Diagenode

Picoruptor 2 sonication device




All-in-one solution
Highly reproducible
Processing of 6-16 samples
User friendly software
Five year warranty
  •  プロトコル集
  •  資料
  •  関連記事
  •   お見積り
カタログ番号
フォーマット
B01080010
1 unit

環境研究、毒物学、ゲノミクスおよびエピゲノミクス、癌研究、幹細胞開発、神経科学、臨床応用、農業、その他多数を含むさまざまな研究分野でのサンプル調製において、最高のプロセスを目指して設計開発されたPicoruptorと共にDiagenodeは、2004年以来断片化に関する専門知識と経験を積み重ねてきました。

Picoruptorは、断片化おける最新のイノベーションであり、150 bpから1 kbのサンプルを断片化できるオールインワンのシアリングシステムとして最も注目されています。柔軟なサンプル量(20 µl-2 ml)とサンプルの高速並行処理(最大16サンプルまで)に欠かせないシアリングアクセサリーと消耗品もラインナップ。 冷却システムにより、高精度の温度制御が保証されます。 ユーザーフレンドリーなインターフェースはシンプルでありながら高度なモードを提供し、研究者のニーズに合わせて設計されています。ビギナーユーザーとエキスパートユーザーの両方に適切なレベル設定を提供します。

Diagenodeの断片化キット、完全検証済みチューブは最高品質だけでなく、高いコストパフォーマンスをお届けします。

アプリケーションの多様性::

  • 次世代シーケンスのためのDNA断片化
  • クロマチン断片化
  • RNA断片化
  • 組織および細胞からのタンパク質抽出(質量分析用)
  • FFPE DNA抽出
  • タンパク質凝集研究

  • 仕様書
    Ultrasonic Sonicator
  • Picoruptorのアクセサリーと消耗品はこちら

    Shearing Accessories

    Name Catalog number Throughput
    Tube holder for 0.2 ml tubes B01201144 16 samples
    Tube holder for 0.65 ml tubes B01201143 12 samples
    Tube holder for 1.5 ml tubes B01201140 6 samples
    15 ml sonication accessories B01200016 6 samples

    Shearing Consumables

    Name Catalog Number
    0.2 ml Pico Microtubes C30010020
    0.65 ml Pico Microtubes C30010011
    1.5 ml Pico Microtubes C30010016
    15 ml Pico Tubes C30010017
    15 ml Pico Tubes & sonication beads C01020031

    Find datasheet for Diagenode tubes here

  •  プロトコル集
    DNA shearing guide
    DNA shearing for Next-Generation Sequencing with the Bioruptor Pico
    Download
  • 利用可能な断片化キット

    Diagenode has optimized a range of solutions for successful chromatin preparation. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.

    For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.

    Kit choice guide

    SAMPLE TYPE SAMPLE INPUT KIT SDS
    CONCENTRATION
    NUCLEI
    ISOLATION
    CELLS
    < 100,000 Chromatin EasyShear Kit
    High SDS
    1%
    CELLS
    > 100,000 Chromatin EasyShear Kit
    Ultra Low SDS
    < 0.1%
    TISSUE
    Chromatin EasyShear Kit
    Ultra Low SDS
    < 0.1%
    PLANT TISSUE
    Chromatin EasyShear Kit
    for Plant
    0.5%
    FFPE SAMPLES
    Chromatin EasyShear Kit
    Low SDS
    0.2%
    CELLS
    Chromatin EasyShear Kit
    Low SDS
    0.2%
    TISSUE
    FFPE SAMPLES

    Guide for optimal chromatin preparation using Chromatin EasyShear Kits Read more

  •  資料
    Bioruptor Pico Quickguide MANUAL
    Download
    Bioruptor Pico Manual MANUAL
    Download
    Bioruptor ® Sonicator Brochure BROCHURE
    Diagenode’s Bioruptor® uses state-of-the-art shearing device to disrupt, disp...
    Download
    Chromatin Shearing Guide PROTOCOL
    Guide for successful chromatin preparation using the Bioruptor Pico
    Download
    DNA shearing guide PROTOCOL
    DNA shearing for Next-Generation Sequencing with the Bioruptor Pico
    Download
    Which tubes for Bioruptor® Pico DOCUMENT
    Download
    Unlock Low-Input 3D Genome Analysis with the Arima-HiC Kit APPLICATION NOTE
    By combining the optimized chemistry of the Arima-HiC kit along with new low input protocols, the...
    Download
  •  出版物

    How to properly cite this product in your work

    Diagenode strongly recommends using this: Picoruptor 2 sonication device (Diagenode Cat# B01080010). Click here to copy to clipboard.

