A high quality chromatin preparation is very complex and requires a lot of optimization. Chromatin EasyShear Kit – High SDS is an optimized solution for efficient chromatin preparation prior to ChIP. The protocol, buffers composition, SDS concentration (1%) is optimized for the preparation of chromatin prior to ChIP on low amount of starting materialand it is compatible with Diagenode's True MicroChIP-seq kit and µChIPmentation Kit for Histones. The kit has been validated with the Bioruptor ultrasonicator for efficient chromatin shearing, leading to chromatin fragmentssuitable for ChIPwith the preservedepitopes.
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