluripotent stem cells. |
Epigenetic regulation of the lineage specificity of primary human dermallymphatic and blood vascular endothelial cells. |
Combined treatment with CBP and BET inhibitors reverses inadvertentactivation of detrimental super enhancer programs in DIPG cells. |
Methylation in pericytes after acute injury promotes chronic kidneydisease. |
Exploring the virulence gene interactome with CRISPR/dCas9 in the humanmalaria parasite. |
Targeted bisulfite sequencing for biomarker discovery. |
Battle of the sex chromosomes: competition between X- and Y-chromosomeencoded proteins for partner interaction and chromatin occupancy drivesmulti-copy gene expression and evolution in muroid rodents. |
BET protein inhibition sensitizes glioblastoma cells to temozolomidetreatment by attenuating MGMT expression |
You are about to request a quote for Tube holder for 0.65 ml tubes - Picoruptor. Fill out the form below and we will be in touch with you very soon.
All * fields are mandatory
Notice (8): Undefined variable: solution_of_interest [APP/View/Products/view.ctp, line 755]Code Context<!-- BEGIN: REQUEST_FORM MODAL -->
<div id="request_formModal" class="reveal-modal medium" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<?= $this->element('Forms/simple_form', array('solution_of_interest' => $solution_of_interest, 'header' => $header, 'message' => $message, 'campaign_id' => $campaign_id)) ?>
$viewFile = '/home/website-server/www/app/View/Products/view.ctp' $dataForView = array( 'language' => 'jp', 'meta_keywords' => '', 'meta_description' => '', 'meta_title' => '', 'product' => array( 'Product' => array( 'id' => '3046', 'antibody_id' => null, 'name' => 'Picoruptor 2 sonication device ', 'description' => '<p></p> <p>環境研究、毒物学、ゲノミクスおよびエピゲノミクス、癌研究、幹細胞開発、神経科学、臨床応用、農業、その他多数を含むさまざまな研究分野でのサンプル調製において、最高のプロセスを目指して設計開発されたPicoruptorと共にDiagenodeは、2004年以来断片化に関する専門知識と経験を積み重ねてきました。</p> <p>Picoruptorは、断片化おける最新のイノベーションであり、150 bpから1 kbのサンプルを断片化できるオールインワンのシアリングシステムとして最も注目されています。柔軟なサンプル量(20 µl-2 ml)とサンプルの高速並行処理(最大16サンプルまで)に欠かせないシアリングアクセサリーと消耗品もラインナップ。 冷却システムにより、高精度の温度制御が保証されます。 ユーザーフレンドリーなインターフェースはシンプルでありながら高度なモードを提供し、研究者のニーズに合わせて設計されています。ビギナーユーザーとエキスパートユーザーの両方に適切なレベル設定を提供します。</p> <p>Diagenodeの断片化キット、完全検証済みチューブは最高品質だけでなく、高いコストパフォーマンスをお届けします。</p> <p><strong>アプリケーションの多様性:</strong>:</p> <ul> <li>次世代シーケンスのためのDNA断片化</li> <li>クロマチン断片化</li> <li>RNA断片化</li> <li>組織および細胞からのタンパク質抽出(質量分析用)</li> <li>FFPE DNA抽出</li> <li>タンパク質凝集研究</li> </ul> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="gtx-trans" style="position: absolute; left: -171px; top: 17.6px;"> <div class="gtx-trans-icon"></div> </div>', 'label1' => '仕様書', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'Picoruptorのアクセサリーと消耗品はこちら', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => '利用可能な断片化キット', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'bioruptorpico2', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:28:50', 'created' => '2020-01-09 16:27:17', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Application' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ) ), 'Category' => array( (int) 0 => array( [maximum depth reached] ) ), 'Document' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ) ), 'Feature' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ) ), 'Image' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( [maximum depth reached] ) ), 'Publication' => array( (int) 0 => array( [maximum depth reached] ), (int) 1 => array( [maximum depth reached] ), (int) 2 => array( [maximum depth reached] ), (int) 3 => array( [maximum depth reached] ), (int) 4 => array( [maximum depth reached] ), (int) 5 => array( [maximum depth reached] ), (int) 6 => array( [maximum depth reached] ), (int) 7 => array( [maximum depth reached] ), (int) 8 => array( [maximum depth reached] ), (int) 9 => array( [maximum depth reached] ), (int) 10 => array( [maximum depth reached] ), (int) 11 => array( [maximum depth reached] ), (int) 12 => array( [maximum depth reached] ), (int) 13 => array( [maximum depth reached] ), (int) 14 => array( [maximum depth reached] ), (int) 15 => array( [maximum depth reached] ), (int) 16 => array( [maximum depth reached] ), (int) 17 => array( [maximum depth reached] ), (int) 18 => array( [maximum depth reached] ), (int) 19 => array( [maximum depth reached] ), (int) 20 => array( [maximum depth reached] ), (int) 21 => array( [maximum depth reached] ), (int) 22 => array( [maximum depth reached] ), (int) 23 => array( [maximum depth reached] ), (int) 24 => array( [maximum depth reached] ), (int) 25 => array( [maximum depth reached] ), (int) 26 => array( [maximum depth reached] ), (int) 27 => array( [maximum depth reached] ), (int) 28 => array( [maximum depth reached] ), (int) 29 => array( [maximum depth reached] ), (int) 30 => array( [maximum depth reached] ), (int) 31 => array( [maximum depth reached] ), (int) 32 => array( [maximum depth reached] ), (int) 33 => array( [maximum depth reached] ), (int) 34 => array( [maximum depth reached] ), (int) 35 => array( [maximum depth reached] ), (int) 36 => array( [maximum depth reached] ), (int) 37 => array( [maximum depth reached] ), (int) 38 => array( [maximum depth reached] ), (int) 39 => array( [maximum depth reached] ), (int) 40 => array( [maximum depth reached] ), (int) 41 => array( [maximum depth reached] ), (int) 42 => array( [maximum depth reached] ), (int) 43 => array( [maximum depth reached] ), (int) 44 => array( [maximum depth reached] ), (int) 45 => array( [maximum depth reached] ), (int) 46 => array( [maximum depth reached] ), (int) 47 => array( [maximum depth reached] ), (int) 48 => array( [maximum depth reached] ), (int) 49 => array( [maximum depth reached] ), (int) 50 => array( [maximum depth reached] ), (int) 51 => array( [maximum depth reached] ), (int) 52 => array( [maximum depth reached] ), (int) 53 => array( [maximum depth reached] ), (int) 54 => array( [maximum depth reached] ), (int) 55 => array( [maximum depth reached] ), (int) 56 => array( [maximum depth reached] ), (int) 57 => array( [maximum depth reached] ), (int) 58 => array( [maximum depth reached] ), (int) 59 => array( [maximum depth reached] ), (int) 60 => array( [maximum depth reached] ), (int) 61 => array( [maximum depth reached] ), (int) 62 => array( [maximum depth reached] ), (int) 63 => array( [maximum depth reached] ), (int) 64 => array( [maximum depth reached] ), (int) 65 => array( [maximum depth reached] ), (int) 66 => array( [maximum depth reached] ), (int) 67 => array( [maximum depth reached] ), (int) 68 => array( [maximum depth reached] ) ), 'Testimonial' => array(), 'Area' => array(), 'SafetySheet' => array() ) ) $language = 'jp' $meta_keywords = '' $meta_description = '' $meta_title = '' $product = array( 'Product' => array( 'id' => '3046', 'antibody_id' => null, 'name' => 'Picoruptor 2 sonication device ', 'description' => '<p></p> <p>環境研究、毒物学、ゲノミクスおよびエピゲノミクス、癌研究、幹細胞開発、神経科学、臨床応用、農業、その他多数を含むさまざまな研究分野でのサンプル調製において、最高のプロセスを目指して設計開発されたPicoruptorと共にDiagenodeは、2004年以来断片化に関する専門知識と経験を積み重ねてきました。</p> <p>Picoruptorは、断片化おける最新のイノベーションであり、150 bpから1 kbのサンプルを断片化できるオールインワンのシアリングシステムとして最も注目されています。柔軟なサンプル量(20 µl-2 ml)とサンプルの高速並行処理(最大16サンプルまで)に欠かせないシアリングアクセサリーと消耗品もラインナップ。 冷却システムにより、高精度の温度制御が保証されます。 ユーザーフレンドリーなインターフェースはシンプルでありながら高度なモードを提供し、研究者のニーズに合わせて設計されています。ビギナーユーザーとエキスパートユーザーの両方に適切なレベル設定を提供します。</p> <p>Diagenodeの断片化キット、完全検証済みチューブは最高品質だけでなく、高いコストパフォーマンスをお届けします。</p> <p><strong>アプリケーションの多様性:</strong>:</p> <ul> <li>次世代シーケンスのためのDNA断片化</li> <li>クロマチン断片化</li> <li>RNA断片化</li> <li>組織および細胞からのタンパク質抽出(質量分析用)</li> <li>FFPE DNA抽出</li> <li>タンパク質凝集研究</li> </ul> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="gtx-trans" style="position: absolute; left: -171px; top: 17.6px;"> <div class="gtx-trans-icon"></div> </div>', 'label1' => '仕様書', 'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'label2' => 'Picoruptorのアクセサリーと消耗品はこちら', 'info2' => '<h3>Shearing Accessories</h3> <table style="width: 641px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 300px; height: 37px;"><strong>Name</strong></td> <td style="width: 171px; text-align: center; height: 37px;">Catalog number</td> <td style="width: 160px; text-align: center; height: 37px;">Throughput</td> </tr> </thead> <tbody> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td> </tr> <tr style="height: 38px;"> <td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td> <td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td> <td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> <tr style="height: 37px;"> <td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td> <td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td> <td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td> </tr> </tbody> </table> <h3>Shearing Consumables</h3> <table style="width: 646px;"> <thead> <tr style="background-color: #dddddd; height: 37px;"> <td style="width: 286px; height: 37px;"><strong>Name</strong></td> <td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td> </tr> </thead> <tbody> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td> </tr> <tr style="height: 37px;"> <td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td> <td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td> </tr> </tbody> </table> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p> <p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>', 'label3' => '利用可能な断片化キット', 'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p> <p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p> <h2>Kit choice guide</h2> <table style="border: 0;" valign="center"> <tbody> <tr style="background: #fff;"> <th class="text-center"></th> <th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th> <th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th> <th class="text-center" style="font-size: 17px;">KIT</th> <th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th> <th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td> <td class="text-center" style="border-bottom: 1px solid #dedede;"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td> <td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td> <td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center" style="font-size: 17px;">> 100,000</td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td> <td class="text-center" style="font-size: 17px;">< 0.1%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff; border-bottom: 1px solid #dedede;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">PLANT TISSUE</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td> <td class="text-center" style="font-size: 17px;">0.5%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> <td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td