    Using our products in your publication? Let us know!

    The lncRNA and the transcription factor WRKY42 target common cell wallEXTENSIN encoding genes to trigger root hair cell elongation.
    Pacheco, J. M. et al.
    Plant long noncoding RNAs (lncRNAs) are key chromatin dynamics regulators, directing the transcriptional programs driving a wide variety of developmental outputs. Recently, we uncovered how the lncRNA () directly recognizes the locus encoding the root hair (RH) master regulator () modulating its transcriptional acti...

    Placental uptake and metabolism of 25(OH)Vitamin D determines itsactivity within the fetoplacental unit
    Ashley, B. et al.
    Pregnancy 25-hydroxyvitamin D (25(OH)D) concentrations are associated with maternal and fetal health outcomes, but the underlying mechanisms have not been elucidated. Using physiological human placental perfusion approaches and intact villous explants we demonstrate a role for the placenta in regulating the relation...

    Waves of sumoylation support transcription dynamics during adipocytedifferentiation
    Zhao, X. et al.
    Tight control of gene expression networks required for adipose tissue formation and plasticity is essential for adaptation to energy needs and environmental cues. However, little is known about the mechanisms that orchestrate the dramatic transcriptional changes leading to adipocyte differentiation. We investigated ...

    Androgen receptor positively regulates gonadotropin-releasing hormonereceptor in pituitary gonadotropes.
    Ryan, Genevieve E. et al.
    Within pituitary gonadotropes, the gonadotropin-releasing hormone receptor (GnRHR) receives hypothalamic input from GnRH neurons that is critical for reproduction. Previous studies have suggested that androgens may regulate GnRHR, although the mechanisms remain unknown. In this study, we demonstrated that androgens ...

    Genetic perturbation of PU.1 binding and chromatin looping at neutrophilenhancers associates with autoimmune disease.
    Watt, Stephen et al.
    Neutrophils play fundamental roles in innate immune response, shape adaptive immunity, and are a potentially causal cell type underpinning genetic associations with immune system traits and diseases. Here, we profile the binding of myeloid master regulator PU.1 in primary neutrophils across nearly a hundred voluntee...

    Fra-1 regulates its target genes via binding to remote enhancers withoutexerting major control on chromatin architecture in triple negative breastcancers.
    Bejjani, Fabienne and Tolza, Claire and Boulanger, Mathias and Downes,Damien and Romero, Raphaël and Maqbool, Muhammad Ahmad and Zine ElAabidine, Amal and Andrau, Jean-Christophe and Lebre, Sophie and Brehelin,Laurent and Parrinello, Hughes and Rohmer,
    The ubiquitous family of dimeric transcription factors AP-1 is made up of Fos and Jun family proteins. It has long been thought to operate principally at gene promoters and how it controls transcription is still ill-understood. The Fos family protein Fra-1 is overexpressed in triple negative breast cancers (TNBCs) w...

    Cell-specific alterations inPitx1regulatory landscape activation caused bythe loss of a single enhancer
    Rouco, R. et al.
    Most developmental genes rely on multiple transcriptional enhancers for their accurate expression during embryogenesis. Because enhancers may have partially redundant activities, the loss of one of them often leads to a partial loss of gene expression and concurrent moderate phenotypic outcome, if any. While such a ...

    Transgenic mice for in vivo epigenome editing with CRISPR-based systems
    Gemberling, M. et al.
    The discovery, characterization, and adaptation of the RNA-guided clustered regularly interspersed short palindromic repeat (CRISPR)-Cas9 system has greatly increased the ease with which genome and epigenome editing can be performed. Fusion of chromatin-modifying domains to the nuclease-deactivated form of Cas9 (dCa...

