Diagenode

Chromatin shearing with the Bioruptor® Pico 500 µl - 2 ml

Chromatin shearing from HeLa cells is performed according to Diagenode’s Chromatin EasyShear kit - Low SDS (previous name: Chromatin Shearing Optimization kit – Low SDS) protocol (Cat. No. C01020013). Nuclei are resuspended in buffer iS1 at a final concentration of 1x10e6 per 100 μl. 2 ml nuclei aliquots are sonicated with the Bioruptor® Pico in 15 ml Bioruptor® Tubes (Cat. No. C01020031) with or without sonication beads (sonication beads are part of Cat. No. C01020031) for 10, 20 or 30 cycles (30 sec ON/30 sec OFF).

Related product

  • Bioruptor pico next gen sequencing
    B01060010
    Bioruptor® Pico sonication device
  • some alt
    C01020031
    15 ml Bioruptor® Pico Tubes & sonication beads
  • some alt
    B01200016
    15 ml sonication accessories for Bioruptor®...

Events

  • Virtual ChIP workshop - January 18-19, 2022 or January 25-26, 2022
    Diagenode
    Jan 18-Jan 26, 2022
  • Virtual DNA Methylation - January 18-19, 2022 or January 25-26, 2022
    Diagenode
    Jan 18-Jan 26, 2022
  • Lausanne Genomics Days 2022
    Lausanne, Switzerland
    Feb 14-Feb 15, 2022
 See all events

News

 See all news


The European Regional Development Fund and Wallonia are investing in your future.

Extension of industrial buildings and new laboratories.


       Site map   |   Contact us   |   Conditions of sales   |   Conditions of purchase   |   Privacy policy   |   Diagenode Diagnostics