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Picoruptor 2 sonication device




All-in-one solution
Highly reproducible
Processing of 6-16 samples
User friendly software
Five year warranty
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  •  資料
  •  関連記事
  •   お見積り
カタログ番号
フォーマット
B01080010
1 unit

環境研究、毒物学、ゲノミクスおよびエピゲノミクス、癌研究、幹細胞開発、神経科学、臨床応用、農業、その他多数を含むさまざまな研究分野でのサンプル調製において、最高のプロセスを目指して設計開発されたPicoruptorと共にDiagenodeは、2004年以来断片化に関する専門知識と経験を積み重ねてきました。

Picoruptorは、断片化おける最新のイノベーションであり、150 bpから1 kbのサンプルを断片化できるオールインワンのシアリングシステムとして最も注目されています。柔軟なサンプル量(20 µl-2 ml)とサンプルの高速並行処理(最大16サンプルまで)に欠かせないシアリングアクセサリーと消耗品もラインナップ。 冷却システムにより、高精度の温度制御が保証されます。 ユーザーフレンドリーなインターフェースはシンプルでありながら高度なモードを提供し、研究者のニーズに合わせて設計されています。ビギナーユーザーとエキスパートユーザーの両方に適切なレベル設定を提供します。

Diagenodeの断片化キット、完全検証済みチューブは最高品質だけでなく、高いコストパフォーマンスをお届けします。

アプリケーションの多様性::

  • 次世代シーケンスのためのDNA断片化
  • クロマチン断片化
  • RNA断片化
  • 組織および細胞からのタンパク質抽出(質量分析用)
  • FFPE DNA抽出
  • タンパク質凝集研究

  • 仕様書
    Ultrasonic Sonicator
  • Picoruptorのアクセサリーと消耗品はこちら

    Shearing Accessories

    Name Catalog number Throughput
    Tube holder for 0.2 ml tubes B01201144 16 samples
    Tube holder for 0.65 ml tubes B01201143 12 samples
    Tube holder for 1.5 ml tubes B01201140 6 samples
    15 ml sonication accessories B01200016 6 samples

    Shearing Consumables

    Name Catalog Number
    0.2 ml Pico Microtubes C30010020
    0.65 ml Pico Microtubes C30010011
    1.5 ml Pico Microtubes C30010016
    15 ml Pico Tubes C30010017
    15 ml Pico Tubes & sonication beads C01020031

    Find datasheet for Diagenode tubes here

  •  プロトコル集
    DNA shearing guide
    DNA shearing for Next-Generation Sequencing with the Bioruptor Pico
    Download
  • 利用可能な断片化キット

    Diagenode has optimized a range of solutions for successful chromatin preparation. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.

    For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.

    Kit choice guide

    SAMPLE TYPE SAMPLE INPUT KIT SDS
    CONCENTRATION
    NUCLEI
    ISOLATION
    CELLS
    < 100,000 Chromatin EasyShear Kit
    High SDS
    1%
    CELLS
    > 100,000 Chromatin EasyShear Kit
    Ultra Low SDS
    < 0.1%
    TISSUE
    Chromatin EasyShear Kit
    Ultra Low SDS
    < 0.1%
    PLANT TISSUE
    Chromatin EasyShear Kit
    for Plant
    0.5%
    FFPE SAMPLES
    Chromatin EasyShear Kit
    Low SDS
    0.2%
    CELLS
    Chromatin EasyShear Kit
    Low SDS
    0.2%
    TISSUE
    FFPE SAMPLES

    Guide for optimal chromatin preparation using Chromatin EasyShear Kits Read more

  •  資料
    Bioruptor Pico Quickguide MANUAL
    Download
    Bioruptor Pico Manual MANUAL
    Download
    Bioruptor ® Sonicator Brochure BROCHURE
    Diagenode’s Bioruptor® uses state-of-the-art shearing device to disrupt, disp...
    Download
    Chromatin Shearing Guide PROTOCOL
    Guide for successful chromatin preparation using the Bioruptor Pico
    Download
    DNA shearing guide PROTOCOL
    DNA shearing for Next-Generation Sequencing with the Bioruptor Pico
    Download
    Which tubes for Bioruptor® Pico DOCUMENT
    Download
    Unlock Low-Input 3D Genome Analysis with the Arima-HiC Kit APPLICATION NOTE
    By combining the optimized chemistry of the Arima-HiC kit along with new low input protocols, the...
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Picoruptor 2 sonication device (Diagenode Cat# B01080010). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    Genomic profiling of T-cell activation suggests increased sensitivity ofmemory T cells to CD28 costimulation.
    Glinos, Dafni A and Soskic, Blagoje and Williams, Cayman and Kennedy, Alanand Jostins, Luke and Sansom, David M and Trynka, Gosia
    T-cell activation is a critical driver of immune responses. The CD28 costimulation is an essential regulator of CD4 T-cell responses, however, its relative importance in naive and memory T cells is not fully understood. Using different model systems, we observe that human memory T cells are more sensitive to CD28 co...