    Transcriptional programming drives Ibrutinib-resistance evolution in mantlecell lymphoma.
    Zhao, Xiaohong et al.
    Ibrutinib, a bruton's tyrosine kinase (BTK) inhibitor, provokes robust clinical responses in aggressive mantle cell lymphoma (MCL), yet many patients relapse with lethal Ibrutinib-resistant (IR) disease. Here, using genomic, chemical proteomic, and drug screen profiling, we report that enhancer remodeling-mediated t...

    Coordinated changes in gene expression, H1 variant distribution and genome3D conformation in response to H1 depletion
    Serna-Pujol, Nuria and Salinas-Pena, Monica and Mugianesi, Francesca and LeDily, François and Marti-Renom, Marc A. and Jordan, Albert
    Up to seven members of the histone H1 family may contribute to chromatin compaction and its regulation in human somatic cells. In breast cancer cells, knock-down of multiple H1 variants deregulates many genes, promotes the appearance of genome-wide accessibility sites and triggers an interferon response via activati...

    REPROGRAMMING CBX8-PRC1 FUNCTION WITH A POSITIVE ALLOSTERICMODULATOR
    Suh, J. L. et al.
    Canonical targeting of Polycomb Repressive Complex 1 (PRC1) to repress developmental genes is mediated by cell type-specific, paralogous chromobox (CBX) proteins (CBX2, 4, 6, 7 and 8). Based on their central role in silencing and their misregulation associated with human disease including cancer, CBX proteins are at...

    Germline activity of the heat shock factor HSF-1 programs theinsulin-receptor daf-2 in C. elegans
    Das, S. et al.
    The mechanisms by which maternal stress alters offspring phenotypes remain poorly understood. Here we report that the heat shock transcription factor HSF-1, activated in the C. elegans maternal germline upon stress, epigenetically programs the insulin-like receptor daf-2 by increasing repressive H3K9me2 levels throu...

    The epigenetic landscape in purified myonuclei from fast and slow muscles
    Bengtsen, M. et al.
    Muscle cells have different phenotypes adapted to different usage and can be grossly divided into fast/glycolytic and slow/oxidative types. While most muscles contain a mixture of such fiber types, we aimed at providing a genome-wide analysis of chromatin environment by ChIP-Seq in two muscle extremes, the almost co...

    The glucocorticoid receptor recruits the COMPASS complex to regulateinflammatory transcription at macrophage enhancers.
    Greulich, Franziska et al.
    Glucocorticoids (GCs) are effective anti-inflammatory drugs; yet, their mechanisms of action are poorly understood. GCs bind to the glucocorticoid receptor (GR), a ligand-gated transcription factor controlling gene expression in numerous cell types. Here, we characterize GR's protein interactome and find the SETD1A ...

    A distinct metabolic response characterizes sensitivity to EZH2inhibition in multiple myeloma.
    Nylund P. et al.
    Multiple myeloma (MM) is a heterogeneous haematological disease that remains clinically challenging. Increased activity of the epigenetic silencer EZH2 is a common feature in patients with poor prognosis. Previous findings have demonstrated that metabolic profiles can be sensitive markers for response to treatment i...

    BAF complexes drive proliferation and block myogenic differentiation in fusion-positive rhabdomyosarcoma
    Laubscher et. al.
    Rhabdomyosarcoma (RMS) is a pediatric malignancy of skeletal muscle lineage. The aggressive alveolar subtype is characterized by t(2;13) or t(1;13) translocations encoding for PAX3- or PAX7-FOXO1 chimeric transcription factors, respectively, and are referred to as fusion positive RMS (FP-RMS). The fusion gene alters...

    A Tumor Suppressor Enhancer of PTEN in T-cell development and leukemia
    L. Tottone at al.
    Long-range oncogenic enhancers play an important role in cancer. Yet, whether similar regulation of tumor suppressor genes is relevant remains unclear. Loss of expression of PTEN is associated with the pathogenesis of various cancers, including T-cell leukemia (T-ALL). Here, we identify a highly conserved distal enh...