class="text-center" style="font-size: 17px;">0.2%</td> <td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td colspan="7"></td> </tr> <tr style="background: #fff;"> <td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">CELLS</div> </td> <td class="text-center"></td> <td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td> <td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td> <td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">TISSUE</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td class="text-center"> <div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div> </td> <td class="text-center"></td> </tr> <tr style="background: #fff;"> <td colspan="6"></td> </tr> </tbody> </table> <div class="extra-spaced"> <h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3> </div> <p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> </p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>', 'format' => '1 unit', 'catalog_number' => 'B01080010', 'old_catalog_number' => '', 'sf_code' => 'B01080010-', 'type' => 'ACC', 'search_order' => '00-Machine', 'price_EUR' => '27000', 'price_USD' => '30000', 'price_GBP' => '23500', 'price_JPY' => '4229550', 'price_CNY' => '/', 'price_AUD' => '75000', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => true, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => 'bioruptorpico2', 'meta_title' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2024-06-14 15:28:50', 'created' => '2020-01-09 16:27:17', 'locale' => 'jpn' ), 'Antibody' => array( 'host' => '*****', 'id' => null, 'name' => null, 'description' => null, 'clonality' => null, 'isotype' => null, 'lot' => null, 'concentration' => null, 'reactivity' => null, 'type' => null, 'purity' => null, 'classification' => null, 'application_table' => null, 'storage_conditions' => null, 'storage_buffer' => null, 'precautions' => null, 'uniprot_acc' => null, 'slug' => null, 'meta_keywords' => null, 'meta_description' => null, 'modified' => null, 'created' => null, 'select_label' => null ), 'Slave' => array(), 'Group' => array(), 'Related' => array( (int) 0 => array( 'id' => '3047', 'antibody_id' => null, 'name' => 'Tube holder for 1.5 ml tubes - Bioruptor<sup>®</sup> Pico', 'description' => '<p><span>The tube holder for 1.5 ml tubes has been especially designed for being used with the <strong>Bioruptor® Pico</strong> and to guarantee homogeneity of chromatin or DNA shearing. It allows for reproducible sonication of up to<strong> 6 x 1.5 ml tubes</strong> at a time with a <strong>sample volume range of 100 to 300 µl</strong>. The holder is made up of a tube holder and its specific dock that makes the sample preparation more ergonomic. <br /></span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B01201140', 'old_catalog_number' => '', 'sf_code' => 'B01201140-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '1650', 'price_USD' => '1850', 'price_GBP' => '1500', 'price_JPY' => '258470', 'price_CNY' => '', 'price_AUD' => '4625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => '1-5-ml-tube-holder-dock-for-bioruptor-pico', 'meta_title' => 'Tube holder for 1.5 ml tubes - Bioruptor® Pico', 'meta_keywords' => '', 'meta_description' => 'Tube holder for 1.5 ml tubes - Bioruptor® Pico', 'modified' => '2021-03-04 09:56:54', 'created' => '2020-01-21 10:28:11', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3048', 'antibody_id' => null, 'name' => 'Tube holder for 0.65 ml tubes - Picoruptor', 'description' => '<p><span>The tube holder for 0.65 ml tubes has been especially designed for being used with the <strong>Picoruptor</strong> and to guarantee homogeneity of chromatin and DNA shearing. It allows for reproducible sonication of up to<strong> 12 x 0.65 ml tubes</strong> at a time with a <strong>sample volume of 100 µl</strong>. The holder is made up of a tube holder and its specific dock that makes the sample preparation more ergonomic. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B01201143', 'old_catalog_number' => '', 'sf_code' => 'B01201143-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '1650', 'price_USD' => '1850', 'price_GBP' => '1500', 'price_JPY' => '258470', 'price_CNY' => '', 'price_AUD' => '4625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => true, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => '0-65-ml-tube-holder-dock-for-bioruptor-pico', 'meta_title' => 'Tube holder for 0.65 ml tubes - Bioruptor® Pico', 'meta_keywords' => '', 'meta_description' => 'Tube holder for 0.65 ml tubes - Bioruptor® Pico', 'modified' => '2020-01-21 17:57:46', 'created' => '2020-01-21 10:48:09', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '3049', 'antibody_id' => null, 'name' => 'Tube holder for 0.2 ml tubes - Bioruptor<sup>®</sup> Pico', 'description' => '<p><span>The tube holder for 0.2 ml tubes has been especially designed for being used with the <strong>Bioruptor® Pico</strong> and to guarantee homogeneity of chromatin and DNA shearing. It allows for reproducible sonication of up to<strong> 16 x 0.2 ml tubes</strong> at a time with a <strong>sample volume range of 20 to 100 µl</strong>. The holder is made up of a tube holder and its specific dock that makes the sample preparation more ergonomic. </span></p>', 'label1' => '', 'info1' => '', 'label2' => '', 'info2' => '', 'label3' => '', 'info3' => '', 'format' => '1 unit', 'catalog_number' => 'B01201144', 'old_catalog_number' => '', 'sf_code' => 'B01201144-', 'type' => 'ACC', 'search_order' => '', 'price_EUR' => '1650', 'price_USD' => '1850', 'price_GBP' => '1500', 'price_JPY' => '258470', 'price_CNY' => '', 'price_AUD' => '4625', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '', 'slug' => '0-2-ml-tube-holder-dock-for-bioruptor-pico', 'meta_title' => 'Tube holder for 0.2 ml tubes - Bioruptor® Pico', 'meta_keywords' => '', 'meta_description' => 'Tube holder for 0.2 ml tubes - Bioruptor® Pico', 'modified' => '2022-06-09 17:13:58', 'created' => '2020-01-21 11:00:25', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '1796', 'antibody_id' => null, 'name' => '15 ml sonication accessories for Bioruptor<sup>®</sup> Standard & Plus & Pico', 'description' => '<p><span>This tube holder has been designed for being used in combination with the Bioruptor® Standard, Plus and Pico for tissue disruption and cell lysis. </span></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label1' => '', 'info1' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label2' => '', 'info2' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'label3' => '', 'info3' => '<p></p> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script> <script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>', 'format' => '1 pack', 'catalog_number' => 'B01200016', 'old_catalog_number' => 'O-ring-15', 'sf_code' => 'B01200016-', 'type' => 'ACC', 'search_order' => '01-Accessory', 'price_EUR' => '300', 'price_USD' => '350', 'price_GBP' => '250', 'price_JPY' => '46995', 'price_CNY' => '', 'price_AUD' => '875', 'country' => 'ALL', 'except_countries' => 'None', 'quote' => false, 'in_stock' => false, 'featured' => false, 'no_promo' => false, 'online' => true, 'master' => true, 'last_datasheet_update' => '0000-00-00', 'slug' => '15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack', 'meta_title' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico', 'meta_keywords' => '', 'meta_description' => '15 ml sonication accessories for Bioruptor® Standard & Plus & Pico', 'modified' => '2022-01-25 04:00:28', 'created' => '2015-06-29 14:08:20', 'ProductsRelated' => array( [maximum depth reached] ), 'Image' => array( [maximum depth reached] ) ) ), 'Application' => array( (int) 0 => array( 'id' => '8', 'position' => '10', 'parent_id' => null, 'name' => 'Chromatin shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div> <div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div> <div class="small-12 medium-12 large-12 columns"><br /><br /></div> <div class="small-12 medium-12 large-12 columns"> <p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p> </div> <div class="small-12 medium-12 large-12 columns"> <div class="page" title="Page 7"> <table> <tbody> <tr valign="middle"> <td></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td> <td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>SDS concentration</strong></p> </td> <td style="text-align: center;"> <p>< 0.1%</p> </td> <td style="text-align: center;"> <p>0.2%</p> </td> <td style="text-align: center;"> <p>1%</p> </td> <td style="text-align: center;"> <p>0.5%</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Nuclei isolation</strong></p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> <td style="text-align: center;"> <p>No</p> </td> <td style="text-align: center;"> <p>Yes</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Allows for shearing of... cells/tissue</strong></p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>100 million cells</p> </td> <td style="text-align: center;"> <p>up to 25 g of tissue</p> </td> </tr> <tr valign="middle" style="background-color: #fff;"> <td> <p><strong>Corresponding to shearing buffers from</strong></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p> <p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p> </td> <td style="text-align: center;"> <p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p> </td> </tr> </tbody> </table> <p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'chromatin-shearing', 'meta_keywords' => 'Chromatin shearing,Chromatin Immunoprecipitation,Bioruptor,Sonication,Sonicator', 'meta_description' => 'Diagenode's Bioruptor® is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.', 'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode', 'modified' => '2017-11-15 10:14:02', 'created' => '2015-03-05 15:56:30', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '3', 'position' => '10', 'parent_id' => null, 'name' => '次世代シーケンシング', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns"> <h2 style="font-size: 22px;">DNA断片化、ライブラリー調製、自動化:NGSのワンストップショップ</h2> <table class="small-12 medium-12 large-12 columns"> <tbody> <tr> <th class="small-12 medium-12 large-12 columns"> <h4>1. 断片化装置を選択してください:150 bp〜75 kbの範囲でDNAを断片化します。</h4> </th> </tr> <tr style="background-color: #ffffff;"> <td class="small-12 medium-12 large-12 columns"></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-pico-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/bioruptor_pico.jpg" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/megaruptor2-1-unit"><img src="https://www.diagenode.com/img/product/shearing_technologies/B06010001_megaruptor2.jpg" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/bioruptor-one-sonication-device"><img src="https://www.diagenode.com/img/product/shearing_technologies/br-one-profil.png" /></a></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns">5μlまで断片化:150 bp〜2 kb<br />NGS DNAライブラリー調製およびFFPE核酸抽出に最適で、</td> <td class="small-4 medium-4 large-4 columns">2 kb〜75 kbの範囲をできます。