The 0.65 ml Bioruptor® Microtubes for DNA and chromatin shearing strongly improve DNA and chromatin sonication efficiency. These tubes have been thoroughly validated for use on the Bioruptor® Pico (B0106000) or Bioruptor® NGS (UCD-600) and are strongly recommended for DNA and chromatin shearing applications using these instruments. Recommended sample volume: 100 μl.
DNA/RNA shearing
In recent years, advances in Next-Generation Sequencing (NGS) have revolutionized genomics and biology. This growth has fueled demands on upstream techniques for optimal sample preparation and genomic library construction. One of the most critic... Read more
Chromatin shearing
The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, Diagenode's Bio... Read more
Diagenode strongly recommends using this: 0.65 ml Bioruptor® Pico Microtubes (Diagenode Cat# C30010011). Click here to copy to clipboard.
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Restriction-site associated DNA sequencing supports a sister group relationship of Nigritella and Gymnadenia (Orchidaceae). Brandrud MK, Paun O, Lorenz R, Baar J, Hedrén M The orchid genus Nigritella is closely related to Gymnadenia and has from time to time been merged with the latter. Although Nigritella is morphologically distinct, it has been suggested that the separating characters are easily modifiable and subject to rapid evolutionary change. So far, molecular phylogenetic stud...
Epigenome analysis links gene regulatory elements in group 2 innate lymphocytes to asthma susceptibility. Stadhouders R, Li BWS, de Bruijn MJW, Gomez A, Rao TN, Fehling HJ, van IJcken WFJ, Lim AI, Di Santo JP, Graf T, Hendriks RW BACKGROUND: Group 2 innate lymphoid cells (ILC2s) are major producers of the cytokines driving allergic asthma, and increased ILC2 numbers have been detected in blood and sputum of asthmatic patients. Asthma susceptibility has a strong genetic component, but the underlying mechanisms and whether asthma genetics affe...
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