    Stronger induction of trained immunity by mucosal BCG or MTBVAC vaccination compared to standard intradermal vaccination.
    Vierboom, M.P.M. et al.
    BCG vaccination can strengthen protection against pathogens through the induction of epigenetic and metabolic reprogramming of innate immune cells, a process called trained immunity. We and others recently demonstrated that mucosal or intravenous BCG better protects rhesus macaques from infection and TB disease than...

    Postoperative abdominal sepsis induces selective and persistent changes inCTCF binding within the MHC-II region of human monocytes.
    Siegler B. et al.
    BACKGROUND: Postoperative abdominal infections belong to the most common triggers of sepsis and septic shock in intensive care units worldwide. While monocytes play a central role in mediating the initial host response to infections, sepsis-induced immune dysregulation is characterized by a defective antigen present...

    S-adenosyl-l-homocysteine hydrolase links methionine metabolism to thecircadian clock and chromatin remodeling.
    Greco C. M. et al.
    Circadian gene expression driven by transcription activators CLOCK and BMAL1 is intimately associated with dynamic chromatin remodeling. However, how cellular metabolism directs circadian chromatin remodeling is virtually unexplored. We report that the S-adenosylhomocysteine (SAH) hydrolyzing enzyme adenosylhomocyst...

    Genomic profiling of T-cell activation suggests increased sensitivity ofmemory T cells to CD28 costimulation.
    Glinos, Dafni A and Soskic, Blagoje and Williams, Cayman and Kennedy, Alanand Jostins, Luke and Sansom, David M and Trynka, Gosia
    T-cell activation is a critical driver of immune responses. The CD28 costimulation is an essential regulator of CD4 T-cell responses, however, its relative importance in naive and memory T cells is not fully understood. Using different model systems, we observe that human memory T cells are more sensitive to CD28 co...

    A genetic variant controls interferon-β gene expression in human myeloidcells by preventing C/EBP-β binding on a conserved enhancer.
    Assouvie, Anaïs and Rotival, Maxime and Hamroune, Juliette and Busso,Didier and Romeo, Paul-Henri and Quintana-Murci, Lluis and Rousselet,Germain
    Interferon β (IFN-β) is a cytokine that induces a global antiviral proteome, and regulates the adaptive immune response to infections and tumors. Its effects strongly depend on its level and timing of expression. Therefore, the transcription of its coding gene IFNB1 is strictly controlled. We have previous...

    BCG Vaccination Induces Long-Term Functional Reprogramming of HumanNeutrophils.
    Moorlag, Simone J C F M and Rodriguez-Rosales, Yessica Alina and Gillard,Joshua and Fanucchi, Stephanie and Theunissen, Kate and Novakovic, Borisand de Bont, Cynthia M and Negishi, Yutaka and Fok, Ezio T and Kalafati,Lydia and Verginis, Panayotis and M
    The tuberculosis vaccine bacillus Calmette-Guérin (BCG) protects against some heterologous infections, probably via induction of non-specific innate immune memory in monocytes and natural killer (NK) cells, a process known as trained immunity. Recent studies have revealed that the induction of trained immunit...

    Macrophage Immune Memory Controls Endometriosis in Mice and Humans.
    Jeljeli, Mohamed and Riccio, Luiza G C and Chouzenoux, Sandrine and Moresi,Fabiana and Toullec, Laurie and Doridot, Ludivine and Nicco, Carole andBourdon, Mathilde and Marcellin, Louis and Santulli, Pietro and Abrão,Mauricio S and Chapron, Charles and
    Endometriosis is a frequent, chronic, inflammatory gynecological disease characterized by the presence of ectopic endometrial tissue causing pain and infertility. Macrophages have a central role in lesion establishment and maintenance by driving chronic inflammation and tissue remodeling. Macrophages can be reprogra...

