Diagenode

Chromatin shearing with the Bioruptor® Pico 500 µl - 2 ml

Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. No. C01020013). Nuclei are resuspended in buffer iS1 at a final concentration of 1x10e6 per 100 μl. 2 ml nuclei aliquots are sonicated with the Bioruptor® Pico in 15 ml Bioruptor® Tubes (Cat. No. C01020031) with or without sonication beads (sonication beads are part of Cat. No. C01020031) for 10, 20 or 30 cycles (30 sec ON/30 sec OFF).

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