Detergents are essential for successful chromatin shearing. Diagenode offers four kits with different SDS concentrations that fit your personal workflow. These kits are compatible with the iDeal ChIP-seq kit for Histones (< 0.1% SDS), theiDeal ChIP-seq kit for Transcription Factors (0.2% SDS), the HighCell# ChIP kit (0.5% SDS) and theTrue MicroChIP kit (1% SDS), which allows you to first optimize your chromatin shearing before performing ChIP using our kits.
Chromatin shearing
The most important steps for a successful ChIP include both cell fixation and lysis, and chromatin shearing. Researchers often overlook the critical nature of both of these steps. Eliminating inconsistencies in the shearing step, Diagenode's Bio... Read more
Diagenode strongly recommends using this: Chromatin Shearing Optimization Kit - High SDS (True Micro ChIP kit) (Diagenode Cat# C01020012). Click here to copy to clipboard.
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