    A genetic variant controls interferon-β gene expression in human myeloidcells by preventing C/EBP-β binding on a conserved enhancer.
    Assouvie, Anaïs and Rotival, Maxime and Hamroune, Juliette and Busso,Didier and Romeo, Paul-Henri and Quintana-Murci, Lluis and Rousselet,Germain
    Interferon β (IFN-β) is a cytokine that induces a global antiviral proteome, and regulates the adaptive immune response to infections and tumors. Its effects strongly depend on its level and timing of expression. Therefore, the transcription of its coding gene IFNB1 is strictly controlled. We have previous...

    UTX/KDM6A suppresses AP-1 and a gliogenesis program during neuraldifferentiation of human pluripotent stem cells.
    Xu, Beisi and Mulvey, Brett and Salie, Muneeb and Yang, Xiaoyang andMatsui, Yurika and Nityanandam, Anjana and Fan, Yiping and Peng, Jamy C
    BACKGROUND: UTX/KDM6A is known to interact and influence multiple different chromatin modifiers to promote an open chromatin environment to facilitate gene activation, but its molecular activities in developmental gene regulation remain unclear. RESULTS: We report that in human neural stem cells, UTX binding correla...

    Combined treatment with CBP and BET inhibitors reverses inadvertentactivation of detrimental super enhancer programs in DIPG cells.
    Wiese, M and Hamdan, FH and Kubiak, K and Diederichs, C and Gielen, GHand Nussbaumer, G and Carcaboso, AM and Hulleman, E and Johnsen, SA andKramm, CM
    Diffuse intrinsic pontine gliomas (DIPG) are the most aggressive brain tumors in children with 5-year survival rates of only 2%. About 85% of all DIPG are characterized by a lysine-to-methionine substitution in histone 3, which leads to global H3K27 hypomethylation accompanied by H3K27 hyperacetylation. Hyperacetyla...

    Methylation in pericytes after acute injury promotes chronic kidneydisease.
    Chou, YH and Pan, SY and Shao, YH and Shih, HM and Wei, SY andLai, CF and Chiang, WC and Schrimpf, C and Yang, KC and Lai, LC andChen, YM and Chu, TS and Lin, SL
    The origin and fate of renal myofibroblasts is not clear after acute kidney injury (AKI). Here, we demonstrate that myofibroblasts were activated from quiescent pericytes (qPericytes) and the cell numbers increased after ischemia/reperfusion injury-induced AKI (IRI-AKI). Myofibroblasts underwent apoptosis during ren...

    Exploring the virulence gene interactome with CRISPR/dCas9 in the humanmalaria parasite.
    Bryant, JM and Baumgarten, S and Dingli, F and Loew, D and Sinha, A andClaës, A and Preiser, PR and Dedon, PC and Scherf, A
    Mutually exclusive expression of the var multigene family is key to immune evasion and pathogenesis in Plasmodium falciparum, but few factors have been shown to play a direct role. We adapted a CRISPR-based proteomics approach to identify novel factors associated with var genes in their natural chromatin context. Ca...

    Targeted bisulfite sequencing for biomarker discovery.
    Morselli, M and Farrell, C and Rubbi, L and Fehling, HL and Henkhaus, Rand Pellegrini, M
    Cytosine methylation is one of the best studied epigenetic modifications. In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development and differentiation and its dysregulation can lead to or is associated with dis...

    Battle of the sex chromosomes: competition between X- and Y-chromosomeencoded proteins for partner interaction and chromatin occupancy drivesmulti-copy gene expression and evolution in muroid rodents.
    Moretti, C and Blanco, M and Ialy-Radio, C and Serrentino, ME and Gobé,C and Friedman, R and Battail, C and Leduc, M and Ward, MA and Vaiman, Dand Tores, F and Cocquet, J
    Transmission distorters (TDs) are genetic elements that favor their own transmission to the detriments of others. Slx/Slxl1 (Sycp3-like-X-linked and Slx-like1) and Sly (Sycp3-like-Y-linked) are TDs which have been co-amplified on the X and Y chromosomes of Mus species. They are involved in an intragenomic conflict i...

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