RNA extraction from tissue using Bioruptor® (Standard/Plus) and RNA extraction kit

Isolation of intact RNA is essential for many techniques used in gene expression analysis. Efficient disruption and homogenization of animal tissues are required to ensure high yield of RNA. Disruption releases RNA, while homogenization reduces sample viscosity to facilitate RNA purification. Diagenode’s Bioruptor® Sonicator uses state-of-the-art ultrasound technology to efficiently disrupt and homogenize tissues in one step. Diagenode’s RNA extraction reagent (included in the RNA extraction kit) is used as sonication medium and maintains the integrity of RNA while disrupting cells and dissolving cell components.

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