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<div class="row">
<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus',
'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p>
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<p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p>
<p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p>
<p><strong>Application versatility</strong>:</p>
<ul>
<li>DNA shearing for Next-Generation-Sequencing</li>
<li>Chromatin shearing</li>
<li>RNA shearing</li>
<li>Protein extraction from tissues and cells (also for mass spectrometry)</li>
<li>FFPE DNA extraction</li>
<li>Protein aggregation studies</li>
<li>CUT&RUN - shearing of input DNA for NGS</li>
</ul>
<div style="background-color: #f1f3f4; margin: 10px; padding: 50px;">
<p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p>
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<div class="extra-spaced"><img alt="Bioruptor Sonication for DNA shearing for Next-Generation-Sequencing" src="https://www.diagenode.com/img/product/shearing_technologies/guarantie.png" /></div>
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<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
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<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
</tbody>
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<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
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<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td>
</tr>
</tbody>
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<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
'label3' => 'Available shearing Kits',
'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p>
<p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p>
<h2>Kit choice guide</h2>
<table style="border: 0;" valign="center">
<tbody>
<tr style="background: #fff;">
<th class="text-center"></th>
<th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th>
<th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th>
<th class="text-center" style="font-size: 17px;">KIT</th>
<th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th>
<th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th>
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<tr style="background: #fff;">
<td colspan="7"></td>
</tr>
<tr style="background: #fff;">
<td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td>
<td class="text-center" style="border-bottom: 1px solid #dedede;">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td>
<td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px;">> 100,000</td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">PLANT TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td>
<td class="text-center" style="font-size: 17px;">0.5%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">0.2%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td colspan="7"></td>
</tr>
<tr style="background: #fff;">
<td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td>
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center"></td>
<td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td>
<td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
</td>
<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
</tr>
</tbody>
</table>
<div class="extra-spaced">
<h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3>
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'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
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<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
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<span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Phone number</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">City</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-1784">
<option value="">-- select a country --</option>
<option value="AF">Afghanistan</option>
<option value="AX">Åland Islands</option>
<option value="AL">Albania</option>
<option value="DZ">Algeria</option>
<option value="AS">American Samoa</option>
<option value="AD">Andorra</option>
<option value="AO">Angola</option>
<option value="AI">Anguilla</option>
<option value="AQ">Antarctica</option>
<option value="AG">Antigua and Barbuda</option>
<option value="AR">Argentina</option>
<option value="AM">Armenia</option>
<option value="AW">Aruba</option>
<option value="AU">Australia</option>
<option value="AT">Austria</option>
<option value="AZ">Azerbaijan</option>
<option value="BS">Bahamas</option>
<option value="BH">Bahrain</option>
<option value="BD">Bangladesh</option>
<option value="BB">Barbados</option>
<option value="BY">Belarus</option>
<option value="BE">Belgium</option>
<option value="BZ">Belize</option>
<option value="BJ">Benin</option>
<option value="BM">Bermuda</option>
<option value="BT">Bhutan</option>
<option value="BO">Bolivia</option>
<option value="BQ">Bonaire, Sint Eustatius and Saba</option>
<option value="BA">Bosnia and Herzegovina</option>
<option value="BW">Botswana</option>
<option value="BV">Bouvet Island</option>
<option value="BR">Brazil</option>
<option value="IO">British Indian Ocean Territory</option>
<option value="BN">Brunei Darussalam</option>
<option value="BG">Bulgaria</option>
<option value="BF">Burkina Faso</option>
<option value="BI">Burundi</option>
<option value="KH">Cambodia</option>
<option value="CM">Cameroon</option>
<option value="CA">Canada</option>
<option value="CV">Cape Verde</option>
<option value="KY">Cayman Islands</option>
<option value="CF">Central African Republic</option>
<option value="TD">Chad</option>
<option value="CL">Chile</option>
<option value="CN">China</option>
<option value="CX">Christmas Island</option>
<option value="CC">Cocos (Keeling) Islands</option>
<option value="CO">Colombia</option>
<option value="KM">Comoros</option>
<option value="CG">Congo</option>
<option value="CD">Congo, The Democratic Republic of the</option>
<option value="CK">Cook Islands</option>
<option value="CR">Costa Rica</option>
<option value="CI">Côte d'Ivoire</option>
<option value="HR">Croatia</option>
<option value="CU">Cuba</option>
<option value="CW">Curaçao</option>
<option value="CY">Cyprus</option>
<option value="CZ">Czech Republic</option>
<option value="DK">Denmark</option>
<option value="DJ">Djibouti</option>
<option value="DM">Dominica</option>
<option value="DO">Dominican Republic</option>
<option value="EC">Ecuador</option>
<option value="EG">Egypt</option>
<option value="SV">El Salvador</option>
<option value="GQ">Equatorial Guinea</option>
<option value="ER">Eritrea</option>
<option value="EE">Estonia</option>
<option value="ET">Ethiopia</option>
<option value="FK">Falkland Islands (Malvinas)</option>
<option value="FO">Faroe Islands</option>
<option value="FJ">Fiji</option>
<option value="FI">Finland</option>
<option value="FR">France</option>
<option value="GF">French Guiana</option>
<option value="PF">French Polynesia</option>
<option value="TF">French Southern Territories</option>
<option value="GA">Gabon</option>
<option value="GM">Gambia</option>
<option value="GE">Georgia</option>
<option value="DE">Germany</option>
<option value="GH">Ghana</option>
<option value="GI">Gibraltar</option>
<option value="GR">Greece</option>
<option value="GL">Greenland</option>
<option value="GD">Grenada</option>
<option value="GP">Guadeloupe</option>
<option value="GU">Guam</option>
<option value="GT">Guatemala</option>
<option value="GG">Guernsey</option>
<option value="GN">Guinea</option>
<option value="GW">Guinea-Bissau</option>
<option value="GY">Guyana</option>
<option value="HT">Haiti</option>
<option value="HM">Heard Island and McDonald Islands</option>
<option value="VA">Holy See (Vatican City State)</option>
<option value="HN">Honduras</option>
<option value="HK">Hong Kong</option>
<option value="HU">Hungary</option>
<option value="IS">Iceland</option>
<option value="IN">India</option>
<option value="ID">Indonesia</option>
<option value="IR">Iran, Islamic Republic of</option>
<option value="IQ">Iraq</option>
<option value="IE">Ireland</option>
<option value="IM">Isle of Man</option>
<option value="IL">Israel</option>
<option value="IT">Italy</option>
<option value="JM">Jamaica</option>
<option value="JP">Japan</option>
<option value="JE">Jersey</option>
<option value="JO">Jordan</option>
<option value="KZ">Kazakhstan</option>
<option value="KE">Kenya</option>
<option value="KI">Kiribati</option>
<option value="KP">Korea, Democratic People's Republic of</option>
<option value="KR">Korea, Republic of</option>
<option value="KW">Kuwait</option>
<option value="KG">Kyrgyzstan</option>
<option value="LA">Lao People's Democratic Republic</option>
<option value="LV">Latvia</option>
<option value="LB">Lebanon</option>
<option value="LS">Lesotho</option>
<option value="LR">Liberia</option>
<option value="LY">Libya</option>
<option value="LI">Liechtenstein</option>
<option value="LT">Lithuania</option>
<option value="LU">Luxembourg</option>
<option value="MO">Macao</option>
<option value="MK">Macedonia, Republic of</option>
<option value="MG">Madagascar</option>
<option value="MW">Malawi</option>
<option value="MY">Malaysia</option>
<option value="MV">Maldives</option>
<option value="ML">Mali</option>
<option value="MT">Malta</option>
<option value="MH">Marshall Islands</option>
<option value="MQ">Martinique</option>
<option value="MR">Mauritania</option>
<option value="MU">Mauritius</option>
<option value="YT">Mayotte</option>
<option value="MX">Mexico</option>
<option value="FM">Micronesia, Federated States of</option>
<option value="MD">Moldova</option>
<option value="MC">Monaco</option>
<option value="MN">Mongolia</option>
<option value="ME">Montenegro</option>
<option value="MS">Montserrat</option>
<option value="MA">Morocco</option>
<option value="MZ">Mozambique</option>
<option value="MM">Myanmar</option>
<option value="NA">Namibia</option>
<option value="NR">Nauru</option>
<option value="NP">Nepal</option>
<option value="NL">Netherlands</option>
<option value="NC">New Caledonia</option>
<option value="NZ">New Zealand</option>
<option value="NI">Nicaragua</option>
<option value="NE">Niger</option>
<option value="NG">Nigeria</option>
<option value="NU">Niue</option>
<option value="NF">Norfolk Island</option>
<option value="MP">Northern Mariana Islands</option>
<option value="NO">Norway</option>
<option value="OM">Oman</option>
<option value="PK">Pakistan</option>
<option value="PW">Palau</option>
<option value="PS">Palestine, State of</option>
<option value="PA">Panama</option>
<option value="PG">Papua New Guinea</option>
<option value="PY">Paraguay</option>
<option value="PE">Peru</option>
<option value="PH">Philippines</option>
<option value="PN">Pitcairn</option>
<option value="PL">Poland</option>
<option value="PT">Portugal</option>
<option value="PR">Puerto Rico</option>
<option value="QA">Qatar</option>
<option value="RE">Réunion</option>
<option value="RO">Romania</option>
<option value="RU">Russian Federation</option>
<option value="RW">Rwanda</option>
<option value="BL">Saint Barthélemy</option>
<option value="SH">Saint Helena, Ascension and Tristan da Cunha</option>
<option value="KN">Saint Kitts and Nevis</option>
<option value="LC">Saint Lucia</option>
<option value="MF">Saint Martin (French part)</option>
<option value="PM">Saint Pierre and Miquelon</option>
<option value="VC">Saint Vincent and the Grenadines</option>
<option value="WS">Samoa</option>
<option value="SM">San Marino</option>
<option value="ST">Sao Tome and Principe</option>
<option value="SA">Saudi Arabia</option>
<option value="SN">Senegal</option>
<option value="RS">Serbia</option>
<option value="SC">Seychelles</option>
<option value="SL">Sierra Leone</option>
<option value="SG">Singapore</option>
<option value="SX">Sint Maarten (Dutch part)</option>
<option value="SK">Slovakia</option>
<option value="SI">Slovenia</option>
<option value="SB">Solomon Islands</option>
<option value="SO">Somalia</option>
<option value="ZA">South Africa</option>
<option value="GS">South Georgia and the South Sandwich Islands</option>
<option value="ES">Spain</option>
<option value="LK">Sri Lanka</option>
<option value="SD">Sudan</option>
<option value="SR">Suriname</option>
<option value="SS">South Sudan</option>
<option value="SJ">Svalbard and Jan Mayen</option>
<option value="SZ">Swaziland</option>
<option value="SE">Sweden</option>
<option value="CH">Switzerland</option>
<option value="SY">Syrian Arab Republic</option>
<option value="TW">Taiwan</option>
<option value="TJ">Tajikistan</option>
<option value="TZ">Tanzania</option>
<option value="TH">Thailand</option>
<option value="TL">Timor-Leste</option>
<option value="TG">Togo</option>
<option value="TK">Tokelau</option>
<option value="TO">Tonga</option>
<option value="TT">Trinidad and Tobago</option>
<option value="TN">Tunisia</option>
<option value="TR">Turkey</option>
<option value="TM">Turkmenistan</option>
<option value="TC">Turks and Caicos Islands</option>
<option value="TV">Tuvalu</option>
<option value="UG">Uganda</option>
<option value="UA">Ukraine</option>
<option value="AE">United Arab Emirates</option>
<option value="GB">United Kingdom</option>
<option value="US" selected="selected">United States</option>
<option value="UM">United States Minor Outlying Islands</option>
<option value="UY">Uruguay</option>
<option value="UZ">Uzbekistan</option>
<option value="VU">Vanuatu</option>
<option value="VE">Venezuela</option>
<option value="VN">Viet Nam</option>
<option value="VG">Virgin Islands, British</option>
<option value="VI">Virgin Islands, U.S.</option>
<option value="WF">Wallis and Futuna</option>
<option value="EH">Western Sahara</option>
<option value="YE">Yemen</option>
<option value="ZM">Zambia</option>
<option value="ZW">Zimbabwe</option>
</select><script>
$('#country_selector_quote-1784').selectize();
</script><br />
</div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">State</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][state]" id="state-1784" maxlength="3" type="text"/><br />
</div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/> </div>
</div>
<div class="row collapse" id="email_v">
<div class="small-3 large-2 columns">
<span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Comment</span>
</div>
<div class="small-9 large-10 columns">
<textarea name="data[Quote][comment]" placeholder="Additional comments" cols="30" rows="6" id="QuoteComment"></textarea> </div>
</div>
<!------------SERVICES PARTICULAR FORM START---------------->
<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->
<div class="row collapse">
<div class="small-3 large-2 columns">
</div>
<div class="small-9 large-10 columns">
<div class="recaptcha"><div id="recaptcha662261cea3660"></div></div> </div>
</div>
<br />
<div class="row collapse">
<div class="small-3 large-2 columns">
</div>
<div class="small-9 large-10 columns">
<button id="submit_btn-1784" class="alert button expand" form="Quote-1784" type="submit">Contact me</button> </div>
</div>
</form><script>
var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c';
function postToPardot(formAction, id) {
$('#pardot-form-handler').load(function(){
$('#Quote-' + id).attr('action', formAction);
$('#Quote-' + id).submit();
});
$('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize());
}
$(document).ready(function() {
$('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>');
$('#Quote-1784').attr('action','javascript:postToPardot(\'' + $('#Quote-1784').attr('action') + '\', 1784)');
});
$("#Quote-1784 #submit_btn-1784").click(function (e) {
if($(this).closest('form')[0].checkValidity()){
e.preventDefault();
//disable the submit button
$("#Quote-1784 #submit_btn-1784").attr("disabled", true);
$("#Quote-1784 #submit_btn-1784").html("Processing...");
//submit the form
$("#Quote-1784").submit();
}
})
</script>
<script>
if ($("#Quote-1784 #country_selector_quote-1784.selectized").val() == 'US') {
var val = $("#state-1784").val();
$("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
if (val.length == 2) {
$("#state-1784").val(val);
}
$("#state-1784").parent().parent().show();
} else if ($("#Quote-1784 #country_selector_quote-1784.selectized").val() == 'CA') {
var val = $("#state-1784").val();
$("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
if (val.length == 2) {
$("#state-1784").val(val);
}
$("#state-1784").parent().parent().show();
} else if ($("#Quote-1784 #country_selector_quote-1784.selectized").val() == 'DE') {
var val = $("#state-1784").val();
$("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
if (val.length == 2) {
$("#state-1784").val(val);
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$("#state-1784").parent().parent().show();
} else {
$("#Quote-1784 #state-1784").parent().parent().hide();
$("#Quote-1784 #state-1784").replaceWith('<input name="data[Quote][state]" maxlength="255" type="text" id="state-1784" value="">');
}
$("#Quote-1784 #country_selector_quote-1784.selectized").change(function() {
if (this.value == 'US') {
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} else if (this.value == 'DE') {
$("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="BW">Baden-Württemberg (BW)</option><option value="BY">Bayern (BY)</option><option value="BE">Berlin (BE)</option><option value="BB">Brandeburg (BB)</option><option value="HB">Bremen (HB)</option><option value="HH">Hamburg (HH)</option><option value="HE">Hessen (HE)</option><option value="MV">Mecklenburg-Vorpommern (MV)</option><option value="NI">Niedersachsen (NI)</option><option value="NW">Nordrhein-Westfalen (NW)</option><option value="RP">Rheinland-Pfalz (RP)</option><option value="SL">Saarland (SL)</option><option value="SN">Sachsen (SN)</option><option value ="SA">Sachsen-Anhalt (SA)</option><option value="SH">Schleswig-Holstein (SH)</option><option value="TH">Thüringen</option></select>');
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});
</script>
<a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptor-plus-sonication-device" data-reveal-id="quoteModal-1784" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Plus sonication device</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/protein-extraction-kit-50-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C20000020</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2617" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2617" id="CartAdd/2617Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2617" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Protein Extraction kit for Bioruptor<sup>®</sup> Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
'C20000020',
'435',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
'C20000020',
'435',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="protein-extraction-kit-50-rxns" data-reveal-id="cartModal-2617" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Protein Extraction kit for Bioruptor® Plus</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/15-ml-tpx-tubes-50-pc"><img src="/img/product/shearing_technologies/C30010009_tube.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C30010009</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2621" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2621" id="CartAdd/2621Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2621" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor<sup>®</sup> Plus TPX tubes</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'C30010009',
'120',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'C30010009',
'120',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-tpx-tubes-50-pc" data-reveal-id="cartModal-2621" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Plus TPX tubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/15-ml-bioruptor-tubes-50-pc"><img src="/img/product/shearing_technologies/C30010017_tube.png" alt="15 ml tubes" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C30010017</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2631" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2631" id="CartAdd/2631Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2631" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor® Pico Tubes</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor® Pico Tubes',
'C30010017',
'115',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor® Pico Tubes',
'C30010017',
'115',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-bioruptor-tubes-50-pc" data-reveal-id="cartModal-2631" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Pico Tubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns"><img src="/img/product/shearing_technologies/C01020031_tube.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C01020031</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1875" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1875" id="CartAdd/1875Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1875" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor<sup>®</sup> Pico Tubes & sonication beads</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Pico Tubes & sonication beads',
'C01020031',
'225',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Pico Tubes & sonication beads',
'C01020031',
'225',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-bioruptor-tubes-sonication-beads-50-rxns" data-reveal-id="cartModal-1875" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Pico Tubes & sonication beads</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/bioruptorpico2"><img src="/img/product/shearing_technologies/B01080000-1.jpg" alt="Bioruptor Pico" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01080010</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: QUOTE MODAL --><div id="quoteModal-3046" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Bioruptor<sup>®</sup> Pico sonication device</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>
</div>
</div>
<form action="/en/quotes/quote?id=3046" id="Quote-3046" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="3046" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="MExEWnYraStBcHRxZXNQbTVmZ0luQT09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="bioruptorpico2" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/>
<div class="row collapse">
<h2>Contact Information</h2>
<div class="small-3 large-2 columns">
<span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Phone number</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">City</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-3046">
<option value="">-- select a country --</option>
<option value="AF">Afghanistan</option>
<option value="AX">Åland Islands</option>
<option value="AL">Albania</option>
<option value="DZ">Algeria</option>
<option value="AS">American Samoa</option>
<option value="AD">Andorra</option>
<option value="AO">Angola</option>
<option value="AI">Anguilla</option>
<option value="AQ">Antarctica</option>
<option value="AG">Antigua and Barbuda</option>
<option value="AR">Argentina</option>
<option value="AM">Armenia</option>
<option value="AW">Aruba</option>
<option value="AU">Australia</option>
<option value="AT">Austria</option>
<option value="AZ">Azerbaijan</option>
<option value="BS">Bahamas</option>
<option value="BH">Bahrain</option>
<option value="BD">Bangladesh</option>
<option value="BB">Barbados</option>
<option value="BY">Belarus</option>
<option value="BE">Belgium</option>
<option value="BZ">Belize</option>
<option value="BJ">Benin</option>
<option value="BM">Bermuda</option>
<option value="BT">Bhutan</option>
<option value="BO">Bolivia</option>
<option value="BQ">Bonaire, Sint Eustatius and Saba</option>
<option value="BA">Bosnia and Herzegovina</option>
<option value="BW">Botswana</option>
<option value="BV">Bouvet Island</option>
<option value="BR">Brazil</option>
<option value="IO">British Indian Ocean Territory</option>
<option value="BN">Brunei Darussalam</option>
<option value="BG">Bulgaria</option>
<option value="BF">Burkina Faso</option>
<option value="BI">Burundi</option>
<option value="KH">Cambodia</option>
<option value="CM">Cameroon</option>
<option value="CA">Canada</option>
<option value="CV">Cape Verde</option>
<option value="KY">Cayman Islands</option>
<option value="CF">Central African Republic</option>
<option value="TD">Chad</option>
<option value="CL">Chile</option>
<option value="CN">China</option>
<option value="CX">Christmas Island</option>
<option value="CC">Cocos (Keeling) Islands</option>
<option value="CO">Colombia</option>
<option value="KM">Comoros</option>
<option value="CG">Congo</option>
<option value="CD">Congo, The Democratic Republic of the</option>
<option value="CK">Cook Islands</option>
<option value="CR">Costa Rica</option>
<option value="CI">Côte d'Ivoire</option>
<option value="HR">Croatia</option>
<option value="CU">Cuba</option>
<option value="CW">Curaçao</option>
<option value="CY">Cyprus</option>
<option value="CZ">Czech Republic</option>
<option value="DK">Denmark</option>
<option value="DJ">Djibouti</option>
<option value="DM">Dominica</option>
<option value="DO">Dominican Republic</option>
<option value="EC">Ecuador</option>
<option value="EG">Egypt</option>
<option value="SV">El Salvador</option>
<option value="GQ">Equatorial Guinea</option>
<option value="ER">Eritrea</option>
<option value="EE">Estonia</option>
<option value="ET">Ethiopia</option>
<option value="FK">Falkland Islands (Malvinas)</option>
<option value="FO">Faroe Islands</option>
<option value="FJ">Fiji</option>
<option value="FI">Finland</option>
<option value="FR">France</option>
<option value="GF">French Guiana</option>
<option value="PF">French Polynesia</option>
<option value="TF">French Southern Territories</option>
<option value="GA">Gabon</option>
<option value="GM">Gambia</option>
<option value="GE">Georgia</option>
<option value="DE">Germany</option>
<option value="GH">Ghana</option>
<option value="GI">Gibraltar</option>
<option value="GR">Greece</option>
<option value="GL">Greenland</option>
<option value="GD">Grenada</option>
<option value="GP">Guadeloupe</option>
<option value="GU">Guam</option>
<option value="GT">Guatemala</option>
<option value="GG">Guernsey</option>
<option value="GN">Guinea</option>
<option value="GW">Guinea-Bissau</option>
<option value="GY">Guyana</option>
<option value="HT">Haiti</option>
<option value="HM">Heard Island and McDonald Islands</option>
<option value="VA">Holy See (Vatican City State)</option>
<option value="HN">Honduras</option>
<option value="HK">Hong Kong</option>
<option value="HU">Hungary</option>
<option value="IS">Iceland</option>
<option value="IN">India</option>
<option value="ID">Indonesia</option>
<option value="IR">Iran, Islamic Republic of</option>
<option value="IQ">Iraq</option>
<option value="IE">Ireland</option>
<option value="IM">Isle of Man</option>
<option value="IL">Israel</option>
<option value="IT">Italy</option>
<option value="JM">Jamaica</option>
<option value="JP">Japan</option>
<option value="JE">Jersey</option>
<option value="JO">Jordan</option>
<option value="KZ">Kazakhstan</option>
<option value="KE">Kenya</option>
<option value="KI">Kiribati</option>
<option value="KP">Korea, Democratic People's Republic of</option>
<option value="KR">Korea, Republic of</option>
<option value="KW">Kuwait</option>
<option value="KG">Kyrgyzstan</option>
<option value="LA">Lao People's Democratic Republic</option>
<option value="LV">Latvia</option>
<option value="LB">Lebanon</option>
<option value="LS">Lesotho</option>
<option value="LR">Liberia</option>
<option value="LY">Libya</option>
<option value="LI">Liechtenstein</option>
<option value="LT">Lithuania</option>
<option value="LU">Luxembourg</option>
<option value="MO">Macao</option>
<option value="MK">Macedonia, Republic of</option>
<option value="MG">Madagascar</option>
<option value="MW">Malawi</option>
<option value="MY">Malaysia</option>
<option value="MV">Maldives</option>
<option value="ML">Mali</option>
<option value="MT">Malta</option>
<option value="MH">Marshall Islands</option>
<option value="MQ">Martinique</option>
<option value="MR">Mauritania</option>
<option value="MU">Mauritius</option>
<option value="YT">Mayotte</option>
<option value="MX">Mexico</option>
<option value="FM">Micronesia, Federated States of</option>
<option value="MD">Moldova</option>
<option value="MC">Monaco</option>
<option value="MN">Mongolia</option>
<option value="ME">Montenegro</option>
<option value="MS">Montserrat</option>
<option value="MA">Morocco</option>
<option value="MZ">Mozambique</option>
<option value="MM">Myanmar</option>
<option value="NA">Namibia</option>
<option value="NR">Nauru</option>
<option value="NP">Nepal</option>
<option value="NL">Netherlands</option>
<option value="NC">New Caledonia</option>
<option value="NZ">New Zealand</option>
<option value="NI">Nicaragua</option>
<option value="NE">Niger</option>
<option value="NG">Nigeria</option>
<option value="NU">Niue</option>
<option value="NF">Norfolk Island</option>
<option value="MP">Northern Mariana Islands</option>
<option value="NO">Norway</option>
<option value="OM">Oman</option>
<option value="PK">Pakistan</option>
<option value="PW">Palau</option>
<option value="PS">Palestine, State of</option>
<option value="PA">Panama</option>
<option value="PG">Papua New Guinea</option>
<option value="PY">Paraguay</option>
<option value="PE">Peru</option>
<option value="PH">Philippines</option>
<option value="PN">Pitcairn</option>
<option value="PL">Poland</option>
<option value="PT">Portugal</option>
<option value="PR">Puerto Rico</option>
<option value="QA">Qatar</option>
<option value="RE">Réunion</option>
<option value="RO">Romania</option>
<option value="RU">Russian Federation</option>
<option value="RW">Rwanda</option>
<option value="BL">Saint Barthélemy</option>
<option value="SH">Saint Helena, Ascension and Tristan da Cunha</option>
<option value="KN">Saint Kitts and Nevis</option>
<option value="LC">Saint Lucia</option>
<option value="MF">Saint Martin (French part)</option>
<option value="PM">Saint Pierre and Miquelon</option>
<option value="VC">Saint Vincent and the Grenadines</option>
<option value="WS">Samoa</option>
<option value="SM">San Marino</option>
<option value="ST">Sao Tome and Principe</option>
<option value="SA">Saudi Arabia</option>
<option value="SN">Senegal</option>
<option value="RS">Serbia</option>
<option value="SC">Seychelles</option>
<option value="SL">Sierra Leone</option>
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<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Pico sonication device</h6>
</div>
</div>
</li>
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'id' => '3046',
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'name' => 'Bioruptor<sup>®</sup> Pico sonication device',
'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p>
<p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p>
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<p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p>
<p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p>
<p><strong>Application versatility</strong>:</p>
<ul>
<li>DNA shearing for Next-Generation-Sequencing</li>
<li>Chromatin shearing</li>
<li>RNA shearing</li>
<li>Protein extraction from tissues and cells (also for mass spectrometry)</li>
<li>FFPE DNA extraction</li>
<li>Protein aggregation studies</li>
<li>CUT&RUN - shearing of input DNA for NGS</li>
</ul>
<div style="background-color: #f1f3f4; margin: 10px; padding: 50px;">
<p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p>
</div>
<div class="extra-spaced"><img alt="Bioruptor Sonication for DNA shearing for Next-Generation-Sequencing" src="https://www.diagenode.com/img/product/shearing_technologies/guarantie.png" /></div>
<div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div>
<div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div>
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'label1' => 'Specifications',
'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center>
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'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico',
'info2' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
'label3' => 'Available shearing Kits',
'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p>
<p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p>
<h2>Kit choice guide</h2>
<table style="border: 0;" valign="center">
<tbody>
<tr style="background: #fff;">
<th class="text-center"></th>
<th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th>
<th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th>
<th class="text-center" style="font-size: 17px;">KIT</th>
<th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th>
<th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th>
</tr>
<tr style="background: #fff;">
<td colspan="7"></td>
</tr>
<tr style="background: #fff;">
<td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td>
<td class="text-center" style="border-bottom: 1px solid #dedede;">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td>
<td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px;">> 100,000</td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">PLANT TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td>
<td class="text-center" style="font-size: 17px;">0.5%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">0.2%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td colspan="7"></td>
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<td colspan="6"></td>
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<td colspan="6"></td>
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<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center"></td>
<td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td>
<td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
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<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
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<td class="text-center"></td>
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<div class="extra-spaced">
<h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3>
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<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus',
'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
'format' => '1 unit',
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'price_USD' => '29500',
'price_GBP' => '19500',
'price_JPY' => '3524625',
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'price_AUD' => '73750',
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'meta_title' => 'Bioruptor® Plus sonication device',
'meta_keywords' => '',
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(int) 1 => array(
'id' => '2617',
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'name' => 'Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
'description' => '<p><span>Protein extraction from tissues is the first step for many biochemical and analytical techniques (PAGE, Western blotting, mass spectrometry, etc.) or protein purification. Diagenode’s </span>Bioruptor<sup>®</sup> Plus<span> used in combination with the Protein Extraction kit ensures efficient disruption, homogenization and high yields of proteins from tissues. Tubes are already pre-filled with the protein extraction beads which have been designed to enhance the protein extraction process.</span></p>',
'label1' => '',
'info1' => '',
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'format' => '50 rxns',
'catalog_number' => 'C20000020',
'old_catalog_number' => 'AL-002-0050',
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'search_order' => '01-Accessory',
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'price_USD' => '435',
'price_GBP' => '290',
'price_JPY' => '50910',
'price_CNY' => '',
'price_AUD' => '1088',
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'online' => true,
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'meta_title' => 'Protein Extraction kit',
'meta_keywords' => '',
'meta_description' => 'Protein Extraction kit',
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'ProductsRelated' => array(
[maximum depth reached]
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[maximum depth reached]
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),
(int) 2 => array(
'id' => '2621',
'antibody_id' => null,
'name' => '15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'description' => '<p><span>TPX® Polymethylpentene (PMP) tubes improve sonication and shearing efficiency. TPX® is a hard plastic which ameliorates the transmission of the ultrasound to the sample. TPX® tubes have been validated for use on the Bioruptors® Standard and Bioruptors® Plus. Recommended sample volume: 500 μl - 2 ml.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 pc',
'catalog_number' => 'C30010009',
'old_catalog_number' => 'M-UN-15',
'sf_code' => 'C30010009-',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '100',
'price_USD' => '120',
'price_GBP' => '95',
'price_JPY' => '15665',
'price_CNY' => '',
'price_AUD' => '300',
'country' => 'ALL',
'except_countries' => 'Japan',
'quote' => false,
'in_stock' => false,
'featured' => false,
'no_promo' => false,
'online' => true,
'master' => true,
'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-tpx-tubes-50-pc',
'meta_title' => '15 ml TPX tubes',
'meta_keywords' => '',
'meta_description' => '15 ml TPX tubes',
'modified' => '2017-08-19 18:39:10',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
[maximum depth reached]
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[maximum depth reached]
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),
(int) 3 => array(
'id' => '2631',
'antibody_id' => null,
'name' => '15 ml Bioruptor® Pico Tubes',
'description' => '<p><span>The 15 ml Bioruptor® Tubes strongly improve protein extraction efficiency. The tubes have been thoroughly validated for use on the Bioruptor® Pico and are strongly recommended for extraction of proteins using this instrument. Recommended sample volume: 500 μl - 2 ml.</span></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label1' => '',
'info1' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label2' => '',
'info2' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'label3' => '',
'info3' => '<p></p>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>
<script src="chrome-extension://hhojmcideegachlhfgfdhailpfhgknjm/web_accessible_resources/index.js"></script>',
'format' => '50 pc',
'catalog_number' => 'C30010017',
'old_catalog_number' => '',
'sf_code' => 'C30010017-1860',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '75',
'price_USD' => '115',
'price_GBP' => '65',
'price_JPY' => '11750',
'price_CNY' => '',
'price_AUD' => '288',
'country' => 'ALL',
'except_countries' => 'None',
'quote' => false,
'in_stock' => false,
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'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-bioruptor-tubes-50-pc',
'meta_title' => '15 ml Bioruptor® Tubes',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes',
'modified' => '2022-01-25 04:02:13',
'created' => '2015-06-29 14:08:20',
'ProductsRelated' => array(
[maximum depth reached]
),
'Image' => array(
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(int) 4 => array(
'id' => '1875',
'antibody_id' => null,
'name' => '15 ml Bioruptor<sup>®</sup> Pico Tubes & sonication beads',
'description' => '<p><span>The 15 ml Bioruptor® Tubes & sonication beads (diameter > 1 mm) for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Bioruptor® Pico and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 500 μl - 2 ml.</span></p>',
'label1' => '',
'info1' => '',
'label2' => '',
'info2' => '',
'label3' => '',
'info3' => '',
'format' => '50 rxns',
'catalog_number' => 'C01020031',
'old_catalog_number' => '',
'sf_code' => 'C01020031-6000',
'type' => 'ACC',
'search_order' => '01-Accessory',
'price_EUR' => '160',
'price_USD' => '225',
'price_GBP' => '145',
'price_JPY' => '25065',
'price_CNY' => '',
'price_AUD' => '562',
'country' => 'ALL',
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'in_stock' => false,
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'last_datasheet_update' => '0000-00-00',
'slug' => '15-ml-bioruptor-tubes-sonication-beads-50-rxns',
'meta_title' => '15 ml Bioruptor® Tubes & sonication beads',
'meta_keywords' => '',
'meta_description' => '15 ml Bioruptor® Tubes & sonication beads',
'modified' => '2021-12-20 12:27:43',
'created' => '2015-06-29 14:08:20',
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(int) 5 => array(
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'name' => 'Bioruptor<sup>®</sup> Pico sonication device',
'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p>
<p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p>
</div>
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<p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p>
<p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p>
<p><strong>Application versatility</strong>:</p>
<ul>
<li>DNA shearing for Next-Generation-Sequencing</li>
<li>Chromatin shearing</li>
<li>RNA shearing</li>
<li>Protein extraction from tissues and cells (also for mass spectrometry)</li>
<li>FFPE DNA extraction</li>
<li>Protein aggregation studies</li>
<li>CUT&RUN - shearing of input DNA for NGS</li>
</ul>
<div style="background-color: #f1f3f4; margin: 10px; padding: 50px;">
<p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p>
</div>
<div class="extra-spaced"><img alt="Bioruptor Sonication for DNA shearing for Next-Generation-Sequencing" src="https://www.diagenode.com/img/product/shearing_technologies/guarantie.png" /></div>
<div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div>
<div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div>
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<div id="ConnectiveDocSignExtentionInstalled" data-extension-version="1.0.4"></div>',
'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico',
'info2' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
'label3' => 'Available shearing Kits',
'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p>
<p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p>
<h2>Kit choice guide</h2>
<table style="border: 0;" valign="center">
<tbody>
<tr style="background: #fff;">
<th class="text-center"></th>
<th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th>
<th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th>
<th class="text-center" style="font-size: 17px;">KIT</th>
<th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th>
<th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th>
</tr>
<tr style="background: #fff;">
<td colspan="7"></td>
</tr>
<tr style="background: #fff;">
<td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td>
<td class="text-center" style="border-bottom: 1px solid #dedede;">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td>
<td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px;">> 100,000</td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">PLANT TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td>
<td class="text-center" style="font-size: 17px;">0.5%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">0.2%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td colspan="7"></td>
</tr>
<tr style="background: #fff;">
<td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td>
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center"></td>
<td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td>
<td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
</td>
<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
</tr>
</tbody>
</table>
<div class="extra-spaced">
<h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3>
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'meta_title' => 'Bioruptor® Pico sonication device',
'meta_keywords' => '',
'meta_description' => 'Bioruptor® Pico sonication device',
'modified' => '2023-06-20 18:16:03',
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'id' => '8',
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'name' => 'Chromatin shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
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<div class="large-12 columns">Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption and homogenization of tissues and cultured cells are required. <strong>Diagenode's Bioruptor</strong><sup>® </sup><strong>Plus</strong> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) combined with unique devices such as beads or tubes acting as sonication « enhancers » or dedicated reagents (such as our RNA extraction reagent) to efficiently disrupt tissues and cultured cells in just one step to deliver high quality RNA extraction.</div>
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<p><span>Diagenode’s Bioruptor</span><sup><span>® </span></sup><span>Standard uses a gentle method of sonication to retain the integrity of DNA and/or biological complexes, including chromatin, protein-protein binding, protein-DNA complexes and other biochemical and biological assay systems. </span></p>
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</div>
<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Plus sonication device</h6>
</div>
</div>
</li>
<li>
<div class="row">
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<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
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<h6 style="height:60px">Protein Extraction kit for Bioruptor® Plus</h6>
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</li>
<li>
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<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
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<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
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</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-tpx-tubes-50-pc" data-reveal-id="cartModal-2621" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Plus TPX tubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
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<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor® Pico Tubes</strong> to my shopping cart.</p>
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<div class="small-6 medium-6 large-6 columns">
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</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-bioruptor-tubes-50-pc" data-reveal-id="cartModal-2631" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Pico Tubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns"><img src="/img/product/shearing_technologies/C01020031_tube.png" alt="some alt" class="th"/></a> </div>
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<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor<sup>®</sup> Pico Tubes & sonication beads</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Pico Tubes & sonication beads',
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</div>
</div>
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</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Pico Tubes & sonication beads</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/bioruptorpico2"><img src="/img/product/shearing_technologies/B01080000-1.jpg" alt="Bioruptor Pico" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01080010</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
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<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Bioruptor<sup>®</sup> Pico sonication device</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>
</div>
</div>
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<h2>Contact Information</h2>
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<span class="prefix">Phone number</span>
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<option value="BW">Botswana</option>
<option value="BV">Bouvet Island</option>
<option value="BR">Brazil</option>
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<option value="CV">Cape Verde</option>
<option value="KY">Cayman Islands</option>
<option value="CF">Central African Republic</option>
<option value="TD">Chad</option>
<option value="CL">Chile</option>
<option value="CN">China</option>
<option value="CX">Christmas Island</option>
<option value="CC">Cocos (Keeling) Islands</option>
<option value="CO">Colombia</option>
<option value="KM">Comoros</option>
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<option value="CI">Côte d'Ivoire</option>
<option value="HR">Croatia</option>
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<option value="CY">Cyprus</option>
<option value="CZ">Czech Republic</option>
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<option value="DJ">Djibouti</option>
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<option value="GM">Gambia</option>
<option value="GE">Georgia</option>
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<option value="GD">Grenada</option>
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<option value="GN">Guinea</option>
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<option value="GY">Guyana</option>
<option value="HT">Haiti</option>
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<option value="HU">Hungary</option>
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<option value="IQ">Iraq</option>
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<option value="MZ">Mozambique</option>
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<option value="NI">Nicaragua</option>
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<option value="NO">Norway</option>
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<option value="PW">Palau</option>
<option value="PS">Palestine, State of</option>
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<option value="PG">Papua New Guinea</option>
<option value="PY">Paraguay</option>
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<option value="PN">Pitcairn</option>
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<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Pico sonication device</h6>
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</li>
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<p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p>
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<p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p>
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<p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p>
<p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p>
<p><strong>Application versatility</strong>:</p>
<ul>
<li>DNA shearing for Next-Generation-Sequencing</li>
<li>Chromatin shearing</li>
<li>RNA shearing</li>
<li>Protein extraction from tissues and cells (also for mass spectrometry)</li>
<li>FFPE DNA extraction</li>
<li>Protein aggregation studies</li>
<li>CUT&RUN - shearing of input DNA for NGS</li>
</ul>
<div style="background-color: #f1f3f4; margin: 10px; padding: 50px;">
<p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p>
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<div class="extra-spaced"><img alt="Bioruptor Sonication for DNA shearing for Next-Generation-Sequencing" src="https://www.diagenode.com/img/product/shearing_technologies/guarantie.png" /></div>
<div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div>
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'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico',
'info2' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
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<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
</tbody>
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<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td>
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<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
'label3' => 'Available shearing Kits',
'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p>
<p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p>
<h2>Kit choice guide</h2>
<table style="border: 0;" valign="center">
<tbody>
<tr style="background: #fff;">
<th class="text-center"></th>
<th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th>
<th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th>
<th class="text-center" style="font-size: 17px;">KIT</th>
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<td colspan="7"></td>
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<td class="text-center" style="border-bottom: 1px solid #dedede;">
<div class="label alert" style="font-size: 17px;">CELLS</div>
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<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td>
<td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px;">> 100,000</td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">PLANT TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td>
<td class="text-center" style="font-size: 17px;">0.5%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">0.2%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td colspan="7"></td>
</tr>
<tr style="background: #fff;">
<td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td>
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center"></td>
<td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td>
<td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
</td>
<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
</tr>
</tbody>
</table>
<div class="extra-spaced">
<h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3>
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<div class="row">
<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
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<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
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'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
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'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
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<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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'name' => '15 ml Bioruptor® Pico Tubes',
'description' => '<p><span>The 15 ml Bioruptor® Tubes strongly improve protein extraction efficiency. The tubes have been thoroughly validated for use on the Bioruptor® Pico and are strongly recommended for extraction of proteins using this instrument. Recommended sample volume: 500 μl - 2 ml.</span></p>
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'name' => '15 ml Bioruptor<sup>®</sup> Pico Tubes & sonication beads',
'description' => '<p><span>The 15 ml Bioruptor® Tubes & sonication beads (diameter > 1 mm) for chromatin shearing strongly improve chromatin sonication efficiency. The tubes and beads have been thoroughly validated for use on the Bioruptor® Pico and are strongly recommended for chromatin shearing applications using this instrument. Recommended sample volume: 500 μl - 2 ml.</span></p>',
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'format' => '50 rxns',
'catalog_number' => 'C01020031',
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'sf_code' => 'C01020031-6000',
'type' => 'ACC',
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'price_JPY' => '25065',
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'name' => 'Bioruptor<sup>®</sup> Pico sonication device',
'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p>
<p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p>
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<p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p>
<p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p>
<p><strong>Application versatility</strong>:</p>
<ul>
<li>DNA shearing for Next-Generation-Sequencing</li>
<li>Chromatin shearing</li>
<li>RNA shearing</li>
<li>Protein extraction from tissues and cells (also for mass spectrometry)</li>
<li>FFPE DNA extraction</li>
<li>Protein aggregation studies</li>
<li>CUT&RUN - shearing of input DNA for NGS</li>
</ul>
<div style="background-color: #f1f3f4; margin: 10px; padding: 50px;">
<p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p>
</div>
<div class="extra-spaced"><img alt="Bioruptor Sonication for DNA shearing for Next-Generation-Sequencing" src="https://www.diagenode.com/img/product/shearing_technologies/guarantie.png" /></div>
<div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div>
<div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div>
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'info1' => '<center><img alt="Ultrasonic Sonicator" src="https://www.diagenode.com/img/product/shearing_technologies/pico-table.jpg" /></center>
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'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico',
'info2' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
'label3' => 'Available shearing Kits',
'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p>
<p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p>
<h2>Kit choice guide</h2>
<table style="border: 0;" valign="center">
<tbody>
<tr style="background: #fff;">
<th class="text-center"></th>
<th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th>
<th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th>
<th class="text-center" style="font-size: 17px;">KIT</th>
<th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th>
<th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th>
</tr>
<tr style="background: #fff;">
<td colspan="7"></td>
</tr>
<tr style="background: #fff;">
<td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td>
<td class="text-center" style="border-bottom: 1px solid #dedede;">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td>
<td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px;">> 100,000</td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">PLANT TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td>
<td class="text-center" style="font-size: 17px;">0.5%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">0.2%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td colspan="7"></td>
</tr>
<tr style="background: #fff;">
<td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td>
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center"></td>
<td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td>
<td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
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<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
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<h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3>
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<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
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<div class="page" title="Page 7">
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<tbody>
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<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
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<p><strong>SDS concentration</strong></p>
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<p>< 0.1%</p>
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<td style="text-align: center;">
<p>0.2%</p>
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<td style="text-align: center;">
<p>1%</p>
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<td style="text-align: center;">
<p>0.5%</p>
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<p><strong>Nuclei isolation</strong></p>
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<td style="text-align: center;">
<p>Yes</p>
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<td style="text-align: center;">
<p>Yes</p>
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<td style="text-align: center;">
<p>No</p>
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<td style="text-align: center;">
<p>Yes</p>
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<p><strong>Allows for shearing of... cells/tissue</strong></p>
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<p>100 million cells</p>
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<td style="text-align: center;">
<p>100 million cells</p>
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<td style="text-align: center;">
<p>100 million cells</p>
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<p>up to 25 g of tissue</p>
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<p><strong>Corresponding to shearing buffers from</strong></p>
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<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
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<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
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<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
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<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
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(int) 5 => 'NO',
(int) 6 => 'SE',
(int) 7 => 'FI',
(int) 8 => 'NL',
(int) 9 => 'BE',
(int) 10 => 'LU',
(int) 11 => 'FR',
(int) 12 => 'DE',
(int) 13 => 'CH',
(int) 14 => 'AT',
(int) 15 => 'ES',
(int) 16 => 'IT',
(int) 17 => 'PT'
)
$outsource = false
$other_formats = array()
$edit = ''
$testimonials = ''
$featured_testimonials = ''
$related_products = '<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/bioruptor-plus-sonication-device"><img src="/img/product/shearing_technologies/bioruptor_plus.jpg" alt="Bioruptor Plus Sonication Device" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01020001</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: QUOTE MODAL --><div id="quoteModal-1784" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Bioruptor<sup>®</sup> Plus sonication device</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>
</div>
</div>
<form action="/en/quotes/quote?id=1784" id="Quote-1784" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="1784" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="SWZSSHJaRlNqc1FESUVjMEJKcWw5QT09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="bioruptor-plus-sonication-device" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/>
<div class="row collapse">
<h2>Contact Information</h2>
<div class="small-3 large-2 columns">
<span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Phone number</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">City</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-1784">
<option value="">-- select a country --</option>
<option value="AF">Afghanistan</option>
<option value="AX">Åland Islands</option>
<option value="AL">Albania</option>
<option value="DZ">Algeria</option>
<option value="AS">American Samoa</option>
<option value="AD">Andorra</option>
<option value="AO">Angola</option>
<option value="AI">Anguilla</option>
<option value="AQ">Antarctica</option>
<option value="AG">Antigua and Barbuda</option>
<option value="AR">Argentina</option>
<option value="AM">Armenia</option>
<option value="AW">Aruba</option>
<option value="AU">Australia</option>
<option value="AT">Austria</option>
<option value="AZ">Azerbaijan</option>
<option value="BS">Bahamas</option>
<option value="BH">Bahrain</option>
<option value="BD">Bangladesh</option>
<option value="BB">Barbados</option>
<option value="BY">Belarus</option>
<option value="BE">Belgium</option>
<option value="BZ">Belize</option>
<option value="BJ">Benin</option>
<option value="BM">Bermuda</option>
<option value="BT">Bhutan</option>
<option value="BO">Bolivia</option>
<option value="BQ">Bonaire, Sint Eustatius and Saba</option>
<option value="BA">Bosnia and Herzegovina</option>
<option value="BW">Botswana</option>
<option value="BV">Bouvet Island</option>
<option value="BR">Brazil</option>
<option value="IO">British Indian Ocean Territory</option>
<option value="BN">Brunei Darussalam</option>
<option value="BG">Bulgaria</option>
<option value="BF">Burkina Faso</option>
<option value="BI">Burundi</option>
<option value="KH">Cambodia</option>
<option value="CM">Cameroon</option>
<option value="CA">Canada</option>
<option value="CV">Cape Verde</option>
<option value="KY">Cayman Islands</option>
<option value="CF">Central African Republic</option>
<option value="TD">Chad</option>
<option value="CL">Chile</option>
<option value="CN">China</option>
<option value="CX">Christmas Island</option>
<option value="CC">Cocos (Keeling) Islands</option>
<option value="CO">Colombia</option>
<option value="KM">Comoros</option>
<option value="CG">Congo</option>
<option value="CD">Congo, The Democratic Republic of the</option>
<option value="CK">Cook Islands</option>
<option value="CR">Costa Rica</option>
<option value="CI">Côte d'Ivoire</option>
<option value="HR">Croatia</option>
<option value="CU">Cuba</option>
<option value="CW">Curaçao</option>
<option value="CY">Cyprus</option>
<option value="CZ">Czech Republic</option>
<option value="DK">Denmark</option>
<option value="DJ">Djibouti</option>
<option value="DM">Dominica</option>
<option value="DO">Dominican Republic</option>
<option value="EC">Ecuador</option>
<option value="EG">Egypt</option>
<option value="SV">El Salvador</option>
<option value="GQ">Equatorial Guinea</option>
<option value="ER">Eritrea</option>
<option value="EE">Estonia</option>
<option value="ET">Ethiopia</option>
<option value="FK">Falkland Islands (Malvinas)</option>
<option value="FO">Faroe Islands</option>
<option value="FJ">Fiji</option>
<option value="FI">Finland</option>
<option value="FR">France</option>
<option value="GF">French Guiana</option>
<option value="PF">French Polynesia</option>
<option value="TF">French Southern Territories</option>
<option value="GA">Gabon</option>
<option value="GM">Gambia</option>
<option value="GE">Georgia</option>
<option value="DE">Germany</option>
<option value="GH">Ghana</option>
<option value="GI">Gibraltar</option>
<option value="GR">Greece</option>
<option value="GL">Greenland</option>
<option value="GD">Grenada</option>
<option value="GP">Guadeloupe</option>
<option value="GU">Guam</option>
<option value="GT">Guatemala</option>
<option value="GG">Guernsey</option>
<option value="GN">Guinea</option>
<option value="GW">Guinea-Bissau</option>
<option value="GY">Guyana</option>
<option value="HT">Haiti</option>
<option value="HM">Heard Island and McDonald Islands</option>
<option value="VA">Holy See (Vatican City State)</option>
<option value="HN">Honduras</option>
<option value="HK">Hong Kong</option>
<option value="HU">Hungary</option>
<option value="IS">Iceland</option>
<option value="IN">India</option>
<option value="ID">Indonesia</option>
<option value="IR">Iran, Islamic Republic of</option>
<option value="IQ">Iraq</option>
<option value="IE">Ireland</option>
<option value="IM">Isle of Man</option>
<option value="IL">Israel</option>
<option value="IT">Italy</option>
<option value="JM">Jamaica</option>
<option value="JP">Japan</option>
<option value="JE">Jersey</option>
<option value="JO">Jordan</option>
<option value="KZ">Kazakhstan</option>
<option value="KE">Kenya</option>
<option value="KI">Kiribati</option>
<option value="KP">Korea, Democratic People's Republic of</option>
<option value="KR">Korea, Republic of</option>
<option value="KW">Kuwait</option>
<option value="KG">Kyrgyzstan</option>
<option value="LA">Lao People's Democratic Republic</option>
<option value="LV">Latvia</option>
<option value="LB">Lebanon</option>
<option value="LS">Lesotho</option>
<option value="LR">Liberia</option>
<option value="LY">Libya</option>
<option value="LI">Liechtenstein</option>
<option value="LT">Lithuania</option>
<option value="LU">Luxembourg</option>
<option value="MO">Macao</option>
<option value="MK">Macedonia, Republic of</option>
<option value="MG">Madagascar</option>
<option value="MW">Malawi</option>
<option value="MY">Malaysia</option>
<option value="MV">Maldives</option>
<option value="ML">Mali</option>
<option value="MT">Malta</option>
<option value="MH">Marshall Islands</option>
<option value="MQ">Martinique</option>
<option value="MR">Mauritania</option>
<option value="MU">Mauritius</option>
<option value="YT">Mayotte</option>
<option value="MX">Mexico</option>
<option value="FM">Micronesia, Federated States of</option>
<option value="MD">Moldova</option>
<option value="MC">Monaco</option>
<option value="MN">Mongolia</option>
<option value="ME">Montenegro</option>
<option value="MS">Montserrat</option>
<option value="MA">Morocco</option>
<option value="MZ">Mozambique</option>
<option value="MM">Myanmar</option>
<option value="NA">Namibia</option>
<option value="NR">Nauru</option>
<option value="NP">Nepal</option>
<option value="NL">Netherlands</option>
<option value="NC">New Caledonia</option>
<option value="NZ">New Zealand</option>
<option value="NI">Nicaragua</option>
<option value="NE">Niger</option>
<option value="NG">Nigeria</option>
<option value="NU">Niue</option>
<option value="NF">Norfolk Island</option>
<option value="MP">Northern Mariana Islands</option>
<option value="NO">Norway</option>
<option value="OM">Oman</option>
<option value="PK">Pakistan</option>
<option value="PW">Palau</option>
<option value="PS">Palestine, State of</option>
<option value="PA">Panama</option>
<option value="PG">Papua New Guinea</option>
<option value="PY">Paraguay</option>
<option value="PE">Peru</option>
<option value="PH">Philippines</option>
<option value="PN">Pitcairn</option>
<option value="PL">Poland</option>
<option value="PT">Portugal</option>
<option value="PR">Puerto Rico</option>
<option value="QA">Qatar</option>
<option value="RE">Réunion</option>
<option value="RO">Romania</option>
<option value="RU">Russian Federation</option>
<option value="RW">Rwanda</option>
<option value="BL">Saint Barthélemy</option>
<option value="SH">Saint Helena, Ascension and Tristan da Cunha</option>
<option value="KN">Saint Kitts and Nevis</option>
<option value="LC">Saint Lucia</option>
<option value="MF">Saint Martin (French part)</option>
<option value="PM">Saint Pierre and Miquelon</option>
<option value="VC">Saint Vincent and the Grenadines</option>
<option value="WS">Samoa</option>
<option value="SM">San Marino</option>
<option value="ST">Sao Tome and Principe</option>
<option value="SA">Saudi Arabia</option>
<option value="SN">Senegal</option>
<option value="RS">Serbia</option>
<option value="SC">Seychelles</option>
<option value="SL">Sierra Leone</option>
<option value="SG">Singapore</option>
<option value="SX">Sint Maarten (Dutch part)</option>
<option value="SK">Slovakia</option>
<option value="SI">Slovenia</option>
<option value="SB">Solomon Islands</option>
<option value="SO">Somalia</option>
<option value="ZA">South Africa</option>
<option value="GS">South Georgia and the South Sandwich Islands</option>
<option value="ES">Spain</option>
<option value="LK">Sri Lanka</option>
<option value="SD">Sudan</option>
<option value="SR">Suriname</option>
<option value="SS">South Sudan</option>
<option value="SJ">Svalbard and Jan Mayen</option>
<option value="SZ">Swaziland</option>
<option value="SE">Sweden</option>
<option value="CH">Switzerland</option>
<option value="SY">Syrian Arab Republic</option>
<option value="TW">Taiwan</option>
<option value="TJ">Tajikistan</option>
<option value="TZ">Tanzania</option>
<option value="TH">Thailand</option>
<option value="TL">Timor-Leste</option>
<option value="TG">Togo</option>
<option value="TK">Tokelau</option>
<option value="TO">Tonga</option>
<option value="TT">Trinidad and Tobago</option>
<option value="TN">Tunisia</option>
<option value="TR">Turkey</option>
<option value="TM">Turkmenistan</option>
<option value="TC">Turks and Caicos Islands</option>
<option value="TV">Tuvalu</option>
<option value="UG">Uganda</option>
<option value="UA">Ukraine</option>
<option value="AE">United Arab Emirates</option>
<option value="GB">United Kingdom</option>
<option value="US" selected="selected">United States</option>
<option value="UM">United States Minor Outlying Islands</option>
<option value="UY">Uruguay</option>
<option value="UZ">Uzbekistan</option>
<option value="VU">Vanuatu</option>
<option value="VE">Venezuela</option>
<option value="VN">Viet Nam</option>
<option value="VG">Virgin Islands, British</option>
<option value="VI">Virgin Islands, U.S.</option>
<option value="WF">Wallis and Futuna</option>
<option value="EH">Western Sahara</option>
<option value="YE">Yemen</option>
<option value="ZM">Zambia</option>
<option value="ZW">Zimbabwe</option>
</select><script>
$('#country_selector_quote-1784').selectize();
</script><br />
</div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">State</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][state]" id="state-1784" maxlength="3" type="text"/><br />
</div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Email <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][email]" placeholder="email@address.com" maxlength="255" type="email" id="QuoteEmail" required="required"/> </div>
</div>
<div class="row collapse" id="email_v">
<div class="small-3 large-2 columns">
<span class="prefix">Email verification<sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][email_v]" autocomplete="nope" type="text" id="QuoteEmailV"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Comment</span>
</div>
<div class="small-9 large-10 columns">
<textarea name="data[Quote][comment]" placeholder="Additional comments" cols="30" rows="6" id="QuoteComment"></textarea> </div>
</div>
<!------------SERVICES PARTICULAR FORM START---------------->
<!------------DATA TO POPULATE REGARDING SPECIFIC SERVICES----->
<div class="row collapse">
<div class="small-3 large-2 columns">
</div>
<div class="small-9 large-10 columns">
<div class="recaptcha"><div id="recaptcha662261cea3660"></div></div> </div>
</div>
<br />
<div class="row collapse">
<div class="small-3 large-2 columns">
</div>
<div class="small-9 large-10 columns">
<button id="submit_btn-1784" class="alert button expand" form="Quote-1784" type="submit">Contact me</button> </div>
</div>
</form><script>
var pardotFormHandlerURL = 'https://go.diagenode.com/l/928883/2022-10-10/36b1c';
function postToPardot(formAction, id) {
$('#pardot-form-handler').load(function(){
$('#Quote-' + id).attr('action', formAction);
$('#Quote-' + id).submit();
});
$('#pardot-form-handler').attr('src', pardotFormHandlerURL + '?' + $('#Quote-' + id).serialize());
}
$(document).ready(function() {
$('body').append('<iframe id="pardot-form-handler" height="0" width="0" style="position:absolute; left:-100000px; top:-100000px" src="javascript:false;"></iframe>');
$('#Quote-1784').attr('action','javascript:postToPardot(\'' + $('#Quote-1784').attr('action') + '\', 1784)');
});
$("#Quote-1784 #submit_btn-1784").click(function (e) {
if($(this).closest('form')[0].checkValidity()){
e.preventDefault();
//disable the submit button
$("#Quote-1784 #submit_btn-1784").attr("disabled", true);
$("#Quote-1784 #submit_btn-1784").html("Processing...");
//submit the form
$("#Quote-1784").submit();
}
})
</script>
<script>
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$("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="AL">Alabama (AL)</option><option value="AK">Alaska (AK)</option><option value="AZ">Arizona (AZ)</option><option value="AR">Arkansas (AR)</option><option value="CA">California (CA)</option><option value="CO">Colorado (CO)</option><option value="CT">Connecticut (CT)</option><option value="DE">Delaware (DE)</option><option value="FL">Florida (FL)</option><option value="GA">Georgia (GA)</option><option value="HI">Hawaii (HI)</option><option value="ID">Idaho (ID)</option><option value="IL">Illinois (IL)</option><option value="IN">Indiana (IN)</option><option value="IA">Iowa (IA)</option><option value="KS">Kansas (KS)</option><option value="KY">Kentucky (KY)</option><option value="LA">Louisiana (LA)</option><option value="ME">Maine (ME)</option><option value="MD">Maryland (MD)</option><option value="MA">Massachusetts (MA)</option><option value="MI">Michigan (MI)</option><option value="MN">Minnesota (MN)</option><option value="MS">Mississippi (MS)</option><option value="MO">Missouri (MO)</option><option value="MT">Montana (MT)</option><option value="NE">Nebraska (NE)</option><option value="NV">Nevada (NV)</option><option value="NH">New Hampshire (NH)</option><option value="NJ">New Jersey (NJ)</option><option value="NM">New Mexico (NM)</option><option value="NY">New York (NY)</option><option value="NC">North Carolina (NC)</option><option value="ND">North Dakota (ND)</option><option value="OH">Ohio (OH)</option><option value="OK">Oklahoma (OK)</option><option value="OR">Oregon (OR)</option><option value="PA">Pennsylvania (PA)</option><option value="PR">Puerto Rico (PR)</option><option value="RI">Rhode Island (RI)</option><option value="SC">South Carolina (SC)</option><option value="SD">South Dakota (SD)</option><option value="TN">Tennessee (TN)</option><option value="TX">Texas (TX)</option><option value="UT">Utah (UT)</option><option value="VT">Vermont (VT)</option><option value="VA">Virginia (VA)</option><option value="WA">Washington (WA)</option><option value="WV">West Virginia (WV)</option><option value="WI">Wisconsin (WI)</option><option value="WY">Wyoming (WY)</option><option value="DC">District of Columbia (DC)</option><option value="AS">American Samoa (AS)</option><option value="GU">Guam (GU)</option><option value="MP">Northern Mariana Islands (MP)</option><option value="PR">Puerto Rico (PR)</option><option value="UM">United States Minor Outlying Islands (UM)</option><option value="VI">Virgin Islands (VI)</option></select>');
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$("#Quote-1784 #state-1784").replaceWith('<select name="data[Quote][state]" id="state-1784" required><option disabled selected value> -- select a state -- </option><option value="AB">Alberta (AB)</option><option value="BC">British Columbia (BC)</option><option value="MB">Manitoba (MB)</option><option value="NB">New Brunswick (NB)</option><option value="NL">Newfoundland and Labrador (NL)</option><option value="NS">Nova Scotia (NS)</option><option value="ON">Ontario (ON)</option><option value="PE">Prince Edward Island (PE)</option><option value="QC">Quebec (QC)</option><option value="SK">Saskatchewan (SK)</option></select>');
if (val.length == 2) {
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<a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: QUOTE MODAL --><a href="#" id="bioruptor-plus-sonication-device" data-reveal-id="quoteModal-1784" class="quote_btn" style="color:#B21329"><i class="fa fa-info-circle"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Plus sonication device</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/protein-extraction-kit-50-rxns"><img src="/img/product/kits/kit-icon.png" alt="Kit icon" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C20000020</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2617" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2617" id="CartAdd/2617Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2617" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> Protein Extraction kit for Bioruptor<sup>®</sup> Plus</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
'C20000020',
'435',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('Protein Extraction kit for Bioruptor<sup>®</sup> Plus',
'C20000020',
'435',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="protein-extraction-kit-50-rxns" data-reveal-id="cartModal-2617" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">Protein Extraction kit for Bioruptor® Plus</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/15-ml-tpx-tubes-50-pc"><img src="/img/product/shearing_technologies/C30010009_tube.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C30010009</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2621" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2621" id="CartAdd/2621Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2621" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor<sup>®</sup> Plus TPX tubes</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'C30010009',
'120',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Plus TPX tubes',
'C30010009',
'120',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-tpx-tubes-50-pc" data-reveal-id="cartModal-2621" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Plus TPX tubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/15-ml-bioruptor-tubes-50-pc"><img src="/img/product/shearing_technologies/C30010017_tube.png" alt="15 ml tubes" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C30010017</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-2631" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/2631" id="CartAdd/2631Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="2631" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor® Pico Tubes</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor® Pico Tubes',
'C30010017',
'115',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor® Pico Tubes',
'C30010017',
'115',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-bioruptor-tubes-50-pc" data-reveal-id="cartModal-2631" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Pico Tubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns"><img src="/img/product/shearing_technologies/C01020031_tube.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">C01020031</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: ADD TO CART MODAL --><div id="cartModal-1875" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<form action="/en/carts/add/1875" id="CartAdd/1875Form" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Cart][product_id]" value="1875" id="CartProductId"/>
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor<sup>®</sup> Pico Tubes & sonication beads</strong> to my shopping cart.</p>
<div class="row">
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Pico Tubes & sonication beads',
'C01020031',
'225',
$('#CartQuantity').val());" name="checkout" id="checkout" value="checkout" type="submit">Checkout</button> </div>
<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Pico Tubes & sonication beads',
'C01020031',
'225',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-bioruptor-tubes-sonication-beads-50-rxns" data-reveal-id="cartModal-1875" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Pico Tubes & sonication beads</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/bioruptorpico2"><img src="/img/product/shearing_technologies/B01080000-1.jpg" alt="Bioruptor Pico" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
<span class="success label" style="">B01080010</span>
</div>
<div class="small-6 columns text-right" style="padding-left:0px;padding-right:0px;margin-top:-6px">
<!--a href="#" style="color:#B21329"><i class="fa fa-info-circle"></i></a-->
<!-- BEGIN: QUOTE MODAL --><div id="quoteModal-3046" class="reveal-modal small" data-reveal aria-labelledby="modalTitle" aria-hidden="true" role="dialog">
<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Bioruptor<sup>®</sup> Pico sonication device</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>
</div>
</div>
<form action="/en/quotes/quote?id=3046" id="Quote-3046" class="quote" method="post" accept-charset="utf-8"><div style="display:none;"><input type="hidden" name="_method" value="POST"/></div><input type="hidden" name="data[Quote][product_id]" value="3046" id="QuoteProductId"/><input type="hidden" name="data[Quote][formLoaded6tY4bPYk]" value="MExEWnYraStBcHRxZXNQbTVmZ0luQT09" id="QuoteFormLoaded6tY4bPYk"/><input type="hidden" name="data[Quote][product_rfq_tag]" value="bioruptorpico2" id="QuoteProductRfqTag"/><input type="hidden" name="data[Quote][source_quote]" value="modal quote" id="QuoteSourceQuote"/>
<div class="row collapse">
<h2>Contact Information</h2>
<div class="small-3 large-2 columns">
<span class="prefix">First name <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][first_name]" placeholder="john" maxlength="255" type="text" id="QuoteFirstName" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Last name <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][last_name]" placeholder="doe" maxlength="255" type="text" id="QuoteLastName" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Company <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][company]" placeholder="Organisation / Institute" maxlength="255" type="text" id="QuoteCompany" required="required"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Phone number</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][phone_number]" placeholder="+1 862 209-4680" maxlength="255" type="text" id="QuotePhoneNumber"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">City</span>
</div>
<div class="small-9 large-10 columns">
<input name="data[Quote][city]" placeholder="Denville" maxlength="255" type="text" id="QuoteCity"/> </div>
</div>
<div class="row collapse">
<div class="small-3 large-2 columns">
<span class="prefix">Country <sup style="font-size:16px;color:red;">*</sup></span>
</div>
<div class="small-9 large-10 columns">
<select name="data[Quote][country]" required="required" class="triggers" id="country_selector_quote-3046">
<option value="">-- select a country --</option>
<option value="AF">Afghanistan</option>
<option value="AX">Åland Islands</option>
<option value="AL">Albania</option>
<option value="DZ">Algeria</option>
<option value="AS">American Samoa</option>
<option value="AD">Andorra</option>
<option value="AO">Angola</option>
<option value="AI">Anguilla</option>
<option value="AQ">Antarctica</option>
<option value="AG">Antigua and Barbuda</option>
<option value="AR">Argentina</option>
<option value="AM">Armenia</option>
<option value="AW">Aruba</option>
<option value="AU">Australia</option>
<option value="AT">Austria</option>
<option value="AZ">Azerbaijan</option>
<option value="BS">Bahamas</option>
<option value="BH">Bahrain</option>
<option value="BD">Bangladesh</option>
<option value="BB">Barbados</option>
<option value="BY">Belarus</option>
<option value="BE">Belgium</option>
<option value="BZ">Belize</option>
<option value="BJ">Benin</option>
<option value="BM">Bermuda</option>
<option value="BT">Bhutan</option>
<option value="BO">Bolivia</option>
<option value="BQ">Bonaire, Sint Eustatius and Saba</option>
<option value="BA">Bosnia and Herzegovina</option>
<option value="BW">Botswana</option>
<option value="BV">Bouvet Island</option>
<option value="BR">Brazil</option>
<option value="IO">British Indian Ocean Territory</option>
<option value="BN">Brunei Darussalam</option>
<option value="BG">Bulgaria</option>
<option value="BF">Burkina Faso</option>
<option value="BI">Burundi</option>
<option value="KH">Cambodia</option>
<option value="CM">Cameroon</option>
<option value="CA">Canada</option>
<option value="CV">Cape Verde</option>
<option value="KY">Cayman Islands</option>
<option value="CF">Central African Republic</option>
<option value="TD">Chad</option>
<option value="CL">Chile</option>
<option value="CN">China</option>
<option value="CX">Christmas Island</option>
<option value="CC">Cocos (Keeling) Islands</option>
<option value="CO">Colombia</option>
<option value="KM">Comoros</option>
<option value="CG">Congo</option>
<option value="CD">Congo, The Democratic Republic of the</option>
<option value="CK">Cook Islands</option>
<option value="CR">Costa Rica</option>
<option value="CI">Côte d'Ivoire</option>
<option value="HR">Croatia</option>
<option value="CU">Cuba</option>
<option value="CW">Curaçao</option>
<option value="CY">Cyprus</option>
<option value="CZ">Czech Republic</option>
<option value="DK">Denmark</option>
<option value="DJ">Djibouti</option>
<option value="DM">Dominica</option>
<option value="DO">Dominican Republic</option>
<option value="EC">Ecuador</option>
<option value="EG">Egypt</option>
<option value="SV">El Salvador</option>
<option value="GQ">Equatorial Guinea</option>
<option value="ER">Eritrea</option>
<option value="EE">Estonia</option>
<option value="ET">Ethiopia</option>
<option value="FK">Falkland Islands (Malvinas)</option>
<option value="FO">Faroe Islands</option>
<option value="FJ">Fiji</option>
<option value="FI">Finland</option>
<option value="FR">France</option>
<option value="GF">French Guiana</option>
<option value="PF">French Polynesia</option>
<option value="TF">French Southern Territories</option>
<option value="GA">Gabon</option>
<option value="GM">Gambia</option>
<option value="GE">Georgia</option>
<option value="DE">Germany</option>
<option value="GH">Ghana</option>
<option value="GI">Gibraltar</option>
<option value="GR">Greece</option>
<option value="GL">Greenland</option>
<option value="GD">Grenada</option>
<option value="GP">Guadeloupe</option>
<option value="GU">Guam</option>
<option value="GT">Guatemala</option>
<option value="GG">Guernsey</option>
<option value="GN">Guinea</option>
<option value="GW">Guinea-Bissau</option>
<option value="GY">Guyana</option>
<option value="HT">Haiti</option>
<option value="HM">Heard Island and McDonald Islands</option>
<option value="VA">Holy See (Vatican City State)</option>
<option value="HN">Honduras</option>
<option value="HK">Hong Kong</option>
<option value="HU">Hungary</option>
<option value="IS">Iceland</option>
<option value="IN">India</option>
<option value="ID">Indonesia</option>
<option value="IR">Iran, Islamic Republic of</option>
<option value="IQ">Iraq</option>
<option value="IE">Ireland</option>
<option value="IM">Isle of Man</option>
<option value="IL">Israel</option>
<option value="IT">Italy</option>
<option value="JM">Jamaica</option>
<option value="JP">Japan</option>
<option value="JE">Jersey</option>
<option value="JO">Jordan</option>
<option value="KZ">Kazakhstan</option>
<option value="KE">Kenya</option>
<option value="KI">Kiribati</option>
<option value="KP">Korea, Democratic People's Republic of</option>
<option value="KR">Korea, Republic of</option>
<option value="KW">Kuwait</option>
<option value="KG">Kyrgyzstan</option>
<option value="LA">Lao People's Democratic Republic</option>
<option value="LV">Latvia</option>
<option value="LB">Lebanon</option>
<option value="LS">Lesotho</option>
<option value="LR">Liberia</option>
<option value="LY">Libya</option>
<option value="LI">Liechtenstein</option>
<option value="LT">Lithuania</option>
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<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Pico sonication device</h6>
</div>
</div>
</li>
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'id' => '3046',
'antibody_id' => null,
'name' => 'Bioruptor<sup>®</sup> Pico sonication device',
'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p>
<p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p>
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<p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p>
<p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p>
<p><strong>Application versatility</strong>:</p>
<ul>
<li>DNA shearing for Next-Generation-Sequencing</li>
<li>Chromatin shearing</li>
<li>RNA shearing</li>
<li>Protein extraction from tissues and cells (also for mass spectrometry)</li>
<li>FFPE DNA extraction</li>
<li>Protein aggregation studies</li>
<li>CUT&RUN - shearing of input DNA for NGS</li>
</ul>
<div style="background-color: #f1f3f4; margin: 10px; padding: 50px;">
<p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p>
</div>
<div class="extra-spaced"><img alt="Bioruptor Sonication for DNA shearing for Next-Generation-Sequencing" src="https://www.diagenode.com/img/product/shearing_technologies/guarantie.png" /></div>
<div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div>
<div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div>
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'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico',
'info2' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
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<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
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<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
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<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
'label3' => 'Available shearing Kits',
'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p>
<p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p>
<h2>Kit choice guide</h2>
<table style="border: 0;" valign="center">
<tbody>
<tr style="background: #fff;">
<th class="text-center"></th>
<th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th>
<th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th>
<th class="text-center" style="font-size: 17px;">KIT</th>
<th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th>
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<td class="text-center" style="border-bottom: 1px solid #dedede;">
<div class="label alert" style="font-size: 17px;">CELLS</div>
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<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td>
<td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px;">> 100,000</td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">PLANT TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td>
<td class="text-center" style="font-size: 17px;">0.5%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">0.2%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td colspan="7"></td>
</tr>
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<td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td>
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center"></td>
<td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td>
<td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
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<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
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<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
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<div class="extra-spaced">
<h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3>
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'price_USD' => '350',
'price_GBP' => '250',
'price_JPY' => '46995',
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'id' => '1784',
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'name' => 'Bioruptor<sup>®</sup> Plus sonication device',
'description' => '<p><a href="https://go.diagenode.com/bioruptor-upgrade"><img src="https://www.diagenode.com/img/banners/banner-br-trade.png" /></a></p>
<div class="row">
<div class="small-12 medium-12 large-12 columns"><br /><br />
<p><span>The Bioruptor® uses a unique system to uniformely process multiple samples in sealed tubes of 0.5 ml to 50 ml capacity. The built-in cooling system (water cooler and Single Cycle Valve) ensures high precision temperature control resulting in higher quality samples. An excellent device for shearing chromatin, cell and tissue disruption and many other applications (see below).</span></p>
</div>
</div>
<p></p>
<p><span></span></p>
<div class="page" title="Page 9">
<div class="section">
<div class="layoutArea">
<div class="column"></div>
</div>
</div>
</div>',
'label1' => 'Which Bioruptor® Plus configuration is right for you?',
'info1' => '<p><strong>B01020001</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve) </span><em>for recommended sample volume 100 µl - 300 µl</em></p>
<p><strong>B01020002</strong> - Bioruptor Plus<sup>®</sup> device with 1.5 ml (6 samples) and 15 ml tube holders <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 100 µl - 2 ml</em></p>
<p><strong>B01020003</strong> - Bioruptor Plus<sup>®</sup> device with 0.5 ml (12 samples) tube holder <span>& temperature controlled system (water cooler + single cycle valve)</span> <em>for recommended sample volume 50 µl - 100 µl </em></p>',
'label2' => 'Available chromatin shearing kits',
'info2' => '<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histones)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="../p/chromatin-shearing-plant-chip-seq-kit">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">< 0.1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.2%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">1%</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">0.5%</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">No</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">Yes</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">100 million cells</p>
</td>
<td style="text-align: center;">
<p style="text-align: center;">up to 25 g of tissue</p>
</td>
</tr>
<tr style="background-color: #fff;" valign="middle">
<td>
<p style="text-align: left;"><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
<p style="text-align: center;"><a href="https://www.diagenode.com/en/p/manual-chipmentation-kit-for-histones-24-rxns">ChIPmentation Kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p style="text-align: center;"><a href="../p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p style="text-align: center;"><a href="../p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant <br />ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>',
'label3' => 'View accessories & consumables for Bioruptor<sup>®</sup> Plus',
'info3' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-5-0-65-ml-tube-holder-for-bioruptor-standard-plus-pico-1-pack">0.5/0.65 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200043</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">1.5 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200011</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/10-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">10 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01200012</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">15 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200013</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/50-ml-tube-holder-for-bioruptor-standard-bioruptor-plus-1-pack">50 ml tube holder</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200014</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">3 samples</span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-5-ml-bioruptor-microtubes-500-tubes">0.5 ml Bioruptor Plus Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010013</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tpx-microtubes-300-pc">1.5 ml Bioruptor Plus TPX microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010010</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/10-ml-tubes-100-tubes">10 ml Bioruptor Plus tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010012</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-tpx-tubes-50-pc">15 ml Bioruptor Plus TPX tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010009</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
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<p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p>
<div class="row">
<div class="small-12 medium-8 large-8 columns"><br />
<p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p>
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<p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p>
<p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p>
<p><strong>Application versatility</strong>:</p>
<ul>
<li>DNA shearing for Next-Generation-Sequencing</li>
<li>Chromatin shearing</li>
<li>RNA shearing</li>
<li>Protein extraction from tissues and cells (also for mass spectrometry)</li>
<li>FFPE DNA extraction</li>
<li>Protein aggregation studies</li>
<li>CUT&RUN - shearing of input DNA for NGS</li>
</ul>
<div style="background-color: #f1f3f4; margin: 10px; padding: 50px;">
<p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p>
</div>
<div class="extra-spaced"><img alt="Bioruptor Sonication for DNA shearing for Next-Generation-Sequencing" src="https://www.diagenode.com/img/product/shearing_technologies/guarantie.png" /></div>
<div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div>
<div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div>
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'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico',
'info2' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
</thead>
<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
'label3' => 'Available shearing Kits',
'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p>
<p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p>
<h2>Kit choice guide</h2>
<table style="border: 0;" valign="center">
<tbody>
<tr style="background: #fff;">
<th class="text-center"></th>
<th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th>
<th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th>
<th class="text-center" style="font-size: 17px;">KIT</th>
<th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th>
<th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th>
</tr>
<tr style="background: #fff;">
<td colspan="7"></td>
</tr>
<tr style="background: #fff;">
<td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td>
<td class="text-center" style="border-bottom: 1px solid #dedede;">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td>
<td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px;">> 100,000</td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">PLANT TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td>
<td class="text-center" style="font-size: 17px;">0.5%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">0.2%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td colspan="7"></td>
</tr>
<tr style="background: #fff;">
<td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td>
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center"></td>
<td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td>
<td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
</td>
<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
</tr>
</tbody>
</table>
<div class="extra-spaced">
<h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3>
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'slug' => 'bioruptorpico2',
'meta_title' => 'Bioruptor® Pico sonication device',
'meta_keywords' => '',
'meta_description' => 'Bioruptor® Pico sonication device',
'modified' => '2023-06-20 18:16:03',
'created' => '2020-01-09 16:27:17',
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'Application' => array(
(int) 0 => array(
'id' => '8',
'position' => '10',
'parent_id' => null,
'name' => 'Chromatin shearing',
'description' => '<div class="row">
<div class="small-12 medium-12 large-12 columns">The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, <strong>Diagenode's Bioruptor</strong><sup>®</sup> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) to efficiently shear chromatin. ACT enables the highest chromatin quality for high IP efficiency and sensitivity for ChIP experiments with gentle yet highly effective shearing forces. Additionally, the Bioruptor<sup>®</sup> provides a precisely controlled temperature environment that preserves chromatin from heat degradation such that protein-DNA complexes are well-preserved for sensitive, unbiased, and accurate ChIP.<br /><br /> <strong>Diagenode's Bioruptor</strong><sup>®</sup> is the instrument of choice for chromatin shearing used for a number of downstream applications such as qPCR and ChIP-seq that require optimally sheared, unbiased chromatin.</div>
<div class="small-12 medium-12 large-12 columns text-center"><br /><img src="https://www.diagenode.com/img/applications/pico_dna_shearing_fig2.png" /></div>
<div class="small-10 medium-10 large-10 columns end small-offset-1"><small> <br /><strong>Panel A, 10 µl volume:</strong> Chromatin samples are sheared for 10, 20 and 30 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.1 ml Bioruptor® Microtubes (Cat. No. B01200041). <strong>Panel B, 100 µl volume:</strong> Chromatin samples are sheared for 10 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using 0.65 ml Bioruptor® Microtubes (Cat. No. WA-005-0500). <strong>Panel C, 300 µl volume:</strong> Chromatin samples are sheared for 5, 10 and 15 cycles of 30 sec ON/30 sec OFF with the Bioruptor® Pico using using 1.5 ml Bioruptor microtubes (Cat. No. C30010016). Prior to de-crosslinking, samples are treated with RNase cocktail mixture at 37°C during 1 hour. The sheared chromatin is then de-crosslinked overnight and phenol/chloroform purified as described in the kit manual. 10 µl of DNA (equivalent of 500, 000 cells) are analyzed on a 2% agarose gel (MW corresponds to the 100 bp DNA molecular weight marker).</small></div>
<div class="small-12 medium-12 large-12 columns"><br /><br /></div>
<div class="small-12 medium-12 large-12 columns">
<p>It is important to establish optimal conditions to shear crosslinked chromatin to get the correct fragment sizes needed for ChIP. Usually this process requires both optimizing sonication conditions as well as optimizing SDS concentration, which is laborious. With the Chromatin Shearing Optimization Kits, optimization is fast and easy - we provide optimization reagents with varying concentrations of SDS. Moreover, our Chromatin Shearing Optimization Kits can be used for the optimization of chromatin preparation with our kits for ChIP.</p>
</div>
<div class="small-12 medium-12 large-12 columns">
<div class="page" title="Page 7">
<table>
<tbody>
<tr valign="middle">
<td></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-100-million-cells">Chromatin Shearing Kit Low SDS (for Histone)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-low-sds-for-tfs-25-rxns">Chromatin Shearing Kit Low SDS (for TF)</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin Shearing Kit High SDS</a></strong></td>
<td style="text-align: center;"><strong><a href="https://www.diagenode.com/p/chromatin-shearing-optimization-kit-medium-sds-100-million-cells">Chromatin Shearing Kit (for Plant)</a></strong></td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>SDS concentration</strong></p>
</td>
<td style="text-align: center;">
<p>< 0.1%</p>
</td>
<td style="text-align: center;">
<p>0.2%</p>
</td>
<td style="text-align: center;">
<p>1%</p>
</td>
<td style="text-align: center;">
<p>0.5%</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Nuclei isolation</strong></p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
<td style="text-align: center;">
<p>No</p>
</td>
<td style="text-align: center;">
<p>Yes</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Allows for shearing of... cells/tissue</strong></p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>100 million cells</p>
</td>
<td style="text-align: center;">
<p>up to 25 g of tissue</p>
</td>
</tr>
<tr valign="middle" style="background-color: #fff;">
<td>
<p><strong>Corresponding to shearing buffers from</strong></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-x24-24-rxns">iDeal ChIP-seq kit for Histones</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/ideal-chip-seq-kit-for-transcription-factors-x24-24-rxns">iDeal ChIP-seq Kit for Transcription Factors</a></p>
<p><a href="https://www.diagenode.com/en/p/ideal-chip-qpcr-kit">iDeal ChIP qPCR kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/true-microchip-kit-x16-16-rxns">True MicroChIP kit</a></p>
</td>
<td style="text-align: center;">
<p><a href="https://www.diagenode.com/en/p/universal-plant-chip-seq-kit-x24-24-rxns">Universal Plant ChIP-seq kit</a></p>
</td>
</tr>
</tbody>
</table>
<p><em><span style="font-weight: 400;">Table comes from our </span><a href="https://www.diagenode.com/protocols/bioruptor-pico-chromatin-preparation-guide"><span style="font-weight: 400;">Guide for successful chromatin preparation using the Bioruptor® Pico</span></a></em></p>
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'meta_title' => 'Chromatin shearing using Bioruptor® sonication device | Diagenode',
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<div class="large-12 columns">Various biochemical and analytical techniques require the extraction of RNA from tissues. Isolating intact RNA is essential for many techniques used in gene expression analysis. In order to obtain optimal yields of RNA, the efficient disruption and homogenization of tissues and cultured cells are required. <strong>Diagenode's Bioruptor</strong><sup>® </sup><strong>Plus</strong> uses state-of-the-art ultrasound <strong>ACT</strong> (<strong>A</strong>daptive <strong>C</strong>avitation <strong>T</strong>echnology) combined with unique devices such as beads or tubes acting as sonication « enhancers » or dedicated reagents (such as our RNA extraction reagent) to efficiently disrupt tissues and cultured cells in just one step to deliver high quality RNA extraction.</div>
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<div class="small-12 medium-12 large-12 columns text-center"><img src="https://www.diagenode.com/img/applications/pro-rna-extract-fig3a.png" /></div>
<div class="small-6 medium-6 large-6 columns text-center end small-offset-3"><small><strong>Efficient extraction of pure RNA with high RIN. </strong><br /><span>Total RNA profiles from mouse brain. Tissue was disrupted with Bioruptor<sup>®</sup> Plus as described in the protocol and analyzed on BioAnalyzer (Agilent). Note that small RNAs are present in all profiles indicating that the RNA is largely intact.</span></small></div>
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<h6 style="height:60px">Protein Extraction kit for Bioruptor® Plus</h6>
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<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor<sup>®</sup> Plus TPX tubes</strong> to my shopping cart.</p>
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<h6 style="height:60px">15 ml Bioruptor® Plus TPX tubes</h6>
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<li>
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<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor® Pico Tubes',
'C30010017',
'115',
$('#CartQuantity').val());" name="keepshop" id="keepshop" type="submit">Keep shopping</button> </div>
</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-bioruptor-tubes-50-pc" data-reveal-id="cartModal-2631" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
</div>
</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Pico Tubes</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns"><img src="/img/product/shearing_technologies/C01020031_tube.png" alt="some alt" class="th"/></a> </div>
<div class="small-12 columns">
<div class="small-6 columns" style="padding-left:0px;padding-right:0px;margin-top:-6px;margin-left:-1px">
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<p>Add <input name="data[Cart][quantity]" placeholder="1" value="1" min="1" style="width:60px;display:inline" type="number" id="CartQuantity" required="required"/> <strong> 15 ml Bioruptor<sup>®</sup> Pico Tubes & sonication beads</strong> to my shopping cart.</p>
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<div class="small-6 medium-6 large-6 columns">
<button class="alert small button expand" onclick="$(this).addToCart('15 ml Bioruptor<sup>®</sup> Pico Tubes & sonication beads',
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</div>
</div>
</div>
</form><a class="close-reveal-modal" aria-label="Close">×</a></div><!-- END: ADD TO CART MODAL --><a href="#" id="15-ml-bioruptor-tubes-sonication-beads-50-rxns" data-reveal-id="cartModal-1875" class="" style="color:#B21329"><i class="fa fa-cart-plus"></i></a>
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</div>
<div class="small-12 columns" >
<h6 style="height:60px">15 ml Bioruptor® Pico Tubes & sonication beads</h6>
</div>
</div>
</li>
<li>
<div class="row">
<div class="small-12 columns">
<a href="/en/p/bioruptorpico2"><img src="/img/product/shearing_technologies/B01080000-1.jpg" alt="Bioruptor Pico" class="th"/></a> </div>
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<div class="row">
<div class="small-12 medium-12 large-12 columns">
<h3>Get a quote</h3><p class="lead">You are about to request a quote for <strong>Bioruptor<sup>®</sup> Pico sonication device</strong>. Fill out the form below and we will be in touch with you very soon.</p><p><small>All <span style="font-size:16px;color:red;">*</span> fields are mandatory</small></p>
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</div>
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<div class="small-12 columns" >
<h6 style="height:60px">Bioruptor® Pico sonication device</h6>
</div>
</div>
</li>
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'name' => 'Bioruptor<sup>®</sup> Pico sonication device',
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<p><a href="https://www.diagenode.com/en/categories/bioruptor-maintenance"><img src="https://www.diagenode.com/img/banners/maintenance-banner-br.png" /></a></p>
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<div class="small-12 medium-8 large-8 columns"><br />
<p><span>The Bioruptor® Pico is the latest innovation in shearing and represents a new breakthrough as an all-in-one shearing system capable of shearing samples from 150 bp to 1 kb. </span>Since 2004, Diagenode has accumulated <strong>shearing expertise</strong> to design the Bioruptor® Pico and guarantee the best experience with the <strong>sample preparation</strong> for <strong>number of applications -- in various fields of studies</strong> including environmental research, toxicology, genomics and epigenomics, cancer research, stem cells and development, neuroscience, clinical applications, agriculture, and many more.</p>
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<p>The Bioruptor Pico shearing accessories and consumables have been developed to allow <strong>flexibility in sample volumes</strong> (20 µl - 2 ml) and a <strong>fast parallel processing of samples</strong> (up to 16 samples simultaneously). <span>The built-in cooling system (Bioruptor® Cooler) ensures high precision <strong>temperature control</strong>. The <strong>user-friendly interface</strong> has been designed for any researcher, providing an easy and advanced modes that give both beginners and experienced users the right level of control. </span></p>
<p>In addition, Diagenode provides fully-validated tubes that remain <strong>budget-friendly with low operating cost</strong> (< 1€/$/DNA sample) and shearing kits for best sample quality. <span></span></p>
<p><strong>Application versatility</strong>:</p>
<ul>
<li>DNA shearing for Next-Generation-Sequencing</li>
<li>Chromatin shearing</li>
<li>RNA shearing</li>
<li>Protein extraction from tissues and cells (also for mass spectrometry)</li>
<li>FFPE DNA extraction</li>
<li>Protein aggregation studies</li>
<li>CUT&RUN - shearing of input DNA for NGS</li>
</ul>
<div style="background-color: #f1f3f4; margin: 10px; padding: 50px;">
<p><strong>Bioruptor Pico: Recommended for CUT&RUN sequencing for input DNA</strong><br /><br /> By combining antibody-targeted controlled cleavage by MNase and NGS, <strong>CUT&RUN sequencing</strong> can be used to identify protein-DNA binding sites genome-wide. CUT&RUN works by using the DNA cleaving activity of a Protein A-fused MNase to isolate DNA that is bound by a protein of interest. This targeted digestion is controlled by the addition of calcium, which MNase requires for its nuclease activity. After MNase digestion, short DNA fragments are released and can then be purified for subsequent library preparation and high-throughput sequencing. While CUT&RUN does not require mechanical shearing chromatin given the enzymatic approach, sonication is highly recommended for the fragmentation of the input DNA (used to compare the enriched sample) in order to be compatible with downstream NGS. The Bioruptor Pico is the ideal instrument of choice for generating optimal DNA fragments with a tight distribution, assuring excellent library prep and excellent sequencing results for your CUT&RUN assay.<br /><br /> <strong>Explore the Bioruptor Pico now.</strong></p>
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<div class="extra-spaced"><img alt="Bioruptor Sonication for DNA shearing for Next-Generation-Sequencing" src="https://www.diagenode.com/img/product/shearing_technologies/guarantie.png" /></div>
<div class="extra-spaced"><center><img alt="Bioruptor Sonication for Chromatin shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-reproducibility-is-priority.jpg" /></center></div>
<div class="extra-spaced"><center><a href="https://www.diagenode.com/en/pages/form-demo"> <img alt="Bioruptor Sonication for RNA shearing" src="https://www.diagenode.com/img/product/shearing_technologies/pico-request-demo.jpg" /></a></center></div>
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'label2' => 'View accessories & consumables for Bioruptor<sup>®</sup> Pico',
'info2' => '<h3>Shearing Accessories</h3>
<table style="width: 641px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 300px; height: 37px;"><strong>Name</strong></td>
<td style="width: 171px; text-align: center; height: 37px;">Catalog number</td>
<td style="width: 160px; text-align: center; height: 37px;">Throughput</td>
</tr>
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<tbody>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-2-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.2 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201144</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">16 samples</span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/0-65-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 0.65 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201143</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">12 samples<br /></span></td>
</tr>
<tr style="height: 38px;">
<td style="width: 300px; height: 38px;"><a href="https://www.diagenode.com/en/p/1-5-ml-tube-holder-dock-for-bioruptor-pico">Tube holder for 1.5 ml tubes</a></td>
<td style="width: 171px; text-align: center; height: 38px;"><span style="font-weight: 400;">B01201140</span></td>
<td style="width: 160px; text-align: center; height: 38px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 300px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-sonication-accessories-for-bioruptor-standard-plus-pico-1-pack">15 ml sonication accessories</a></td>
<td style="width: 171px; text-align: center; height: 37px;"><span style="font-weight: 400;">B01200016</span></td>
<td style="width: 160px; text-align: center; height: 37px;"><span style="font-weight: 400;">6 samples<br /></span></td>
</tr>
</tbody>
</table>
<h3>Shearing Consumables</h3>
<table style="width: 646px;">
<thead>
<tr style="background-color: #dddddd; height: 37px;">
<td style="width: 286px; height: 37px;"><strong>Name</strong></td>
<td style="width: 76px; height: 37px; text-align: center;">Catalog Number</td>
</tr>
</thead>
<tbody>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/02ml-microtubes-for-bioruptor-pico">0.2 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010020</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/0-65-ml-bioruptor-microtubes-500-tubes">0.65 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010011</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/1-5-ml-bioruptor-microtubes-with-caps-300-tubes">1.5 ml Pico Microtubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010016</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-50-pc">15 ml Pico Tubes</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C30010017</span></td>
</tr>
<tr style="height: 37px;">
<td style="width: 286px; height: 37px;"><a href="https://www.diagenode.com/en/p/15-ml-bioruptor-tubes-sonication-beads-50-rxns">15 ml Pico Tubes & sonication beads</a></td>
<td style="width: 76px; height: 37px; text-align: center;"><span style="font-weight: 400;">C01020031</span></td>
</tr>
</tbody>
</table>
<p><a href="https://www.diagenode.com/files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf">Find datasheet for Diagenode tubes here</a></p>
<p><a href="../documents/bioruptor-organigram-tubes">Which tubes for which Bioruptor®?</a></p>',
'label3' => 'Available shearing Kits',
'info3' => '<p>Diagenode has optimized a range of solutions for <strong>successful chromatin preparation</strong>. Chromatin EasyShear Kits together with the Pico ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.</p>
<p>For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs.</p>
<h2>Kit choice guide</h2>
<table style="border: 0;" valign="center">
<tbody>
<tr style="background: #fff;">
<th class="text-center"></th>
<th class="text-center" style="font-size: 17px;">SAMPLE TYPE</th>
<th class="text-center" style="font-size: 17px;">SAMPLE INPUT</th>
<th class="text-center" style="font-size: 17px;">KIT</th>
<th class="text-center" style="font-size: 17px;">SDS<br /> CONCENTRATION</th>
<th class="text-center" style="font-size: 17px;">NUCLEI<br /> ISOLATION</th>
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<tr style="background: #fff;">
<td colspan="7"></td>
</tr>
<tr style="background: #fff;">
<td rowspan="5"><img src="https://www.diagenode.com/img/label-histones.png" /></td>
<td class="text-center" style="border-bottom: 1px solid #dedede;">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">< 100,000</td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-optimization-kit-high-sds-100-million-cells">Chromatin EasyShear Kit<br />High SDS</a></td>
<td class="text-center" style="font-size: 17px; border-bottom: 1px solid #dedede;">1%</td>
<td class="text-center" style="border-bottom: 1px solid #dedede;"><img src="https://www.diagenode.com/img/cross-unvalid-green.jpg" width="18" height="20" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center" style="font-size: 17px;">> 100,000</td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-ultra-low-sds">Chromatin EasyShear Kit<br />Ultra Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">< 0.1%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff; border-bottom: 1px solid #dedede;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">PLANT TISSUE</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-shearing-plant-chip-seq-kit">Chromatin EasyShear Kit<br />for Plant</a></td>
<td class="text-center" style="font-size: 17px;">0.5%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
</td>
<td class="text-center"></td>
<td class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td class="text-center" style="font-size: 17px;">0.2%</td>
<td class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td colspan="7"></td>
</tr>
<tr style="background: #fff;">
<td rowspan="6"><img src="https://www.diagenode.com/img/label-tf.png" /></td>
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">CELLS</div>
</td>
<td class="text-center"></td>
<td rowspan="3" class="text-center" style="font-size: 17px;"><a href="https://www.diagenode.com/en/p/chromatin-easyshear-kit-low-sds">Chromatin EasyShear Kit<br />Low SDS</a></td>
<td rowspan="3" class="text-center" style="font-size: 17px;">0.2%</td>
<td rowspan="3" class="text-center"><img src="https://www.diagenode.com/img/valid.png" width="20" height="16" /></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">TISSUE</div>
</td>
<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td class="text-center">
<div class="label alert" style="font-size: 17px;">FFPE SAMPLES</div>
</td>
<td class="text-center"></td>
</tr>
<tr style="background: #fff;">
<td colspan="6"></td>
</tr>
</tbody>
</table>
<div class="extra-spaced">
<h3>Guide for optimal chromatin preparation using Chromatin EasyShear Kits <i class="fa fa-arrow-circle-right"></i> <a href="https://www.diagenode.com/pages/chromatin-prep-easyshear-kit-guide">Read more</a></h3>
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'slug' => 'bioruptorpico2',
'meta_title' => 'Bioruptor® Pico sonication device',
'meta_keywords' => '',
'meta_description' => 'Bioruptor® Pico sonication device',
'modified' => '2023-06-20 18:16:03',
'created' => '2020-01-09 16:27:17',
'ProductsRelated' => array(
'id' => '4592',
'product_id' => '1796',
'related_id' => '3046'
),
'Image' => array(
(int) 0 => array(
'id' => '1803',
'name' => 'product/shearing_technologies/B01080000-1.jpg',
'alt' => 'Bioruptor Pico',
'modified' => '2020-01-10 10:55:07',
'created' => '2020-01-10 10:52:54',
'ProductsImage' => array(
[maximum depth reached]
)
),
(int) 1 => array(
'id' => '1804',
'name' => 'product/shearing_technologies/B01080000-2.jpg',
'alt' => 'Bioruptor Pico',
'modified' => '2020-01-10 10:53:08',
'created' => '2020-01-10 10:53:08',
'ProductsImage' => array(
[maximum depth reached]
)
),
(int) 2 => array(
'id' => '1805',
'name' => 'product/shearing_technologies/B01080000-3.jpg',
'alt' => 'Bioruptor Pico',
'modified' => '2020-01-10 10:53:46',
'created' => '2020-01-10 10:53:46',
'ProductsImage' => array(
[maximum depth reached]
)
),
(int) 3 => array(
'id' => '1806',
'name' => 'product/shearing_technologies/B01080000-4.jpg',
'alt' => 'Bioruptor Pico',
'modified' => '2020-01-10 10:53:56',
'created' => '2020-01-10 10:53:56',
'ProductsImage' => array(
[maximum depth reached]
)
),
(int) 4 => array(
'id' => '1807',
'name' => 'product/shearing_technologies/B01080000-5.jpg',
'alt' => 'Bioruptor Pico',
'modified' => '2020-01-10 10:54:06',
'created' => '2020-01-10 10:54:06',
'ProductsImage' => array(
[maximum depth reached]
)
),
(int) 5 => array(
'id' => '1772',
'name' => 'product/shearing_technologies/B010600010.jpg',
'alt' => 'B010600010',
'modified' => '2018-02-14 15:41:46',
'created' => '2018-02-14 15:41:46',
'ProductsImage' => array(
[maximum depth reached]
)
)
)
)
$rrbs_service = array(
(int) 0 => (int) 1894,
(int) 1 => (int) 1895
)
$chipseq_service = array(
(int) 0 => (int) 2683,
(int) 1 => (int) 1835,
(int) 2 => (int) 1836,
(int) 3 => (int) 2684,
(int) 4 => (int) 1838,
(int) 5 => (int) 1839,
(int) 6 => (int) 1856
)
$labelize = object(Closure) {
}
$old_catalog_number = '<br/><small><span style="color:#CCC">(O-ring-15)</span></small>'
$country_code = 'US'
$label = '<img src="/img/banners/banner-customizer-back.png" alt=""/>'
$protocol = array(
'id' => '43',
'name' => 'TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®',
'description' => '<p><span>The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein complex. The fusion of the TAP tag to the protein of interest allows the rapid purification under a native environment. Molecular complexes can then be isolated and used for various applications for the identification of partners. Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes combined the TAP tagging with the Bioruptor</span><span>®</span><span>. This combination is essential for the efficient purification of a chromatin protein. </span></p>',
'image_id' => null,
'type' => 'Protocol',
'url' => 'files/protocols/TAP-TAG-procedure-for-bioruptor.pdf',
'slug' => 'tap-tag-procedure-for-bioruptor',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2016-04-29 17:47:40',
'created' => '2015-08-10 10:50:46',
'ProductsProtocol' => array(
'id' => '159',
'product_id' => '1796',
'protocol_id' => '43'
)
)
$document = array(
'id' => '1074',
'name' => 'Datasheet of Bioruptor tubes',
'description' => '<p>Datasheet of Diagenode tubes for Bioruptor Pico and Bioruptor Plus.</p>',
'image_id' => null,
'type' => 'Datasheet',
'url' => 'files/products/shearing_technology/bioruptor_accessories/TDS-BioruptorTubes.pdf',
'slug' => 'tds-bioruptor-tubes',
'meta_keywords' => '',
'meta_description' => '',
'modified' => '2022-02-23 12:21:44',
'created' => '2020-03-23 10:41:46',
'ProductsDocument' => array(
'id' => '3195',
'product_id' => '1796',
'document_id' => '1074'
)
)include - APP/View/Products/view.ctp, line 755
View::_evaluate() - CORE/Cake/View/View.php, line 971
View::_render() - CORE/Cake/View/View.php, line 933
View::render() - CORE/Cake/View/View.php, line 473
Controller::render() - CORE/Cake/Controller/Controller.php, line 963
ProductsController::slug() - APP/Controller/ProductsController.php, line 1052
ReflectionMethod::invokeArgs() - [internal], line ??
Controller::invokeAction() - CORE/Cake/Controller/Controller.php, line 491
Dispatcher::_invoke() - CORE/Cake/Routing/Dispatcher.php, line 193
Dispatcher::dispatch() - CORE/Cake/Routing/Dispatcher.php, line 167
[main] - APP/webroot/index.php, line 118
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