<br />メイトペアライブラリー調製および長いフラグメントDNAシーケンシングに最適で、この軽量デスクトップデバイスで</td> <td class="small-4 medium-4 large-4 columns">20または50μlの断片化が可能です。</td> </tr> </tbody> </table> <table class="small-12 medium-12 large-12 columns"> <tbody> <tr> <th class="small-8 medium-8 large-8 columns"> <h4>2. 最適化されたライブラリー調整キットを選択してください。</h4> </th> <th class="small-4 medium-4 large-4 columns"> <h4>3. ライブラリー前処理自動化を選択して、比類のないデータ再現性を実感</h4> </th> </tr> <tr style="background-color: #ffffff;"> <td class="small-12 medium-12 large-12 columns"></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns"><a href="../p/microplex-library-preparation-kit-v2-x12-12-indices-12-rxns"><img src="https://www.diagenode.com/img/product/kits/microPlex_library_preparation.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/ideal-library-preparation-kit-x24-incl-index-primer-set-1-24-rxns"><img src="https://www.diagenode.com/img/product/kits/box_kit.jpg" style="display: block; margin-left: auto; margin-right: auto;" height="173" width="250" /></a></td> <td class="small-4 medium-4 large-4 columns"><a href="../p/sx-8g-ip-star-compact-automated-system-1-unit"><img src="https://www.diagenode.com/img/product/automation/B03000002%20_ipstar_compact.png" style="display: block; margin-left: auto; margin-right: auto;" /></a></td> </tr> <tr> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">50pgの低入力:MicroPlex Library Preparation Kit</td> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">5ng以上:iDeal Library Preparation Kit</td> <td class="small-4 medium-4 large-4 columns" style="text-align: center;">Achieve great NGS data easily</td> </tr> </tbody> </table> </div> </div> <blockquote> <div class="row"> <div class="small-12 medium-12 large-12 columns"><span class="label" style="margin-bottom: 16px; margin-left: -22px; font-size: 15px;">DiagenodeがNGS研究にぴったりなプロバイダーである理由</span> <p>Diagenodeは15年以上もエピジェネティクス研究に専念、専門としています。 ChIP研究クロマチン用のユニークな断片化システムの開発から始まり、 専門知識を活かし、5μlのせん断体積まで可能で、NGS DNAライブラリーの調製に最適な最先端DNA断片化装置の開発にたどり着きました。 我々は以来、ChIP-seq、Methyl-seq、NGSライブラリー調製用キットを研究開発し、業界をリードする免疫沈降研究と同様に、ライブラリー調製を自動化および完結させる独自の自動化システムを開発にも成功しました。</p> <ul> <li>信頼されるせん断装置</li> <li>様々なインプットからのライブラリ作成キット</li> <li>独自の自動化デバイス</li> </ul> </div> </div> </blockquote> <div class="row"> <div class="small-12 columns"> <ul class="accordion" data-accordion=""> <li class="accordion-navigation"><a href="#panel1a">次世代シーケンシングへの理解とその専門知識</a> <div id="panel1a" class="content"> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <p><strong>次世代シーケンシング (NGS)</strong> )は、著しいスケールとハイスループットでシーケンシングを行い、1日に数十億もの塩基生成を可能にします。 NGSのハイスループットは迅速でありながら正確で、再現性のあるデータセットを実現し、さらにシーケンシング費用を削減します。 NGSは、ゲノムシーケンシング、ゲノム再シーケンシング、デノボシーケンシング、トランスクリプトームシーケンシング、その他にDNA-タンパク質相互作用の検出やエピゲノムなどを示します。 指数関数的に増加するシーケンシングデータの需要は、計算分析の障害や解釈、データストレージなどの課題を解決します。</p> <p>アプリケーションおよび出発物質に応じて、数百万から数十億の鋳型DNA分子を大規模に並行してシーケンシングすることが可能です。その為に、異なる化学物質を使用するいくつかの市販のNGSプラットフォームを利用することができます。 NGSプラットフォームの種類によっては、事前準備とライブラリー作成が必要です。</p> <p>NGSにとっても、特にデータ処理と分析に関した大きな課題はあります。第3世代技術はゲノミクス研究にさらに革命を起こすであろうと大きく期待されています。</p> </div> </div> <div class="row"> <div class="small-6 medium-6 large-6 columns"> <p><strong>NGS アプリケーション</strong></p> <ul> <li>全ゲノム配列決定</li> <li>デノボシーケンシング</li> <li>標的配列</li> <li>Exomeシーケンシング</li> <li>トランスクリプトーム配列決定</li> <li>ゲノム配列決定</li> <li>ミトコンドリア配列決定</li> <li>DNA-タンパク質相互作用(ChIP-seq</li> <li>バリアント検出</li> <li>ゲノム仕上げ</li> </ul> </div> <div class="small-6 medium-6 large-6 columns"> <p><strong>研究分野におけるNGS:</strong></p> <ul> <li>腫瘍学</li> <li>リプロダクティブ・ヘルス</li> <li>法医学ゲノミクス</li> <li>アグリゲノミックス</li> <li>複雑な病気</li> <li>微生物ゲノミクス</li> <li>食品・環境ゲノミクス</li> <li>創薬ゲノミクス - パーソナライズド・メディカル</li> </ul> </div> <div class="small-12 medium-12 large-12 columns"> <p><strong>NGSの用語</strong></p> <dl> <dt>リード(読み取り)</dt> <dd>この装置から得られた連続した単一のストレッチ</dd> <dt>断片リード</dt> <dd>フラグメントライブラリからの読み込み。 シーケンシングプラットフォームに応じて、読み取りは通常約100〜300bp。</dd> <dt>断片ペアエンドリード</dt> <dd>断片ライブラリーからDNA断片の各末端2つの読み取り。</dd> <dt>メイトペアリード</dt> <dd>大きなDNA断片(通常は予め定義されたサイズ範囲)の各末端から2つの読み取り。</dd> <dt>カバレッジ(例)</dt> <dd>30×適用範囲とは、参照ゲノム中の各塩基対が平均30回の読み取りを示す。</dd> </dl> </div> </div> <div class="row"> <div class="small-12 medium-12 large-12 columns"> <h2>NGSプラットフォーム</h2> <h3><a href="http://www.illumina.com" target="_blank">イルミナ</a></h3> <p>イルミナは、クローン的に増幅された鋳型DNA(クラスター)上に位置する、蛍光標識された可逆的鎖ターミネーターヌクレオチドを用いた配列別合成技術を使用。 DNAクラスターは、ガラスフローセルの表面上に固定化され、 ワークフローは、4つのヌクレオチド(それぞれ異なる蛍光色素で標識された)の組み込み、4色イメージング、色素や末端基の切断、取り込み、イメージングなどを繰り返します。フローセルは大規模な並列配列決定を受ける。 この方法により、単一蛍光標識されたヌクレオチドの制御添加によるモノヌクレオチドのエラーを回避する可能性があります。 読み取りの長さは、通常約100〜150 bpです。</p> <h3><a href="http://www.lifetechnologies.com" target="_blank">イオン トレント</a></h3> <p>イオントレントは、半導体技術チップを用いて、合成中にヌクレオチドを取り込む際に放出されたプロトンを検出します。 これは、イオン球粒子と呼ばれるビーズの表面にエマルションPCR(emPCR)を使用し、リンクされた特定のアダプターを用いてDNA断片を増幅します。 各ビーズは1種類のDNA断片で覆われていて、異なるDNA断片を有するビーズは次いで、チップの陽子感知ウェル内に配置されます。 チップには一度に4つのヌクレオチドのうちの1つが浸水し、このプロセスは異なるヌクレオチドで15秒ごとに繰り返されます。 配列決定の間に4つの塩基の各々が1つずつ導入されます、組み込みの場合はプロトンが放出され、電圧信号が取り込みに比例して検出されます。.</p> <h3><a href="http://www.pacificbiosciences.com" target="_blank">パシフィック バイオサイエンス</a></h3> <p>パシフィックバイオサイエンスでは、20kbを超える塩基対の読み取りも、単一分子リアルタイム(SMRT)シーケンシングによる構造および細胞タイプの変化を観察することができます。 このプラットフォームでは、超長鎖二本鎖DNA(dsDNA)断片が、Megaruptor(登録商標)のようなDiagenode装置を用いたランダムシアリングまたは目的の標的領域の増幅によって生成されます。 SMRTbellライブラリーは、ユニバーサルヘアピンアダプターをDNA断片の各末端に連結することによって生成します。 サイズ選択条件による洗浄ステップの後、配列決定プライマーをSMRTbellテンプレートにアニーリングし、鋳型DNAに結合したDNAポリメラーゼを含む配列決定を、蛍光標識ヌクレオチドの存在下で開始。 各塩基が取り込まれると、異なる蛍光のパルスをリアルタイムで検出します。</p> <h3><a href="https://nanoporetech.com" target="_blank">オックスフォード ナノポア</a></h3> <p>Oxford Nanoporeは、単一のDNA分子配列決定に基づく技術を開発します。その技術により生物学的分子、すなわちDNAが一群の電気抵抗性高分子膜として位置するナノスケールの孔(ナノ細孔)またはその近くを通過し、イオン電流が変化します。 この変化に関する情報は、例えば4つのヌクレオチド(AまたはG r CまたはT)ならびに修飾されたヌクレオチドすべてを区別することによって分子情報に訳されます。 シーケンシングミニオンデバイスのフローセルは、数百個のナノポアチャネルのセンサアレイを含みます。 DNAサンプルは、Diagenode社のMegaruptor(登録商標)を用いてランダムシアリングによって生成され得る超長鎖DNAフラグメントが必要です。</p> <h3><a href="http://www.lifetechnologies.com/be/en/home/life-science/sequencing/next-generation-sequencing/solid-next-generation-sequencing.html" target="_blank">SOLiD</a></h3> <p>SOLiDは、ユニークな化学作用により、何千という個々のDNA分子の同時配列決定を可能にします。 それは、アダプター対ライブラリーのフラグメントが適切で、せん断されたゲノムDNAへのアダプターのライゲーションによるライブラリー作製から始まります。 次のステップでは、エマルジョンPCR(emPCR)を実施して、ビーズの表面上の個々の鋳型DNA分子をクローン的に増幅。 emPCRでは、個々の鋳型DNAをPCR試薬と混合し、水中油型エマルジョン内の疎水性シェルで囲まれた水性液滴内のプライマーコートビーズを、配列決定のためにロードするスライドガラスの表面にランダムに付着。 この技術は、シークエンシングプライマーへのライゲーションで競合する4つの蛍光標識されたジ塩基プローブのセットを使用します。</p> <h3><a href="http://454.com/products/technology.asp" target="_blank">454</a></h3> <p>454は、大規模並列パイロシーケンシングを利用しています。 始めに全ゲノムDNAまたは標的遺伝子断片の300〜800bp断片のライブラリー調製します。 次に、DNAフラグメントへのアダプターの付着および単一のDNA鎖の分離。 その後アダプターに連結されたDNAフラグメントをエマルジョンベースのクローン増幅(emPCR)で処理し、DNAライブラリーフラグメントをミクロンサイズのビーズ上に配置します。 各DNA結合ビーズを光ファイバーチップ上のウェルに入れ、器具に挿入します。 4つのDNAヌクレオチドは、配列決定操作中に固定された順序で連続して加えられ、並行して配列決定されます。</p> </div> </div> </div> </li> </ul> </div> </div>', 'in_footer' => true, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'next-generation-sequencing', 'meta_keywords' => 'Next-generation sequencing,NGS,Whole genome sequencing,NGS platforms,DNA/RNA shearing', 'meta_description' => 'Diagenode offers kits and DNA/RNA shearing technology prior to next-generation sequencing, many Next-generation sequencing platforms require preparation of the DNA.', 'meta_title' => 'Next-generation sequencing (NGS) Applications and Methods | Diagenode', 'modified' => '2018-07-26 05:24:29', 'created' => '2015-04-01 22:47:04', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '13', 'position' => '10', 'parent_id' => '3', 'name' => 'DNA/RNA shearing', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns">In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critical aspects of optimal library preparation is the quality of the DNA to be sequenced. The DNA must first be effectively and consistently sheared into the appropriate fragment size (depending on the sequencing platform) to enable sensitive and reliable NGS results. The <strong>Bioruptor</strong><sup>®</sup> <strong>Pico</strong> and the <strong>Megaruptor</strong><sup>®</sup> provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows. Follow our guidelines and find the good parameters for your expected DNA size: <a href="https://pybrevet.typeform.com/to/o8cQfM">DNA shearing with the Bioruptor<sup>®</sup></a>.</div> </div> <p></p> <div class="row"> <div class="small-7 medium-7 large-7 columns text-center"><img src="https://www.diagenode.com/img/applications/true-flexibility-with-br-ngs.jpg" /></div> <div class="small-5 medium-5 large-5 columns"><small><strong>Programmable DNA size distribution and high reproducibility with Bioruptor<sup>®</sup> Pico using 0.65 (panel A) or 0.1 ml (panel B) microtubes</strong>. <b>Panel A:</b> 200 bp after 13 cycles (13 sec ON/OFF) using 100 µl volume. Average size: 204; CV%:1.89%). <b>Panel B:</b> 200 bp after 20 cycles (30 sec ON/OFF) using 10 µl volume. (Average size: 215 bp; CV%: 6.6%). <b>Panel A & B:</b> peak electropherogram view. <b>Panel C & D:</b> virtual gel view.</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-10 medium-10 large-10 columns text-center end small-offset-1"><img src="https://www.diagenode.com/img/applications/megaruptor-short-frag.jpg" /></div> <div class="small-12 medium-12 large-12 columns"><small><strong> Reproducible and narrow DNA size distribution with Megaruptor® using short fragment size Hydropores Validation using two different DNA sources and two different methods of analysis. A:</strong> Shearing of lambda phage genomic DNA (20 ng/μl; 150 μl/sample) sheared at different speed settings and analyzed on 1% agarose gel. <strong>B:</strong> Bioanalyzer profiles of human genomic DNA (20 ng/μl; 150 μl/sample) sheared at different software settings of 2 and 5 kb. Three independent experiments were run for each setting. (Agilent DNA 12000 kit was used for separation and fragment sizing).</small></div> </div> <p><br /><br /></p> <div class="row"> <div class="small-4 medium-4 large-4 columns text-center"><img src="https://www.diagenode.com/img/applications/megaruptor-long-frag.jpg" /></div> <div class="small-8 medium-8 large-8 columns"><small><strong> Demonstrated shearing to fragment sizes between 15 kb and 75 kb with Megaruptor® using long fragment size Hydropores. </strong>Image shows DNA size distribution of human genomic DNA sheared with long fragment Hydropores. DNA was analyzed by pulsed field gel electrophoresis (PFGE) in 1% agarose gel and a mean size of smears was estimated using Image Lab 4.1 software.<br /> * indicates unsheared DNA </small></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'dna-rna-shearing', 'meta_keywords' => 'DNA/RNA shearing,Bioruptor® Pico,Megaruptor®,Next-Generation Sequencing ', 'meta_description' => 'Bioruptor® Pico and the Megaruptor® provide superior sample yields, fragment size, and consistency, which are essential for Next-Generation Sequencing workflows.', 'meta_title' => 'DNA shearing & RNA shearing for Next-Generation Sequencing (NGS) | Diagenode', 'modified' => '2017-12-08 14:44:11', 'created' => '2014-10-29 12:45:41', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '17', 'position' => '10', 'parent_id' => '4', 'name' => 'Protein extraction', 'description' => '<div class="row"> <div class="large-12 columns">Various biochemical and analytical techniques require the extraction of protein from tissues or mammalian, yeast and bacterial cells. Obtaining high quality and yields of proteins is important for further downstream protein characterization such as in PAGE, western blotting, mass spectrometry or protein purification. The efficient disruption and homogenization of tissues and cultured cells obtained in just one step using <strong>Diagenode's Bioruptor</strong><sup>®</sup> deliver high quality protein.</div> </div> <p></p> <div class="row"> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_standard_plus.png" /> <p><small>Western blot analysis of GAPDH and HSP90 proteins in tissues (various mouse tissues) and cultured cell extracts (HeLA).</small></p> </div> <div class="small-6 medium-6 large-6 columns text-center"><img src="https://www.diagenode.com/img/applications/protein_extraction_pico.png" /> <p><small>Western blot analysis of GAPDH and ß-tubulin proteins in tissues (mouse liver) and cultured cell extracts (HeLA).</small></p> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'protein-extraction', 'meta_keywords' => 'Protein extraction,Bioruptor,Sonication,Protein Analysis', 'meta_description' => 'Diagenode provides efficient disruption and homogenization of tissues and cultured cells obtained in just one step using Bioruptor® deliver high quality protein.', 'meta_title' => 'Protein extraction using Bioruptor® Sonication device | Diagenode', 'modified' => '2017-10-16 14:39:42', 'created' => '2014-07-02 04:41:03', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '6', 'position' => '10', 'parent_id' => '1', 'name' => 'メチル化DNA結合タンパク質', 'description' => '<div class="row"> <div class="large-12 columns">MBD方法は、メチル化DNAに対するH6-GST-MBD融合タンパク質の非常に高い親和性に基づいています。 このタンパク質は、N末端His6タグを含むグルタチオン-S-トランスフェラーゼ(GST)とのC末端融合物として、ヒトMeCP2のメチル結合ドメイン(MBD)を含有します。 このH6-GST-MBD融合タンパク質を用いて、メチル化CpGを含むDNAを特異的に単離する事が可能です。<br /><br />DiagenodeのMethylCap®キットは、二本鎖DNAの高濃縮と、メチル化CpG密度の関数における微分分画を可能にします。 分画はサンプルの複雑さを軽減し、次世代のシーケンシングを容易にします。 MethylCapアッセイに先立ち、DNAを最初に抽出し、Picoruptorソニケーターを用いて断片化します。<br /> <h3>概要</h3> <p class="text-center"><br /><img src="https://www.diagenode.com/img/applications/methyl_binding_domain_overview.jpg" /></p> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'methylbinding-domain-protein', 'meta_keywords' => 'Epigenetic,Methylbinding Domain Protein,MBD,DNA methylation,DNA replication,MethylCap,MethylCap assay,', 'meta_description' => 'Methylbinding Domain Protein(MBD) approach is based on the very high affinity of a H6-GST-MBD fusion protein for methylated DNA. This protein consists of the methyl binding domain (MBD) of human MeCP2, as a C-terminal fusion with Glutathione-S-transferase', 'meta_title' => 'Epigenetic Methylbinding Domain Protein(MBD) - DNA methylation | Diagenode', 'modified' => '2019-03-22 12:32:12', 'created' => '2015-06-02 17:05:42', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '9', 'position' => '10', 'parent_id' => '2', 'name' => 'ChIP-seq', 'description' => '<div class="row"> <div class="large-12 columns">Chromatin Immunoprecipitation (ChIP) coupled with high-throughput massively parallel sequencing as a detection method (ChIP-seq) has become one of the primary methods for epigenomics researchers, namely to investigate protein-DNA interaction on a genome-wide scale. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</div> <div class="large-12 columns"></div> <h5 class="large-12 columns"><strong></strong></h5> <h5 class="large-12 columns"><strong>The ChIP-seq workflow</strong></h5> <div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/chip-seq-diagram.png" /></div> <div class="large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>Crosslink chromatin-bound proteins (histones or transcription factors) to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing:</strong> Fragment chromatin by sonication to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: Capture protein-DNA complexes with <strong><a href="../categories/chip-seq-grade-antibodies">specific ChIP-seq grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: Reverse cross-links, elute, and purify </li> <li class="large-12 columns"><strong>NGS Library Preparation</strong>: Ligate adapters and amplify IP'd material</li> <li class="large-12 columns"><strong>Bioinformatic analysis</strong>: Perform r<span style="font-weight: 400;">ead filtering and trimming</span>, r<span style="font-weight: 400;">ead specific alignment, enrichment specific peak calling, QC metrics, multi-sample cross-comparison etc. </span></li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="../pages/which-kit-to-choose"><img alt="" src="https://www.diagenode.com/img/banners/banner-decide.png" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="../pages/chip-kit-customizer-1"><img alt="" src="https://www.diagenode.com/img/banners/banner-customizer.png" /></a></div> </div> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chromatin-immunoprecipitation-sequencing', 'meta_keywords' => 'Chromatin Immunoprecipitation Sequencing,ChIP-Seq,ChIP-seq grade antibodies,DNA purification,qPCR,Shearing of chromatin', 'meta_description' => 'Diagenode offers wide range of kits and antibodies for Chromatin Immunoprecipitation Sequencing (ChIP-Seq) and also provides Bioruptor for chromatin shearing', 'meta_title' => 'Chromatin Immunoprecipitation - ChIP-seq Kits - Dna methylation | Diagenode', 'modified' => '2017-11-14 09:57:16', 'created' => '2015-04-12 18:08:46', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '11', 'position' => '10', 'parent_id' => '3', 'name' => 'FFPE DNA extraction', 'description' => '<div class="row"> <div class="large-12 columns">Diagenode's high yields FFPE DNA extraction using Bioruptor<sup><span>®</span></sup> is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no toxic reagents, digest tissues, and purify DNA with high yields and low sample degradation. The DNA can then be analyzed by traditional methods or can be sheared with the Bioruptor<sup>®</sup> Pico ultrasonicator for downstream NGS library prep using the MicroPlex Library Preparation Kit.</div> <div class="small-12 medium-12 large-12 columns text-center"><img src="https://www.diagenode.com/img/applications/ffpe_workflow.png" /></div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'slug' => 'ffpe-dna-extraction', 'meta_keywords' => 'FFPE DNA extraction,Next-Gen Sequencing,Bioruptor® ultrasonicator', 'meta_description' => 'Diagenode's high yields FFPE DNA extraction using Bioruptor is a superior method for extracting DNA for Next-Gen Sequencing. Our FFPE DNA Extraction kit contains optimized reagents that are added directly to the FFPE samples to remove paraffin with no tox', 'meta_title' => 'FFPE DNA extraction using Bioruptor® ultrasonicator | Diagenode', 'modified' => '2017-10-16 14:34:57', 'created' => '2014-10-01 01:24:40', 'ProductsApplication' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '10', 'position' => '10', 'parent_id' => '2', 'name' => 'ChIP-qPCR', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns text-justify"> <p class="text-justify">Chromatin Immunoprecipitation (ChIP) coupled with quantitative PCR can be used to investigate protein-DNA interaction at known genomic binding sites. if sites are not known, qPCR primers can also be designed against potential regulatory regions such as promoters. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of performing real-time PCR is minimal. This technique is now used in a variety of life science disciplines including cellular differentiation, tumor suppressor gene silencing, and the effect of histone modifications on gene expression.</p> <p class="text-justify"><strong>The ChIP-qPCR workflow</strong></p> </div> <div class="small-12 medium-12 large-12 columns text-center"><br /> <img src="https://www.diagenode.com/img/chip-qpcr-diagram.png" /></div> <div class="small-12 medium-12 large-12 columns"><br /> <ol> <li class="large-12 columns"><strong>Chromatin preparation: </strong>cell fixation (cross-linking) of chromatin-bound proteins such as histones or transcription factors to DNA followed by cell lysis.</li> <li class="large-12 columns"><strong>Chromatin shearing: </strong>fragmentation of chromatin<strong> </strong>by sonication down to desired fragment size (100-500 bp)</li> <li class="large-12 columns"><strong>Chromatin IP</strong>: protein-DNA complexe capture using<strong> <a href="https://www.diagenode.com/en/categories/chip-grade-antibodies">specific ChIP-grade antibodies</a></strong> against the histone or transcription factor of interest</li> <li class="large-12 columns"><strong>DNA purification</strong>: chromatin reverse cross-linking and elution followed by purification<strong> </strong></li> <li class="large-12 columns"><strong>qPCR and analysis</strong>: using previously designed primers to amplify IP'd material at specific loci</li> </ol> </div> </div> <div class="row" style="margin-top: 32px;"> <div class="small-12 medium-10 large-9 small-centered columns"> <div class="radius panel" style="background-color: #fff;"> <h3 class="text-center" style="color: #b21329;">Need guidance?</h3> <p class="text-justify">Choose our full ChIP kits or simply choose what you need from antibodies, buffers, beads, chromatin shearing and purification reagents. With the ChIP Kit Customizer, you have complete flexibility on which components you want from our validated ChIP kits.</p> <div class="row"> <div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/which-kit-to-choose"><img src="https://www.diagenode.com/img/banners/banner-decide.png" alt="" /></a></div> <div class="small-6 medium-6 large-6 columns"><a href="https://www.diagenode.com/pages/chip-kit-customizer-1"><img src="https://www.diagenode.com/img/banners/banner-customizer.png" alt="" /></a></div> </div> </div> </div> </div>', 'in_footer' => false, 'in_menu' => true, 'online' => true, 'tabular' => true, 'slug' => 'chip-qpcr', 'meta_keywords' => 'Chromatin immunoprecipitation,ChIP Quantitative PCR,polymerase chain reaction (PCR)', 'meta_description' => 'Diagenode's ChIP qPCR kits can be used to quantify enriched DNA after chromatin immunoprecipitation. ChIP-qPCR is advantageous in studies that focus on specific genes and potential regulatory regions across differing experimental conditions as the cost of', 'meta_title' => 'ChIP Quantitative PCR (ChIP-qPCR) | Diagenode', 'modified' => '2018-01-09 16:46:56', 'created' => '2014-12-11 00:22:08', 'ProductsApplication' => array( [maximum depth reached] ) ) ), 'Category' => array( (int) 0 => array( 'id' => '6', 'position' => '1', 'parent_id' => '1', 'name' => 'Bioruptor<sup>®</sup>', 'description' => '<div class="row"> <div class="small-12 medium-12 large-12 columns"><br /> <p><span>Diagenode focuses on state-of-the-art preparation of high quality biological and chemical samples by developing the industry’s most advanced water bath sonicators and hydrodynamic devices. Our instruments are ideal for a number of applications in various fields of studies including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</span></p> <p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor/TAB-BR-comparaison.pdf" target="_blank"><img src="https://www.diagenode.com/img/bouton-comparaison.png" /></a></p> </div> <!-- <center> <div class="small-12 medium-4 large-4 columns"> <script>// <![CDATA[ var date = new Date(); var heure = date.getHours(); var jour = date.getDay(); var semaine = Math.floor(date.getDate() / 7) + 1; if (jour === 2 && ( (heure >= 9 && heure < 9.5) || (heure >= 18 && heure < 18.5) )) { document.write('<a href="https://us02web.zoom.us/j/85467619762"><img src="https://www.diagenode.com/img/epicafe-ON.gif"></a>'); } else { document.write('<a href="https://go.diagenode.com/l/928883/2023-04-26/3kq1v"><img src="https://www.diagenode.com/img/epicafe-OFF.png"></a>'); } // ]]></script> </div> </center></div> <p><span></span></p> <div class="content_block"> <div class="centered row"> <h2 style="text-align: center;"></h2> <h2 style="text-align: center;">Technology explained</h2> <div class="container-wrapper-genially" style="position: relative; min-height: 400px; max-width: 80%; margin: 0 auto;"><video width="300" height="150" style="position: absolute; top: 45%; left: 50%; transform: translate(-50%, -50%); width: 80px; height: 80px; margin-bottom: 10%;" class="loader-genially" autoplay="autoplay" loop="loop" playsinline="playsInline" muted="muted"><source src="https://static.genial.ly/resources/panel-loader-low.mp4" type="video/mp4" />Your browser does not support the video tag.</video> <div id="601970a2edea170d2af29118" class="genially-embed" style="margin: 0px auto; position: relative; height: auto; width: 100%;"></div> </div> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script async="" src="https://edge.fullstory.com/s/fs.js" crossorigin="anonymous"></script> <script>// <![CDATA[ (function (d) { var js, id = "genially-embed-js", ref = d.getElementsByTagName("script")[0]; if (d.getElementById(id)) { return; } js = d.createElement("script"); js.id = id; js.async = true; js.src = "https://view.genial.ly/static/embed/embed.js"; ref.parentNode.insertBefore(js, ref); }(document)); // ]]></script> </div> </div>--> <p><span> <br /></span></p> <div class="spacer"></div> <div class="content_block"> <div class="centered row"> <h2 style="text-align: center;">Reproductibility is our priority</h2> </div> </div> <div><img src="https://www.diagenode.com/img/shearing/reproductibility.png" alt="reproductibility" /> <p class="bottom_note"></p> </div> <div class="content_block"> <div class="centered row"> <h2 style="text-align: center;">An affordable instrument for wide range of applications</h2> </div> </div> <p style="text-align: center;">Designed for any researchers, the Bioruptor gives the user the right level of flexibility.</p> <table style="width: 972px;"> <tbody> <tr style="height: 56px;"> <th style="width: 380px; height: 56px;"></th> <th class="text-center" style="width: 126px; height: 56px;">Bioruptor</th> <th class="text-center" style="width: 141px; height: 56px;">Cup Horn Sonicators</th> <th class="text-center" style="width: 156px; height: 56px;">Focused <br />ultra-sonicators</th> <th class="text-center" style="width: 155px; height: 56px;">Multi Sample Sonicator</th> </tr> <tr style="height: 38px;"> <td style="width: 380px; height: 38px;">Instrument pricing</td> <td style="width: 126px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td> <td style="width: 141px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td> <td style="width: 156px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td> <td style="width: 155px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td> </tr> <tr style="height: 38px;"> <td style="width: 380px; height: 38px;">Consumables pricing</td> <td style="width: 126px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td> <td style="width: 141px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i></center></td> <td style="width: 156px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td> <td style="width: 155px; height: 38px;"><center><i class="fa fa-usd" aria-hidden="true"></i><i class="fa fa-usd" aria-hidden="true"></i></center></td> </tr> <tr style="height: 38px;"> <td style="width: 380px; height: 38px;">Range of applications</td> <td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> </tr> <tr style="height: 38px;"> <td style="width: 380px; height: 38px;">Scalable and sample volume flexibility</td> <td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i></center></td> </tr> <tr style="height: 38px;"> <td style="width: 380px; height: 38px;">Throughput</td> <td style="width: 126px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 141px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 156px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> <td style="width: 155px; height: 38px;"><center><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i><i class="fa fa-flask" aria-hidden="true"></i></center></td> </tr> </tbody> </table> <div class="content_block"> <div class="centered row"> <h2 style="text-align: center;"></h2> <h2 style="text-align: center;">Bioruptor ultrasonication for best results in:</h2> <p><b><span>✓ Chromatin shearing</span><span> </span><span style="font-weight: 400;">- Industry leader in accurate and tight fragment ranges</span></b></p> <p><b><span>✓ DNA shearing</span><span> </span><span style="font-weight: 400;">- Excellent results for optimal fragment lengths in NGS library prep</span></b></p> <p><b><span>✓<span> </span></span>Protein aggregation studies </b><span style="font-weight: 400;">- Standardizing seeding with the robust Bioruptor.<br /></span><i><span style="font-weight: 400;">Read the app note by Dr. Kelvin Luk at the University of Pennsylvania </span></i><a href="https://www.diagenode.com/en/documents/standardizing-seeding-experiments-for-the-understanding-of-parkinson-disease" style="color: #13b29c;"><i><span style="font-weight: 400;">“Standardizing seeding experiments using the Bioruptor® for the understanding of the neuronal alpha-synuclein pathology”</span></i></a></p> <p><b><b><span>✓<span> </span></span></b>3D genome analysis with Hi-C</b><span style="font-weight: 400;"> - Preparing chromatin libraries with high-quality sonication.<br /></span><i><span style="font-weight: 400;">Read the app note, “</span></i><a href="https://www.diagenode.com/en/documents/applicationnote-arima-low-input" style="color: #13b29c;"><i><span style="font-weight: 400;">Unlock Low-Input 3D Genome Analysis with the Arima-HiC Kit”</span></i></a></p> <p><b><b><span>✓<span> </span></span></b>Mass spectrometry</b> <b>and increasing protein identification</b><span style="font-weight: 400;">- Sample preparation using Preomics iST and Bioruptor sonication.<br /></span><i><span style="font-weight: 400;">Read the app note “</span></i><a href="https://www.diagenode.com/en/documents/wp-ist-adaptators" style="color: #13b29c;"><i><span style="font-weight: 400;">Increase your iST ultrasonication throughput with the new Bioruptor® Pico cartridge holder”</span></i></a></p> <p><i><span style="font-weight: 400;"><b><span>✓ </span></b></span></i><span style="font-weight: 400;"><b>Cell lysis, liposome prep, protein extraction, RNA extraction and more</b></span></p> <p><i><span style="font-weight: 400;"><b><span>✓ </span></b></span></i><span style="font-weight: 400;"><b>CUT&RUN –Sonication of input DNA (for enrichment comparison) for NGS</b></span></p> </div> </div> <p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p> <p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p> </div>', 'no_promo' => false, 'in_menu' => true, 'online' => true, 'tabular' => false, 'hide' => false, 'all_format' => false, 'is_antibody' => false, 'slug' => 'bioruptor-shearing-device', 'cookies_tag_id' => null, 'meta_keywords' => 'Bioruptor,ultrasonicator devices,probe sonicator,Next-Generation Sequencing', 'meta_description' => 'Bioruptor Sonication is ideal for Chromatin Shearing for Chromatin Immunoprecipitation (ChIP), Genomic DNA Shearing for next Generation Sequencing, RNA Shearing, Cell and Tissue Disruption', 'meta_title' => 'Bioruptor Sonication for Chromatin, DNA / RNA Shearing, Cell and Tissue Disruption | Diagenode', 'modified' => '2024-08-28 14:03:21', 'created' => '2014-12-18 22:08:39', 'ProductsCategory' => array( [maximum depth reached] ), 'CookiesTag' => array([maximum depth reached]) ) ), 'Document' => array( (int) 0 => array( 'id' => '1067', 'name' => 'Chromatin Shearing Guide', 'description' => '<p>Guide for successful chromatin preparation using the Bioruptor Pico</p>', 'image_id' => null, 'type' => 'Protocol', 'url' => 'files/products/shearing_technology/bioruptor/chromatin-shearing-guide-Pico.pdf', 'slug' => 'chromatin-shearing-guide-pico', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-01-21 15:27:22', 'created' => '2020-01-21 15:27:22', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1068', 'name' => 'DNA shearing guide', 'description' => '<p>DNA shearing for Next-Generation Sequencing with the Bioruptor Pico</p>', 'image_id' => null, 'type' => 'Protocol', 'url' => 'files/protocols/protocol-dna-shearing-bioruptor-pico.pdf', 'slug' => 'protocol-dna-shearing-bioruptor-pico', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-08-11 10:13:24', 'created' => '2020-01-21 15:30:32', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '1072', 'name' => 'Which tubes for Bioruptor<sup>®</sup> Pico', 'description' => '', 'image_id' => null, 'type' => 'Document', 'url' => 'files/products/shearing_technology/bioruptor/org-tubes-pico-01_20.pdf', 'slug' => 'org-tubes-pico-01-20', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-02-10 11:16:30', 'created' => '2020-02-10 10:55:30', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '1127', 'name' => 'Unlock Low-Input 3D Genome Analysis with the Arima-HiC Kit', 'description' => '<p><span>By combining the optimized chemistry of the Arima-HiC kit along with new low input protocols, the potential applications of powerful Hi-C technology are unlocked. When studying samples that are difficult to obtain or grow, low input solutions can help you understand genome structure across a new range of low input samples. In addition, the Diagenode Bioruptor Pico assures that chromatin is sheared to optimal fragment lengths.