    UTX/KDM6A suppresses AP-1 and a gliogenesis program during neuraldifferentiation of human pluripotent stem cells.
    Xu, Beisi and Mulvey, Brett and Salie, Muneeb and Yang, Xiaoyang andMatsui, Yurika and Nityanandam, Anjana and Fan, Yiping and Peng, Jamy C
    BACKGROUND: UTX/KDM6A is known to interact and influence multiple different chromatin modifiers to promote an open chromatin environment to facilitate gene activation, but its molecular activities in developmental gene regulation remain unclear. RESULTS: We report that in human neural stem cells, UTX binding correla...

    Epigenetic regulation of the lineage specificity of primary human dermallymphatic and blood vascular endothelial cells.
    Tacconi, Carlotta and He, Yuliang and Ducoli, Luca and Detmar, Michael
    Lymphatic and blood vascular endothelial cells (ECs) share several molecular and developmental features. However, these two cell types possess distinct phenotypic signatures, reflecting their different biological functions. Despite significant advances in elucidating how the specification of lymphatic and blood vasc...

    Combined treatment with CBP and BET inhibitors reverses inadvertentactivation of detrimental super enhancer programs in DIPG cells.
    Wiese, M and Hamdan, FH and Kubiak, K and Diederichs, C and Gielen, GHand Nussbaumer, G and Carcaboso, AM and Hulleman, E and Johnsen, SA andKramm, CM
    Diffuse intrinsic pontine gliomas (DIPG) are the most aggressive brain tumors in children with 5-year survival rates of only 2%. About 85% of all DIPG are characterized by a lysine-to-methionine substitution in histone 3, which leads to global H3K27 hypomethylation accompanied by H3K27 hyperacetylation. Hyperacetyla...

    Methylation in pericytes after acute injury promotes chronic kidneydisease.
    Chou, YH and Pan, SY and Shao, YH and Shih, HM and Wei, SY andLai, CF and Chiang, WC and Schrimpf, C and Yang, KC and Lai, LC andChen, YM and Chu, TS and Lin, SL
    The origin and fate of renal myofibroblasts is not clear after acute kidney injury (AKI). Here, we demonstrate that myofibroblasts were activated from quiescent pericytes (qPericytes) and the cell numbers increased after ischemia/reperfusion injury-induced AKI (IRI-AKI). Myofibroblasts underwent apoptosis during ren...

    Exploring the virulence gene interactome with CRISPR/dCas9 in the humanmalaria parasite.
    Bryant, JM and Baumgarten, S and Dingli, F and Loew, D and Sinha, A andClaës, A and Preiser, PR and Dedon, PC and Scherf, A
    Mutually exclusive expression of the var multigene family is key to immune evasion and pathogenesis in Plasmodium falciparum, but few factors have been shown to play a direct role. We adapted a CRISPR-based proteomics approach to identify novel factors associated with var genes in their natural chromatin context. Ca...

    Targeted bisulfite sequencing for biomarker discovery.
    Morselli, M and Farrell, C and Rubbi, L and Fehling, HL and Henkhaus, Rand Pellegrini, M
    Cytosine methylation is one of the best studied epigenetic modifications. In mammals, DNA methylation patterns vary among cells and is mainly found in the CpG context. DNA methylation is involved in important processes during development and differentiation and its dysregulation can lead to or is associated with dis...

    Battle of the sex chromosomes: competition between X- and Y-chromosomeencoded proteins for partner interaction and chromatin occupancy drivesmulti-copy gene expression and evolution in muroid rodents.
    Moretti, C and Blanco, M and Ialy-Radio, C and Serrentino, ME and Gobé,C and Friedman, R and Battail, C and Leduc, M and Ward, MA and Vaiman, Dand Tores, F and Cocquet, J
    Transmission distorters (TDs) are genetic elements that favor their own transmission to the detriments of others. Slx/Slxl1 (Sycp3-like-X-linked and Slx-like1) and Sly (Sycp3-like-Y-linked) are TDs which have been co-amplified on the X and Y chromosomes of Mus species. They are involved in an intragenomic conflict i...

  •  関連商品

ニュース

 すべてのニュースを見る


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics