TAP-TAG - Procedure for the purification of a chromatin protein with Bioruptor®

The Tandem Affinity Purification (TAP) is a general procedure for the purification of protein complex. The fusion of the TAP tag to the protein of interest allows the rapid purification under a native environment. Molecular complexes can then be isolated and used for various applications for the identification of partners. Slimane AIT-SI-ALI and his “epigenetic and cell fate” team from the University Paris Descartes combined the TAP tagging with the Bioruptor®. This combination is essential for the efficient purification of a chromatin protein.

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