</span></p>', 'image_id' => '247', 'type' => 'Application Note', 'url' => 'files/application_notes/ApplicationNote-Arima-Low-Input.pdf', 'slug' => 'applicationnote-arima-low-input', 'meta_keywords' => 'application note arima low input', 'meta_description' => 'application note arima low input', 'modified' => '2021-02-09 09:55:59', 'created' => '2021-02-09 09:55:59', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1074', 'name' => 'Datasheet of Bioruptor tubes', 'description' => '<p>Datasheet of Diagenode tubes for Bioruptor Pico and Bioruptor Plus.</p>', 'image_id' => null, 'type' => 'Datasheet', 'url' => 'files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf', 'slug' => 'tds-bioruptor-tubes', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2022-02-23 12:21:44', 'created' => '2020-03-23 10:41:46', 'ProductsDocument' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '1170', 'name' => 'Critical steps for Bioruptor® maintenance and efficient shearing', 'description' => '', 'image_id' => null, 'type' => 'Document', 'url' => 'files/products/shearing_technology/critical-steps-bioruptor-web.pdf', 'slug' => 'critical-steps-bioruptor-maintenance', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2023-08-31 14:27:41', 'created' => '2023-08-31 14:27:41', 'ProductsDocument' => array( [maximum depth reached] ) ) ), 'Feature' => array( (int) 0 => array( 'id' => '6', 'name' => 'All-in-one solution', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 11:50:41', 'created' => '2014-06-21 12:07:09', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '16', 'name' => 'Highly reproducible', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-11-09 14:21:15', 'created' => '2015-05-11 05:24:25', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '7', 'name' => 'Processing of 6-16 samples', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2018-03-13 11:10:21', 'created' => '2014-11-09 09:21:21', 'ProductsFeature' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '1', 'name' => 'User friendly software', 'slug' => '', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2015-07-24 17:39:16', 'created' => '2014-06-27 10:32:35', 'ProductsFeature' => array( [maximum depth reached] ) ) ), 'Image' => array( (int) 0 => array( 'id' => '1803', 'name' => 'product/shearing_technologies/B01080000-1.jpg', 'alt' => 'Bioruptor Pico', 'modified' => '2020-01-10 10:55:07', 'created' => '2020-01-10 10:52:54', 'ProductsImage' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '1804', 'name' => 'product/shearing_technologies/B01080000-2.jpg', 'alt' => 'Bioruptor Pico', 'modified' => '2020-01-10 10:53:08', 'created' => '2020-01-10 10:53:08', 'ProductsImage' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '1805', 'name' => 'product/shearing_technologies/B01080000-3.jpg', 'alt' => 'Bioruptor Pico', 'modified' => '2020-01-10 10:53:46', 'created' => '2020-01-10 10:53:46', 'ProductsImage' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '1806', 'name' => 'product/shearing_technologies/B01080000-4.jpg', 'alt' => 'Bioruptor Pico', 'modified' => '2020-01-10 10:53:56', 'created' => '2020-01-10 10:53:56', 'ProductsImage' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '1807', 'name' => 'product/shearing_technologies/B01080000-5.jpg', 'alt' => 'Bioruptor Pico', 'modified' => '2020-01-10 10:54:06', 'created' => '2020-01-10 10:54:06', 'ProductsImage' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '1772', 'name' => 'product/shearing_technologies/B010600010.jpg', 'alt' => 'B010600010', 'modified' => '2018-02-14 15:41:46', 'created' => '2018-02-14 15:41:46', 'ProductsImage' => array( [maximum depth reached] ) ) ), 'Promotion' => array(), 'Protocol' => array( (int) 0 => array( 'id' => '73', 'name' => 'DNA shearing guide', 'description' => '<p>DNA shearing for Next-Generation Sequencing with the Bioruptor Pico</p>', 'image_id' => null, 'type' => 'Protocol', 'url' => 'files/protocols/protocol-dna-shearing-bioruptor-pico.pdf', 'slug' => 'protocol-dna-shearing-bioruptor-pico', 'meta_keywords' => '', 'meta_description' => '', 'modified' => '2020-08-11 10:16:00', 'created' => '0000-00-00 00:00:00', 'ProductsProtocol' => array( [maximum depth reached] ) ) ), 'Publication' => array( (int) 0 => array( 'id' => '4881', 'name' => 'LEO1 Is Required for Efficient Entry into Quiescence, Control of H3K9 Methylation and Gene Expression in Human Fibroblasts', 'authors' => 'Laurent M. et al.', 'description' => '<p><span>(1) Background: The LEO1 (Left open reading frame 1) protein is a conserved subunit of the PAF1C complex (RNA polymerase II-associated factor 1 complex). PAF1C has well-established mechanistic functions in elongation of transcription and RNA processing. We previously showed, in fission yeast, that LEO1 controls histone H3K9 methylation levels by affecting the turnover of histone H3 in chromatin, and that it is essential for the proper regulation of gene expression during cellular quiescence. Human fibroblasts enter a reversible quiescence state upon serum deprivation in the growth media. Here we investigate the function of LEO1 in human fibroblasts. (2) Methods: We knocked out the </span><span class="html-italic">LEO1</span><span><span> </span>gene using CRISPR/Cas9 methodology in human fibroblasts and verified that the LEO1 protein was undetectable by Western blot. We characterized the phenotype of the<span> </span></span><span class="html-italic">ΔLEO1</span><span><span> </span>knockout cells with FACS analysis and cell growth assays. We used RNA-sequencing using spike-in controls to measure gene expression and spike-in controlled ChIP-sequencing experiments to measure the histone modification H3K9me2 genome-wide. (3) Results: Gene expression levels are altered in quiescent cells, however factors controlling chromatin and gene expression changes in quiescent human cells are largely unknown. The<span> </span></span><span class="html-italic">ΔLEO1</span><span><span> </span>knockout fibroblasts are viable but have reduced metabolic activity compared to wild-type cells.<span> </span></span><span class="html-italic">ΔLEO1</span><span><span> </span>cells showed a slower entry into quiescence and a different morphology compared to wild-type cells. Gene expression was generally reduced in quiescent wild-type cells. The downregulated genes included genes involved in cell proliferation. A small number of genes were upregulated in quiescent wild-type cells including several genes involved in ERK1/ERK2 and Wnt signaling. In quiescent<span> </span></span><span class="html-italic">ΔLEO1</span><span><span> </span>cells, many genes were mis-regulated compared to wild-type cells. This included genes involved in Calcium ion transport and cell morphogenesis. Finally, spike-in controlled ChIP-sequencing experiments demonstrated that the histone modification H3K9me2 levels are globally increased in quiescent<span> </span></span><span class="html-italic">ΔLEO1</span><span><span> </span>cells. (4) Conclusions: Thus, LEO1 is important for proper entry into cellular quiescence, control of H3K9me2 levels, and gene expression in human fibroblasts.</span></p>', 'date' => '2023-11-17', 'pmid' => 'https://www.mdpi.com/2218-273X/13/11/1662', 'doi' => 'https://doi.org/10.3390/biom13111662', 'modified' => '2023-11-21 12:01:53', 'created' => '2023-11-21 12:01:53', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 1 => array( 'id' => '4845', 'name' => 'DeSUMOylation of chromatin-bound proteins limits the rapidtranscriptional reprogramming induced by daunorubicin in acute myeloidleukemias.', 'authors' => 'Boulanger M. et al.', 'description' => '<p>Genotoxicants have been used for decades as front-line therapies against cancer on the basis of their DNA-damaging actions. However, some of their non-DNA-damaging effects are also instrumental for killing dividing cells. We report here that the anthracycline Daunorubicin (DNR), one of the main drugs used to treat Acute Myeloid Leukemia (AML), induces rapid (3 h) and broad transcriptional changes in AML cells. The regulated genes are particularly enriched in genes controlling cell proliferation and death, as well as inflammation and immunity. These transcriptional changes are preceded by DNR-dependent deSUMOylation of chromatin proteins, in particular at active promoters and enhancers. Surprisingly, inhibition of SUMOylation with ML-792 (SUMO E1 inhibitor), dampens DNR-induced transcriptional reprogramming. Quantitative proteomics shows that the proteins deSUMOylated in response to DNR are mostly transcription factors, transcriptional co-regulators and chromatin organizers. Among them, the CCCTC-binding factor CTCF is highly enriched at SUMO-binding sites found in cis-regulatory regions. This is notably the case at the promoter of the DNR-induced NFKB2 gene. DNR leads to a reconfiguration of chromatin loops engaging CTCF- and SUMO-bound NFKB2 promoter with a distal cis-regulatory region and inhibition of SUMOylation with ML-792 prevents these changes.</p>', 'date' => '2023-07-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37462077', 'doi' => '10.1093/nar/gkad581', 'modified' => '2023-08-01 14:16:43', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 2 => array( 'id' => '4846', 'name' => 'RNA polymerase II CTD is dispensable for transcription and requiredfor termination in human cells.', 'authors' => 'Yahia Y. et al.', 'description' => '<p>The largest subunit of RNA polymerase (Pol) II harbors an evolutionarily conserved C-terminal domain (CTD), composed of heptapeptide repeats, central to the transcriptional process. Here, we analyze the transcriptional phenotypes of a CTD-Δ5 mutant that carries a large CTD truncation in human cells. Our data show that this mutant can transcribe genes in living cells but displays a pervasive phenotype with impaired termination, similar to but more severe than previously characterized mutations of CTD tyrosine residues. The CTD-Δ5 mutant does not interact with the Mediator and Integrator complexes involved in the activation of transcription and processing of RNAs. Examination of long-distance interactions and CTCF-binding patterns in CTD-Δ5 mutant cells reveals no changes in TAD domains or borders. Our data demonstrate that the CTD is largely dispensable for the act of transcription in living cells. We propose a model in which CTD-depleted Pol II has a lower entry rate onto DNA but becomes pervasive once engaged in transcription, resulting in a defect in termination.</p>', 'date' => '2023-07-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37424514', 'doi' => '10.15252/embr.202256150', 'modified' => '2023-08-01 14:17:54', 'created' => '2023-08-01 15:59:38', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 3 => array( 'id' => '4793', 'name' => 'Targeting lymphoid-derived IL-17 signaling to delay skin aging.', 'authors' => 'Paloma S. et al.', 'description' => '<p><span>Skin aging is characterized by structural and functional changes that contribute to age-associated frailty. This probably depends on synergy between alterations in the local niche and stem cell-intrinsic changes, underscored by proinflammatory microenvironments that drive pleotropic changes. The nature of these age-associated inflammatory cues, or how they affect tissue aging, is unknown. Based on single-cell RNA sequencing of the dermal compartment of mouse skin, we show a skew towards an IL-17-expressing phenotype of T helper cells, γδ T cells and innate lymphoid cells in aged skin. Importantly, in vivo blockade of IL-17 signaling during aging reduces the proinflammatory state of the skin, delaying the appearance of age-related traits. Mechanistically, aberrant IL-17 signals through NF-κB in epidermal cells to impair homeostatic functions while promoting an inflammatory state. Our results indicate that aged skin shows signs of chronic inflammation and that increased IL-17 signaling could be targeted to prevent age-associated skin ailments.</span></p>', 'date' => '2023-06-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37291218', 'doi' => '10.1038/s43587-023-00431-z', 'modified' => '2023-06-14 15:56:56', 'created' => '2023-06-13 21:11:31', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 4 => array( 'id' => '4796', 'name' => 'Nicotinamide N-methyltransferase sustains a core epigenetic programthat promotes metastatic colonization in breast cancer.', 'authors' => 'Couto J.P. et al.', 'description' => '<p><span>Metastatic colonization of distant organs accounts for over 90% of deaths related to solid cancers, yet the molecular determinants of metastasis remain poorly understood. Here, we unveil a mechanism of colonization in the aggressive basal-like subtype of breast cancer that is driven by the NAD</span><sup>+</sup><span><span> </span>metabolic enzyme nicotinamide N-methyltransferase (NNMT). We demonstrate that NNMT imprints a basal genetic program into cancer cells, enhancing their plasticity. In line, NNMT expression is associated with poor clinical outcomes in patients with breast cancer. Accordingly, ablation of NNMT dramatically suppresses metastasis formation in pre-clinical mouse models. Mechanistically, NNMT depletion results in a methyl overflow that increases histone H3K9 trimethylation (H3K9me3) and DNA methylation at the promoters of PR/SET Domain-5 (PRDM5) and extracellular matrix-related genes. PRDM5 emerged in this study as a pro-metastatic gene acting via induction of cancer-cell intrinsic transcription of collagens. Depletion of PRDM5 in tumor cells decreases COL1A1 deposition and impairs metastatic colonization of the lungs. These findings reveal a critical activity of the NNMT-PRDM5-COL1A1 axis for cancer cell plasticity and metastasis in basal-like breast cancer.</span></p>', 'date' => '2023-06-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37259596', 'doi' => '10.15252/embj.2022112559', 'modified' => '2023-06-15 08:35:19', 'created' => '2023-06-13 21:11:31', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 5 => array( 'id' => '4812', 'name' => 'SOX expression in prostate cancer drives resistance to nuclear hormonereceptor signaling inhibition through the WEE1/CDK1 signaling axis.', 'authors' => 'Williams A. et al.', 'description' => '<p><span>The development of androgen receptor signaling inhibitor (ARSI) drug resistance in prostate cancer (PC) remains therapeutically challenging. Our group has described the role of sex determining region Y-box 2 (SOX2) overexpression in ARSI-resistant PC. Continuing this work, we report that NR3C1, the gene encoding glucocorticoid receptor (GR), is a novel SOX2 target in PC, positively regulating its expression. Similar to ARSI treatment, SOX2-positive PC cells are insensitive to GR signaling inhibition using a GR modulating therapy. To understand SOX2-mediated nuclear hormone receptor signaling inhibitor (NHRSI) insensitivity, we performed RNA-seq in SOX2-positive and -negative PC cells following NHRSI treatment. RNA-seq prioritized differentially regulated genes mediating the cell cycle, including G2 checkpoint WEE1 Kinase (WEE1) and cyclin-dependent kinase 1 (CDK1). Additionally, WEE1 and CDK1 were differentially expressed in PC patient tumors dichotomized by high vs low SOX2 gene expression. Importantly, pharmacological targeting of WEE1 (WEE1i) in combination with an ARSI or GR modulator re-sensitizes SOX2-positive PC cells to nuclear hormone receptor signaling inhibition in vitro, and WEE1i combined with ARSI significantly slowed tumor growth in vivo. Collectively, our data suggest SOX2 predicts NHRSI resistance, and simultaneously indicates the addition of WEE1i to improve therapeutic efficacy of NHRSIs in SOX2-positive PC.</span></p>', 'date' => '2023-05-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/37169162', 'doi' => '10.1016/j.canlet.2023.216209', 'modified' => '2023-06-15 08:58:59', 'created' => '2023-06-13 21:11:31', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 6 => array( 'id' => '4787', 'name' => 'The Effect of Metformin and Carbohydrate-Controlled Diet onDNA Methylation and Gene Expression in the Endometrium of Womenwith Polycystic Ovary Syndrome.', 'authors' => 'Garcia-Gomez E. et al.', 'description' => '<p>Polycystic ovary syndrome (PCOS) is an endocrine disease associated with infertility and metabolic disorders in reproductive-aged women. In this study, we evaluated the expression of eight genes related to endometrial function and their DNA methylation levels in the endometrium of PCOS patients and women without the disease (control group). In addition, eight of the PCOS patients underwent intervention with metformin (1500 mg/day) and a carbohydrate-controlled diet (type and quantity) for three months. Clinical and metabolic parameters were determined, and RT-qPCR and MeDIP-qPCR were used to evaluate gene expression and DNA methylation levels, respectively. Decreased expression levels of , , and genes and increased DNA methylation levels of the promoter were found in the endometrium of PCOS patients compared to controls. After metformin and nutritional intervention, some metabolic and clinical variables improved in PCOS patients. This intervention was associated with increased expression of , , and genes and reduced DNA methylation levels of the promoter in the endometrium of PCOS women. Our preliminary findings suggest that metformin and a carbohydrate-controlled diet improve endometrial function in PCOS patients, partly by modulating DNA methylation of the gene promoter and the expression of genes implicated in endometrial receptivity and insulin signaling.</p>', 'date' => '2023-04-01', 'pmid' => 'https://doi.org/10.3390%2Fijms24076857', 'doi' => '10.3390/ijms24076857', 'modified' => '2023-06-12 08:58:33', 'created' => '2023-05-05 12:34:24', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 7 => array( 'id' => '4763', 'name' => 'Chromatin profiling identifies transcriptional readthrough as a conservedmechanism for piRNA biogenesis in mosquitoes.', 'authors' => 'Qu J. et al.', 'description' => '<p>The piRNA pathway in mosquitoes differs substantially from other model organisms, with an expanded PIWI gene family and functions in antiviral defense. Here, we define core piRNA clusters as genomic loci that show ubiquitous piRNA expression in both somatic and germline tissues. These core piRNA clusters are enriched for non-retroviral endogenous viral elements (nrEVEs) in antisense orientation and depend on key biogenesis factors, Veneno, Tejas, Yb, and Shutdown. Combined transcriptome and chromatin state analyses identify transcriptional readthrough as a conserved mechanism for cluster-derived piRNA biogenesis in the vector mosquitoes Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles gambiae. Comparative analyses between the two Aedes species suggest that piRNA clusters function as traps for nrEVEs, allowing adaptation to environmental challenges such as virus infection. Our systematic transcriptome and chromatin state analyses lay the foundation for studies of gene regulation, genome evolution, and piRNA function in these important vector species.</p>', 'date' => '2023-03-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36930642', 'doi' => '10.1016/j.celrep.2023.112257', 'modified' => '2023-04-17 09:12:37', 'created' => '2023-04-14 13:41:22', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 8 => array( 'id' => '4720', 'name' => 'Activation of AKT induces EZH2-mediated β-catenin trimethylation incolorectal cancer.', 'authors' => 'Ghobashi A. H. et al.', 'description' => '<p>Colorectal cancer (CRC) develops in part through the deregulation of different signaling pathways, including activation of the WNT/β-catenin and PI3K/AKT pathways. Enhancer of zeste homolog 2 (EZH2) is a lysine methyltransferase that is involved in regulating stem cell development and differentiation and is overexpressed in CRC. However, depending on the study EZH2 has been found to be both positively and negatively correlated with the survival of CRC patients suggesting that EZH2's role in CRC may be context specific. In this study, we explored how PI3K/AKT activation alters EZH2's role in CRC. We found that activation of AKT by PTEN knockdown or by hydrogen peroxide treatment induced EZH2 phosphorylation at serine 21. Phosphorylation of EZH2 resulted in EZH2-mediated methylation of β-catenin and an associated increased interaction between β-catenin, TCF1, and RNA polymerase II. AKT activation increased β-catenin's enrichment across the genome and EZH2 inhibition reduced this enrichment by reducing the methylation of β-catenin. Furthermore, PTEN knockdown increased the expression of epithelial-mesenchymal transition (EMT)-related genes, and somewhat unexpectedly EZH2 inhibition further increased the expression of these genes. Consistent with these findings, EZH2 inhibition enhanced the migratory phenotype of PTEN knockdown cells. Overall, we demonstrated that EZH2 modulates AKT-induced changes in gene expression through the AKT/EZH2/ β-catenin axis in CRC with active PI3K/AKT signaling. Therefore, it is important to consider the use of EZH2 inhibitors in CRC with caution as these inhibitors will inhibit EZH2-mediated methylation of histone and non-histone targets such as β-catenin, which can have tumor-promoting effects.</p>', 'date' => '2023-02-01', 'pmid' => 'https://doi.org/10.1101%2F2023.01.31.526429', 'doi' => '10.1101/2023.01.31.526429', 'modified' => '2023-03-28 09:13:16', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 9 => array( 'id' => '4667', 'name' => 'Detailed molecular and epigenetic characterization of the Pig IPECJ2and Chicken SL-29 cell lines', 'authors' => 'de Vos J. et al.', 'description' => '<p>The pig IPECJ2 and chicken SL-29 cell lines are of interest because of their untransformed nature and wide use in functional studies. Molecular characterization of these cell lines is important to gain insight into possible molecular aberrations. The aims of this paper are providing a molecular and epigenetic characterization of the IPEC-J2 and SL-29 cell lines and providing a cell-line reference for the FAANG community, and future biomedical research. Whole genome sequencing , gene expression, DNA methylation , chromatin accessibility and ChIP-seq of four histone marks (H3K4me1, H3K4me3, H3K27ac, H3K27me3) and an insulator (CTCF) are used to achieve these aims. Heteroploidy (aneuploidy) of various chromosomes was observed from whole genome sequencing analysis in both cell lines. Furthermore, higher gene expression for genes located on chromosomes with aneuploidy in comparison to diploid chromosomes was observed. Regulatory complexity of gene expression, DNA methylation and chromatin accessibility was investigated through an integrative approach.</p>', 'date' => '2023-02-01', 'pmid' => 'https://doi.org/10.1016%2Fj.isci.2023.106252', 'doi' => '10.1016/j.isci.2023.106252', 'modified' => '2023-04-07 16:52:26', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 10 => array( 'id' => '4673', 'name' => 'Signal-induced enhancer activation requires Ku70 to readtopoisomerase1-DNA covalent complexes.', 'authors' => 'Tan Y. et al.', 'description' => '<p>Enhancer activation serves as the main mechanism regulating signal-dependent transcriptional programs, ensuring cellular plasticity, yet central questions persist regarding their mechanism of activation. Here, by successfully mapping topoisomerase I-DNA covalent complexes genome-wide, we find that most, if not all, acutely activated enhancers, including those induced by 17β-estradiol, dihydrotestosterone, tumor necrosis factor alpha and neuronal depolarization, are hotspots for topoisomerase I-DNA covalent complexes, functioning as epigenomic signatures read by the classic DNA damage sensor protein, Ku70. Ku70 in turn nucleates a heterochromatin protein 1 gamma (HP1γ)-mediator subunit Med26 complex to facilitate acute, but not chronic, transcriptional activation programs. Together, our data uncover a broad, unappreciated transcriptional code, required for most, if not all, acute signal-dependent enhancer activation events in both mitotic and postmitotic cells.</p>', 'date' => '2023-02-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36747093', 'doi' => '10.1038/s41594-022-00883-8', 'modified' => '2023-04-14 09:24:10', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 11 => array( 'id' => '4668', 'name' => 'Cotranscriptional demethylation induces global loss of H3K4me2 fromactive genes in Arabidopsis', 'authors' => 'Mori S. et al.', 'description' => '<p>Based on studies of animals and yeasts, methylation of histone H3 lysine 4 (H3K4me1/2/3, for mono-, di-, and tri-methylation, respectively) is regarded as the key epigenetic modification of transcriptionally active genes. In plants, however, H3K4me2 correlates negatively with transcription, and the regulatory mechanisms of this counterintuitive H3K4me2 distribution in plants remain largely unexplored. A previous genetic screen for factors regulating plant regeneration identified Arabidopsis LYSINE-SPECIFIC DEMETHYLASE 1-LIKE 3 (LDL3), which is a major H3K4me2 demethylase. Here, we show that LDL3-mediated H3K4me2 demethylation depends on the transcription elongation factor Paf1C and phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). In addition, LDL3 binds to phosphorylated RNAPII. These results suggest that LDL3 is recruited to transcribed genes by binding to elongating RNAPII and demethylates H3K4me2 cotranscriptionally. Importantly, the negative correlation between H3K4me2 and transcription is disrupted in the ldl3 mutant, demonstrating the genome-wide impacts of the transcription-driven LDL3 pathway to control H3K4me2 in plants. Our findings implicate H3K4me2 in plants as chromatin memory for transcriptionally repressive states, which ensures robust gene control.</p>', 'date' => '2023-02-01', 'pmid' => 'https://www.biorxiv.org/content/10.1101/2023.02.17.528985v1', 'doi' => '10.1101/2023.02.17.528985', 'modified' => '2023-04-14 09:31:55', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 12 => array( 'id' => '4670', 'name' => 'Epigenetic regulation of plastin 3 expression by the macrosatelliteDXZ4 and the transcriptional regulator CHD4.', 'authors' => 'Strathmann E. A. et al.', 'description' => '<p>Dysregulated Plastin 3 (PLS3) levels associate with a wide range of skeletal and neuromuscular disorders and the most common types of solid and hematopoietic cancer. Most importantly, PLS3 overexpression protects against spinal muscular atrophy. Despite its crucial role in F-actin dynamics in healthy cells and its involvement in many diseases, the mechanisms that regulate PLS3 expression are unknown. Interestingly, PLS3 is an X-linked gene and all asymptomatic SMN1-deleted individuals in SMA-discordant families who exhibit PLS3 upregulation are female, suggesting that PLS3 may escape X chromosome inactivation. To elucidate mechanisms contributing to PLS3 regulation, we performed a multi-omics analysis in two SMA-discordant families using lymphoblastoid cell lines and iPSC-derived spinal motor neurons originated from fibroblasts. We show that PLS3 tissue-specifically escapes X-inactivation. PLS3 is located ∼500 kb proximal to the DXZ4 macrosatellite, which is essential for X chromosome inactivation. By applying molecular combing in a total of 25 lymphoblastoid cell lines (asymptomatic individuals, individuals with SMA, control subjects) with variable PLS3 expression, we found a significant correlation between the copy number of DXZ4 monomers and PLS3 levels. Additionally, we identified chromodomain helicase DNA binding protein 4 (CHD4) as an epigenetic transcriptional regulator of PLS3 and validated co-regulation of the two genes by siRNA-mediated knock-down and overexpression of CHD4. We show that CHD4 binds the PLS3 promoter by performing chromatin immunoprecipitation and that CHD4/NuRD activates the transcription of PLS3 by dual-luciferase promoter assays. Thus, we provide evidence for a multilevel epigenetic regulation of PLS3 that may help to understand the protective or disease-associated PLS3 dysregulation.</p>', 'date' => '2023-02-01', 'pmid' => 'https://doi.org/10.1016%2Fj.ajhg.2023.02.004', 'doi' => '10.1016/j.ajhg.2023.02.004', 'modified' => '2023-04-14 09:36:04', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 13 => array( 'id' => '4672', 'name' => 'A dataset of definitive endoderm and hepatocyte differentiations fromhuman induced pluripotent stem cells.', 'authors' => 'Tanaka Y. et al.', 'description' => '<p>Hepatocytes are a major parenchymal cell type in the liver and play an essential role in liver function. Hepatocyte-like cells can be differentiated in vitro from induced pluripotent stem cells (iPSCs) via definitive endoderm (DE)-like cells and hepatoblast-like cells. Here, we explored the in vitro differentiation time-course of hepatocyte-like cells. We performed methylome and transcriptome analyses for hepatocyte-like cell differentiation. We also analyzed DE-like cell differentiation by methylome, transcriptome, chromatin accessibility, and GATA6 binding profiles, using finer time-course samples. In this manuscript, we provide a detailed description of the dataset and the technical validations. Our data may be valuable for the analysis of the molecular mechanisms underlying hepatocyte and DE differentiations.</p>', 'date' => '2023-02-01', 'pmid' => 'https://www.ncbi.nlm.nih.gov/pubmed/36788249', 'doi' => '10.1038/s41597-023-02001-9', 'modified' => '2023-04-14 09:41:29', 'created' => '2023-02-28 12:19:11', 'ProductsPublication' => array( [maximum depth reached] ) ), (int) 14 => array( 'id' => '4643', 'name' => 'The mineralocorticoid receptor modulates timing and location of genomicbinding by glucocorticoid receptor in response to synthetic glucocorticoidsin keratinocytes.', 'authors' => 'Carceller-Zazo E. et al.', 'description' => '<p>Glucocorticoids (GCs) exert potent antiproliferative and anti-inflammatory properties, explaining their therapeutic efficacy for skin diseases. GCs act by binding to the GC receptor (GR) and the mineralocorticoid receptor (MR), co-expressed in classical and non-classical targets including keratinocytes. Using knockout mice, we previously demonstrated that GR and MR exert essential nonoverlapping functions in skin homeostasis. These closely related receptors may homo- or heterodimerize to regulate transcription, and theoretically bind identical GC-response elements (GRE). We assessed the contribution of MR to GR genomic binding and the transcriptional response to the synthetic GC dexamethasone (Dex) using control (CO) and MR knockout (MR ) keratinocytes. GR chromatin immunoprecipitation (ChIP)-seq identified peaks common and unique to both genotypes upon Dex treatment (1 h). GREs, AP-1, TEAD, and p53 motifs were enriched in CO and MR peaks. However, GR genomic binding was 35\% reduced in MR , with significantly decreased GRE enrichm